FastQCFastQC Report
Thu 26 May 2016
SRR937431_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937431_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232839
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65440.5308073479180979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47710.38699294879542256No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34460.27951743901677345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31450.25510224773875584No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25560.2073263418824356No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19640.159307095249258No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18350.1488434418443933No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15800.1281594758115212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT650.00584135829.2337078
CCGTCCG700.00838767827.145599
TACATGG67850.024.1579172
GTATCAA105600.024.1269871
GTACATG69300.024.0069521
GAGTACT52700.022.7139112-13
ACATGGG69750.022.4781193
GTGTACG1302.9275683E-421.9386621
GGTATCA78900.021.8089031
GTCTACG1100.002865638121.6062581
AGTACTT55200.021.34099212-13
GTACTTT56700.021.10894414-15
GTATAGG3650.020.836721
TACCTGG11050.020.6380772
ATCAACG123600.020.603293
TCAACGC123700.020.5866344
CAACGCA123850.020.5633745
CATGGGA46250.020.4423874
TATCAAC124800.020.3290422
GTACCTG11550.020.1658381
Produced by FastQC (version 0.11.5)