FastQCFastQC Report
Thu 26 May 2016
SRR937430_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937430_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1236377
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82850.6701030510920213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68270.5521778551364187No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49940.4039221046654863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45110.36485635044974146No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19090.15440274285270592No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17560.14202787661045135No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.13798380267507404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.10150625577797062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGG700.00838818727.145262
GTCGGGA2155.820766E-1126.5139752
TACATGG67850.024.0846652
GTACATG69650.023.9464451
GAGTACT68400.022.95249212-13
GTATCAA131850.022.1280561
AGTACTT70650.022.08704812-13
ACATGGG69050.021.877393
GTACTTT72500.021.52170814-15
TACCTGG9300.020.9427132
GGACCGA2056.8322515E-720.8529746
CATGGGA44650.020.6401254
GGTATCA101800.019.88461
AGAGTAC114900.019.84429710-11
ATGGGAT17700.019.0553595
ATCAACG152550.018.9331743
TACTTTT82100.018.86052714-15
TATCAAC154050.018.7796572
TCAACGC154100.018.7427374
CAACGCA154250.018.724515
Produced by FastQC (version 0.11.5)