##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937431_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1232839 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.729662186222207 33.0 31.0 34.0 30.0 34.0 2 31.831416754336942 33.0 31.0 34.0 30.0 34.0 3 31.869591244274396 33.0 31.0 34.0 30.0 34.0 4 35.4912863723487 37.0 35.0 37.0 33.0 37.0 5 35.501024059102605 37.0 35.0 37.0 33.0 37.0 6 35.550569863542606 37.0 35.0 37.0 33.0 37.0 7 35.49304815957315 37.0 35.0 37.0 33.0 37.0 8 35.48150812879865 37.0 35.0 37.0 33.0 37.0 9 37.070220847977716 39.0 37.0 39.0 33.0 39.0 10-11 36.9630624923449 39.0 37.0 39.0 33.0 39.0 12-13 36.8271870860672 39.0 37.0 39.0 33.0 39.0 14-15 37.91142720176762 40.0 38.0 41.0 33.0 41.0 16-17 37.84225109685855 40.0 38.0 41.0 33.0 41.0 18-19 37.78032168028429 40.0 38.0 41.0 33.0 41.0 20-21 37.65492006661049 40.0 37.0 41.0 32.0 41.0 22-23 37.73872298004849 40.0 38.0 41.0 32.5 41.0 24-25 37.70484142698276 40.0 37.0 41.0 32.0 41.0 26-27 37.616410983104856 40.0 37.0 41.0 32.0 41.0 28-29 37.478216944791654 40.0 37.0 41.0 32.0 41.0 30-31 37.22088082872135 39.5 37.0 41.0 31.5 41.0 32-33 36.956535281573665 39.0 36.0 41.0 31.0 41.0 34-35 36.92098400521074 39.0 36.0 41.0 31.0 41.0 36-37 36.771671726802936 39.0 36.0 41.0 30.5 41.0 38-39 36.616282418061076 39.0 36.0 41.0 30.0 41.0 40-41 36.37959052236342 39.0 35.0 41.0 30.0 41.0 42-43 36.08501150596307 39.0 35.0 40.0 30.0 41.0 44-45 35.78919104603277 39.0 35.0 40.0 28.5 41.0 46-47 35.33678444630645 38.5 35.0 40.0 26.5 41.0 48-49 34.95171956759966 38.0 34.5 40.0 24.5 41.0 50-51 33.97720505272789 37.5 33.5 39.5 23.5 40.5 52-53 34.08189836629114 38.0 33.5 39.5 24.0 40.5 54-55 34.37010915456114 38.0 34.0 40.0 23.0 41.0 56-57 34.08956643973787 38.0 34.0 40.0 22.5 41.0 58-59 33.79886708645654 38.0 33.0 40.0 21.5 41.0 60-61 33.43839868790653 37.5 33.0 40.0 19.5 41.0 62-63 33.04507522880117 37.0 32.5 40.0 18.0 41.0 64-65 32.60771357817201 36.5 32.0 39.0 13.5 41.0 66-67 32.131281132410635 36.0 31.0 39.0 10.0 40.5 68-69 31.611622036616296 35.0 31.0 39.0 7.5 40.0 70-71 31.1048336400779 35.0 30.0 38.0 4.5 40.0 72-73 30.58099354416919 35.0 29.5 37.5 2.0 39.5 74-75 30.002994713827192 34.0 29.0 37.0 2.0 39.0 76-77 29.439825475994837 34.0 29.0 36.0 2.0 39.0 78-79 28.849203342853365 34.0 28.0 36.0 2.0 38.5 80-81 28.2122718376041 34.0 26.5 35.0 2.0 37.0 82-83 27.60853728670167 33.0 26.0 35.0 2.0 37.0 84-85 26.972644441001624 33.0 25.5 35.0 2.0 36.0 86-87 26.344643136695062 32.0 24.5 35.0 2.0 36.0 88-89 25.702824537510573 32.0 21.5 35.0 2.0 35.0 90-91 25.05985250304379 31.5 19.0 34.0 2.0 35.0 92-93 24.32670811030475 31.0 13.5 34.0 2.0 35.0 94-95 23.492087774640485 31.0 4.5 34.0 2.0 35.0 96-97 22.570466216594383 30.5 2.0 34.0 2.0 35.0 98-99 21.323936458856345 29.5 2.0 34.0 2.0 35.0 100-101 18.266171008542074 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32722347028194676 1101 2 0.32870426902263006 1101 3 0.20059675370693952 1101 4 0.10072856367852268 1101 5 0.13172757417852665 1101 6 0.19550544128874492 1101 7 0.18771363102710126 1101 8 0.16300839541612788 1101 9 0.24179435482371758 1101 10-11 0.30100743693598275 1101 12-13 0.2731424649889007 1101 14-15 0.350889733778061 1101 16-17 0.386586963154393 1101 18-19 0.36328792727672976 1101 20-21 0.3250771103989507 1101 22-23 0.3830565743483163 1101 24-25 0.46673670457953165 1101 26-27 0.3546907295958306 1101 28-29 0.3342918525989518 1101 30-31 0.492943494493737 1101 32-33 0.379654430401402 1101 34-35 0.45985765566065595 1101 36-37 0.33924929692891226 1101 38-39 0.4199955734684764 1101 40-41 0.45426411214581464 1101 42-43 0.6692491300666106 1101 44-45 0.6003218077849013 1101 46-47 0.6973697578346574 1101 48-49 0.811085510607235 1101 50-51 0.6982945249869417 1101 52-53 0.7819703968584548 1101 54-55 0.9084285403556009 1101 56-57 0.7752160488932347 1101 58-59 0.8014478959444133 1101 60-61 0.9581081122355855 1101 62-63 0.9629822169233506 1101 64-65 0.8179999163726919 1101 66-67 1.1781558570803412 1101 68-69 1.0005301936697855 1101 70-71 1.1006211308022067 1101 72-73 1.0947258337623893 1101 74-75 1.1100013967456483 1101 76-77 1.076401004432551 1101 78-79 1.310701023017895 1101 80-81 1.4595309481724819 1101 82-83 1.4218525202244692 1101 84-85 1.5249877674493746 1101 86-87 1.4792080073559397 1101 88-89 1.8062891071098406 1101 90-91 1.8159236673196801 1101 92-93 1.9408060262055074 1101 94-95 2.386496108207023 1101 96-97 2.512746105706391 1101 98-99 2.604682616751287 1101 100-101 2.2703438828818285 1102 1 0.21510098558058743 1102 2 0.20983126393427654 1102 3 0.14075082695147856 1102 4 0.21329091622605745 1102 5 0.09186405270921938 1102 6 0.05191561890885765 1102 7 -0.05696442326613038 1102 8 0.12036682113308927 1102 9 0.3427892641927315 1102 10-11 0.24814249172883507 1102 12-13 0.24057403509518593 1102 14-15 0.517509454652604 1102 16-17 0.46127999457791447 1102 18-19 0.39770118454080716 1102 20-21 0.33902200229844226 1102 22-23 0.4839635670520863 1102 24-25 0.3692337085844315 1102 26-27 0.4829975627408132 1102 28-29 0.33015350989352754 1102 30-31 0.4808895221968683 1102 32-33 0.5298024376805586 1102 34-35 0.5703945106681445 1102 36-37 0.6233180005308228 1102 38-39 0.7677719931376785 1102 40-41 0.7510050499008116 1102 42-43 0.9273135635930743 1102 44-45 0.9033190547698453 1102 46-47 0.9562863286181056 1102 48-49 0.9749917646972079 1102 50-51 0.7869910594201102 1102 52-53 0.6786728675159139 1102 54-55 0.8915917876614827 1102 56-57 0.9883453928016053 1102 58-59 1.0105350648912221 1102 60-61 0.7320663993344354 1102 62-63 1.0635224634086669 1102 64-65 0.9488961656030668 1102 66-67 1.0058303730834481 1102 68-69 0.9634162730189821 1102 70-71 0.9754266614148541 1102 72-73 1.2518437263117228 1102 74-75 1.3300095795394746 1102 76-77 1.6886441255936653 1102 78-79 1.832026931518552 1102 80-81 1.7530960742205117 1102 82-83 1.6179189180766862 1102 84-85 1.8343494412461112 1102 86-87 2.0287272824446276 1102 88-89 2.136374076148382 1102 90-91 2.101626938047243 1102 92-93 2.5414461391940684 1102 94-95 2.4324924572469975 1102 96-97 2.119497138229299 1102 98-99 2.422400072505585 1102 100-101 2.3476360040691198 1103 1 0.31817357583229366 1103 2 0.37818718663238826 1103 3 0.30033230626491303 1103 4 0.3109566591234696 1103 5 0.308895928888667 1103 6 0.373896960680284 1103 7 0.2857382772238637 1103 8 0.3006239482789397 1103 9 0.294337089377926 1103 10-11 0.3096686622345288 1103 12-13 0.30187540355630205 1103 14-15 0.3769419983474407 1103 16-17 0.32845234645076715 1103 18-19 0.31270759088738487 1103 20-21 0.36583211927737125 1103 22-23 0.16154183159395075 1103 24-25 0.30190669017591176 1103 26-27 0.32471747917858096 1103 28-29 0.24772723063685476 1103 30-31 0.3112673397272019 1103 32-33 0.33401247852016525 1103 34-35 0.44014031720603697 1103 36-37 0.35999737073032634 1103 38-39 0.4094672120518865 1103 40-41 0.4682047600801482 1103 42-43 0.0632758186816531 1103 44-45 0.4480358997089766 1103 46-47 0.44446426540608996 1103 48-49 0.4032553514052992 1103 50-51 0.41379442210392625 1103 52-53 0.41732633397025864 1103 54-55 0.6951806380258674 1103 56-57 0.6321286440600247 1103 58-59 0.8758056392246303 1103 60-61 0.8427000477317534 1103 62-63 0.8667504024480905 1103 64-65 0.7235837965101766 1103 66-67 0.8708519254251428 1103 68-69 0.947541632736371 1103 70-71 1.109524321699734 1103 72-73 1.292360286615299 1103 74-75 1.0755660720544853 1103 76-77 0.8172749003454314 1103 78-79 1.0727097566423467 1103 80-81 1.0841622840512173 1103 82-83 1.280007332482029 1103 84-85 1.4816340099332024 1103 86-87 1.4028592872968026 1103 88-89 1.721322982293696 1103 90-91 1.5749068783365452 1103 92-93 1.7429219867898844 1103 94-95 1.6035906919880922 1103 96-97 1.7296596915984637 1103 98-99 2.156223194572757 1103 100-101 1.94691970766063 1104 1 0.17476709064818152 1104 2 0.2342905794674479 1104 3 0.1440841671252393 1104 4 0.03743652976790912 1104 5 0.12029286814560436 1104 6 0.1943038993417474 1104 7 0.1486300885223173 1104 8 0.23263670061160013 1104 9 0.2453569553501751 1104 10-11 0.4039369834064246 1104 12-13 0.3296989871266831 1104 14-15 0.5363999784036011 1104 16-17 0.3883319688278064 1104 18-19 0.39516030785797795 1104 20-21 0.45813880738115387 1104 22-23 0.5728744137122845 1104 24-25 0.38190765209755284 1104 26-27 0.44169745746663125 1104 28-29 0.4468518819402618 1104 30-31 0.5296029404472691 1104 32-33 0.33600732078791395 1104 34-35 0.5197370430079786 1104 36-37 0.6161044280287911 1104 38-39 0.5148170995986661 1104 40-41 0.393719960732426 1104 42-43 0.5766339329487877 1104 44-45 0.400006235877683 1104 46-47 0.6367043060816329 1104 48-49 0.5969531614303563 1104 50-51 0.3525589482659015 1104 52-53 0.42546487817055834 1104 54-55 0.668939644589841 1104 56-57 0.7778025159778608 1104 58-59 0.7562353433650131 1104 60-61 0.6096962870761828 1104 62-63 0.5715240531239516 1104 64-65 0.5987538505152088 1104 66-67 0.8230295328053643 1104 68-69 0.8268204663376615 1104 70-71 0.6641328424932702 1104 72-73 0.8692418740608474 1104 74-75 0.9784841300721006 1104 76-77 1.0334587739580847 1104 78-79 1.169822617534738 1104 80-81 1.1411538512048018 1104 82-83 1.3959200358024901 1104 84-85 0.8986682680854869 1104 86-87 0.9496728073345473 1104 88-89 1.1116192541602352 1104 90-91 1.4586712441782517 1104 92-93 1.7319698217790958 1104 94-95 1.5073790600651158 1104 96-97 1.684298215186157 1104 98-99 2.043106934890556 1104 100-101 1.8213396918118256 1105 1 0.17694632007265554 1105 2 0.11778537570749847 1105 3 0.18431634102854844 1105 4 0.15283892348149664 1105 5 0.14807584408531227 1105 6 0.17849988764028524 1105 7 0.15924153631437576 1105 8 0.10839663189990034 1105 9 0.12852866583335043 1105 10-11 0.21001355473080707 1105 12-13 0.34368604130433056 1105 14-15 0.44429254759113235 1105 16-17 0.28473060227102565 1105 18-19 0.2937129796332343 1105 20-21 0.2407072911652932 1105 22-23 0.3452600981342755 1105 24-25 0.2694378214296762 1105 26-27 0.3836211932336653 1105 28-29 0.49808456606314877 1105 30-31 0.4360372252841458 1105 32-33 0.4108148688389264 1105 34-35 0.4296013150013991 1105 36-37 0.6154754504352695 1105 38-39 0.4922142919514556 1105 40-41 0.5248535918804507 1105 42-43 0.5117684693766975 1105 44-45 0.4785992453895176 1105 46-47 0.7115206510220631 1105 48-49 0.7378449420967073 1105 50-51 0.7812253718185431 1105 52-53 0.7971930350942671 1105 54-55 0.9960155905353574 1105 56-57 1.2123456615216028 1105 58-59 1.0076520424202258 1105 60-61 1.1984856911428992 1105 62-63 1.3003051561259298 1105 64-65 1.21645623095349 1105 66-67 1.2110799181890997 1105 68-69 1.2092164511174452 1105 70-71 1.2573646757170387 1105 72-73 1.4954480475578968 1105 74-75 1.3215556199932657 1105 76-77 1.2444853549590427 1105 78-79 1.2823380320841515 1105 80-81 1.2625141382073153 1105 82-83 1.4030687080319915 1105 84-85 1.4663531347166447 1105 86-87 1.6156589146470033 1105 88-89 1.5775536997402817 1105 90-91 1.7215914755844217 1105 92-93 1.9153921636390159 1105 94-95 1.5789305594549354 1105 96-97 1.5036984752541969 1105 98-99 1.7260958300172398 1105 100-101 1.4935455998027614 1106 1 0.1526795519059796 1106 2 -0.6394767769000715 1106 3 0.02750843940665959 1106 4 0.08048556504228799 1106 5 -0.04890049372517069 1106 6 -0.07496370403747932 1106 7 0.022380885445571153 1106 8 0.15854974696976853 1106 9 0.26281336951291934 1106 10-11 -0.23327043191272168 1106 12-13 -0.040234857893473475 1106 14-15 0.1423790417462314 1106 16-17 0.14417952258390443 1106 18-19 0.28255470690194073 1106 20-21 0.16806702219026448 1106 22-23 0.19777126217404373 1106 24-25 0.3720133841491844 1106 26-27 0.3767968555308414 1106 28-29 0.5747156308328485 1106 30-31 0.5044781236361189 1106 32-33 0.6186673747165088 1106 34-35 0.4113692798301045 1106 36-37 0.2742705799727361 1106 38-39 0.5187941261296558 1106 40-41 0.444179347315405 1106 42-43 0.24985951949809504 1106 44-45 0.4487048015538164 1106 46-47 0.424457769075417 1106 48-49 0.43877458990321117 1106 50-51 0.39382646332810367 1106 52-53 0.37647790217347676 1106 54-55 0.5662904094981727 1106 56-57 0.5315401205438519 1106 58-59 0.4131609169427648 1106 60-61 0.4425797555069835 1106 62-63 0.5346351311161754 1106 64-65 0.5666533678074117 1106 66-67 0.5990699488890101 1106 68-69 0.5590252735443322 1106 70-71 0.6869586564589518 1106 72-73 0.7165637979370807 1106 74-75 0.2977502155022762 1106 76-77 0.5674125344698844 1106 78-79 0.8455804191029159 1106 80-81 0.9881459661494212 1106 82-83 1.0289392916762665 1106 84-85 0.9238634625347615 1106 86-87 0.746193116112984 1106 88-89 0.8097157460419702 1106 90-91 0.9116824411224833 1106 92-93 0.9225317665202297 1106 94-95 1.2270994262496266 1106 96-97 1.0749479249818954 1106 98-99 1.0989440009986176 1106 100-101 0.9180371866438968 1107 1 0.1469566045988131 1107 2 0.2455208643898672 1107 3 0.09909983594486604 1107 4 0.15183092833177625 1107 5 -0.004582417662390981 1107 6 0.10743251004567611 1107 7 0.12924810130123632 1107 8 0.047780229847454336 1107 9 0.1561750715495407 1107 10-11 0.17302459618320398 1107 12-13 0.06426402051582158 1107 14-15 -0.008287975785243873 1107 16-17 0.14079977454334625 1107 18-19 0.23155575842665144 1107 20-21 0.21410840070386428 1107 22-23 0.16576768506455153 1107 24-25 0.12265922142707097 1107 26-27 0.40217518513622963 1107 28-29 0.4578712318867275 1107 30-31 0.27223289908798876 1107 32-33 0.1345065673851451 1107 34-35 0.08123437873012307 1107 36-37 0.1743580822325015 1107 38-39 0.322363854626623 1107 40-41 0.4162588524248818 1107 42-43 0.10964303163341782 1107 44-45 -0.05276390997006075 1107 46-47 0.21525088399540238 1107 48-49 0.09421679187939702 1107 50-51 0.05698876076518644 1107 52-53 -5.156357097604314E-4 1107 54-55 0.2458984250474714 1107 56-57 0.18497822125274865 1107 58-59 0.1672528242673863 1107 60-61 0.06321627561271015 1107 62-63 0.17335402529299415 1107 64-65 0.29684849356783616 1107 66-67 0.5938962975523481 1107 68-69 0.6279621852708139 1107 70-71 0.4820434470044326 1107 72-73 0.6779932725063347 1107 74-75 0.6965428340769293 1107 76-77 0.7063902984127495 1107 78-79 0.5491424456894798 1107 80-81 0.7523690947723942 1107 82-83 0.809196835002183 1107 84-85 0.7207779916538648 1107 86-87 0.9751821440998008 1107 88-89 1.1528957578729582 1107 90-91 0.8656004136373561 1107 92-93 1.1620515524071209 1107 94-95 1.40993422134137 1107 96-97 1.378099396956035 1107 98-99 1.7428365465120983 1107 100-101 1.7552658681303264 1108 1 0.0970241203474238 1108 2 0.0869044328433013 1108 3 0.08057463269876308 1108 4 0.1720662064346712 1108 5 0.24909934798078837 1108 6 -0.06016697997038278 1108 7 0.1798721510064425 1108 8 0.07234356521051666 1108 9 0.1445375685262178 1108 10-11 0.13059480709244298 1108 12-13 0.11088695691002926 1108 14-15 0.19752567102535323 1108 16-17 0.13498527694924434 1108 18-19 0.17002648721995683 1108 20-21 0.1766733185242515 1108 22-23 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2316 62-63 -0.6239238212809681 2316 64-65 -0.41010469785486947 2316 66-67 -0.15503832847245036 2316 68-69 -0.20354512419106285 2316 70-71 -0.1690908488566869 2316 72-73 -0.20226191251673242 2316 74-75 -0.14493943898775186 2316 76-77 -0.043790926116830775 2316 78-79 -0.02623521056609235 2316 80-81 0.1479628803156814 2316 82-83 0.3406776895987633 2316 84-85 0.35719328778736426 2316 86-87 0.5641992849380166 2316 88-89 0.6118280603947888 2316 90-91 0.4606264638476496 2316 92-93 0.5685340386771323 2316 94-95 0.9481323491375164 2316 96-97 1.0299185605471806 2316 98-99 1.0948354987601938 2316 100-101 1.7184831626504256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5236.0 3 989.0 4 979.0 5 1033.0 6 1200.0 7 1427.0 8 1787.0 9 2284.0 10 2866.0 11 3781.0 12 4828.0 13 6558.0 14 7463.0 15 9549.0 16 12122.0 17 16090.0 18 13600.0 19 9972.0 20 9816.0 21 10686.0 22 11702.0 23 13154.0 24 14866.0 25 16979.0 26 19311.0 27 22824.0 28 26927.0 29 31631.0 30 37795.0 31 46061.0 32 57381.0 33 73204.0 34 94340.0 35 127000.0 36 168068.0 37 203767.0 38 133080.0 39 12482.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0934215686115 19.25764234772662 14.686552689771206 21.96238339389068 2 14.818862766050014 20.667266811579708 40.9207751790473 23.59309524332298 3 19.320697209567847 25.6236063696733 30.232039957648276 24.823656463110574 4 11.947493482224322 16.587515471077012 38.61662142579494 32.848369620903725 5 12.680002081795028 38.25729360140782 34.59723771822254 14.465466598574622 6 30.39736283746982 36.88962775686727 18.04741039419239 14.665599011470517 7 27.31174823674019 33.78085501435646 21.171149199907653 17.7362475489957 8 26.033903044965594 34.34032790936642 20.217852915544803 19.407916130123176 9 26.165585313104707 16.908467079694944 21.121274208497976 35.80467339870238 10-11 24.243356834404853 27.236145520716367 27.612241263935385 20.9082563809434 12-13 25.62639081648499 24.76988771024348 28.311311987628695 21.29240948564283 14-15 22.481330087947356 25.850572413894024 27.510865376642002 24.157232121516618 16-17 20.978937412178247 29.455105784438018 27.77839876532321 21.78755803806053 18-19 20.813373113558562 27.801736137782314 30.75030105437105 20.634589694288074 20-21 21.966991371682585 26.493596332609464 30.6657042164505 20.87370807925745 22-23 21.449717369003743 26.595641776389055 30.611611199893353 21.34302965471385 24-25 21.286012207052487 26.724113054035126 30.80425348756961 21.18562125134278 26-27 21.014564320158993 26.754942663277063 31.016169707758497 21.21432330880545 28-29 20.80142106311455 27.310670574319246 30.85747501148313 21.030433351083072 30-31 20.984764961851425 26.835595254356353 30.827211023734723 21.3524287600575 32-33 20.865166945318748 26.93425396240819 30.774368236183147 21.42621085608992 34-35 21.396130621502916 26.87553478843211 30.61638114130203 21.11195344876295 36-37 21.273691808950293 26.607823946570075 30.880193989133225 21.238290255346406 38-39 20.981391742270674 27.091945633391152 30.738860160902014 21.187802463436164 40-41 21.177054360848356 27.222526565956883 30.49537767793136 21.1050413952634 42-43 20.874891684102884 27.20919721865911 30.151772316326316 21.76413878091169 44-45 21.11005079492555 27.13529300356597 30.09552599856451 21.659130202943977 46-47 21.502077852820385 27.50041459504632 29.536807910798657 21.46069964133464 48-49 21.375995083118706 27.514251476365146 29.41405538645646 21.69569805405968 50-51 21.137237332951724 27.91726699428564 29.49335059241359 21.45214508034904 52-53 21.4170734335325 28.107146560083923 28.86467826960257 21.61110173678101 54-55 21.192066709103724 27.735056180323355 29.119870054802337 21.953007055770588 56-57 21.52732424428507 27.938358549908415 28.816959323616796 21.71735788218971 58-59 21.803759057216453 27.660396695007446 28.84509340752892 21.69075084024718 60-61 21.4511567877901 27.607836890341158 29.57136697763309 21.369639344235647 62-63 21.2392879828307 27.731582157874822 29.60639597898297 21.422733880311508 64-65 21.211283860113944 27.88651121965492 29.277813447331418 21.624391472899717 66-67 21.186169296240788 27.836233829988522 28.903194501789713 22.074402371980977 68-69 21.143241928542604 27.837856838941473 29.187248220961266 21.831653011554657 70-71 21.306524127107078 27.746569366321815 29.015559412637966 21.931347093933145 72-73 21.328128238452518 27.46378419501062 29.263825322087598 21.944262244449266 74-75 21.09450629481126 28.20355051902017 28.799462569668023 21.90248061650054 76-77 21.18839817472159 27.721039841290036 28.89197699339562 22.19858499059275 78-79 21.30727232008402 27.67073021534925 28.851954352446356 22.17004311212037 80-81 21.310780505369973 27.74759462783282 28.758960453299977 22.182664413497232 82-83 21.311504723278276 27.6605091671628 29.111080857566087 21.916905251992837 84-85 21.18032840175375 27.696392944360245 28.903742772276424 22.219535881609584 86-87 21.075385129888257 27.779455106884694 28.89923821265436 22.245921550572692 88-89 21.154523741887818 27.600622762025175 28.595440800015933 22.64941269607108 90-91 21.075506792933286 27.26650218899436 28.86977342801027 22.78821759006209 92-93 21.25450451091182 27.158416135323698 29.080029212517815 22.50705014124667 94-95 21.032669278670095 27.1458708143363 29.112066488640792 22.709393418352814 96-97 20.72961872256167 27.19333896358409 29.332238170986756 22.744804142867476 98-99 20.865407594426355 27.296788048947423 28.793880434199465 23.043923922426757 100-101 21.109987276604027 26.437477573541514 29.567936879602374 22.88459827025208 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1919.0 1 1115.5 2 321.0 3 337.5 4 317.0 5 315.5 6 506.0 7 984.5 8 1577.0 9 1889.0 10 1808.0 11 1702.0 12 1758.0 13 1895.5 14 2071.5 15 2175.5 16 2254.5 17 2391.0 18 2500.5 19 2700.5 20 3040.5 21 3405.0 22 4011.5 23 4821.5 24 5723.5 25 7023.5 26 8855.0 27 10925.0 28 12732.5 29 14710.5 30 17082.5 31 19716.0 32 22410.0 33 24812.5 34 27540.5 35 30114.5 36 32876.0 37 35790.5 38 37433.0 39 38144.0 40 38908.5 41 40344.5 42 41679.0 43 43058.0 44 44852.5 45 46138.5 46 46927.0 47 49530.5 48 55131.5 49 54228.5 50 46731.0 51 41240.5 52 37825.5 53 36174.5 54 34460.5 55 31168.0 56 27165.5 57 23534.0 58 20627.0 59 17891.0 60 14997.0 61 12208.0 62 9785.5 63 7533.0 64 5405.0 65 3939.0 66 2849.0 67 2070.5 68 1521.0 69 1078.5 70 807.0 71 594.5 72 448.5 73 355.5 74 273.0 75 204.5 76 145.5 77 87.0 78 60.5 79 40.5 80 19.0 81 14.0 82 11.5 83 8.0 84 4.5 85 3.5 86 1.0 87 1.5 88 2.0 89 1.5 90 1.5 91 1.5 92 1.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3002014050496456 2 0.2543722254081839 3 0.2544533390004696 4 0.2534799758930404 5 0.253966657446755 6 0.2213589933478743 7 0.22152122053244583 8 0.22249458363987512 9 0.2351483040364557 10-11 0.2405829147196025 12-13 0.23490496325959836 14-15 0.2259013545158776 16-17 0.22058841422115943 18-19 0.20923251130115122 20-21 0.21458600839201225 22-23 0.21194981664272466 24-25 0.2148293491688696 26-27 0.21142257829286712 28-29 0.22500910500073407 30-31 0.23612166714388497 32-33 0.2279697511191648 34-35 0.22683416082716398 36-37 0.2163299506261564 38-39 0.2048118205215766 40-41 0.20318954867586114 42-43 0.21422099722672627 44-45 0.21073311275843806 46-47 0.22087231179415967 48-49 0.2263880360695922 50-51 0.23969066520445897 52-53 0.2531960783200402 54-55 0.22565801373902028 56-57 0.20525794527914837 58-59 0.19446983750514057 60-61 0.19690324527371375 62-63 0.2030678782874325 64-65 0.19540264381642697 66-67 0.200756140907288 68-69 0.20031001614971622 70-71 0.20201340158771744 72-73 0.20327066226814694 74-75 0.2060285244058632 76-77 0.21661384819915658 78-79 0.21693830256829966 80-81 0.20862415935900794 82-83 0.20250008314143209 84-85 0.189319124394994 86-87 0.19284756565942512 88-89 0.20387901421029023 90-91 0.20112115207257397 92-93 0.20639353557114917 94-95 0.20225674236457478 96-97 0.20927306809729412 98-99 0.21259872538101082 100-101 0.19698435886599955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1232839.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.636754444774475 #Duplication Level Percentage of deduplicated Percentage of total 1 80.3776803007883 49.54219343540275 2 11.752069487374712 14.487188424224836 3 3.4170820944436837 6.318535499185832 4 1.490277335072974 3.674234326260232 5 0.7503334679091586 2.3124059856606443 6 0.4588194136773003 1.6968083721193894 7 0.3273478387416856 1.4123660848184236 8 0.2280429569695161 1.1244662193272268 9 0.17773130226737993 0.9859302571504037 >10 0.9349513959919512 10.439888825381544 >50 0.05872695706589204 2.4849710485793635 >100 0.024428580278134205 2.642455266761434 >500 9.243203123733428E-4 0.40848998655526314 >1k 0.0014525033480152529 1.9374584837513527 >5k 1.3204575891047752E-4 0.5326077848212079 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6544 0.5308073479180979 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4771 0.38699294879542256 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3446 0.27951743901677345 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3145 0.25510224773875584 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2556 0.2073263418824356 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1964 0.159307095249258 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1835 0.1488434418443933 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1580 0.1281594758115212 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.111359228577292E-5 0.0 5 0.0 0.0 0.0 8.111359228577292E-5 0.0 6 0.0 0.0 0.0 8.111359228577292E-5 0.0 7 0.0 0.0 0.0 8.111359228577292E-5 0.0 8 0.0 0.0 0.0 8.111359228577292E-5 0.0 9 0.0 0.0 0.0 8.111359228577292E-5 0.0 10-11 0.0 0.0 0.0 8.111359228577292E-5 0.0 12-13 0.0 0.0 0.0 8.111359228577292E-5 0.0 14-15 0.0 0.0 0.0 8.111359228577292E-5 0.0 16-17 0.0 0.0 0.0 8.111359228577292E-5 0.0 18-19 0.0 0.0 0.0 1.2167038842865939E-4 0.0 20-21 0.0 0.0 0.0 2.027839807144323E-4 0.0 22-23 0.0 0.0 0.0 4.055679614288646E-4 0.0 24-25 0.0 0.0 0.0 6.083519421432969E-4 0.0 26-27 0.0 0.0 0.0 0.0015411582534296856 0.0 28-29 0.0 0.0 0.0 0.002473964564716074 0.0 30-31 0.0 0.0 0.0 0.005677951460004105 0.0 32-33 0.0 0.0 0.0 0.010220312628007388 0.0 34-35 0.0 0.0 0.0 0.015168241757439535 0.0 36-37 0.0 0.0 0.0 0.02040006845987189 0.0 38-39 0.0 0.0 0.0 0.026726928658162178 0.0 40-41 0.0 0.0 0.0 0.03358102720630999 0.0 42-43 0.0 0.0 0.0 0.04376078303817449 0.0 44-45 0.0 0.0 0.0 0.05771232091132743 0.0 46-47 4.055679614288646E-5 0.0 0.0 0.07425949373762511 0.0 48-49 8.111359228577292E-5 0.0 0.0 0.08938717869892177 0.0 50-51 8.111359228577292E-5 0.0 0.0 0.11092283745079447 0.0 52-53 8.111359228577292E-5 0.0 0.0 0.15890152728782914 0.0 54-55 8.111359228577292E-5 0.0 0.0 0.22249458363987512 0.0 56-57 8.111359228577292E-5 0.0 0.0 0.28191028998920376 0.0 58-59 8.111359228577292E-5 0.0 0.0 0.38232891723899065 0.0 60-61 8.111359228577292E-5 0.0 0.0 0.5074871901359382 0.0 62-63 8.111359228577292E-5 0.0 0.0 0.593670381939572 0.0 64-65 8.111359228577292E-5 0.0 0.0 0.6666320582006248 0.0 66-67 1.6222718457154585E-4 0.0 0.0 0.8411479520034651 0.0 68-69 1.6222718457154585E-4 0.0 0.0 1.0260463856188846 0.0 70-71 1.6222718457154585E-4 0.0 0.0 1.1267894672378147 0.0 72-73 1.6222718457154585E-4 0.0 0.0 1.3231654741616707 0.0 74-75 1.6222718457154585E-4 0.0 0.0 1.6054002185200176 0.0 76-77 1.6222718457154585E-4 0.0 0.0 1.8859315774403633 0.0 78-79 2.027839807144323E-4 0.0 0.0 2.17968445190329 0.0 80-81 2.4334077685731875E-4 0.0 0.0 2.5184553700848205 0.0 82-83 2.4334077685731875E-4 0.0 0.0 2.8134655052281765 0.0 84-85 2.4334077685731875E-4 0.0 0.0 3.113464126297108 0.0 86-87 2.4334077685731875E-4 0.0 0.0 3.5722426042654396 0.0 88-89 2.4334077685731875E-4 0.0 0.0 4.17264541436473 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGCGT 65 0.005841358 29.233707 8 CCGTCCG 70 0.008387678 27.14559 9 TACATGG 6785 0.0 24.157917 2 GTATCAA 10560 0.0 24.126987 1 GTACATG 6930 0.0 24.006952 1 GAGTACT 5270 0.0 22.71391 12-13 ACATGGG 6975 0.0 22.478119 3 GTGTACG 130 2.9275683E-4 21.938662 1 GGTATCA 7890 0.0 21.808903 1 GTCTACG 110 0.0028656381 21.606258 1 AGTACTT 5520 0.0 21.340992 12-13 GTACTTT 5670 0.0 21.108944 14-15 GTATAGG 365 0.0 20.83672 1 TACCTGG 1105 0.0 20.638077 2 ATCAACG 12360 0.0 20.60329 3 TCAACGC 12370 0.0 20.586634 4 CAACGCA 12385 0.0 20.563374 5 CATGGGA 4625 0.0 20.442387 4 TATCAAC 12480 0.0 20.329042 2 GTACCTG 1155 0.0 20.165838 1 >>END_MODULE