FastQCFastQC Report
Thu 26 May 2016
SRR937431_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937431_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232839
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80150.65012544217047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66610.5402976382155333No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49710.4032156672525771No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43620.3538174895505415No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18370.14900566902896487No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17330.14056985543124445No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.13489190397124035No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12500.10139199035721616No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG600.003952077631.6650052
TAAGGTG4300.027.614835
GTAAGGT4050.024.6283384
GTACATG68150.024.61751
TACATGG68750.024.1114622
GTATCAA131300.024.0346851
GAGTACT68450.023.80465712-13
ACGTCGC500.001653124623.75260710-11
TACCTGG9000.023.7487532
ACATGGG69400.022.790593
AGTACTT70250.022.78897712-13
GTACTTT73850.021.70671514-15
CGGACGG550.002879167421.58977530-31
GGTATCA98150.021.063671
CAACGCA152700.020.5915855
ATCAACG152950.020.495823
TATCAAC153450.020.490942
TCAACGC153450.020.490944
AGAGTAC112450.020.44688410-11
ATACGTC1404.8537302E-420.3560755
Produced by FastQC (version 0.11.5)