##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937430_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1236377 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72071706283763 33.0 31.0 34.0 30.0 34.0 2 31.820216649128866 33.0 31.0 34.0 30.0 34.0 3 31.852713209643984 33.0 31.0 34.0 30.0 34.0 4 35.470484326382646 37.0 35.0 37.0 33.0 37.0 5 35.488613909834946 37.0 35.0 37.0 33.0 37.0 6 35.54559329395484 37.0 35.0 37.0 33.0 37.0 7 35.488857363085856 37.0 35.0 37.0 33.0 37.0 8 35.480010546944825 37.0 35.0 37.0 33.0 37.0 9 37.10221558634623 39.0 37.0 39.0 33.0 39.0 10-11 36.984355095573605 39.0 37.0 39.0 33.0 39.0 12-13 36.858692372957435 39.0 37.0 39.0 33.0 39.0 14-15 38.017519737102845 40.0 38.0 41.0 33.0 41.0 16-17 37.99145972466327 40.0 38.0 41.0 33.0 41.0 18-19 37.99487090102776 40.0 38.0 41.0 33.0 41.0 20-21 37.94489666177873 40.0 38.0 41.0 33.0 41.0 22-23 37.78822721548525 40.0 38.0 41.0 33.0 41.0 24-25 37.630543919856166 40.0 37.0 41.0 32.0 41.0 26-27 37.470960313884845 40.0 37.0 41.0 32.0 41.0 28-29 37.53717636287314 40.0 37.0 41.0 32.0 41.0 30-31 37.38103426382082 40.0 37.0 41.0 32.0 41.0 32-33 37.1727264418539 39.5 36.5 41.0 31.5 41.0 34-35 37.04108091625774 39.0 36.0 41.0 31.0 41.0 36-37 37.027974881447975 40.0 36.0 41.0 31.0 41.0 38-39 36.934667581166586 40.0 36.0 41.0 31.0 41.0 40-41 36.76113070689604 39.5 36.0 41.0 30.5 41.0 42-43 36.50139116143377 39.0 36.0 41.0 30.0 41.0 44-45 36.24342979528089 39.0 35.5 41.0 30.0 41.0 46-47 35.855508877955515 39.0 35.0 41.0 28.5 41.0 48-49 35.50454311265901 39.0 35.0 41.0 27.0 41.0 50-51 34.630204622052986 38.0 34.0 39.5 25.0 40.5 52-53 34.72551697419153 38.0 34.5 40.0 26.0 40.5 54-55 35.05915954437845 39.0 35.0 40.0 26.0 41.0 56-57 34.860023682096966 38.5 34.5 40.0 25.0 41.0 58-59 34.63138306519775 38.0 34.0 40.0 24.0 41.0 60-61 34.338002081889265 38.0 34.0 40.0 23.0 41.0 62-63 34.021104808646555 38.0 34.0 40.0 22.5 41.0 64-65 33.65774193470115 37.0 33.5 40.0 22.0 41.0 66-67 33.27546614018216 37.0 33.0 39.5 20.5 41.0 68-69 32.8000140733773 36.0 32.5 39.0 18.5 41.0 70-71 32.35164395649547 35.5 32.0 39.0 17.5 40.0 72-73 31.962120372669503 35.0 31.5 38.0 14.0 40.0 74-75 31.50613081608603 35.0 31.0 37.5 9.5 39.5 76-77 31.06591355225793 35.0 31.0 37.0 7.5 39.0 78-79 30.592371905980134 35.0 30.5 36.0 4.5 39.0 80-81 30.0914478350859 34.0 30.0 36.0 2.0 37.5 82-83 29.617775565219993 34.0 29.5 35.5 2.0 37.0 84-85 29.176002950556345 34.0 29.0 35.0 2.0 37.0 86-87 28.747752101503018 34.0 29.0 35.0 2.0 36.0 88-89 28.327999065010104 34.0 28.5 35.0 2.0 36.0 90-91 27.900785520921207 34.0 27.5 35.0 2.0 35.5 92-93 27.451466664294145 33.5 26.5 35.0 2.0 35.0 94-95 26.92382218368669 33.0 25.5 35.0 2.0 35.0 96-97 26.29709263436638 33.0 25.0 35.0 2.0 35.0 98-99 25.55409151092264 32.0 22.0 34.5 2.0 35.0 100-101 23.622724702902108 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06404009539155808 1101 2 0.07536876416768479 1101 3 0.05645662251818706 1101 4 0.17250013958685173 1101 5 0.1492985589019682 1101 6 0.062491748686731796 1101 7 0.04960613282288051 1101 8 0.046899211378679695 1101 9 0.25772129507862473 1101 10-11 0.19101662126708163 1101 12-13 0.08714794859302089 1101 14-15 0.2241187323711884 1101 16-17 0.3584707488938008 1101 18-19 0.36939845936733917 1101 20-21 0.2384944247789349 1101 22-23 0.3144890252765009 1101 24-25 0.320171750427356 1101 26-27 0.30329392990350357 1101 28-29 0.19099257064047492 1101 30-31 0.3249216025293933 1101 32-33 0.18889009733552342 1101 34-35 0.31104202170485706 1101 36-37 0.30397240354618305 1101 38-39 0.27920369292834124 1101 40-41 0.12192126715832075 1101 42-43 0.32845548563395965 1101 44-45 0.4650562638043425 1101 46-47 0.47436098179148445 1101 48-49 0.49184702749823117 1101 50-51 0.2985626034200166 1101 52-53 0.28613923205337244 1101 54-55 0.3840417324312213 1101 56-57 0.29603212741168505 1101 58-59 0.3760964696952058 1101 60-61 0.26041333968130687 1101 62-63 0.22003030271849155 1101 64-65 0.32683117855380317 1101 66-67 0.3744224512436105 1101 68-69 0.531276164565682 1101 70-71 0.5100629342088538 1101 72-73 0.44253308944155023 1101 74-75 0.3212926618633496 1101 76-77 0.2789014309243285 1101 78-79 0.3364818044139959 1101 80-81 0.4195991401926342 1101 82-83 0.5451839725445247 1101 84-85 0.3612387926872138 1101 86-87 0.4587046490029749 1101 88-89 0.5691960825501639 1101 90-91 0.5843931045771669 1101 92-93 0.7778628349125114 1101 94-95 0.9150638257901456 1101 96-97 0.9664784304049228 1101 98-99 0.9668118790524467 1101 100-101 1.3654146250534822 1102 1 0.21012987922597404 1102 2 0.16818336963590852 1102 3 0.2845808139281303 1102 4 0.2508117734552684 1102 5 0.19201016629376255 1102 6 0.07176057266332236 1102 7 0.34190404297486054 1102 8 0.3262145499685616 1102 9 0.32224001224611243 1102 10-11 0.3737123426848612 1102 12-13 0.4710504624681775 1102 14-15 0.7579611723926121 1102 16-17 0.6561366941393416 1102 18-19 0.6211204427873511 1102 20-21 0.612971160451977 1102 22-23 0.6461187617338027 1102 24-25 0.5865070247111319 1102 26-27 0.5575382128583755 1102 28-29 0.703378415274841 1102 30-31 0.6999905520693588 1102 32-33 0.7055137060591719 1102 34-35 0.6462300558757477 1102 36-37 0.6734100561127079 1102 38-39 0.9127466662932662 1102 40-41 0.9244668340288484 1102 42-43 0.9623239008275348 1102 44-45 0.8795808980959805 1102 46-47 0.9743193613419052 1102 48-49 0.7877288093749897 1102 50-51 0.7523117534602335 1102 52-53 0.8333944887662952 1102 54-55 0.8166291403898569 1102 56-57 0.8919126055153157 1102 58-59 0.9147693891240323 1102 60-61 0.8973260651087003 1102 62-63 0.8684894434718231 1102 64-65 0.8802843720959146 1102 66-67 0.8823122844208271 1102 68-69 0.863552773070694 1102 70-71 0.9056447479779024 1102 72-73 0.5867389211184211 1102 74-75 0.8201002109102937 1102 76-77 1.1747936429860317 1102 78-79 1.0866126831771332 1102 80-81 1.3261150973727176 1102 82-83 1.1701456115277438 1102 84-85 1.3147617882363356 1102 86-87 1.2539290283568256 1102 88-89 1.2109076959593708 1102 90-91 1.3156699196800723 1102 92-93 1.4592387367724946 1102 94-95 1.3603760593941985 1102 96-97 1.4264897030278938 1102 98-99 1.8428240582991862 1102 100-101 2.2720328782783135 1103 1 0.027600760304192562 1103 2 0.04606384488408821 1103 3 0.12022572532943698 1103 4 0.12714411678656035 1103 5 -0.05219407384132779 1103 6 0.009332625106289072 1103 7 0.13836540516575013 1103 8 0.1572327652981329 1103 9 -0.026257226013989055 1103 10-11 0.17434345423649944 1103 12-13 0.1568973862049745 1103 14-15 0.15762906741699112 1103 16-17 0.12710361579937768 1103 18-19 0.13025406710340803 1103 20-21 -0.046567845510452344 1103 22-23 -0.028260245031006548 1103 24-25 0.09285029464421513 1103 26-27 0.2762758599574653 1103 28-29 0.13709016774365068 1103 30-31 0.001935569786738256 1103 32-33 0.14861661317243602 1103 34-35 0.1717373581732815 1103 36-37 0.2288186060016102 1103 38-39 -0.03519120311378998 1103 40-41 0.1708985787569972 1103 42-43 0.13562352217240203 1103 44-45 -0.08825524535637896 1103 46-47 0.11033047910023441 1103 48-49 -0.030246217558023147 1103 50-51 0.11698266332992802 1103 52-53 0.012800794335916521 1103 54-55 -0.055414570737220004 1103 56-57 -0.010409946353590271 1103 58-59 -0.011712044502765195 1103 60-61 0.021106704727898773 1103 62-63 0.023668428525041918 1103 64-65 0.02641233133473264 1103 66-67 0.13787234356134803 1103 68-69 -0.05537768253083897 1103 70-71 0.1891074659641987 1103 72-73 0.13228963482847078 1103 74-75 0.269278827810183 1103 76-77 0.4565790213992713 1103 78-79 0.44563916543674864 1103 80-81 0.44170757052524934 1103 82-83 0.39459877994726966 1103 84-85 0.5193143226533614 1103 86-87 0.5607358528810273 1103 88-89 0.8279713074254609 1103 90-91 0.9187699594453527 1103 92-93 0.8660602520302056 1103 94-95 0.7490751683923627 1103 96-97 0.8007010348343364 1103 98-99 1.0655827774560187 1103 100-101 1.393794574521145 1104 1 0.33931527786923255 1104 2 0.27782711681451744 1104 3 0.355177743017677 1104 4 0.2161635429700155 1104 5 0.2971007290878802 1104 6 0.2414756817158903 1104 7 0.3814072307604732 1104 8 0.39884349914331807 1104 9 0.5014634416974175 1104 10-11 0.6181369643865438 1104 12-13 0.3874355568649648 1104 14-15 0.6761389710581298 1104 16-17 0.7174779139697804 1104 18-19 0.6748984789135264 1104 20-21 0.6384536570044688 1104 22-23 0.7966941709514828 1104 24-25 0.6449300757095244 1104 26-27 0.7137165186013448 1104 28-29 0.6497104520565102 1104 30-31 0.5049051837270682 1104 32-33 0.638845699553741 1104 34-35 0.6656644171746109 1104 36-37 0.7391739806447433 1104 38-39 0.43736311168040487 1104 40-41 0.4689737012178412 1104 42-43 0.6386851254813948 1104 44-45 0.8275912375286723 1104 46-47 0.8501493914573572 1104 48-49 0.9199885195987818 1104 50-51 0.7737453603268065 1104 52-53 0.8682424582921797 1104 54-55 0.8571922437037927 1104 56-57 0.8498802143978281 1104 58-59 0.8399544414705034 1104 60-61 1.0189804361780759 1104 62-63 0.7163519883821934 1104 64-65 0.8386557877695537 1104 66-67 0.9979256316884815 1104 68-69 1.1056720475789206 1104 70-71 1.0693281090355882 1104 72-73 1.2337548940577996 1104 74-75 1.1392117741311019 1104 76-77 1.1314731514919458 1104 78-79 1.1771448671349383 1104 80-81 1.243028603565861 1104 82-83 1.2440502933366524 1104 84-85 1.4349301750498462 1104 86-87 1.1419440675316501 1104 88-89 1.0065706378742654 1104 90-91 1.2642596878467423 1104 92-93 1.4583850533495983 1104 94-95 1.464877299260376 1104 96-97 1.4731587607914385 1104 98-99 1.636838070951388 1104 100-101 2.2204287820820134 1105 1 0.10579188911135873 1105 2 0.17941298504938885 1105 3 0.23542192941130224 1105 4 0.17374436627817857 1105 5 0.1586242074006634 1105 6 0.17535206608557274 1105 7 0.20075183806218888 1105 8 0.2517391079674667 1105 9 0.2814426948094493 1105 10-11 0.25378168512447274 1105 12-13 0.24122932682825393 1105 14-15 0.3509971757806625 1105 16-17 0.3810271990230589 1105 18-19 0.46181375996669516 1105 20-21 0.3951120647181341 1105 22-23 0.4158305630825936 1105 24-25 0.3973525360814776 1105 26-27 0.27847778013153857 1105 28-29 0.49857497040034104 1105 30-31 0.36423456669373167 1105 32-33 0.37491953757875507 1105 34-35 0.3957589722359529 1105 36-37 0.5247627295626245 1105 38-39 0.44060761151622074 1105 40-41 0.295220380223185 1105 42-43 0.3492115591062728 1105 44-45 0.40002847446111645 1105 46-47 0.437703497550082 1105 48-49 0.48231985784768483 1105 50-51 0.43108363908139324 1105 52-53 0.39233422222543624 1105 54-55 0.4941291671940462 1105 56-57 0.3932367325028423 1105 58-59 0.4965516533275718 1105 60-61 0.6591480531218679 1105 62-63 0.487677624968363 1105 64-65 0.6541916992892993 1105 66-67 0.6039011831974861 1105 68-69 0.7117211087449249 1105 70-71 0.698759957747491 1105 72-73 0.8438936295424391 1105 74-75 0.7829951706504055 1105 76-77 0.6268509985918627 1105 78-79 0.6560804323756884 1105 80-81 0.8501959816965545 1105 82-83 0.9528031665567447 1105 84-85 0.9284463994147565 1105 86-87 1.2915601008460769 1105 88-89 1.0393649003895682 1105 90-91 1.103906766864025 1105 92-93 1.3026680916492488 1105 94-95 1.4035153929348176 1105 96-97 1.4265312495224443 1105 98-99 1.5861384232007048 1105 100-101 1.6460773089037097 1106 1 0.05136220987519735 1106 2 0.09425978336536289 1106 3 -0.012842555398123068 1106 4 0.17632224555216425 1106 5 0.16629392362379036 1106 6 0.20321575371385592 1106 7 0.17335574130765252 1106 8 0.19818331579497084 1106 9 0.22776694018432408 1106 10-11 -0.019727015218073518 1106 12-13 -0.0454253319179756 1106 14-15 -0.03916362644416438 1106 16-17 0.06820496411381072 1106 18-19 0.3368312727334981 1106 20-21 0.33981725480170155 1106 22-23 0.35023649500696763 1106 24-25 0.30398497112662426 1106 26-27 0.23187762111561483 1106 28-29 0.2831123356396006 1106 30-31 0.4520497089324138 1106 32-33 0.5961596341091209 1106 34-35 0.28225303891056086 1106 36-37 0.39760384323449216 1106 38-39 0.2829432366869682 1106 40-41 0.23005318583847867 1106 42-43 0.28617347934612525 1106 44-45 0.2504685702909768 1106 46-47 0.5690939061579527 1106 48-49 0.7169712311988619 1106 50-51 0.5731183073545694 1106 52-53 0.2548432413003425 1106 54-55 0.36929291156369715 1106 56-57 0.391166723466263 1106 58-59 0.5568323479539501 1106 60-61 0.20516022099224074 1106 62-63 0.1865188927525807 1106 64-65 0.5056252068162834 1106 66-67 0.4815911441790419 1106 68-69 0.47009527601287004 1106 70-71 0.40644867532260065 1106 72-73 0.4889812108892464 1106 74-75 0.22266719155363646 1106 76-77 0.3426279875167886 1106 78-79 0.42949894196918237 1106 80-81 0.656287622502699 1106 82-83 0.49312850122571916 1106 84-85 0.36680846536082967 1106 86-87 0.5042101852576124 1106 88-89 0.47423054244262275 1106 90-91 0.5086596442116154 1106 92-93 0.7853317659678289 1106 94-95 0.49956722722201263 1106 96-97 0.73108964759464 1106 98-99 0.911494545313726 1106 100-101 0.9212974443824713 1107 1 0.16038522152328483 1107 2 0.07819800152559253 1107 3 0.16785495164872088 1107 4 0.24303170688092024 1107 5 0.24846952023028024 1107 6 0.24280902064326426 1107 7 0.27328259000259436 1107 8 0.250998951861817 1107 9 0.3371808141218864 1107 10-11 0.36083108099705896 1107 12-13 0.36105323514345855 1107 14-15 0.31669630505156476 1107 16-17 0.398971438171678 1107 18-19 0.3462375657148229 1107 20-21 0.22815321521532894 1107 22-23 0.5446486410772309 1107 24-25 0.46599246741244116 1107 26-27 0.47882776303701746 1107 28-29 0.5900701766620244 1107 30-31 0.5465030087651428 1107 32-33 0.6579596878649951 1107 34-35 0.681672455807032 1107 36-37 0.4256568272268595 1107 38-39 0.4891673986713343 1107 40-41 0.6459075251482957 1107 42-43 0.6113713964537837 1107 44-45 0.5108527574007908 1107 46-47 0.6012479905704211 1107 48-49 0.6645169212283264 1107 50-51 0.6246417349707087 1107 52-53 0.5437825276862256 1107 54-55 0.5591628833960058 1107 56-57 0.5557837424176668 1107 58-59 0.7606237121617951 1107 60-61 0.7588245303425154 1107 62-63 0.8010878169361249 1107 64-65 0.814293193422607 1107 66-67 0.7108264619835509 1107 68-69 0.507029301018207 1107 70-71 0.7792446599755962 1107 72-73 0.6244932651019433 1107 74-75 0.6753797463063584 1107 76-77 0.7961207900387848 1107 78-79 0.7500434318446665 1107 80-81 0.7505385994122911 1107 82-83 0.4563889228253437 1107 84-85 0.7656775245247793 1107 86-87 0.8298777278364966 1107 88-89 0.6617661704756195 1107 90-91 0.7886898302010223 1107 92-93 0.9620276111115338 1107 94-95 1.0502395202052988 1107 96-97 0.9452979348597133 1107 98-99 1.076816002471972 1107 100-101 1.2947013823446554 1108 1 -0.11218568413730523 1108 2 -0.12938522777699646 1108 3 -0.225337954301839 1108 4 -0.01724474187663816 1108 5 -0.09372196035786828 1108 6 0.06650562368641744 1108 7 0.024651935861044194 1108 8 0.12070538745783921 1108 9 0.03400321622471836 1108 10-11 0.1511422882620721 1108 12-13 0.1601307978302131 1108 14-15 0.27303775560729804 1108 16-17 0.15524999955358254 1108 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-2.5155061509144225 2316 88-89 -2.722211552733647 2316 90-91 -3.0031322051329816 2316 92-93 -3.0347040741102056 2316 94-95 -3.0891103477424835 2316 96-97 -3.0511707158893557 2316 98-99 -3.336795451921418 2316 100-101 -3.8374564989706244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4933.0 3 1118.0 4 944.0 5 978.0 6 1088.0 7 1260.0 8 1559.0 9 1949.0 10 2476.0 11 3246.0 12 4267.0 13 5647.0 14 6256.0 15 8369.0 16 10838.0 17 14206.0 18 12007.0 19 7729.0 20 7551.0 21 7816.0 22 8408.0 23 9147.0 24 10250.0 25 11637.0 26 13557.0 27 16015.0 28 18809.0 29 22671.0 30 28058.0 31 35036.0 32 44771.0 33 58588.0 34 80639.0 35 113832.0 36 170172.0 37 246295.0 38 213960.0 39 30292.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1672090543302 19.273806490148562 14.670923530643416 21.88806092487782 2 14.88368282428599 20.624387062548212 40.92114410124003 23.570786011925765 3 19.262265164062743 25.634164502361134 30.2193559480956 24.884214385480526 4 11.967570154483843 16.589593734664163 38.60903704124301 32.83379906960898 5 12.74615492561309 38.3143077081564 34.542440000940935 14.397097365289572 6 30.447986435754625 36.80411919937236 18.027575877429015 14.720318487443995 7 27.325158306064335 33.76453198617182 21.121440645963297 17.788869061800547 8 26.049218126339763 34.38979815948683 20.125780556692423 19.435203157480984 9 26.25024615531732 16.795045612681598 21.128594779319226 35.82611345268186 10-11 24.305831650421144 27.24935753484672 27.557894813631407 20.886916001100733 12-13 25.691468798298672 24.770131757356392 28.270004919524837 21.268394524820096 14-15 22.454412729466025 25.794203778270063 27.526481563499228 24.224901928764687 16-17 20.98995401669144 29.40597734386017 27.753163479819698 21.8509051596287 18-19 20.817625302274923 27.742152197449542 30.796952290806306 20.64327020946923 20-21 21.978054480509797 26.529104027694046 30.631308465742237 20.861533026053923 22-23 21.396605466633876 26.58410949312953 30.70331078225643 21.315974257980162 24-25 21.34907570998494 26.613775241286913 30.833880600098773 21.203268448629377 26-27 21.10573306005073 26.713360772332607 31.00062675654904 21.18027941106762 28-29 20.784342169403093 27.263554869225654 30.869326145508875 21.082776815862378 30-31 20.963827981705286 26.78535968986845 30.84979806273145 21.40101426569482 32-33 20.85577468190389 26.91498944981398 30.772787449513295 21.456448418768836 34-35 21.418871191093636 26.838562632647346 30.61994156931258 21.122624606946435 36-37 21.307422470396904 26.5419418723467 30.86533498314935 21.285300674107045 38-39 21.01117135776179 27.040779325067348 30.718174919485435 21.22987439768543 40-41 21.20117492150309 27.191654005874604 30.487916540058745 21.11925453256356 42-43 20.882092913387105 27.134691105815005 30.215326843490963 21.767889137306923 44-45 21.163938411669367 27.09201782820097 30.10344408427877 21.64059967585089 46-47 21.545980986742748 27.489882922713914 29.53011458929752 21.434021501245816 48-49 21.402546584933237 27.544548621650065 29.46099136158126 21.591913431835433 50-51 21.115764373284744 27.895365631853924 29.511169720420625 21.477700274440704 52-53 21.464917831302543 28.15215791890916 28.821125144257838 21.56179910553046 54-55 21.206037807414706 27.720723243386498 29.050332756957136 22.022906192241653 56-57 21.506816072391473 27.984566251964367 28.763910568852076 21.744707106792088 58-59 21.76320213908076 27.64125833046367 28.926975509956044 21.668564020499524 60-61 21.403294696828826 27.637112596829272 29.603656795053944 21.35593591128796 62-63 21.22399964022175 27.753731787745416 29.582483012834928 21.439785559197905 64-65 21.16695085505663 27.953807131892987 29.24385632504508 21.635385688005304 66-67 21.202312550867084 27.796347738230132 28.916000896111438 22.085338814791346 68-69 21.21849973526551 27.774597723080834 29.169203621130308 21.837698920523344 70-71 21.411077767527566 27.739957321833646 29.008634083948852 21.84033082668994 72-73 21.26660865084034 27.543904854044822 29.2396523587971 21.949834136317744 74-75 21.070750516369458 28.282015109023035 28.82682641640001 21.8204079582075 76-77 21.15076072370852 27.81061804344585 28.866174859491384 22.17244637335425 78-79 21.327098320565657 27.68700581635613 28.819607067788343 22.16628879528987 80-81 21.271881312793685 27.74424459586371 28.814861992657004 22.169012098685602 82-83 21.263154994877162 27.732392249263583 29.12967684198287 21.874775913876384 84-85 21.170143812031597 27.805468908243874 28.869637431638644 22.154749848085885 86-87 21.073108759949978 27.869901757078164 28.802056980165467 22.25493250280639 88-89 21.151089861850096 27.65955378172299 28.572626533434775 22.61672982299214 90-91 20.974199891991447 27.407191036181246 28.935880757907288 22.682728313920016 92-93 21.14667011286991 27.37288715804501 29.01936747316195 22.461075255923127 94-95 20.94656319083869 27.315900921503104 29.116249072805108 22.621286814853107 96-97 20.607957972738834 27.359607663461066 29.343976264060707 22.688458099739396 98-99 20.708854543951848 27.528388051332662 28.83220461760602 22.930552787109466 100-101 20.674126079222738 27.188169292287522 29.388764862415684 22.748939766074052 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1764.0 1 1006.5 2 303.0 3 349.5 4 307.0 5 305.0 6 519.5 7 999.5 8 1642.5 9 2054.5 10 1956.5 11 1800.5 12 1877.5 13 2005.0 14 2138.5 15 2258.5 16 2310.0 17 2422.0 18 2632.5 19 2766.0 20 3032.0 21 3489.5 22 4020.0 23 4812.0 24 5768.0 25 6933.5 26 8788.5 27 10888.0 28 12674.5 29 14678.0 30 17023.5 31 19754.0 32 22491.5 33 24734.5 34 27336.5 35 29888.5 36 33076.5 37 36244.5 38 37528.5 39 37889.5 40 38922.5 41 40372.5 42 41440.5 43 42954.0 44 44961.0 45 46489.5 46 46849.0 47 49517.5 48 55819.0 49 54736.5 50 46790.5 51 41192.0 52 37914.0 53 36326.0 54 34632.5 55 31539.0 56 27415.5 57 23762.0 58 21107.5 59 18214.0 60 15047.0 61 12292.0 62 9746.5 63 7250.5 64 5180.0 65 3840.5 66 2795.5 67 2042.0 68 1512.0 69 1069.0 70 759.0 71 561.0 72 413.5 73 349.0 74 291.5 75 220.0 76 155.5 77 85.0 78 50.5 79 31.5 80 24.5 81 22.0 82 13.0 83 9.5 84 9.5 85 6.5 86 4.0 87 7.5 88 8.0 89 3.5 90 2.5 91 2.5 92 3.0 93 3.0 94 3.5 95 2.5 96 2.0 97 6.0 98 5.5 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3373566476891757 2 0.289879219687846 3 0.2826807680828744 4 0.28809982715628 5 0.28842335307111017 6 0.20584336331070538 7 0.19209351193042254 8 0.20074783015212996 9 0.19411554889811117 10-11 0.21453812227176663 12-13 0.20365956338560162 14-15 0.19836182653025736 16-17 0.19824050431219603 18-19 0.19419643037681872 20-21 0.18772591208021502 22-23 0.19116337492528573 24-25 0.1790311531191538 26-27 0.18161936043779525 28-29 0.1972294858283517 30-31 0.2085124521080544 32-33 0.20321471525271015 34-35 0.21089845572992705 36-37 0.18602740102735654 38-39 0.184692856628682 40-41 0.1821450900493943 42-43 0.18974794904790368 44-45 0.1922552748878376 46-47 0.19803830061542715 48-49 0.20450881891203088 50-51 0.20988743724608272 52-53 0.23524378082089847 54-55 0.20370000412495542 56-57 0.1778179309385406 58-59 0.178101016114017 60-61 0.1764429458005123 62-63 0.184692856628682 64-65 0.17474443474765383 66-67 0.1743400273541161 68-69 0.17159005707805952 70-71 0.18250905670357828 72-73 0.18044657899653585 74-75 0.1844097714532056 76-77 0.18513770476157354 78-79 0.1836818381448377 80-81 0.18408624553837544 82-83 0.17773704945983304 84-85 0.17203490521095102 86-87 0.1731268051735029 88-89 0.17292460147673405 90-91 0.17919291607656887 92-93 0.1753914865773142 94-95 0.17336944960962555 96-97 0.17231799038642745 98-99 0.18044657899653585 100-101 0.16964890158907842 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1236377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.52990518227078 #Duplication Level Percentage of deduplicated Percentage of total 1 80.38387437620723 49.46012168551597 2 11.599154767489313 14.27389786076203 3 3.5456161116190534 6.544842694819559 4 1.4632200527922499 3.6012716443641755 5 0.774498575504211 2.3827411957288946 6 0.47387808800048925 1.7494604293575526 7 0.3234809997494693 1.3932628669995706 8 0.23430294952946906 1.1533310614779688 9 0.1679688587637515 0.9301597157977077 >10 0.951687603247573 10.656843573444089 >50 0.05570091807244581 2.364224975310606 >100 0.023849628287524526 2.541548188229071 >500 0.0013176527319658001 0.5741734875353135 >1k 0.0013176527319658001 1.8344851034258032 >5k 1.3176527319658002E-4 0.5396355172317209 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6656 0.5383471222774283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4968 0.40181918621909013 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3377 0.2731367535953839 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3186 0.25768839116224257 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2508 0.20285074859852617 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1821 0.14728517272644184 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1706 0.13798380267507404 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1610 0.1302191807191496 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 8.088147870754633E-5 0.0 5 0.0 0.0 0.0 8.088147870754633E-5 0.0 6 0.0 0.0 0.0 8.088147870754633E-5 0.0 7 0.0 0.0 0.0 1.6176295741509265E-4 0.0 8 0.0 0.0 0.0 1.6176295741509265E-4 0.0 9 0.0 0.0 0.0 3.235259148301853E-4 0.0 10-11 0.0 0.0 0.0 3.235259148301853E-4 0.0 12-13 0.0 0.0 0.0 3.235259148301853E-4 0.0 14-15 0.0 0.0 0.0 3.235259148301853E-4 0.0 16-17 0.0 0.0 0.0 3.235259148301853E-4 0.0 18-19 0.0 0.0 0.0 3.235259148301853E-4 0.0 20-21 0.0 0.0 0.0 4.044073935377316E-4 0.0 22-23 0.0 0.0 0.0 6.066110903065974E-4 0.0 24-25 0.0 0.0 0.0 0.0012941036593207412 0.0 26-27 0.0 0.0 0.0 0.0016580703135046996 0.0 28-29 0.0 0.0 0.0 0.0031543776695943065 0.0 30-31 0.0 0.0 0.0 0.006308755339188614 0.0 32-33 0.0 0.0 0.0 0.011080762582933846 0.0 34-35 0.0 0.0 0.0 0.016176295741509264 0.0 36-37 0.0 0.0 0.0 0.021029184463962045 0.0 38-39 0.0 0.0 0.0 0.025882073186414823 0.0 40-41 0.0 0.0 0.0 0.0346577136261836 0.0 42-43 0.0 0.0 0.0 0.04484877994333444 0.0 44-45 0.0 0.0 0.0 0.05447367590953245 0.0 46-47 0.0 0.0 0.0 0.06814264581110778 0.0 48-49 0.0 0.0 0.0 0.08071971575013123 0.0 50-51 0.0 0.0 0.0 0.09903937067739048 0.0 52-53 0.0 0.0 0.0 0.14526313575875321 0.0 54-55 0.0 0.0 0.0 0.20224413750821957 0.0 56-57 0.0 0.0 0.0 0.2511774321262851 0.0 58-59 0.0 0.0 0.0 0.3379228180401285 0.0 60-61 0.0 0.0 0.0 0.45152085488487737 0.0 62-63 0.0 0.0 0.0 0.5272259189551407 0.0 64-65 0.0 0.0 0.0 0.5955303277236637 0.0 66-67 0.0 0.0 0.0 0.7712048994764542 0.0 68-69 0.0 0.0 0.0 0.9353538605134195 0.0 70-71 0.0 0.0 0.0 1.0221396871666166 0.0 72-73 0.0 0.0 0.0 1.2003215847593411 0.0 74-75 0.0 0.0 0.0 1.4549769204700507 0.0 76-77 0.0 0.0 0.0 1.7083785932607936 0.0 78-79 0.0 0.0 0.0 1.9873792540624744 0.0 80-81 0.0 0.0 0.0 2.3086000467494947 0.0 82-83 0.0 0.0 0.0 2.591806544443968 0.0 84-85 0.0 0.0 0.0 2.882211493743413 0.0 86-87 0.0 0.0 0.0 3.333813230106998 0.0 88-89 0.0 0.0 0.0 3.929747965224199 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6725 0.0 26.801077 1 TACATGG 6670 0.0 26.443111 2 GTACCGT 90 8.94777E-4 26.388968 6 GTATCAA 10605 0.0 25.784725 1 ACATGGG 6810 0.0 24.712725 3 GAGTACT 5585 0.0 22.58064 12-13 CATGGGA 4675 0.0 22.37385 4 ATCAACG 12225 0.0 22.127272 3 TCAACGC 12230 0.0 22.042269 4 AGTACTT 5910 0.0 21.941692 12-13 CAACGCA 12325 0.0 21.794334 5 TATCAAC 12505 0.0 21.593801 2 GGTATCA 7730 0.0 21.53247 1 AACGCAG 12520 0.0 21.35989 6 AGAGTAC 9495 0.0 20.886055 10-11 ATGGGAT 1920 0.0 20.799847 5 ACCGTCC 115 0.0037233671 20.652237 8 GTACTTT 6080 0.0 20.586 14-15 TGTACCG 140 4.8228365E-4 20.37536 5 CATGGGG 2270 0.0 19.688005 4 >>END_MODULE