FastQCFastQC Report
Thu 26 May 2016
SRR937432_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937432_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188837
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132061.1108335289026166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98120.8253444332570402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65730.5528932898286308No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.19001763908761252No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.15435253108710445No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18060.15191317228518292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.049.5894551
GTATCAA102400.042.8822821
GTATCGC500.001614970638.0041476
ATCAACG131400.033.334573
TCAACGC132100.033.1994934
TATCAAC132000.033.1124732
CAACGCA133450.032.7239845
AACGCAG137050.032.062976
ACGCAGA154150.028.0759337
CGCAGAG154850.027.8876578
TAGGACG700.00837858527.1515454
GTACATG91750.026.7269571
GCAGAGT160200.026.4214259
TACATGG94250.025.9095212
CCCTATA3850.024.68012
ACATGGG94900.024.629653
GAGTACT94950.024.08929312-13
CATGGGA70700.023.1191394
GGTATGC1652.4267956E-623.0337898
CATGGGT6850.022.8905374
Produced by FastQC (version 0.11.5)