##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937432_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1188837 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.912986389219043 33.0 31.0 34.0 30.0 34.0 2 31.982926170702964 33.0 31.0 34.0 30.0 34.0 3 32.00038188582623 33.0 31.0 34.0 30.0 34.0 4 35.635199779280086 37.0 35.0 37.0 35.0 37.0 5 35.66435348159588 37.0 35.0 37.0 35.0 37.0 6 35.71724130389616 37.0 35.0 37.0 35.0 37.0 7 35.617225069542755 37.0 35.0 37.0 33.0 37.0 8 35.633477928429215 37.0 35.0 37.0 33.0 37.0 9 37.266370410745964 39.0 37.0 39.0 34.0 39.0 10-11 37.169593476649865 39.0 37.0 39.0 34.0 39.0 12-13 37.03698278233265 39.0 37.0 39.0 33.0 39.0 14-15 38.18245604738076 40.0 38.0 41.0 33.0 41.0 16-17 38.14602716772779 40.0 38.0 41.0 33.0 41.0 18-19 38.21502148738641 40.0 38.0 41.0 33.5 41.0 20-21 38.16021750668931 40.0 38.0 41.0 34.0 41.0 22-23 38.01594036861235 40.0 38.0 41.0 33.0 41.0 24-25 37.94870995771498 40.0 38.0 41.0 33.0 41.0 26-27 37.93640549545481 40.0 38.0 41.0 33.0 41.0 28-29 37.845011553307984 40.0 38.0 41.0 33.0 41.0 30-31 37.55325667017429 40.0 37.0 41.0 33.0 41.0 32-33 37.241777047652455 39.0 36.0 41.0 32.5 41.0 34-35 37.20537802911585 40.0 36.0 41.0 32.5 41.0 36-37 37.04455236504248 40.0 36.0 41.0 31.5 41.0 38-39 36.89804153134534 40.0 35.5 41.0 31.0 41.0 40-41 36.63169509360829 39.5 35.0 41.0 30.5 41.0 42-43 36.26442228833726 39.0 35.0 41.0 30.0 41.0 44-45 35.866614178394514 39.0 35.0 41.0 29.5 41.0 46-47 35.29468211369599 39.0 35.0 41.0 25.5 41.0 48-49 34.800530686713145 39.0 35.0 41.0 23.0 41.0 50-51 33.77419234091806 38.0 34.0 39.5 20.5 40.5 52-53 33.85876154594784 38.0 34.0 39.5 20.0 40.5 54-55 34.2179798408024 39.0 34.5 40.0 18.5 41.0 56-57 34.024019272616854 38.5 34.0 40.0 16.5 41.0 58-59 33.78382654644834 38.0 34.0 40.0 13.5 41.0 60-61 33.49935819628763 38.0 34.0 40.0 9.5 41.0 62-63 33.206788651429925 38.0 33.0 40.0 7.5 41.0 64-65 32.802250014089395 37.0 33.0 40.0 4.0 41.0 66-67 32.4652921300397 36.5 32.5 39.5 2.0 41.0 68-69 32.006146763601734 36.0 32.0 39.0 2.0 41.0 70-71 31.56246188501872 35.5 31.5 39.0 2.0 40.0 72-73 31.127696648068657 35.0 31.0 38.0 2.0 40.0 74-75 30.661015765828285 35.0 31.0 37.5 2.0 39.5 76-77 30.201403977164237 35.0 30.0 37.0 2.0 39.0 78-79 29.718036618981408 34.5 30.0 36.0 2.0 39.0 80-81 29.24701536039003 34.0 29.0 36.0 2.0 37.5 82-83 28.78739894535584 34.0 29.0 35.5 2.0 37.0 84-85 28.29027444468838 34.0 28.5 35.0 2.0 37.0 86-87 27.867222756357684 34.0 27.0 35.0 2.0 36.0 88-89 27.44351454404599 34.0 26.5 35.0 2.0 36.0 90-91 26.983908222910287 33.5 25.5 35.0 2.0 35.5 92-93 26.53717078119204 33.0 25.0 35.0 2.0 35.0 94-95 26.07160990110503 33.0 24.5 35.0 2.0 35.0 96-97 25.440610445334393 32.5 21.0 35.0 2.0 35.0 98-99 24.64774439220852 32.0 10.0 34.5 2.0 35.0 100-101 22.25172836982698 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16545889172093098 1101 2 0.12142285519448137 1101 3 0.055561919330433085 1101 4 0.06189344587576784 1101 5 0.10452174844611761 1101 6 -0.045308811906522806 1101 7 0.0747604863269089 1101 8 -0.0878828938793248 1101 9 -0.08600666720973749 1101 10-11 0.017860416600747442 1101 12-13 -0.15627137063635388 1101 14-15 -0.2456718499096482 1101 16-17 -0.09541699180515195 1101 18-19 -0.04403869820885831 1101 20-21 0.030268549085370466 1101 22-23 -0.09312235289061022 1101 24-25 -0.07433390769757864 1101 26-27 -0.20456968255306407 1101 28-29 -0.1490444694215327 1101 30-31 -0.008422778374395534 1101 32-33 -0.07762120598847133 1101 34-35 0.15479468464036472 1101 36-37 0.18053026821025497 1101 38-39 0.187672691253276 1101 40-41 0.29480980556264313 1101 42-43 0.46391468108681977 1101 44-45 0.6273588919935662 1101 46-47 0.6381373064046301 1101 48-49 0.6203278974977309 1101 50-51 0.688528455312138 1101 52-53 0.7033170010757104 1101 54-55 0.6565375187720406 1101 56-57 0.6359863672608341 1101 58-59 0.7096090766729404 1101 60-61 0.7548617995064504 1101 62-63 0.7291520823981514 1101 64-65 0.7031930952355623 1101 66-67 0.6772874869105721 1101 68-69 0.5327468800507624 1101 70-71 0.46935505104594455 1101 72-73 0.4695856373721661 1101 74-75 0.5644206288065838 1101 76-77 0.5677543940917786 1101 78-79 0.4292452841263348 1101 80-81 0.502639029420461 1101 82-83 0.5444462529231728 1101 84-85 0.5624723394208253 1101 86-87 0.5260458723767165 1101 88-89 0.4789937471879213 1101 90-91 0.2951401753753409 1101 92-93 0.4716465220093866 1101 94-95 0.2952578402952213 1101 96-97 0.415650922791972 1101 98-99 0.41003334559689364 1101 100-101 0.4859995552267584 1102 1 0.08160042670483492 1102 2 0.014168041043117086 1102 3 0.017315272709407026 1102 4 0.05064176652064134 1102 5 -0.046164388077457374 1102 6 -0.007118143789746512 1102 7 0.16617604941734498 1102 8 0.11112068874494696 1102 9 0.08353889396143899 1102 10-11 0.010563218724733758 1102 12-13 0.09861875155672095 1102 14-15 0.30470347263316455 1102 16-17 0.3818024226063841 1102 18-19 0.21513777802426404 1102 20-21 0.1505090434022236 1102 22-23 0.10600251649287884 1102 24-25 0.1437610105204925 1102 26-27 0.1867267262926049 1102 28-29 0.1715204336556937 1102 30-31 0.2339947999422023 1102 32-33 0.18195911516820473 1102 34-35 0.3222024000776429 1102 36-37 0.360597122538195 1102 38-39 0.4203753334386775 1102 40-41 0.4203789203559012 1102 42-43 0.5751826208646094 1102 44-45 0.8153520359596129 1102 46-47 0.8796752225115085 1102 48-49 0.8481244236317025 1102 50-51 0.8113826126490551 1102 52-53 0.8887644559197128 1102 54-55 0.9892430931648093 1102 56-57 1.1236177834636791 1102 58-59 0.935523658806737 1102 60-61 1.0978354309653042 1102 62-63 1.0330687117458126 1102 64-65 1.0018037620728322 1102 66-67 0.9786184424902942 1102 68-69 1.1279743127166206 1102 70-71 0.9520430321945135 1102 72-73 1.1064584115602152 1102 74-75 0.8838912854077847 1102 76-77 0.7662488388916451 1102 78-79 0.8449576505776157 1102 80-81 0.9596195489009816 1102 82-83 0.9328664329073924 1102 84-85 0.780560593531245 1102 86-87 0.9344903630256383 1102 88-89 0.9168962592899419 1102 90-91 0.9260072557498091 1102 92-93 1.0041753642672937 1102 94-95 0.8259753780940429 1102 96-97 0.9385701218195415 1102 98-99 0.8698034605394191 1102 100-101 1.424332568682921 1103 1 0.07557709582827243 1103 2 -0.134026230438824 1103 3 -0.08271776665827346 1103 4 -0.01737697273097183 1103 5 -0.034795456943363945 1103 6 -0.04086912861705372 1103 7 0.06148361204389374 1103 8 -0.03683637702033593 1103 9 -0.06707826448350573 1103 10-11 -0.055130057340356586 1103 12-13 -0.04176043872461577 1103 14-15 -0.11883580609202227 1103 16-17 -0.03564575218034349 1103 18-19 -0.08210216402977011 1103 20-21 -0.1314565448255962 1103 22-23 -0.18098674994844544 1103 24-25 -0.2393219860654412 1103 26-27 -0.2310264272726812 1103 28-29 -0.22823897197455523 1103 30-31 -0.24628323904425287 1103 32-33 -0.015561237929446747 1103 34-35 0.18615644994167724 1103 36-37 -0.013086597525173715 1103 38-39 -0.1266506998887209 1103 40-41 0.024808737114490498 1103 42-43 0.024556143623648552 1103 44-45 0.12625248580331316 1103 46-47 0.34026942283173867 1103 48-49 0.407027686057468 1103 50-51 0.3450954751290354 1103 52-53 0.29907104430849785 1103 54-55 0.6035720127774908 1103 56-57 0.4383347038557446 1103 58-59 0.5171436044188553 1103 60-61 0.5129496709327697 1103 62-63 0.541697069689242 1103 64-65 0.26783420912088474 1103 66-67 0.32554451055138856 1103 68-69 0.46867373071545515 1103 70-71 0.5467092922228112 1103 72-73 0.4178586877354391 1103 74-75 0.35313078693691224 1103 76-77 0.4383034639795369 1103 78-79 0.26987780543906226 1103 80-81 0.21182852713735656 1103 82-83 0.20425095184572584 1103 84-85 0.1941965335859699 1103 86-87 0.27060662771333455 1103 88-89 0.31633797869252334 1103 90-91 0.3283801600796643 1103 92-93 0.2627677177715846 1103 94-95 -0.11621420010336436 1103 96-97 -0.22897759465185175 1103 98-99 0.17606569145918627 1103 100-101 0.7989142818164936 1104 1 0.004513952102829677 1104 2 -0.014707431310615249 1104 3 -0.0930269730365687 1104 4 -0.02267621984082524 1104 5 0.0920923862041576 1104 6 0.14105583544013456 1104 7 0.12814286107359862 1104 8 0.04912181805042337 1104 9 0.24462087616143435 1104 10-11 0.19519970025562827 1104 12-13 0.11394317868239767 1104 14-15 0.180074892103967 1104 16-17 0.043056903115527234 1104 18-19 0.09780542094035383 1104 20-21 0.13383524101413258 1104 22-23 -0.05532456222550053 1104 24-25 0.11798078660362421 1104 26-27 0.11068992665332189 1104 28-29 0.1459000912651618 1104 30-31 0.06870191477353416 1104 32-33 0.060614621152907944 1104 34-35 0.18475909803806445 1104 36-37 -0.029294352446449068 1104 38-39 0.014394357892491882 1104 40-41 -0.03014591712545922 1104 42-43 0.03330917340110062 1104 44-45 0.33878305628504535 1104 46-47 0.4730267984717784 1104 48-49 0.6943136381438961 1104 50-51 0.3709954635662527 1104 52-53 0.5005975503615048 1104 54-55 0.5615116113960568 1104 56-57 0.667806720820316 1104 58-59 0.5674877609571709 1104 60-61 0.44209531187114237 1104 62-63 0.70353158220869 1104 64-65 0.8727195957215841 1104 66-67 0.8655844785246529 1104 68-69 0.7175797383468954 1104 70-71 0.8193630827443776 1104 72-73 1.1074454162739222 1104 74-75 0.9193506500596946 1104 76-77 0.7558147595134663 1104 78-79 0.9223355521791241 1104 80-81 0.8589159047190762 1104 82-83 0.9634362771418346 1104 84-85 1.0027669585947514 1104 86-87 1.1674373332396897 1104 88-89 0.823035668534942 1104 90-91 0.7167796303731144 1104 92-93 0.8179712991812416 1104 94-95 0.6592294945779713 1104 96-97 0.7742543134043594 1104 98-99 1.045277262100182 1104 100-101 1.146744732088873 1105 1 0.15201758306393742 1105 2 0.12978350128009453 1105 3 0.07389907972007492 1105 4 0.0858084590578514 1105 5 0.10852298487969136 1105 6 0.12875764803368384 1105 7 0.0769078901434952 1105 8 0.08865643321296801 1105 9 0.141398981132852 1105 10-11 0.034285275457605735 1105 12-13 0.13542514379852122 1105 14-15 0.1449953662830552 1105 16-17 0.06511493631161613 1105 18-19 0.03852881498957572 1105 20-21 -0.02644670811243799 1105 22-23 0.1411519620008903 1105 24-25 0.009219399598684674 1105 26-27 0.1069179465691974 1105 28-29 -0.04192941567919206 1105 30-31 0.048229512298711086 1105 32-33 0.05285524616678572 1105 34-35 0.2538173133374073 1105 36-37 0.219881075163066 1105 38-39 0.28149363081195844 1105 40-41 0.3402794049974247 1105 42-43 0.5011552917149089 1105 44-45 0.48423802570502517 1105 46-47 0.608577794043569 1105 48-49 0.6500607722385041 1105 50-51 0.6470098008658809 1105 52-53 0.6618151104444792 1105 54-55 0.7980874929312307 1105 56-57 0.7506230585907474 1105 58-59 0.7750006303269643 1105 60-61 0.8790722647064086 1105 62-63 0.8050026211299297 1105 64-65 0.6785020400801542 1105 66-67 0.5378219500432593 1105 68-69 0.5981486555432092 1105 70-71 0.7189531283590256 1105 72-73 0.913300924008869 1105 74-75 0.7600114183577382 1105 76-77 0.958518104439225 1105 78-79 0.8918627943808808 1105 80-81 1.0351874073373644 1105 82-83 1.0542547202034775 1105 84-85 1.0684529326472614 1105 86-87 1.032795965599881 1105 88-89 1.1459380389862446 1105 90-91 1.3213424271236853 1105 92-93 1.3518114125294076 1105 94-95 1.2413867074211353 1105 96-97 1.1905627696573795 1105 98-99 1.337266939955093 1105 100-101 1.4291857595065132 1106 1 -0.5013172045866128 1106 2 -0.27037666497133017 1106 3 -0.20940095740328601 1106 4 -0.21690192897694516 1106 5 -0.22973493445162774 1106 6 -0.12795421728123557 1106 7 -0.7058821791662027 1106 8 -0.6298595126098903 1106 9 -0.6667373496248103 1106 10-11 -1.0268285784447428 1106 12-13 -0.6745783580442506 1106 14-15 -0.6860971703906174 1106 16-17 -0.532720905718918 1106 18-19 -0.8137120773488462 1106 20-21 -0.7426611012603033 1106 22-23 -0.5443945168292217 1106 24-25 -0.6383378258702308 1106 26-27 -0.8470763548035976 1106 28-29 -0.5455642519345503 1106 30-31 -0.6394482110059627 1106 32-33 -0.47387835736337536 1106 34-35 -0.12455966864820311 1106 36-37 -0.5233424763561132 1106 38-39 -0.40376198457471446 1106 40-41 -0.3118512654045418 1106 42-43 -0.25426347510062897 1106 44-45 -0.09640195041355071 1106 46-47 -0.02507108785439982 1106 48-49 0.31993554787180756 1106 50-51 0.19929633197487817 1106 52-53 0.26707514619006645 1106 54-55 0.1661546096708122 1106 56-57 0.14159567150125696 1106 58-59 0.1159198544869966 1106 60-61 0.11770180035821909 1106 62-63 0.1401476318650765 1106 64-65 0.44356092079169684 1106 66-67 0.4236346071956305 1106 68-69 0.1076677358023872 1106 70-71 0.30169612371669174 1106 72-73 0.3251118933878381 1106 74-75 0.47156721247234756 1106 76-77 0.2910831372953133 1106 78-79 0.28724524153750153 1106 80-81 0.012751215505268476 1106 82-83 0.09094704791472807 1106 84-85 -0.08809426951431121 1106 86-87 0.02268224182064671 1106 88-89 0.397463636882847 1106 90-91 0.6657606236937887 1106 92-93 0.7497853190168122 1106 94-95 0.8714395029632733 1106 96-97 1.0846238930791614 1106 98-99 0.9837563904145412 1106 100-101 1.2838214913151127 1107 1 0.05359303145734984 1107 2 0.03640235123554092 1107 3 0.18971455131660875 1107 4 0.06866039710471483 1107 5 0.021471043058348016 1107 6 -0.0034788013321573885 1107 7 0.1484909172969111 1107 8 0.09412198064676147 1107 9 0.06534865737179985 1107 10-11 0.034104001053854915 1107 12-13 0.10771548877612958 1107 14-15 0.09032871749550964 1107 16-17 0.10407105399992389 1107 18-19 0.02411600996435226 1107 20-21 0.14663657580499745 1107 22-23 0.16894306634441847 1107 24-25 0.15306982685996928 1107 26-27 0.14006608202537763 1107 28-29 0.08243748967371545 1107 30-31 0.14943675016350966 1107 32-33 0.2218526546877726 1107 34-35 0.26072535804712516 1107 36-37 0.21859477185970633 1107 38-39 0.33762562241671645 1107 40-41 0.41923434067728493 1107 42-43 0.5269143844886699 1107 44-45 0.7334781631358567 1107 46-47 0.8425047141368154 1107 48-49 0.7128681316813186 1107 50-51 0.7000184096618156 1107 52-53 0.5493229201430978 1107 54-55 0.6822529006099245 1107 56-57 0.6857245871561162 1107 58-59 0.4836996164815659 1107 60-61 0.7138168068569257 1107 62-63 0.7128923784804755 1107 64-65 0.8909203796860794 1107 66-67 0.9333371219658986 1107 68-69 0.7868928063396758 1107 70-71 0.8850357437960561 1107 72-73 0.980247051242646 1107 74-75 0.9723833219177713 1107 76-77 0.8692399639470914 1107 78-79 0.9999130168269268 1107 80-81 1.1379699942671309 1107 82-83 1.1241715127689424 1107 84-85 1.1893212420076757 1107 86-87 1.2572683080922928 1107 88-89 1.1312920604471515 1107 90-91 1.3479410935400438 1107 92-93 1.3179925617793309 1107 94-95 1.267195464415103 1107 96-97 1.1755418262557207 1107 98-99 1.1548944602901052 1107 100-101 1.3982387422899194 1108 1 0.06890166461139913 1108 2 0.15493405469048582 1108 3 0.03878734381828153 1108 4 0.11260522678918505 1108 5 0.11508840271527987 1108 6 0.03513419628341552 1108 7 0.08664500741657122 1108 8 0.12972073276872464 1108 9 0.08718133707119335 1108 10-11 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-2.291548112871009 2316 64-65 -2.2123788806812428 2316 66-67 -2.5678723897148217 2316 68-69 -2.2121088130807927 2316 70-71 -2.325433888139546 2316 72-73 -2.3989904510315085 2316 74-75 -2.3152401280093464 2316 76-77 -2.118653688868026 2316 78-79 -2.264404030212635 2316 80-81 -2.1842710949896613 2316 82-83 -2.310173452989634 2316 84-85 -2.1960087401809467 2316 86-87 -1.9488270917203856 2316 88-89 -1.9203732922653245 2316 90-91 -1.7350266909984207 2316 92-93 -1.788976786027714 2316 94-95 -1.7140498047882815 2316 96-97 -1.7626394365328721 2316 98-99 -1.7818481911122284 2316 100-101 -2.063435347656423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4636.0 3 959.0 4 633.0 5 679.0 6 783.0 7 1011.0 8 1184.0 9 1604.0 10 2084.0 11 3050.0 12 4692.0 13 7027.0 14 7806.0 15 11365.0 16 15486.0 17 22272.0 18 17821.0 19 9181.0 20 8215.0 21 8231.0 22 8730.0 23 9450.0 24 10203.0 25 11353.0 26 12748.0 27 14784.0 28 17548.0 29 21120.0 30 25750.0 31 32079.0 32 40460.0 33 53358.0 34 73629.0 35 106723.0 36 155997.0 37 231864.0 38 206791.0 39 27529.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18426359420109 20.01128474931073 15.353162892573065 20.451288763915116 2 14.90041432900455 21.270490904829117 41.35769055002723 22.471404216139103 3 17.848471359488496 28.167300649495175 31.462409539418218 22.521818451598108 4 11.32380106856469 17.604590595720175 39.440441050623186 31.631167285091944 5 11.691617372301176 38.961294086699624 34.43736973809347 14.909718802905726 6 27.803223056092712 38.55084759341828 18.610370147979918 15.035559202509088 7 25.845166992482344 35.711141209470526 21.682710066776753 16.760981731270377 8 26.345103356623778 33.67155917813903 20.570894194590746 19.41244327064645 9 27.501507198718357 15.948228251017097 21.497333417652143 35.05293113261241 10-11 23.52754662501982 28.050190412904225 27.6609317920401 20.761331170035856 12-13 25.500339420413294 26.212321171822623 28.56233318857018 19.725006219193908 14-15 22.59694915997349 26.143053234561574 28.75174742039436 22.50825018507058 16-17 19.500278920562664 30.06152275026216 29.064829740395158 21.373368588780014 18-19 19.474810977990224 26.983750894834873 33.9560850036215 19.585353123553404 20-21 20.868215498747343 25.843231865735472 34.07600605863112 19.212546576886062 22-23 20.380529059667285 26.10119590872665 33.92487303824218 19.593401993363884 24-25 19.741709160822445 26.41885235639701 34.24648872585821 19.59294975692233 26-27 19.630177040842998 26.577584157213753 34.144735399905905 19.647503402037344 28-29 19.35225857374275 26.386906031830094 34.487995621739174 19.772839772687984 30-31 19.44795227347377 26.313636879189804 34.43821866281229 19.80019218452413 32-33 19.28174601166774 26.595206411689997 34.01690287173415 20.106144704908107 34-35 19.69960510974913 26.56462348384703 33.942345601706904 19.793425804696938 36-37 19.95898253068933 26.35070990152435 33.61413226763793 20.07617530014839 38-39 19.51748718997203 26.794299721983926 33.64563594859548 20.042577139448564 40-41 19.56507077116074 26.785339422516767 33.768327197219236 19.881262609103256 42-43 19.63877327593366 26.763861645544377 33.17663931160864 20.42072576691332 44-45 19.65388843187249 27.010667198501643 32.88700797710299 20.448436392522883 46-47 20.08509310484415 27.361186074753746 32.058553164215354 20.495167656186748 48-49 20.11066796321863 27.697310216925064 31.337832469855588 20.854189350000716 50-51 20.27480859375659 27.917268984176406 30.87599034492763 20.931932077139372 52-53 20.35065431345803 28.226499673960998 30.604222394872455 20.818623617708514 54-55 20.128562097373727 28.097784445959682 30.67459258237854 21.099060874288046 56-57 20.212239474653764 28.489191164754118 30.25744596003922 21.041123400552898 58-59 20.315592577981434 27.79379385454705 30.69316737305995 21.19744619441157 60-61 20.431328849579007 28.066214470011978 30.802996062736597 20.699460617672422 62-63 20.411062082488073 27.871093264298935 31.034963838287876 20.68288081492512 64-65 20.278137891388237 27.781673935435798 31.070869234646658 20.86931893852931 66-67 20.203187956235144 27.953242746598782 30.678304701856128 21.165264595309946 68-69 20.290454910029954 27.867259147365598 30.812016911241276 21.03026903136318 70-71 20.29987027032495 28.091279451544406 30.687769585261226 20.92108069286942 72-73 20.329676814048586 28.04769182395769 30.404254296824618 21.218377065169104 74-75 20.00981253340392 28.39548218822867 30.249283870521765 21.345421407845645 76-77 20.013203579637146 28.20777004524292 30.546557373179027 21.23246900194091 78-79 20.03680011803016 28.03587273819435 30.34176054631103 21.58556659746446 80-81 19.821024367831598 28.398661265643504 30.16030247977778 21.620011886747122 82-83 20.169263347433695 28.16499446157581 30.04034535488907 21.62539683610142 84-85 19.929433860788038 27.776724048545727 30.04502374262706 22.24881834803918 86-87 19.95758628800352 27.75088456742819 30.16899727105574 22.12253187351255 88-89 20.04202486506309 27.43035021423408 30.273456682143223 22.254168238559604 90-91 19.623665659702226 27.550538626205977 30.566457545193256 22.25933816889854 92-93 19.737986259895273 27.60434163368066 30.508444571082215 22.149227535341844 94-95 19.355366264569675 27.259714416861016 30.768870873040395 22.616048445528918 96-97 19.554765864258812 27.3945456499883 30.535971351910753 22.514717133842137 98-99 19.421714763654883 27.477127687828666 30.292797635157637 22.808359913358814 100-101 19.416079990289496 27.22844301484224 30.784114894162233 22.571362100706036 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2241.0 1 1412.0 2 652.0 3 702.0 4 597.0 5 603.0 6 1076.0 7 2245.5 8 3696.0 9 4375.0 10 3837.5 11 3189.0 12 3173.5 13 3341.5 14 3509.5 15 3660.0 16 3785.5 17 3913.5 18 4061.5 19 4284.0 20 4581.0 21 5042.0 22 5745.0 23 6507.0 24 7267.0 25 8503.5 26 10460.0 27 12532.5 28 13904.5 29 15835.5 30 18637.0 31 21095.5 32 24320.0 33 28651.5 34 33331.5 35 36388.0 36 40166.0 37 43876.0 38 43988.0 39 43166.0 40 41898.0 41 41051.0 42 40337.0 43 40683.5 44 42058.0 45 42100.0 46 40348.5 47 38590.5 48 39366.5 49 38560.0 50 34719.0 51 30818.0 52 27837.5 53 26589.5 54 25808.0 55 24182.0 56 21724.0 57 18994.5 58 17449.5 59 15871.0 60 13977.0 61 12236.5 62 10026.5 63 7473.0 64 5198.0 65 3744.5 66 2646.5 67 1950.0 68 1393.0 69 901.0 70 692.5 71 555.5 72 427.0 73 360.0 74 293.0 75 225.0 76 172.5 77 117.5 78 70.0 79 45.0 80 36.5 81 27.0 82 18.0 83 11.5 84 6.5 85 2.5 86 1.5 87 2.5 88 2.0 89 2.0 90 1.5 91 0.5 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26631068851322764 2 0.23745896199394873 3 0.22585097872963242 4 0.23215966528632603 5 0.2325802443901056 6 0.25806733807914795 7 0.26033846523955767 8 0.24755286048465855 9 0.24073947900342937 10-11 0.2517586515224543 12-13 0.2516745357016984 14-15 0.2357766455788304 16-17 0.2536512574894624 18-19 0.2409077106449412 20-21 0.232201723196704 22-23 0.23144468080990077 24-25 0.22656596320605768 26-27 0.2340943291637121 28-29 0.2504969142111156 30-31 0.2521371727158559 32-33 0.25259980973001345 34-35 0.2577729327065022 36-37 0.2319073178240583 38-39 0.23169702827216854 40-41 0.226271557833412 42-43 0.23279053394199542 44-45 0.25281009928190323 46-47 0.2588243804659512 48-49 0.26311428732450287 50-51 0.2640395613528179 52-53 0.28565732728708815 54-55 0.26130579717825064 56-57 0.22913149573911307 58-59 0.21272891069170963 60-61 0.20860723547466978 62-63 0.21605148561156828 64-65 0.20885958293693752 66-67 0.21003720442752033 68-69 0.2124345053190639 70-71 0.21230833158793005 72-73 0.21483180621060752 74-75 0.21861701814462367 76-77 0.2351037190127831 78-79 0.22707065813059318 80-81 0.22223399843712804 82-83 0.21693470172950538 84-85 0.21651412262572584 86-87 0.2240845464937582 88-89 0.2216031297814587 90-91 0.2324961285693497 92-93 0.21882730769651348 94-95 0.21849084441348982 96-97 0.22875297454571147 98-99 0.235187834833539 100-101 0.2097007411444967 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1188837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.480317023756086 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2854096083879 35.745751154695895 2 13.072500392430841 12.413729258515845 3 4.585212546991123 6.53122036057328 4 2.117609673663976 4.02179114552553 5 1.1597451948778208 2.753253475978835 6 0.7385872670222557 2.1041014552755772 7 0.5136399785026515 1.7071452310766808 8 0.3522650177621505 1.338052377578078 9 0.2636916631464104 1.1268147386441758 >10 1.644932877949501 15.069356182348246 >50 0.17076432012778925 5.558802049064907 >100 0.09209261192675247 7.763572606944555 >500 0.0023067506871434352 0.7863913231480233 >1k 7.0976944219798E-4 0.5869711108176856 >5k 3.5488472109899E-4 1.380439450372869 >10k+ 1.77442360549495E-4 1.1126080794399822 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13206 1.1108335289026166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9812 0.8253444332570402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6573 0.5528932898286308 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2259 0.19001763908761252 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1835 0.15435253108710445 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1806 0.15191317228518292 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 8.411582075591523E-5 0.0 6 0.0 0.0 0.0 8.411582075591523E-5 0.0 7 0.0 0.0 0.0 8.411582075591523E-5 0.0 8 0.0 0.0 0.0 8.411582075591523E-5 0.0 9 0.0 0.0 0.0 8.411582075591523E-5 0.0 10-11 0.0 0.0 0.0 8.411582075591523E-5 0.0 12-13 0.0 0.0 0.0 8.411582075591523E-5 0.0 14-15 0.0 0.0 0.0 8.411582075591523E-5 0.0 16-17 0.0 0.0 0.0 2.102895518897881E-4 0.0 18-19 0.0 0.0 0.0 3.3646328302366094E-4 0.0 20-21 0.0 0.0 0.0 5.046949245354914E-4 0.0 22-23 0.0 0.0 0.0 8.411582075591524E-4 0.0 24-25 0.0 0.0 0.0 0.0010514477594489404 0.0 26-27 0.0 0.0 0.0 0.0015982005943623893 0.0 28-29 0.0 0.0 0.0 0.004331964768929635 0.0 30-31 0.0 0.0 0.0 0.008663929537859268 0.0 32-33 0.0 0.0 0.0 0.013290299679434608 0.0 34-35 0.0 0.0 0.0 0.018421364745545434 0.0 36-37 0.0 0.0 0.0 0.026202078165467595 0.0 38-39 0.0 0.0 0.0 0.039197972472256495 0.0 40-41 0.0 0.0 0.0 0.05526409423663631 0.0 42-43 0.0 0.0 0.0 0.08033060882189905 0.0 44-45 0.0 0.0 0.0 0.10119133236936603 0.0 46-47 0.0 0.0 0.0 0.1467400493086941 0.0 48-49 0.0 0.0 0.0 0.205410834285945 0.0 50-51 0.0 0.0 0.0 0.23720661453168096 0.0 52-53 0.0 0.0 0.0 0.2872975857918285 0.0 54-55 0.0 0.0 0.0 0.3639270985004672 0.0 56-57 0.0 0.0 0.0 0.479291946667205 0.0 58-59 0.0 0.0 0.0 0.6284292968674428 0.0 60-61 0.0 0.0 0.0 0.8089839061200148 0.0 62-63 0.0 0.0 0.0 0.9975295183443988 0.0 64-65 0.0 0.0 0.0 1.1952016971207997 0.0 66-67 0.0 0.0 0.0 1.4441004107375528 0.0 68-69 0.0 0.0 0.0 1.7473379445626271 0.0 70-71 0.0 0.0 0.0 1.965702615244983 0.0 72-73 0.0 0.0 0.0 2.236134978975251 0.0 74-75 0.0 0.0 0.0 2.5350405480313953 0.0 76-77 0.0 0.0 0.0 2.8616202221162363 0.0 78-79 0.0 0.0 0.0 3.230131632847901 0.0 80-81 0.0 0.0 0.0 3.7536685012327173 0.0 82-83 0.0 0.0 0.0 4.293818244216827 0.0 84-85 0.0 0.0 0.0 4.987647591721994 0.0 86-87 0.0 0.0 0.0 5.791878953969299 0.0 88-89 0.0 0.0 0.0 6.69772222768975 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5635 0.0 49.589455 1 GTATCAA 10240 0.0 42.882282 1 GTATCGC 50 0.0016149706 38.004147 6 ATCAACG 13140 0.0 33.33457 3 TCAACGC 13210 0.0 33.199493 4 TATCAAC 13200 0.0 33.112473 2 CAACGCA 13345 0.0 32.723984 5 AACGCAG 13705 0.0 32.06297 6 ACGCAGA 15415 0.0 28.075933 7 CGCAGAG 15485 0.0 27.887657 8 TAGGACG 70 0.008378585 27.151545 4 GTACATG 9175 0.0 26.726957 1 GCAGAGT 16020 0.0 26.421425 9 TACATGG 9425 0.0 25.909521 2 CCCTATA 385 0.0 24.6801 2 ACATGGG 9490 0.0 24.62965 3 GAGTACT 9495 0.0 24.089293 12-13 CATGGGA 7070 0.0 23.119139 4 GGTATGC 165 2.4267956E-6 23.033789 8 CATGGGT 685 0.0 22.890537 4 >>END_MODULE