FastQCFastQC Report
Thu 26 May 2016
SRR937433_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937433_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977971
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97380.995735047358255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78740.8051363486238345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51940.5310995929327148No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18920.19346176931626807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14550.1487774177352907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.14489182194564051No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57500.042.490831
GTATCAA92450.035.5280271
ATCAACG119650.027.3977033
TCAACGC120100.027.3741654
TATCAAC119600.027.3694342
CGGTGAC700.0083830427.1481173
CAACGCA122250.026.8927365
AACGCAG125000.026.4070156
GACGAGT2451.0913936E-1125.2012316
CGGTATA950.001225039225.0048452
GAGTACT76350.023.39321912-13
ACGCAGA141850.023.1733027
CGCAGAG142200.023.1162648
GTACATG85650.022.975921
ATATAGG6500.022.6582362
AGTACTT78000.022.5938612-13
GTACTTT79600.022.19598414-15
GCAGAGT147250.021.936379
TACATGG89500.021.8701612
CTTACTA5300.021.5103029
Produced by FastQC (version 0.11.5)