FastQCFastQC Report
Thu 26 May 2016
SRR937432_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937432_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1188837
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122561.030923499184497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98750.8306437299646631No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.5502015835644416No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23360.19649455728581797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.15275433049274206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15470.13012717470940088No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG707.922063E-847.4995162
GGTATCA62750.045.5280271
GTATCAA104750.038.3006861
ATCAACG136200.029.4343573
TATCAAC137000.029.2624762
TCAACGC137450.029.1666744
CAACGCA140550.028.590965
AACGCAG143800.027.9105726
CGTATAG1701.11480404E-725.1658551
GTACATG99050.024.8595561
ACGCAGA161850.024.712987
CGCAGAG162350.024.4905728
TACATGG102700.023.7728822
GAGTACT89400.023.75175712-13
ACATGGG101200.023.2803973
GCAGAGT168050.023.1510729
TAACGCA1452.334614E-522.9308014
AGTACTT91750.022.44443912-13
GTACTTT94450.022.27781914-15
CATGGGG23000.022.09764
Produced by FastQC (version 0.11.5)