##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937432_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1188837 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24269937762704 33.0 31.0 34.0 31.0 34.0 2 32.43301815135296 34.0 31.0 34.0 31.0 34.0 3 32.4594843531956 34.0 31.0 34.0 31.0 34.0 4 36.11940324872123 37.0 35.0 37.0 35.0 37.0 5 36.05950437276094 37.0 35.0 37.0 35.0 37.0 6 36.11899612814877 37.0 35.0 37.0 35.0 37.0 7 36.06050703334435 37.0 35.0 37.0 35.0 37.0 8 36.045608439172064 37.0 35.0 37.0 35.0 37.0 9 37.736765427051814 39.0 37.0 39.0 35.0 39.0 10-11 37.66354008160917 39.0 37.0 39.0 35.0 39.0 12-13 37.57684316689336 39.0 37.0 39.0 35.0 39.0 14-15 38.71788941629509 40.0 38.0 41.0 34.5 41.0 16-17 38.70380001631847 40.0 38.0 41.0 34.5 41.0 18-19 38.78523170123407 40.0 38.0 41.0 35.0 41.0 20-21 38.74082822119433 40.0 38.0 41.0 34.5 41.0 22-23 38.58561939105193 40.0 38.0 41.0 34.0 41.0 24-25 38.515433991371395 40.0 38.0 41.0 34.0 41.0 26-27 38.43071842481349 40.0 38.0 41.0 34.0 41.0 28-29 38.2956839331212 40.0 38.0 41.0 34.0 41.0 30-31 38.270124079247196 40.0 37.5 41.0 34.0 41.0 32-33 38.12283265073345 40.0 37.0 41.0 34.0 41.0 34-35 37.9638903398868 40.0 37.0 41.0 33.5 41.0 36-37 37.75732249248635 40.0 37.0 41.0 33.0 41.0 38-39 37.60139699555111 40.0 36.5 41.0 33.0 41.0 40-41 37.37194333621851 40.0 36.0 41.0 33.0 41.0 42-43 37.07584513268009 40.0 36.0 41.0 32.5 41.0 44-45 36.64579248458788 40.0 36.0 41.0 31.0 41.0 46-47 36.08666326838751 39.5 35.0 41.0 29.5 41.0 48-49 35.59405746961106 39.0 35.0 41.0 27.5 41.0 50-51 35.287206740705415 39.0 35.0 41.0 26.0 41.0 52-53 35.03034982928694 39.0 35.0 41.0 25.0 41.0 54-55 34.80113379714797 39.0 35.0 40.0 24.0 41.0 56-57 34.539657244853586 38.0 34.5 40.0 23.0 41.0 58-59 34.27599914874789 38.0 34.0 40.0 23.0 41.0 60-61 33.960461779032784 38.0 34.0 40.0 22.0 41.0 62-63 33.61033724556016 37.5 34.0 40.0 20.5 41.0 64-65 33.22943599501025 37.0 33.0 40.0 18.5 41.0 66-67 32.82222163341147 36.0 33.0 39.0 15.5 41.0 68-69 32.39127988109388 36.0 32.0 39.0 11.5 40.0 70-71 31.993329194834956 35.0 32.0 38.5 7.5 40.0 72-73 31.5335050137235 35.0 31.0 38.0 6.5 40.0 74-75 31.070453308569636 35.0 31.0 37.0 2.0 39.0 76-77 29.81364266085258 34.0 29.5 36.0 2.0 38.5 78-79 30.369709640598334 34.5 31.0 36.0 2.0 39.0 80-81 30.16828589621622 34.5 31.0 36.0 2.0 37.5 82-83 29.825510982582138 34.0 30.5 35.5 2.0 37.0 84-85 29.469211506707815 34.0 30.0 35.0 2.0 37.0 86-87 29.077833210103655 34.0 29.5 35.0 2.0 36.0 88-89 28.63888573454561 34.0 29.0 35.0 2.0 36.0 90-91 28.167770686814087 34.0 29.0 35.0 2.0 35.5 92-93 27.865047521232935 34.0 28.5 35.0 2.0 35.0 94-95 27.48237815613074 34.0 27.0 35.0 2.0 35.0 96-97 27.051981053752534 33.0 26.5 35.0 2.0 35.0 98-99 26.399489164620547 33.0 25.0 35.0 2.0 35.0 100-101 24.10140078076305 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05943608794614619 1101 2 -0.02975242666416733 1101 3 -0.08452269370227583 1101 4 -0.03438315168352801 1101 5 -0.08577168010367586 1101 6 -0.02125971908942148 1101 7 -0.09749528886938208 1101 8 -0.023762598985769046 1101 9 -0.15183253388318718 1101 10-11 -0.042585512297499406 1101 12-13 -0.05134834666640131 1101 14-15 -0.07827476246499998 1101 16-17 -0.11987414838267796 1101 18-19 -0.04941824328065536 1101 20-21 0.04407609902062859 1101 22-23 -0.015987283746525804 1101 24-25 -0.008671632814646557 1101 26-27 -0.11210342199263579 1101 28-29 -0.24886674999772396 1101 30-31 -0.22406389602941346 1101 32-33 -0.2275709862330899 1101 34-35 -0.3147743380530983 1101 36-37 -0.39555080464517545 1101 38-39 -0.32988889585014647 1101 40-41 -0.2453736972088265 1101 42-43 -0.21584218395302202 1101 44-45 -0.31613759744811176 1101 46-47 -0.48986093342966086 1101 48-49 -0.5918520188165033 1101 50-51 -0.5577025912349285 1101 52-53 -0.4985197308164473 1101 54-55 -0.45496109179715205 1101 56-57 -0.3770237397240308 1101 58-59 -0.3085800130273526 1101 60-61 -0.34903676792263383 1101 62-63 -0.4623596223137909 1101 64-65 -0.3554439444062396 1101 66-67 -0.24489635275756427 1101 68-69 -0.5479343152472893 1101 70-71 -0.5986851240065789 1101 72-73 -0.6092377054378062 1101 74-75 -0.7158117153999903 1101 76-77 -0.5330887802408846 1101 78-79 -0.6227508704240101 1101 80-81 -0.6521648468278087 1101 82-83 -0.7675163644698664 1101 84-85 -0.5986256883048426 1101 86-87 -0.676515945384601 1101 88-89 -0.5276062663732617 1101 90-91 -0.5463286367335094 1101 92-93 -0.6128250593904987 1101 94-95 -0.6479339204551202 1101 96-97 -0.7524869507267269 1101 98-99 -0.8369960265854672 1101 100-101 -0.27682802626439695 1102 1 0.02098103187983469 1102 2 0.06835123486314387 1102 3 0.023329224494474943 1102 4 0.019073654236329673 1102 5 0.021053965515079653 1102 6 0.05578269662294133 1102 7 0.0946682536636203 1102 8 0.09006835659395307 1102 9 0.08071816664781295 1102 10-11 0.04817563542177794 1102 12-13 -0.03180918303856117 1102 14-15 0.04195107081501703 1102 16-17 4.4764804749775067E-4 1102 18-19 0.008766080605639104 1102 20-21 0.09915494874233133 1102 22-23 0.18218728388617222 1102 24-25 -0.07295494802161073 1102 26-27 -0.017038593553422743 1102 28-29 -0.086894280759239 1102 30-31 -0.054467687640105567 1102 32-33 0.01396692987380277 1102 34-35 -0.08972209144912568 1102 36-37 -0.09530210014769835 1102 38-39 0.04789136986114784 1102 40-41 0.19512248559031065 1102 42-43 0.1564455242337104 1102 44-45 0.11898464894260741 1102 46-47 0.18708978416145072 1102 48-49 0.055640677321520116 1102 50-51 0.08922232293137 1102 52-53 0.15401369634752626 1102 54-55 0.268475775112627 1102 56-57 0.3199686152116712 1102 58-59 0.3525807678994397 1102 60-61 0.2335728433199833 1102 62-63 0.3691117593423172 1102 64-65 0.46948061789517226 1102 66-67 0.5465970471102253 1102 68-69 0.596287636856438 1102 70-71 0.5896478894495907 1102 72-73 0.6145190386391342 1102 74-75 0.5119954733062109 1102 76-77 0.626587237501635 1102 78-79 0.47925929100733455 1102 80-81 0.3740824075598397 1102 82-83 0.4904312503127244 1102 84-85 0.42636871384473096 1102 86-87 0.3476814294901125 1102 88-89 0.6462922899144559 1102 90-91 0.5734453700478852 1102 92-93 0.5221164564007701 1102 94-95 0.6090074140724191 1102 96-97 0.694854603449798 1102 98-99 0.7290092940380823 1102 100-101 1.282210636222544 1103 1 -4.3525568126767666E-4 1103 2 0.051941682250067345 1103 3 0.008207360057539859 1103 4 -0.017608515518247714 1103 5 -0.05864715528193187 1103 6 -0.01179551772631271 1103 7 -0.03195724086793916 1103 8 -0.10605841291833684 1103 9 -0.07782844003969558 1103 10-11 -0.09386399487616615 1103 12-13 -0.05250114600057998 1103 14-15 -0.13843964088684402 1103 16-17 -0.12197168082975907 1103 18-19 -0.044465423702177986 1103 20-21 -0.04573962178824331 1103 22-23 0.04945797796704454 1103 24-25 -0.06855393481433225 1103 26-27 -0.12100247051142077 1103 28-29 -0.18962129933752436 1103 30-31 -0.23542599873540127 1103 32-33 -0.2393984262312827 1103 34-35 -0.21899920702761477 1103 36-37 -0.32540858857121435 1103 38-39 -0.3701244043190073 1103 40-41 -0.3493069923029637 1103 42-43 -0.3702062323593438 1103 44-45 -0.5648624889162761 1103 46-47 -0.5334479950847424 1103 48-49 -0.4578376903642791 1103 50-51 -0.4383231909155185 1103 52-53 -0.30223455637822383 1103 54-55 -0.3669856323644396 1103 56-57 -0.375734572663724 1103 58-59 -0.3876662649111964 1103 60-61 -0.387499495951964 1103 62-63 -0.26088544501887867 1103 64-65 -0.32234595083941997 1103 66-67 -0.17282671243096104 1103 68-69 -0.36418372478909333 1103 70-71 -0.11567907321592585 1103 72-73 -0.008069722892873443 1103 74-75 -0.027791549846757135 1103 76-77 0.09447127779271725 1103 78-79 -0.039348650301196386 1103 80-81 -0.061007942403307425 1103 82-83 -0.12673566626710908 1103 84-85 -0.12151719020555518 1103 86-87 -0.2002256536420184 1103 88-89 0.019048366193040067 1103 90-91 -0.08463953265541235 1103 92-93 0.013466919375499486 1103 94-95 0.13527794719226804 1103 96-97 0.3864998792438037 1103 98-99 0.47187406136468724 1103 100-101 0.8585612262717675 1104 1 0.08910173105643793 1104 2 0.10572173186096734 1104 3 0.09083852678638493 1104 4 0.0854176621952405 1104 5 0.013328357789468726 1104 6 0.10693030677676063 1104 7 0.03828778113408049 1104 8 0.048606298982825535 1104 9 0.08853519434061496 1104 10-11 0.03836432871606377 1104 12-13 0.0498205743675868 1104 14-15 0.05796673217229653 1104 16-17 -0.055776252275791194 1104 18-19 0.058067917485118414 1104 20-21 -0.05792461765400958 1104 22-23 -0.16731766251319158 1104 24-25 -0.004997439691429406 1104 26-27 -0.16132867200499135 1104 28-29 -0.10000642741176335 1104 30-31 0.015313256053886448 1104 32-33 -0.015662860625553776 1104 34-35 -0.20791114876862338 1104 36-37 -0.12524234623702313 1104 38-39 -0.1161938203109969 1104 40-41 -0.09827190469848546 1104 42-43 0.011163063423296649 1104 44-45 0.12002625743762252 1104 46-47 0.12525431114585928 1104 48-49 0.16394769121859554 1104 50-51 -0.019526898861336406 1104 52-53 -0.08333529006979035 1104 54-55 -8.88814748854827E-4 1104 56-57 -0.06758978911691571 1104 58-59 -0.17675862976293644 1104 60-61 0.13770825242433204 1104 62-63 0.018061774130842423 1104 64-65 0.07718761069533286 1104 66-67 0.1132004230303636 1104 68-69 0.1317780263840902 1104 70-71 0.14392818472367352 1104 72-73 0.19602655803485192 1104 74-75 0.11935167817794579 1104 76-77 0.1557119430238565 1104 78-79 0.09546275627912237 1104 80-81 -0.04719652055138823 1104 82-83 -0.08666908206711099 1104 84-85 0.03550305776168372 1104 86-87 -0.009683358930576702 1104 88-89 0.06456015041834107 1104 90-91 0.04827426785815092 1104 92-93 0.34158063611339884 1104 94-95 0.2825642919771205 1104 96-97 0.21295182713708627 1104 98-99 0.31548823952324057 1104 100-101 0.8642662617564341 1105 1 0.07232913359788995 1105 2 0.08339187625551858 1105 3 0.07244876365385267 1105 4 0.035611091377070636 1105 5 0.028815128654727573 1105 6 0.08148692978306826 1105 7 0.008362887620066317 1105 8 0.0685945733102642 1105 9 0.03542500410934224 1105 10-11 -0.08021864152116365 1105 12-13 0.0333784276812068 1105 14-15 0.08491157265429194 1105 16-17 0.10120540859466587 1105 18-19 0.026413487268420965 1105 20-21 0.06815834147936073 1105 22-23 0.03849920380081784 1105 24-25 -0.03960904899556539 1105 26-27 0.09966281355777795 1105 28-29 0.07643385382672108 1105 30-31 -0.11350858034633404 1105 32-33 0.019619643693843614 1105 34-35 0.013798889561890348 1105 36-37 0.027542945150393905 1105 38-39 -0.2717650693172047 1105 40-41 -0.3395384994121642 1105 42-43 -0.16739516742143934 1105 44-45 -0.21834445147699455 1105 46-47 -0.34071858656097476 1105 48-49 -0.19714594982480804 1105 50-51 -0.2784239749938209 1105 52-53 -0.1100931416067823 1105 54-55 -0.019218839132925325 1105 56-57 0.04759429061765985 1105 58-59 0.09560742441500025 1105 60-61 0.10233958217779104 1105 62-63 0.14731865963231172 1105 64-65 0.11634091942251956 1105 66-67 0.10856500705769179 1105 68-69 0.29217323484790114 1105 70-71 0.3592990313261133 1105 72-73 0.45248011948581635 1105 74-75 0.39564516643228487 1105 76-77 0.4009030848112225 1105 78-79 0.44594111042108153 1105 80-81 0.4623896609211613 1105 82-83 0.15659220944465346 1105 84-85 0.10172357147050448 1105 86-87 0.22307657124837377 1105 88-89 0.44895301374763363 1105 90-91 0.5123508489362898 1105 92-93 0.7070052253817458 1105 94-95 0.97338539996435 1105 96-97 0.7688517502983991 1105 98-99 0.9776227560835338 1105 100-101 1.2760017057108257 1106 1 -0.19136919508545702 1106 2 -0.1156381324321103 1106 3 -0.1242935657796167 1106 4 -0.09286826688632743 1106 5 -0.20527062248098105 1106 6 -0.14844062225661503 1106 7 -0.12210276178998214 1106 8 -0.133143144406759 1106 9 -0.20008923097720555 1106 10-11 -0.343270660151731 1106 12-13 -0.3379498997391792 1106 14-15 -0.37112977588048324 1106 16-17 -0.15813666595057185 1106 18-19 -0.14891019399117056 1106 20-21 -0.025782896450763815 1106 22-23 -0.10596329203715271 1106 24-25 -0.18459634285567716 1106 26-27 -0.19363087049563177 1106 28-29 -0.20361788924900992 1106 30-31 -0.24100005536843838 1106 32-33 -0.19007950399972628 1106 34-35 0.09276353051776454 1106 36-37 -0.19680557814356092 1106 38-39 -0.27832624057836597 1106 40-41 -0.08912325357514561 1106 42-43 -0.2281459204133185 1106 44-45 -0.026474084721151314 1106 46-47 -0.07291803085871607 1106 48-49 -0.2263227106011101 1106 50-51 -0.1562708966252515 1106 52-53 -0.1700320602836598 1106 54-55 -0.06486335042493607 1106 56-57 -0.09319844621598605 1106 58-59 -0.1822020868196077 1106 60-61 -0.04496935689780912 1106 62-63 -0.14834594420019442 1106 64-65 0.08837162465155046 1106 66-67 0.00483391796545618 1106 68-69 0.03304835109759807 1106 70-71 -0.09728146704543761 1106 72-73 -0.01925637354302978 1106 74-75 0.06531805987293282 1106 76-77 0.09888780299899125 1106 78-79 -0.10877374772558213 1106 80-81 -0.18960131848493944 1106 82-83 -0.2816265772720712 1106 84-85 -0.20672945599695325 1106 86-87 -0.0954117234116012 1106 88-89 0.03752093227524966 1106 90-91 0.01688650075744036 1106 92-93 -0.07800602644093857 1106 94-95 -0.1281833490549431 1106 96-97 -0.015259164352315224 1106 98-99 0.20200381015734337 1106 100-101 0.4786641586847118 1107 1 -0.06436219632053053 1107 2 -0.11054640701131291 1107 3 -0.029729409715990585 1107 4 -0.07624862507996966 1107 5 -0.061673080562755445 1107 6 -0.08507345545235978 1107 7 -0.09684728639343376 1107 8 -0.025519620624535833 1107 9 -0.01165766993146633 1107 10-11 -0.07684743425350149 1107 12-13 -0.1221127961544397 1107 14-15 0.07102371843665622 1107 16-17 0.049889604744500105 1107 18-19 0.040294636816327056 1107 20-21 -0.06320699746403591 1107 22-23 0.1522096574238745 1107 24-25 0.03710925852726632 1107 26-27 -0.06048567252779691 1107 28-29 0.051648428090125265 1107 30-31 -0.029434256847508777 1107 32-33 -0.012358402734257368 1107 34-35 -0.20947232376480684 1107 36-37 -0.15315113219269705 1107 38-39 -0.22540252646939507 1107 40-41 -0.18959551393696472 1107 42-43 -0.21654660455498487 1107 44-45 -0.10125385102663387 1107 46-47 -0.2117743783526791 1107 48-49 -0.22399616551651746 1107 50-51 -0.20790376215283146 1107 52-53 -0.06470625236494953 1107 54-55 -0.17321541826144227 1107 56-57 -0.21434449127794863 1107 58-59 -0.3923673779462362 1107 60-61 -0.32527506519134874 1107 62-63 -0.18146344456123842 1107 64-65 -0.30157430574015365 1107 66-67 -0.16177149783274558 1107 68-69 -0.12498535591992521 1107 70-71 -0.006004650481191476 1107 72-73 -0.05919966350140626 1107 74-75 0.10650181463379482 1107 76-77 0.09147438226910865 1107 78-79 0.059723080521234095 1107 80-81 -0.052602973874982695 1107 82-83 -0.26141837102081666 1107 84-85 -0.12775517273988513 1107 86-87 0.044286868429136206 1107 88-89 0.25321092600287187 1107 90-91 0.3853522365546027 1107 92-93 0.4501649723326082 1107 94-95 0.2921854925384544 1107 96-97 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0.1942586232410406 2316 20-21 0.2013851938297222 2316 22-23 0.22027360768677795 2316 24-25 0.34999768921674246 2316 26-27 0.3695771502051812 2316 28-29 0.6040471244678756 2316 30-31 0.6069339737615635 2316 32-33 0.4748108907177624 2316 34-35 0.6498941481671139 2316 36-37 0.6013528107929247 2316 38-39 0.7196865416563156 2316 40-41 0.4987210749031874 2316 42-43 0.4570828962996458 2316 44-45 0.21674103241327458 2316 46-47 0.5103276788279132 2316 48-49 3.5533632189554964E-4 2316 50-51 0.5577687914778338 2316 52-53 0.5586223866704998 2316 54-55 0.8204405413631051 2316 56-57 0.9238340762199968 2316 58-59 0.5973422269466155 2316 60-61 0.8744493413393428 2316 62-63 0.7596860070594218 2316 64-65 0.4469135238995037 2316 66-67 0.031668389324423174 2316 68-69 0.0962542082515796 2316 70-71 0.17888555011581886 2316 72-73 0.09527530510968774 2316 74-75 0.4344299671692795 2316 76-77 0.40929294877877354 2316 78-79 -0.21884419281043677 2316 80-81 0.07987168477768591 2316 82-83 0.018313492480679372 2316 84-85 0.08476737224339104 2316 86-87 -0.048820824154997666 2316 88-89 0.12250505184150384 2316 90-91 0.24469175557998568 2316 92-93 0.1562682334096941 2316 94-95 0.1682874733524784 2316 96-97 0.11581823869914842 2316 98-99 0.1352086288088472 2316 100-101 -0.6350507810388706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 8.0 5 28.0 6 61.0 7 138.0 8 363.0 9 697.0 10 1250.0 11 2117.0 12 3479.0 13 5059.0 14 6367.0 15 9513.0 16 13968.0 17 20832.0 18 18228.0 19 9005.0 20 6945.0 21 6845.0 22 6825.0 23 7060.0 24 7814.0 25 9261.0 26 10711.0 27 12771.0 28 15895.0 29 19595.0 30 24593.0 31 30790.0 32 38718.0 33 51115.0 34 71726.0 35 104424.0 36 160616.0 37 254093.0 38 233194.0 39 24727.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7786994332296 20.905940356527456 15.505753986459128 19.809606223783817 2 15.507088019635997 22.44613853707447 39.99135289362629 22.055420549663243 3 18.52137845642422 28.46613959693381 31.116544993131946 21.895936953510027 4 12.08163945099286 18.324126856751597 38.489296682387916 31.104937009867626 5 12.007886931549578 40.01229809505704 33.60492327570108 14.374891697692298 6 28.528217072651675 38.757962613882306 17.866873255122442 14.846947058343574 7 26.573449514104965 36.08282716638194 20.938194218383178 16.40552910112993 8 27.236282181661576 34.20241799338345 19.88254066789644 18.678759157058536 9 28.0998151975418 16.251681264967356 21.031142200318463 34.61736133717238 10-11 24.24846656729776 28.574210404126386 26.82338018027718 20.353942848298672 12-13 25.94239332552974 26.60147875721808 27.96797694420739 19.488150973044792 14-15 22.990536129006752 26.839045218141766 27.968300111789922 22.20211854106156 16-17 20.049847035379955 30.566553699119392 28.33285807894606 21.050741186554593 18-19 20.051655525526208 27.644370086058895 32.935381385337095 19.368593003077798 20-21 21.433594344725137 26.468388853980823 33.16564844465641 18.93236835663762 22-23 21.002856604510004 26.766493610492386 32.93149364413908 19.29915614085853 24-25 20.468881563286224 27.12417008669876 33.185554861128054 19.221393488886964 26-27 20.322711567861745 27.20034151152496 33.27016211282918 19.206784807784107 28-29 19.923805133605992 27.029325518130136 33.5644358904907 19.482433457773173 30-31 20.04708817508928 27.06829846231621 33.476218494852304 19.408394867742203 32-33 19.936458909000983 27.392359087074176 33.023030070564765 19.648151933360083 34-35 20.342065396686003 27.108888771126736 33.08481314090998 19.46423269127727 36-37 20.575823262566693 26.937208380963916 32.742798213716426 19.74417014275296 38-39 20.151636610479724 27.524353796887524 32.683758555687994 19.640251036944758 40-41 20.247460645312156 27.484710878838996 32.82695545346686 19.440873022381986 42-43 20.23565853908868 27.674651227549468 32.17245507370008 19.91723515966177 44-45 20.269137546058765 27.852825603563826 31.84841960760728 20.029617242770126 46-47 20.69013666297398 28.200796240359278 31.037013484607222 20.072053612059516 48-49 20.68357562895502 28.55626969887377 30.45804429034426 20.302110381826946 50-51 20.88999585309004 28.718739406663822 29.95166704939365 20.439597690852487 52-53 20.959475521034424 29.185582211859153 29.592071915662117 20.262870351444313 54-55 20.722605369785764 29.094400662159742 29.559897614222976 20.623096353831517 56-57 20.778290043126184 29.46509908423106 29.21800886076056 20.5386020118822 58-59 20.902276762920398 28.72092641800348 29.662140394351795 20.714656424724332 60-61 20.845120062716756 29.10491513975423 29.791804932047032 20.25815986548198 62-63 20.82531078692874 28.89773787323241 30.10753366525436 20.169417674584487 64-65 20.662883137049064 28.7933921975847 30.007183491092555 20.53654117427368 66-67 20.60076360342082 29.127332005985686 29.537859269184924 20.73404512140857 68-69 20.706118668917604 28.959647117308762 29.62937728216736 20.704856931606265 70-71 20.672808803898263 29.242612738331665 29.52124639458563 20.56333206318444 72-73 20.750657996007863 29.229616844024875 29.1450383862548 20.87468677371246 74-75 20.532713904429286 29.613269102492605 28.911911389029783 20.942105604048326 76-77 20.62553571263344 29.281306015879384 29.25888914964793 20.834269121839245 78-79 20.687571130440926 29.20581206675095 28.91792567021383 21.188691132594293 80-81 20.39784276475361 29.68074247383892 28.771660358678425 21.149754402729055 82-83 20.623654403504617 29.476086392394553 28.688253970390253 21.21200523371058 84-85 20.393690090706944 29.15534074090586 28.67861091822748 21.772358250159716 86-87 20.211938221976606 29.051291304022335 28.82270656111813 21.914063912882927 88-89 20.406498115385034 28.83082373782108 28.911616983657133 21.851061163136745 90-91 20.10077158105134 29.011809261664567 28.985857151509848 21.901562005774238 92-93 19.89488520102911 29.173815008552772 28.931300631605687 21.99999915881243 94-95 19.730585437700878 28.727697741574325 29.098942916480556 22.44277390424423 96-97 19.98629753279886 28.974451501761806 28.72597336724883 22.3132775981905 98-99 19.65579675858458 29.19200139632791 28.563990301409152 22.58821154367836 100-101 19.344127555383377 29.19230935865193 28.734915661464726 22.728647424499965 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1000.0 1 699.5 2 490.0 3 609.5 4 601.5 5 609.0 6 989.5 7 1893.0 8 2936.5 9 3473.0 10 3253.5 11 3052.0 12 3209.5 13 3373.0 14 3585.0 15 3770.0 16 3939.0 17 3998.5 18 4102.0 19 4368.5 20 4673.0 21 5123.0 22 5762.5 23 6454.5 24 7144.5 25 8286.5 26 10072.0 27 12251.0 28 13674.5 29 15573.5 30 18634.0 31 20858.5 32 23907.5 33 28333.5 34 33258.0 35 36244.0 36 39899.0 37 43703.5 38 44258.0 39 43821.5 40 42068.5 41 41006.5 42 40090.0 43 40198.5 44 41949.5 45 42385.0 46 40254.0 47 38387.0 48 39662.5 49 38527.5 50 34794.5 51 31285.5 52 28130.5 53 26695.0 54 25836.0 55 24358.0 56 21970.5 57 19491.0 58 18134.0 59 16944.5 60 14871.5 61 12673.0 62 10479.5 63 7797.5 64 5332.0 65 3912.5 66 2781.0 67 2005.0 68 1415.5 69 882.0 70 691.0 71 546.5 72 432.5 73 389.0 74 324.0 75 229.0 76 170.5 77 119.0 78 79.0 79 44.5 80 30.0 81 28.5 82 22.0 83 13.0 84 7.0 85 2.5 86 1.5 87 1.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07385369062369358 2 0.0 3 0.0 4 0.0 5 0.0029440537264570333 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.411582075591523E-5 12-13 0.005761933721780194 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001093505669826898 24-25 6.729265660473219E-4 26-27 3.7852119340161855E-4 28-29 0.003616980292504355 30-31 2.9440537264570333E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.729265660473219E-4 40-41 1.2617373113387286E-4 42-43 0.01413145788699376 44-45 2.102895518897881E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.205791037795762E-5 82-83 4.626370141575338E-4 84-85 7.149844764252795E-4 86-87 0.0 88-89 0.0 90-91 0.00916862446239476 92-93 0.003490806561370482 94-95 0.0 96-97 0.0 98-99 3.7852119340161855E-4 100-101 0.012238851919985666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1188837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.59280863496601 #Duplication Level Percentage of deduplicated Percentage of total 1 73.7317494603675 33.616375434677934 2 13.587479969729982 12.389827481826657 3 5.019287225018389 6.86530205802579 4 2.285270164376917 4.167675411345364 5 1.2939427205915648 2.949724142226925 6 0.8119514646461188 2.221148864909452 7 0.5181223404078893 1.653585690100233 8 0.398458688748608 1.4533480596043817 9 0.2823786665745806 1.1586992856958553 >10 1.7858879559366998 15.605148451410242 >50 0.17987832476198476 5.708572557597709 >100 0.10113918393837608 8.290295171942129 >500 0.003340376176059376 1.0121836596062406 >1k 5.567293626765627E-4 0.4821896410679565 >5k 3.711529084510418E-4 1.3889498416865371 >10k+ 1.855764542255209E-4 1.0369742482766933 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12256 1.030923499184497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9875 0.8306437299646631 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6541 0.5502015835644416 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2336 0.19649455728581797 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1816 0.15275433049274206 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1547 0.13012717470940088 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.411582075591523E-5 0.0 12-13 0.0 0.0 0.0 8.411582075591523E-5 0.0 14-15 0.0 0.0 0.0 8.411582075591523E-5 0.0 16-17 0.0 0.0 0.0 2.102895518897881E-4 0.0 18-19 0.0 0.0 0.0 3.3646328302366094E-4 0.0 20-21 0.0 0.0 0.0 5.046949245354914E-4 0.0 22-23 0.0 0.0 0.0 8.411582075591524E-4 0.0 24-25 0.0 0.0 0.0 0.0010514477594489404 0.0 26-27 0.0 0.0 0.0 0.0015982005943623893 0.0 28-29 0.0 0.0 0.0 0.004205791037795762 0.0 30-31 0.0 0.0 0.0 0.008495697896347439 0.0 32-33 0.0 0.0 0.0 0.013080010127544818 0.0 34-35 0.0 0.0 0.0 0.018211075193655647 0.0 36-37 0.0 0.0 0.0 0.02590767279282189 0.0 38-39 0.0 0.0 0.0 0.03898768292036671 0.0 40-41 0.0 0.0 0.0 0.05517997841588039 0.0 42-43 0.0 0.0 0.0 0.08079324583605657 0.0 44-45 0.0 0.0 0.0 0.10148573774201172 0.0 46-47 0.0 0.0 0.0 0.1476653233370092 0.0 48-49 0.0 0.0 0.0 0.20625199249350415 0.0 50-51 0.0 0.0 0.0 0.23830012020150787 0.0 52-53 0.0 0.0 0.0 0.28860138101354516 0.0 54-55 0.0 0.0 0.0 0.36531500954293983 0.0 56-57 0.0 0.0 0.0 0.4814369000964809 0.0 58-59 0.0 0.0 0.0 0.63116306104201 0.0 60-61 0.0 0.0 0.0 0.8123485389502514 0.0 62-63 0.0 0.0 0.0 1.001398846099171 0.0 64-65 0.0 0.0 0.0 1.1989027932340597 0.0 66-67 0.0 0.0 0.0 1.44889501252064 0.0 68-69 0.0 0.0 0.0 1.7540251523127224 0.0 70-71 0.0 0.0 0.0 1.9730627495611257 0.0 72-73 0.0 0.0 0.0 2.245513892989535 0.0 74-75 0.0 0.0 0.0 2.5468167629372234 0.0 76-77 0.0 0.0 0.0 2.8760040274654974 0.0 78-79 0.0 0.0 0.0 3.246534217895304 0.0 80-81 0.0 0.0 0.0 3.7766741782094604 0.0 82-83 0.0 0.0 0.0 4.32094559641061 0.0 84-85 0.0 0.0 0.0 5.021420093755494 0.0 86-87 0.0 0.0 0.0 5.833936864347257 0.0 88-89 0.0 0.0 0.0 6.754626580431127 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 70 7.922063E-8 47.499516 2 GGTATCA 6275 0.0 45.528027 1 GTATCAA 10475 0.0 38.300686 1 ATCAACG 13620 0.0 29.434357 3 TATCAAC 13700 0.0 29.262476 2 TCAACGC 13745 0.0 29.166674 4 CAACGCA 14055 0.0 28.59096 5 AACGCAG 14380 0.0 27.910572 6 CGTATAG 170 1.11480404E-7 25.165855 1 GTACATG 9905 0.0 24.859556 1 ACGCAGA 16185 0.0 24.71298 7 CGCAGAG 16235 0.0 24.490572 8 TACATGG 10270 0.0 23.772882 2 GAGTACT 8940 0.0 23.751757 12-13 ACATGGG 10120 0.0 23.280397 3 GCAGAGT 16805 0.0 23.151072 9 TAACGCA 145 2.334614E-5 22.930801 4 AGTACTT 9175 0.0 22.444439 12-13 GTACTTT 9445 0.0 22.277819 14-15 CATGGGG 2300 0.0 22.0976 4 >>END_MODULE