##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937431_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1232839 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.195033576971525 33.0 31.0 34.0 31.0 34.0 2 32.37872990714927 34.0 31.0 34.0 31.0 34.0 3 32.4407680159372 34.0 31.0 34.0 31.0 34.0 4 36.0841877974334 37.0 35.0 37.0 35.0 37.0 5 36.025953916123676 37.0 35.0 37.0 35.0 37.0 6 36.074143501300654 37.0 35.0 37.0 35.0 37.0 7 36.01290598366859 37.0 35.0 37.0 35.0 37.0 8 35.996601340483224 37.0 35.0 37.0 35.0 37.0 9 37.672043145942006 39.0 37.0 39.0 35.0 39.0 10-11 37.596399448752024 39.0 37.0 39.0 35.0 39.0 12-13 37.48950471229415 39.0 37.0 39.0 35.0 39.0 14-15 38.67181237777196 40.0 38.0 41.0 34.0 41.0 16-17 38.64559970928889 40.0 38.0 41.0 34.5 41.0 18-19 38.662730088843716 40.0 38.0 41.0 34.0 41.0 20-21 38.57033359587099 40.0 38.0 41.0 34.0 41.0 22-23 38.51447796508708 40.0 38.0 41.0 34.0 41.0 24-25 38.54065210461383 40.0 38.0 41.0 34.0 41.0 26-27 38.37645061520604 40.0 38.0 41.0 34.0 41.0 28-29 38.21933561478831 40.0 38.0 41.0 34.0 41.0 30-31 38.235703932143615 40.0 38.0 41.0 34.0 41.0 32-33 38.10226274477041 40.0 37.0 41.0 34.0 41.0 34-35 37.951816903910405 40.0 37.0 41.0 33.0 41.0 36-37 37.76998780862708 40.0 37.0 41.0 33.0 41.0 38-39 37.643351646078685 40.0 37.0 41.0 33.0 41.0 40-41 37.447938051927295 40.0 37.0 41.0 33.0 41.0 42-43 37.209484774573156 40.0 36.0 41.0 32.5 41.0 44-45 36.84639316244862 40.0 36.0 41.0 31.0 41.0 46-47 36.416509779460256 39.0 36.0 41.0 30.5 41.0 48-49 36.008943584685426 39.0 35.5 41.0 29.5 41.0 50-51 35.715219911115724 39.0 35.0 40.5 28.0 41.0 52-53 35.48305455943558 39.0 35.0 40.0 28.0 41.0 54-55 35.266416782726694 39.0 35.0 40.0 27.0 41.0 56-57 34.957575563394734 38.0 34.5 40.0 26.5 41.0 58-59 34.69698679227376 38.0 34.0 40.0 26.0 41.0 60-61 34.329399459296795 38.0 34.0 40.0 25.5 41.0 62-63 33.96097908972705 37.0 33.5 40.0 24.5 41.0 64-65 33.54243092569265 37.0 33.0 39.5 23.0 41.0 66-67 33.08802690375629 36.0 32.5 39.0 22.0 40.0 68-69 32.62522438047466 36.0 32.0 39.0 20.5 40.0 70-71 32.17450859357953 35.0 31.5 38.0 19.5 40.0 72-73 31.69740777181773 35.0 31.0 37.5 18.0 39.5 74-75 31.212572769031482 35.0 31.0 37.0 16.5 39.0 76-77 29.917046751441184 33.5 29.5 35.5 13.0 38.0 78-79 30.55460161464717 34.0 30.0 36.0 12.5 38.0 80-81 30.349657173402207 34.0 30.0 36.0 8.0 37.0 82-83 29.942847768443407 34.0 30.0 35.0 7.0 37.0 84-85 29.496695432250277 34.0 29.5 35.0 2.0 36.5 86-87 29.126047277868402 34.0 29.0 35.0 2.0 36.0 88-89 28.653666456041705 34.0 29.0 35.0 2.0 36.0 90-91 28.0918899385889 33.0 27.5 35.0 2.0 35.0 92-93 27.59747339271389 33.0 27.0 35.0 2.0 35.0 94-95 27.096012131348864 33.0 26.0 35.0 2.0 35.0 96-97 26.388869917320918 32.5 25.0 34.0 2.0 35.0 98-99 25.40639815904591 32.0 22.0 34.0 2.0 35.0 100-101 22.470726510112026 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24322488221092442 1101 2 0.22269638961255822 1101 3 0.19080260309609542 1101 4 0.14823165909928804 1101 5 0.15345400774113926 1101 6 0.19927854687520608 1101 7 0.15345472319229714 1101 8 0.2011739289534944 1101 9 0.25098420450432 1101 10-11 0.18162904541967606 1101 12-13 0.2697568086884772 1101 14-15 0.32082223690116507 1101 16-17 0.18713042599892304 1101 18-19 0.21306732818172236 1101 20-21 0.24568670475414223 1101 22-23 0.38263333083828144 1101 24-25 0.30578670972218447 1101 26-27 0.2591318644381815 1101 28-29 0.14096020951055266 1101 30-31 0.2776525602414708 1101 32-33 0.35180998843233624 1101 34-35 0.346511415607317 1101 36-37 0.4583453028431279 1101 38-39 0.33682741859662 1101 40-41 0.4166894714513205 1101 42-43 0.29429512489709 1101 44-45 0.16589415222485115 1101 46-47 0.2634157006152975 1101 48-49 0.23967086667409632 1101 50-51 0.1445610595671596 1101 52-53 0.23621573220205505 1101 54-55 0.2259500179769489 1101 56-57 0.2820432792777936 1101 58-59 0.3464810266974112 1101 60-61 0.24327503615573676 1101 62-63 0.3908054098570517 1101 64-65 0.2703685993900251 1101 66-67 0.19308254060648267 1101 68-69 0.23040088546121495 1101 70-71 0.46376939911378656 1101 72-73 0.13216817305342232 1101 74-75 0.2952106014089395 1101 76-77 0.39550213637922127 1101 78-79 0.19709559020651213 1101 80-81 0.22151804296360922 1101 82-83 0.17639630320934074 1101 84-85 0.42461218183306215 1101 86-87 0.6952368614852773 1101 88-89 0.8353567744335422 1101 90-91 0.8674874360031026 1101 92-93 0.8990408614691638 1101 94-95 0.8989238303502738 1101 96-97 1.064349620789553 1101 98-99 1.3954391615013222 1101 100-101 2.13826242706514 1102 1 0.19369792508352646 1102 2 0.22329046727284663 1102 3 0.16091207499292182 1102 4 0.09802014696678896 1102 5 0.11668435623255391 1102 6 0.13109220223959994 1102 7 0.14736485813441647 1102 8 0.17112065543763322 1102 9 0.270539829862102 1102 10-11 0.21176596780150447 1102 12-13 0.2091688538975589 1102 14-15 0.27557645149521903 1102 16-17 0.3315225946441416 1102 18-19 0.4110144029182905 1102 20-21 0.39766673600581015 1102 22-23 0.289310217461626 1102 24-25 0.24767891535499587 1102 26-27 0.35420193032162217 1102 28-29 0.17651752150261046 1102 30-31 0.17183105440255275 1102 32-33 0.31352839789752807 1102 34-35 0.35748222044598066 1102 36-37 0.40634785897037773 1102 38-39 0.44586001661659225 1102 40-41 0.2970704550481713 1102 42-43 0.23217474564337692 1102 44-45 0.13031749287603844 1102 46-47 0.10172955571270137 1102 48-49 0.17513861178519363 1102 50-51 0.023798272955986022 1102 52-53 0.09234964094854092 1102 54-55 0.2343427875283126 1102 56-57 0.29754762503554133 1102 58-59 0.26107098223263137 1102 60-61 0.2944948869989972 1102 62-63 0.4257825856664823 1102 64-65 0.44886133787180427 1102 66-67 0.39340535694429946 1102 68-69 0.2924563941658178 1102 70-71 0.16009243396293016 1102 72-73 0.23300488932898844 1102 74-75 0.1565094008485488 1102 76-77 0.13660178323305416 1102 78-79 0.17449247164021742 1102 80-81 0.24126372764949622 1102 82-83 0.4160025048338376 1102 84-85 0.5188875834898781 1102 86-87 0.6317746861367688 1102 88-89 0.6445266817824624 1102 90-91 0.7811544088132365 1102 92-93 0.8870426550829436 1102 94-95 0.9789419372000374 1102 96-97 1.118456211068306 1102 98-99 1.602602406531041 1102 100-101 2.2163016976470296 1103 1 0.20969264172379098 1103 2 0.1340607707521997 1103 3 0.2562895445747131 1103 4 0.16242951456008825 1103 5 0.17294081204241252 1103 6 0.16428308134863556 1103 7 0.10939412128858095 1103 8 0.025910815180125724 1103 9 0.16933319891815302 1103 10-11 0.1065053952828876 1103 12-13 0.15406676852600043 1103 14-15 0.2877624961663443 1103 16-17 0.23732780895997507 1103 18-19 0.2891740826418925 1103 20-21 0.28069360172633395 1103 22-23 0.3098893266314988 1103 24-25 0.39909588460044176 1103 26-27 0.23184309850083906 1103 28-29 0.09318248253919847 1103 30-31 0.05703328072738856 1103 32-33 0.12265713476246276 1103 34-35 0.08034735149976768 1103 36-37 0.02020276033719881 1103 38-39 0.1225049603785564 1103 40-41 0.3294446784748004 1103 42-43 0.3715335924736962 1103 44-45 0.40070557147692654 1103 46-47 0.46654677357077645 1103 48-49 0.4964001776627569 1103 50-51 0.3430833546965957 1103 52-53 0.07643270103993416 1103 54-55 0.24633609600037687 1103 56-57 0.2726123366407833 1103 58-59 0.4968521676318929 1103 60-61 0.494692535412689 1103 62-63 0.39880361130961006 1103 64-65 0.5756053446288334 1103 66-67 0.5185719970031357 1103 68-69 0.4286431101729562 1103 70-71 0.3216517619173871 1103 72-73 0.24650358690985996 1103 74-75 0.11968075390442579 1103 76-77 0.09251738592767822 1103 78-79 0.11901310089491446 1103 80-81 0.3712661701117739 1103 82-83 0.2744723909113951 1103 84-85 0.6437987201626996 1103 86-87 0.6317085076896554 1103 88-89 0.627615018411074 1103 90-91 0.8196620780083279 1103 92-93 0.792732227679334 1103 94-95 0.8063615099192347 1103 96-97 1.1408640968101942 1103 98-99 1.5070519860591176 1103 100-101 1.8938028677092618 1104 1 0.1092405385508215 1104 2 0.21670587205170477 1104 3 0.24408688142593604 1104 4 0.06070630064245819 1104 5 0.09994241066457477 1104 6 0.12465878045954071 1104 7 0.06945376043054097 1104 8 0.014562430063342902 1104 9 0.1601216540261845 1104 10-11 0.09342555120580442 1104 12-13 0.09754471074656834 1104 14-15 0.13886504159424362 1104 16-17 0.19845815850397486 1104 18-19 0.1857823927287896 1104 20-21 0.2072039332228499 1104 22-23 0.1614865746194809 1104 24-25 0.019159737875320104 1104 26-27 0.1611842279800726 1104 28-29 0.058944942859930904 1104 30-31 0.18242892826097545 1104 32-33 0.28463443341165373 1104 34-35 0.10880190986321736 1104 36-37 0.20547803024783207 1104 38-39 0.28278286412226095 1104 40-41 0.29942356200440656 1104 42-43 0.23827538064023202 1104 44-45 0.29630729927142596 1104 46-47 0.1962009856424416 1104 48-49 0.1730273139304117 1104 50-51 0.15329850450444837 1104 52-53 0.16576315776949002 1104 54-55 0.2620612246001386 1104 56-57 0.21587155620169085 1104 58-59 0.07819523927673089 1104 60-61 0.048831546832076356 1104 62-63 0.20241356582445036 1104 64-65 0.0933321863652452 1104 66-67 0.25233727896069524 1104 68-69 0.4522983666166809 1104 70-71 0.3211478981987881 1104 72-73 0.46895140266063606 1104 74-75 0.2814874259761737 1104 76-77 0.2154418109504661 1104 78-79 0.3285173448015968 1104 80-81 0.46979213970588773 1104 82-83 0.17964180363532734 1104 84-85 0.3186405756637427 1104 86-87 0.29156843357588613 1104 88-89 0.33963858091079047 1104 90-91 0.6125580576339118 1104 92-93 0.6406501253766805 1104 94-95 0.6630557974788367 1104 96-97 0.7376906714988287 1104 98-99 1.0056171196059047 1104 100-101 1.8690597236912687 1105 1 0.04105424922273215 1105 2 0.1161126418179137 1105 3 -0.010096039493227238 1105 4 0.015866156117560593 1105 5 0.05571564924741068 1105 6 0.05486505951926546 1105 7 -0.047795180343996435 1105 8 0.023624825600279564 1105 9 0.10153996851018121 1105 10-11 0.008268462752276662 1105 12-13 0.020933000647261224 1105 14-15 0.1576987819836475 1105 16-17 0.018023560177944375 1105 18-19 0.11224279633007939 1105 20-21 0.024620494726704578 1105 22-23 -0.035926020286957794 1105 24-25 -0.07359906261282845 1105 26-27 0.10351262066042466 1105 28-29 0.07263330265590895 1105 30-31 -0.055088685510796154 1105 32-33 -0.07789657562355501 1105 34-35 -0.1194621759560377 1105 36-37 -0.20891943880710784 1105 38-39 -0.22658782240298336 1105 40-41 -0.12175430950022559 1105 42-43 -0.25212121512350905 1105 44-45 -0.08589275478946234 1105 46-47 -0.12372508849774277 1105 48-49 -0.23302990423724168 1105 50-51 -0.0771032254438424 1105 52-53 -0.1485992836712171 1105 54-55 -0.22300005773042386 1105 56-57 -0.08782185780711416 1105 58-59 -0.15192698950317407 1105 60-61 -0.07407459355668067 1105 62-63 -0.12287181326549756 1105 64-65 0.22052081246705058 1105 66-67 0.2465041415580771 1105 68-69 0.1413103319857285 1105 70-71 0.14768843346551108 1105 72-73 -0.04161086252238988 1105 74-75 0.18492796940002165 1105 76-77 0.14923162287297131 1105 78-79 0.12818258317330944 1105 80-81 -0.08150971995653933 1105 82-83 0.11958184083817258 1105 84-85 0.14285433367891187 1105 86-87 0.25965931663963104 1105 88-89 0.3434384372815735 1105 90-91 0.4537466133606074 1105 92-93 0.5292795060276774 1105 94-95 0.47850705391456216 1105 96-97 0.8323583969910935 1105 98-99 1.2018165434896204 1105 100-101 1.714755485669837 1106 1 3.456330638513805E-4 1106 2 0.14785446270500557 1106 3 0.12106676998087096 1106 4 0.07459967438083481 1106 5 0.0820720103952084 1106 6 0.026651709576839266 1106 7 0.15422155562333728 1106 8 -0.05834604312829583 1106 9 -0.04241414887226824 1106 10-11 -0.19503720798400792 1106 12-13 -0.2505665466396323 1106 14-15 -0.06432623930636794 1106 16-17 -0.0905593420687083 1106 18-19 0.18771708780042218 1106 20-21 0.2150627694803049 1106 22-23 0.17573321650218787 1106 24-25 0.09535664070726568 1106 26-27 0.18494427059304286 1106 28-29 0.21339487952005953 1106 30-31 0.18770587116677007 1106 32-33 0.16482685415401477 1106 34-35 -0.37230100432298485 1106 36-37 0.22580336812949042 1106 38-39 0.047685673983963284 1106 40-41 0.12237964359830755 1106 42-43 0.21757055549416293 1106 44-45 0.08286158250967901 1106 46-47 -0.2504251655839269 1106 48-49 -0.34796040172184917 1106 50-51 -0.39252699759170184 1106 52-53 -0.39222525613934067 1106 54-55 -0.36165714341142774 1106 56-57 -0.46298838125880337 1106 58-59 -0.2125237441941863 1106 60-61 -0.3793791224178662 1106 62-63 -0.36123679806933495 1106 64-65 -0.4906241960585618 1106 66-67 -0.4114052658395053 1106 68-69 -0.33111790193333945 1106 70-71 -0.3975223232285856 1106 72-73 -0.07130874620876426 1106 74-75 -0.11015447052177763 1106 76-77 -0.19489569812272833 1106 78-79 -0.08959068349297183 1106 80-81 -0.12243844503569079 1106 82-83 -0.2827710177903846 1106 84-85 -0.08240984508413618 1106 86-87 0.0873270079181303 1106 88-89 0.22728965134086465 1106 90-91 0.2977975209975199 1106 92-93 0.4105663069896224 1106 94-95 -0.12255321748262205 1106 96-97 0.4791715274013022 1106 98-99 0.47276870199879895 1106 100-101 1.045967026682181 1107 1 0.06451431345917769 1107 2 0.1142324147028475 1107 3 0.11845774783466823 1107 4 0.06037788289508228 1107 5 0.02602349691739647 1107 6 0.06310848848815453 1107 7 0.06760073671771494 1107 8 0.04479644328371535 1107 9 -0.009437135865667301 1107 10-11 0.0514383657280959 1107 12-13 0.048753759768040084 1107 14-15 0.0936506399865138 1107 16-17 -0.10219938900757342 1107 18-19 -0.017642586066457966 1107 20-21 -0.006789154874091707 1107 22-23 -0.007936487070537623 1107 24-25 -0.1304136305604402 1107 26-27 0.04961742611921238 1107 28-29 0.11666102438229586 1107 30-31 0.08088465283151436 1107 32-33 -0.09120229757682807 1107 34-35 -0.08213473933233928 1107 36-37 -0.11833563851661211 1107 38-39 0.06696612300534355 1107 40-41 0.11404820899983292 1107 42-43 0.052203586206601926 1107 44-45 -0.07380704172587826 1107 46-47 -0.012548988769452762 1107 48-49 -0.10660927269666587 1107 50-51 -0.03674901371650918 1107 52-53 0.0013455741586909653 1107 54-55 0.0566897698875195 1107 56-57 0.13381340044979595 1107 58-59 0.12382617071039448 1107 60-61 0.4065559055497019 1107 62-63 0.07759880144416087 1107 64-65 0.23758233922634986 1107 66-67 0.13050007982116085 1107 68-69 0.08435256177514816 1107 70-71 0.16384425240578082 1107 72-73 0.3679108791146888 1107 74-75 0.1988734521125295 1107 76-77 0.2508512962602225 1107 78-79 0.2249901025939529 1107 80-81 0.07668321626638175 1107 82-83 0.1768827385489793 1107 84-85 0.08754617914693341 1107 86-87 0.2788237779294569 1107 88-89 0.5785807375562655 1107 90-91 0.42500526826361096 1107 92-93 0.5028833304579834 1107 94-95 0.5635185193944494 1107 96-97 0.8971076721458751 1107 98-99 1.3060250162281868 1107 100-101 1.828909541548473 1108 1 0.09962614895167832 1108 2 0.0668267574786654 1108 3 0.062015554320318245 1108 4 0.04203255959127006 1108 5 -0.04095261186621002 1108 6 0.030556295958277246 1108 7 -0.050867220952156345 1108 8 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80-81 0.10760799655611208 2316 82-83 0.30427031189814 2316 84-85 0.34213988375921645 2316 86-87 0.31218823634005943 2316 88-89 0.2593836427181806 2316 90-91 0.5225584542909161 2316 92-93 0.5275729426592477 2316 94-95 0.8224333303770486 2316 96-97 1.2479431324561787 2316 98-99 1.4258490217558588 2316 100-101 1.834057362459479 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 10.0 5 33.0 6 66.0 7 134.0 8 300.0 9 591.0 10 1148.0 11 1793.0 12 2889.0 13 4252.0 14 5442.0 15 7817.0 16 11170.0 17 16152.0 18 14138.0 19 8050.0 20 6571.0 21 6786.0 22 6923.0 23 7617.0 24 8694.0 25 10253.0 26 12475.0 27 15167.0 28 18887.0 29 23797.0 30 29602.0 31 37511.0 32 47856.0 33 63321.0 34 87186.0 35 124231.0 36 187444.0 37 265468.0 38 185650.0 39 13408.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7858120702653 19.985133502285564 15.261936815761734 20.967117611687407 2 15.542106911045897 21.700985855400305 39.65857639040977 23.09833084314403 3 19.920038220724685 25.934935543083892 30.415893721726846 23.72913251446458 4 12.67318765872916 17.3714491511057 37.76502852359473 32.190334666570415 5 12.812289103462597 38.83578622229144 34.0249117479105 14.327012926335462 6 30.64901418595615 37.51025073022512 17.254240010252758 14.586495073565972 7 27.53417112859019 34.22555581061274 20.692726300838956 17.547546759958113 8 26.589603346422365 34.75912102066855 19.501573198122383 19.1497024347867 9 26.5358250347369 17.070274382948625 20.70529890764325 35.688601674671226 10-11 24.711580344229862 27.70661862578974 26.93956794033933 20.642233089641067 12-13 26.251176720384468 25.082466754733957 27.87341834787444 20.792938177007137 14-15 22.73277370362229 26.232581869976535 27.34367585710705 23.690968569294125 16-17 20.94357008498271 29.691387115430317 27.745999274844486 21.619043524742484 18-19 20.865214354834656 27.79049008021323 31.019541075517566 20.32475448943455 20-21 22.083377776984666 26.388416287831014 31.179286433012702 20.348919502171622 22-23 21.61067232950448 26.53214182293952 31.022571589177122 20.834614258378874 24-25 21.45427866435006 26.649365123398205 31.328122591938275 20.568233620313457 26-27 21.328063529659882 26.63163828976079 31.516940972250385 20.52335720832894 28-29 20.893442413510456 27.248602462618436 31.37204243857548 20.48591268529563 30-31 21.124805833708486 26.725879781154816 31.29218427445684 20.857130110679854 32-33 21.084541355713196 26.93958515924076 31.158115708150607 20.81775777689544 34-35 21.546122405277572 26.783221491208504 31.100330213434198 20.570325890079726 36-37 21.62270240587068 26.46632142004001 31.23722206923291 20.673754104856396 38-39 21.17215677479248 27.024180377235137 31.215402504058726 20.58826034391366 40-41 21.36998423962902 27.17362121087993 30.839306673458577 20.617087876032475 42-43 21.07249312408208 27.23439447509196 30.621105995605742 21.072006405220222 44-45 21.405674220234758 27.064198974886423 30.427574079015997 21.102552725862825 46-47 21.86173539286152 27.610539575727245 29.70598756204176 20.82173746936948 48-49 21.820691915164918 27.739956312219192 29.385548315716814 21.053803456899075 50-51 21.520936634872843 28.132262201309338 29.479477855583735 20.867323308234084 52-53 21.91774432833484 28.433923651020127 28.650456385626992 20.997875635018033 54-55 21.50098269117054 28.12285302460419 28.87712831926959 21.499035964955684 56-57 21.829938864685495 28.266991878096004 28.695393315753314 21.207675941465187 58-59 22.199411277547192 27.916500045829178 28.64319671911742 21.24089195750621 60-61 21.660127559235228 28.09170540516645 29.42456395360627 20.823603081992054 62-63 21.470970662024808 27.979363079850657 29.551222827960505 20.998443430164034 64-65 21.50897238001069 28.319837383470187 29.0723281791053 21.09886205741382 66-67 21.589193722781317 28.069034156122573 28.804572210969965 21.53719991012614 68-69 21.504673359619545 28.09198930273945 28.99673031109496 21.406607026546045 70-71 21.729885248600993 28.25425704410714 28.69097262497374 21.32488508231813 72-73 21.74792774713932 27.776989172129003 29.041227672433585 21.433855408298097 74-75 21.489058993104535 28.796947533295103 28.314808340748463 21.399185132851898 76-77 21.615515083478055 28.076861617778153 28.7302721604362 21.577351138307595 78-79 21.722868922868273 28.073495403698296 28.4847818733833 21.718853800050127 80-81 21.694884733529683 28.185026593091234 28.493947709311595 21.62614096406749 82-83 21.67120295351411 28.13235980602456 28.775794681614308 21.420642558847018 84-85 21.40781790293445 28.308162392919744 28.49066827163688 21.79335143250892 86-87 21.24677269294693 28.256893235856428 28.490338154454882 22.005995916741764 88-89 21.451300615895505 28.201330425140668 28.2006004028101 22.146768556153724 90-91 21.384458757868778 27.92316990070738 28.400204425984814 22.29216691543903 92-93 21.29188494893645 27.9801740147539 28.552030638536728 22.17591039777292 94-95 21.25382957547579 27.883486813768872 28.681563448268587 22.18112016248675 96-97 20.94438122090557 27.941442475457055 28.87854780713459 22.235628496502787 98-99 21.024199072626416 28.17052382858554 28.125667921090912 22.679609177697124 100-101 20.748511786456568 28.151289136081697 28.534679553430475 22.56551952403126 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 488.0 1 353.0 2 259.5 3 328.0 4 326.0 5 325.0 6 547.0 7 1102.0 8 1795.5 9 2249.0 10 2206.5 11 2038.0 12 2093.0 13 2285.5 14 2494.5 15 2710.5 16 2807.5 17 2833.5 18 2972.5 19 3174.5 20 3426.0 21 3769.0 22 4306.5 23 5043.5 24 5858.0 25 7058.5 26 8876.0 27 10945.5 28 12805.5 29 14725.5 30 17192.0 31 19950.5 32 22430.0 33 24515.5 34 27213.5 35 29734.5 36 32559.0 37 35674.5 38 37311.5 39 38037.0 40 38970.0 41 40341.5 42 41140.5 43 42277.0 44 44528.5 45 46091.5 46 46319.0 47 49281.0 48 55972.5 49 54334.5 50 46032.0 51 40975.5 52 37699.0 53 35822.5 54 34275.0 55 31354.5 56 26970.5 57 23378.0 58 21099.5 59 18302.0 60 15016.0 61 12142.0 62 9653.0 63 7205.5 64 5153.5 65 3756.5 66 2710.0 67 2028.0 68 1470.0 69 1000.5 70 738.5 71 547.5 72 422.0 73 359.5 74 280.5 75 225.0 76 163.5 77 91.5 78 48.5 79 31.5 80 19.5 81 13.5 82 9.5 83 5.0 84 2.5 85 0.5 86 0.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04363911264974583 2 8.111359228577292E-5 3 0.0 4 0.0 5 5.677951460004104E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005637394663861218 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.6501116528597815E-4 22-23 4.8668155371463755E-4 24-25 8.111359228577292E-5 26-27 0.002352294176287415 28-29 0.0036906684490026678 30-31 3.244543691430917E-4 32-33 3.6501116528597815E-4 34-35 0.0 36-37 4.055679614288646E-5 38-39 4.055679614288646E-5 40-41 0.0 42-43 0.007665234471005541 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.6222718457154585E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.027839807144323E-4 84-85 1.6222718457154585E-4 86-87 0.0 88-89 0.0 90-91 0.009652517482006977 92-93 0.0010139199035721616 94-95 0.0 96-97 0.0 98-99 2.027839807144323E-4 100-101 0.012410379619723258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1232839.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.207953139349534 #Duplication Level Percentage of deduplicated Percentage of total 1 79.01619259605063 46.78387028476783 2 12.286272921969065 14.548901428424067 3 3.755473077537079 6.670616219727125 4 1.661613153402951 3.9352285488963488 5 0.8533686060594422 2.5263104219079744 6 0.5256970611379195 1.8675268176808622 7 0.36127555697731734 1.4973270373533785 8 0.2438469558619153 1.1550143308676253 9 0.19276403899432493 1.027184776895496 >10 1.006595757472525 10.835069496981518 >50 0.06393751828518453 2.600397312448291 >100 0.02950029212795571 2.998579474202689 >500 0.0018004813443123872 0.6801259600976073 >1k 0.0013849856494710672 1.6703841098611356 >5k 2.7699712989421346E-4 1.2034637798881702 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8015 0.65012544217047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6661 0.5402976382155333 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4971 0.4032156672525771 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4362 0.3538174895505415 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1837 0.14900566902896487 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1733 0.14056985543124445 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1663 0.13489190397124035 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1250 0.10139199035721616 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.111359228577292E-5 0.0 5 0.0 0.0 0.0 8.111359228577292E-5 0.0 6 0.0 0.0 0.0 8.111359228577292E-5 0.0 7 0.0 0.0 0.0 8.111359228577292E-5 0.0 8 0.0 0.0 0.0 8.111359228577292E-5 0.0 9 0.0 0.0 0.0 2.4334077685731875E-4 0.0 10-11 0.0 0.0 0.0 2.4334077685731875E-4 0.0 12-13 0.0 0.0 0.0 3.244543691430917E-4 0.0 14-15 0.0 0.0 0.0 3.244543691430917E-4 0.0 16-17 0.0 0.0 0.0 3.244543691430917E-4 0.0 18-19 0.0 0.0 0.0 3.6501116528597815E-4 0.0 20-21 0.0 0.0 0.0 4.4612475757175104E-4 0.0 22-23 0.0 0.0 0.0 6.489087382861834E-4 0.0 24-25 0.0 0.0 0.0 7.705791267148428E-4 0.0 26-27 0.0 0.0 0.0 0.0017033854380012312 0.0 28-29 0.0 0.0 0.0 0.0025956349531447336 0.0 30-31 0.0 0.0 0.0 0.005961849033004309 0.0 32-33 0.0 0.0 0.0 0.010666437385579139 0.0 34-35 0.0 0.0 0.0 0.01569548010729706 0.0 36-37 0.0 0.0 0.0 0.020886750013586527 0.0 38-39 0.0 0.0 0.0 0.02737583739644836 0.0 40-41 0.0 0.0 0.0 0.03427049274073906 0.0 42-43 0.0 0.0 0.0 0.04445024857260356 0.0 44-45 0.0 0.0 0.0 0.05823955926118496 0.0 46-47 0.0 0.0 0.0 0.07474617529133974 0.0 48-49 0.0 0.0 0.0 0.09007664423335082 0.0 50-51 0.0 0.0 0.0 0.11218009813122395 0.0 52-53 0.0 0.0 0.0 0.1608482535026877 0.0 54-55 0.0 0.0 0.0 0.22488743461230543 0.0 56-57 0.0 0.0 0.0 0.2846275953307772 0.0 58-59 0.0 0.0 0.0 0.38557346093042155 0.0 60-61 0.0 0.0 0.0 0.5119889945077987 0.0 62-63 0.0 0.0 0.0 0.5991049926227188 0.0 64-65 0.0 0.0 0.0 0.6734861567487725 0.0 66-67 0.0 0.0 0.0 0.8498271063780429 0.0 68-69 0.0 0.0 0.0 1.036956163781321 0.0 70-71 0.0 0.0 0.0 1.1394026308382523 0.0 72-73 0.0 0.0 0.0 1.3359814217428228 0.0 74-75 0.0 0.0 0.0 1.6200412219275997 0.0 76-77 0.0 0.0 0.0 1.9034521133740903 0.0 78-79 0.0 0.0 0.0 2.203694075219879 0.0 80-81 0.0 0.0 0.0 2.5491568647649854 0.0 82-83 0.0 0.0 0.0 2.849033815445488 0.0 84-85 0.0 0.0 0.0 3.153250343313279 0.0 86-87 0.0 0.0 4.055679614288646E-5 3.62001851012176 0.0 88-89 0.0 0.0 8.111359228577292E-5 4.235143437220919 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTACG 60 0.0039520776 31.665005 2 TAAGGTG 430 0.0 27.61483 5 GTAAGGT 405 0.0 24.628338 4 GTACATG 6815 0.0 24.6175 1 TACATGG 6875 0.0 24.111462 2 GTATCAA 13130 0.0 24.034685 1 GAGTACT 6845 0.0 23.804657 12-13 ACGTCGC 50 0.0016531246 23.752607 10-11 TACCTGG 900 0.0 23.748753 2 ACATGGG 6940 0.0 22.79059 3 AGTACTT 7025 0.0 22.788977 12-13 GTACTTT 7385 0.0 21.706715 14-15 CGGACGG 55 0.0028791674 21.589775 30-31 GGTATCA 9815 0.0 21.06367 1 CAACGCA 15270 0.0 20.591585 5 ATCAACG 15295 0.0 20.49582 3 TATCAAC 15345 0.0 20.49094 2 TCAACGC 15345 0.0 20.49094 4 AGAGTAC 11245 0.0 20.446884 10-11 ATACGTC 140 4.8537302E-4 20.356075 5 >>END_MODULE