FastQCFastQC Report
Thu 26 May 2016
SRR937430_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937430_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236377
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66560.5383471222774283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49680.40181918621909013No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33770.2731367535953839No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.25768839116224257No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT25080.20285074859852617No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18210.14728517272644184No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17060.13798380267507404No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16100.1302191807191496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67250.026.8010771
TACATGG66700.026.4431112
GTACCGT908.94777E-426.3889686
GTATCAA106050.025.7847251
ACATGGG68100.024.7127253
GAGTACT55850.022.5806412-13
CATGGGA46750.022.373854
ATCAACG122250.022.1272723
TCAACGC122300.022.0422694
AGTACTT59100.021.94169212-13
CAACGCA123250.021.7943345
TATCAAC125050.021.5938012
GGTATCA77300.021.532471
AACGCAG125200.021.359896
AGAGTAC94950.020.88605510-11
ATGGGAT19200.020.7998475
ACCGTCC1150.003723367120.6522378
GTACTTT60800.020.58614-15
TGTACCG1404.8228365E-420.375365
CATGGGG22700.019.6880054
Produced by FastQC (version 0.11.5)