##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937430_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1236377 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15900166373202 33.0 31.0 34.0 31.0 34.0 2 32.364973628593866 34.0 31.0 34.0 31.0 34.0 3 32.44772832234828 34.0 31.0 34.0 31.0 34.0 4 36.071085922821275 37.0 35.0 37.0 35.0 37.0 5 35.94482912574401 37.0 35.0 37.0 35.0 37.0 6 36.044790545278666 37.0 35.0 37.0 35.0 37.0 7 36.0050817833072 37.0 35.0 37.0 35.0 37.0 8 35.983079594654384 37.0 35.0 37.0 35.0 37.0 9 37.67330110476012 39.0 37.0 39.0 35.0 39.0 10-11 37.59681998290166 39.0 37.0 39.0 35.0 39.0 12-13 37.51659323976425 39.0 37.0 39.0 35.0 39.0 14-15 38.685644427225675 40.0 38.0 41.0 34.0 41.0 16-17 38.68658548323044 40.0 38.0 41.0 34.5 41.0 18-19 38.72624288546293 40.0 38.0 41.0 35.0 41.0 20-21 38.65271474639208 40.0 38.0 41.0 34.0 41.0 22-23 38.520416102855364 40.0 38.0 41.0 34.0 41.0 24-25 38.38010170037133 40.0 38.0 41.0 34.0 41.0 26-27 38.15646643378193 40.0 38.0 41.0 33.5 41.0 28-29 38.14241206363431 40.0 38.0 41.0 34.0 41.0 30-31 38.23047379561412 40.0 38.0 41.0 34.0 41.0 32-33 38.15633904545297 40.0 37.0 41.0 34.0 41.0 34-35 38.02398661573291 40.0 37.0 41.0 33.5 41.0 36-37 37.85804653434996 40.0 37.0 41.0 33.0 41.0 38-39 37.707409228738484 40.0 37.0 41.0 33.0 41.0 40-41 37.54176476916021 40.0 37.0 41.0 33.0 41.0 42-43 37.30383936291277 40.0 37.0 41.0 32.5 41.0 44-45 36.95988521300542 40.0 36.0 41.0 31.5 41.0 46-47 36.548459733560236 40.0 36.0 41.0 30.5 41.0 48-49 36.15552012048105 39.0 36.0 41.0 30.0 41.0 50-51 35.90446481938761 39.0 35.5 41.0 29.0 41.0 52-53 35.67899637408331 39.0 35.0 40.5 28.0 41.0 54-55 35.4808941770997 39.0 35.0 40.0 28.0 41.0 56-57 35.22948380631474 38.5 35.0 40.0 27.0 41.0 58-59 34.98279246540497 38.0 35.0 40.0 26.5 41.0 60-61 34.635945993819035 38.0 34.0 40.0 26.0 41.0 62-63 34.28539312847133 37.5 34.0 40.0 25.5 41.0 64-65 33.95163691980682 37.0 34.0 40.0 25.0 41.0 66-67 33.55739713695742 36.5 33.0 39.0 23.5 41.0 68-69 33.12622201804142 36.0 33.0 39.0 22.5 40.0 70-71 32.73246995050862 35.5 32.5 38.5 22.0 40.0 72-73 32.29507059739869 35.0 32.0 38.0 20.5 40.0 74-75 31.85872108588238 35.0 31.5 37.0 19.5 39.0 76-77 30.568440694060143 34.0 30.0 36.0 18.5 38.5 78-79 31.157342380196333 34.5 31.0 36.0 18.0 39.0 80-81 30.977840901278494 35.0 31.0 36.0 18.0 37.5 82-83 30.642691509143248 34.0 31.0 35.5 15.0 37.0 84-85 30.315029315491955 34.0 31.0 35.0 9.5 37.0 86-87 29.994787997512084 34.0 31.0 35.0 7.0 36.0 88-89 29.621491260351817 34.0 30.0 35.0 2.0 36.0 90-91 29.188779797747777 34.0 29.5 35.0 2.0 35.5 92-93 28.7878296830174 34.0 29.0 35.0 2.0 35.0 94-95 28.396506486290185 34.0 29.0 35.0 2.0 35.0 96-97 27.97344054442941 34.0 29.0 35.0 2.0 35.0 98-99 27.353142690295922 33.0 27.0 35.0 2.0 35.0 100-101 25.763488806407757 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10829531751058852 1101 2 0.10404754073491773 1101 3 0.1450366148329465 1101 4 0.06294040032178572 1101 5 0.045333047538740345 1101 6 -0.015671472468163472 1101 7 0.031062365431957062 1101 8 0.07781717904943264 1101 9 0.007475507139396598 1101 10-11 0.1181433312412139 1101 12-13 0.11118336495257353 1101 14-15 0.19715512376649968 1101 16-17 0.07866820006155706 1101 18-19 0.21762501453360272 1101 20-21 0.135551662235315 1101 22-23 0.08785845304146278 1101 24-25 0.14579780615685678 1101 26-27 0.047088950467632174 1101 28-29 0.07945061545777321 1101 30-31 0.0786340816308666 1101 32-33 -0.03048164208142623 1101 34-35 -0.037090511106903534 1101 36-37 -0.04251875043983944 1101 38-39 -0.008390565053666421 1101 40-41 0.04305098723092016 1101 42-43 -0.12173268256537995 1101 44-45 -0.19309682975406162 1101 46-47 -0.2608786524497404 1101 48-49 -0.3756740852350191 1101 50-51 -0.4346780707242459 1101 52-53 -0.33579168580783403 1101 54-55 -0.3963537856254362 1101 56-57 -0.3393889050736121 1101 58-59 -0.38056126499611054 1101 60-61 -0.39803401173541175 1101 62-63 -0.384197786797678 1101 64-65 -0.41644673363163065 1101 66-67 -0.5229008281855911 1101 68-69 -0.42285563488852773 1101 70-71 -0.5464045214156243 1101 72-73 -0.5008132936688412 1101 74-75 -0.343458603355451 1101 76-77 -0.3089883432080711 1101 78-79 -0.33775014277448534 1101 80-81 -0.34695366138035055 1101 82-83 -0.3603547879284079 1101 84-85 -0.2883175867089882 1101 86-87 -0.3827768605483435 1101 88-89 -0.43601842616673636 1101 90-91 -0.26484545767122825 1101 92-93 -0.2478552870615296 1101 94-95 -0.5314590601747327 1101 96-97 -0.2567123951377823 1101 98-99 -0.3588133677156691 1101 100-101 -0.1601005951829393 1102 1 0.16638141868739353 1102 2 0.11896771973293596 1102 3 0.019068061840592065 1102 4 0.05250319504972367 1102 5 0.00912927381759232 1102 6 0.10711487121044172 1102 7 0.1284642464756942 1102 8 0.13056182204626765 1102 9 0.23268125256666394 1102 10-11 0.17760541451018952 1102 12-13 0.15687258956861427 1102 14-15 0.1794208006353557 1102 16-17 0.20570434265822968 1102 18-19 0.20069251187089066 1102 20-21 0.24845891582720725 1102 22-23 0.21789026510557363 1102 24-25 0.15942875412026325 1102 26-27 0.19571557592170308 1102 28-29 0.16381476530743555 1102 30-31 0.1702351751956357 1102 32-33 0.1618589917127622 1102 34-35 0.28949011321990525 1102 36-37 0.2583775434346336 1102 38-39 0.23375470335310666 1102 40-41 0.18709635452386664 1102 42-43 0.30533124042776194 1102 44-45 0.45205724614158527 1102 46-47 0.4213050872930779 1102 48-49 0.3342955669383656 1102 50-51 0.29022479817835745 1102 52-53 0.41648490263913374 1102 54-55 0.3770384113927321 1102 56-57 0.3199757963723684 1102 58-59 0.22286605366409873 1102 60-61 0.2609139837635652 1102 62-63 0.4401152752037447 1102 64-65 0.4004573868931658 1102 66-67 0.519015979054565 1102 68-69 0.43629374341227134 1102 70-71 0.3806578972239194 1102 72-73 0.5038572228058413 1102 74-75 0.3974596139654736 1102 76-77 0.37703801707859697 1102 78-79 0.44657519475231666 1102 80-81 0.6298823018973785 1102 82-83 0.5315182927671209 1102 84-85 0.5196040384742915 1102 86-87 0.5963998769299188 1102 88-89 0.7069342789619064 1102 90-91 0.5705236003827814 1102 92-93 0.38715693831871434 1102 94-95 0.19293333140650404 1102 96-97 0.5088937691015261 1102 98-99 0.5740734066092301 1102 100-101 0.800803762026824 1103 1 0.1832557025878856 1103 2 0.16793919139043822 1103 3 0.13589774255017772 1103 4 0.0973134721912956 1103 5 0.056772089576909934 1103 6 0.15127439015676458 1103 7 0.0771446174137651 1103 8 0.1059285888931143 1103 9 0.09854187585495566 1103 10-11 0.09173453720790548 1103 12-13 0.13363999100794643 1103 14-15 0.08186371032097384 1103 16-17 0.0396138005406641 1103 18-19 0.07326072348353563 1103 20-21 -0.012130212086013614 1103 22-23 5.146082999871737E-4 1103 24-25 0.022896970806812078 1103 26-27 0.08413915991788912 1103 28-29 0.06618481131480536 1103 30-31 0.20530059910885257 1103 32-33 0.251040593958507 1103 34-35 0.32359709951916926 1103 36-37 0.34319499484607263 1103 38-39 0.30999459536146645 1103 40-41 0.2872193082934871 1103 42-43 0.1412089026069765 1103 44-45 0.111082371425816 1103 46-47 0.391519182609386 1103 48-49 0.25346496820543507 1103 50-51 0.07967148261549539 1103 52-53 0.25063891108541725 1103 54-55 0.4046050713335205 1103 56-57 0.2020519805103902 1103 58-59 0.18405714765764714 1103 60-61 0.0796191224523497 1103 62-63 0.41120432464838785 1103 64-65 0.3518278140336122 1103 66-67 0.45889282468858283 1103 68-69 0.27272806049376896 1103 70-71 0.3983850106540814 1103 72-73 0.4136800136204144 1103 74-75 0.4179649869980082 1103 76-77 0.13265943510607414 1103 78-79 0.5149286998197375 1103 80-81 0.4720002808772392 1103 82-83 0.4467144111095358 1103 84-85 0.28078581063694585 1103 86-87 0.45484138480096803 1103 88-89 0.533948826918003 1103 90-91 0.6490833303092778 1103 92-93 0.6126788723508341 1103 94-95 0.5843257415481844 1103 96-97 0.4621085027862577 1103 98-99 0.451835294008589 1103 100-101 0.6250807596420884 1104 1 0.2279263970747678 1104 2 0.21930474071481143 1104 3 0.20052881590184768 1104 4 0.07461115307361155 1104 5 0.12749351620060878 1104 6 0.19210847090371885 1104 7 0.12093352809537805 1104 8 0.19419633052126528 1104 9 0.21558717733368127 1104 10-11 0.39917877686649206 1104 12-13 0.23544856770244138 1104 14-15 0.40571523947996013 1104 16-17 0.29009099539057104 1104 18-19 0.29525998099791906 1104 20-21 0.2671679090311301 1104 22-23 0.24953869965857223 1104 24-25 0.15119060087156555 1104 26-27 0.2516987805274482 1104 28-29 0.28278100755440505 1104 30-31 0.31163866660933337 1104 32-33 0.22417509884215292 1104 34-35 0.33979807000701356 1104 36-37 0.46155048776124374 1104 38-39 0.42769729229262765 1104 40-41 0.33304808322507284 1104 42-43 0.537872316792388 1104 44-45 0.4733847996166176 1104 46-47 0.31670521468082313 1104 48-49 0.3016079328256609 1104 50-51 0.33274540592921653 1104 52-53 0.46381934494670674 1104 54-55 0.5119550218892357 1104 56-57 0.49749587849417765 1104 58-59 0.4129533080871042 1104 60-61 0.2291882508202221 1104 62-63 0.23236286503993853 1104 64-65 0.46958471898560106 1104 66-67 0.6157944913388249 1104 68-69 0.5803828900998838 1104 70-71 0.44977224318677855 1104 72-73 0.44145423228570735 1104 74-75 0.35149149800551527 1104 76-77 0.5411898063817375 1104 78-79 0.554608971056961 1104 80-81 0.552514961394877 1104 82-83 0.514167586878223 1104 84-85 0.4588029797955393 1104 86-87 0.41911632788254494 1104 88-89 0.5248780181014787 1104 90-91 0.7564710622128352 1104 92-93 0.7277030359867815 1104 94-95 0.5728852208883559 1104 96-97 0.629122757853068 1104 98-99 0.9502488059327128 1104 100-101 1.2020850601450732 1105 1 -0.00737540297896544 1105 2 0.004118466497672557 1105 3 -0.0326251588436719 1105 4 -0.06086378647682267 1105 5 -0.15368875789712888 1105 6 -0.012240166369032579 1105 7 -0.0030679346348563286 1105 8 -7.639668010455125E-4 1105 9 -0.026768564628760316 1105 10-11 2.4074886565728093E-4 1105 12-13 -0.12661522524659574 1105 14-15 0.10063859540102982 1105 16-17 0.012892422298840245 1105 18-19 -0.02792578574138105 1105 20-21 0.04512434056640302 1105 22-23 0.07919667452349444 1105 24-25 -0.036615751556865916 1105 26-27 0.07956229884143085 1105 28-29 -0.03171644013059449 1105 30-31 -0.20689173728332122 1105 32-33 -0.03896671136114094 1105 34-35 -0.08501199895027156 1105 36-37 -0.03832674738010411 1105 38-39 -0.05379394625180822 1105 40-41 -0.09151309770570748 1105 42-43 -0.20344981222638125 1105 44-45 -0.049699098410194154 1105 46-47 0.08665879538472154 1105 48-49 0.11112053021662405 1105 50-51 -0.011713010739939023 1105 52-53 -0.07968008787218395 1105 54-55 -0.053929407496717374 1105 56-57 0.0023340826196758258 1105 58-59 -0.04033970065942327 1105 60-61 0.021388391122918904 1105 62-63 0.01582169963201352 1105 64-65 0.11054862835602819 1105 66-67 0.02713027748169594 1105 68-69 -0.08422687437069243 1105 70-71 -0.18243916610902744 1105 72-73 0.02875161429711426 1105 74-75 0.04026429719355562 1105 76-77 -0.12213708397320033 1105 78-79 -0.1364601653351727 1105 80-81 -0.2572767488054275 1105 82-83 -0.24280986020974993 1105 84-85 -0.21485556225049507 1105 86-87 -0.021899075562334502 1105 88-89 0.04214345886090598 1105 90-91 -0.12698441407004069 1105 92-93 0.020459536180815974 1105 94-95 -0.010860935713331799 1105 96-97 0.05513099690497825 1105 98-99 0.052366033312633675 1105 100-101 -0.02938901370787761 1106 1 -0.046918354790840056 1106 2 0.07898466895926504 1106 3 -0.010363805316949026 1106 4 0.10046819954098396 1106 5 0.025441453026637362 1106 6 0.07590246460895145 1106 7 0.04337854985233491 1106 8 0.03655311234965808 1106 9 0.019861803909904552 1106 10-11 -0.015272935610326499 1106 12-13 -0.11386155281940091 1106 14-15 0.033646570715035296 1106 16-17 0.22570493762250976 1106 18-19 0.12406401482322593 1106 20-21 -0.11024323489611021 1106 22-23 0.010578589439468544 1106 24-25 0.06421487897279121 1106 26-27 0.10659500568471714 1106 28-29 0.046469307279267014 1106 30-31 -0.02668711811595159 1106 32-33 0.04620881910057051 1106 34-35 0.09474560684434863 1106 36-37 0.1409308689221831 1106 38-39 0.07184351845997128 1106 40-41 0.02164476937939952 1106 42-43 -0.09902643758627505 1106 44-45 -0.08302895837810809 1106 46-47 -0.3167136381193032 1106 48-49 -0.4571770712348311 1106 50-51 -0.35867988709279786 1106 52-53 -0.3892353161335862 1106 54-55 -0.3947405463169176 1106 56-57 -0.4592928183911553 1106 58-59 -0.2024470644276093 1106 60-61 -0.15322396378380887 1106 62-63 -0.2388634033080237 1106 64-65 -0.25733330757526574 1106 66-67 -0.24859641477696215 1106 68-69 -0.16488936482623728 1106 70-71 -0.30878852479150254 1106 72-73 -0.4819075312199672 1106 74-75 -0.4727231706705197 1106 76-77 -0.43459592897120203 1106 78-79 -0.47192159171500236 1106 80-81 -0.49595518288387197 1106 82-83 -0.564007575844446 1106 84-85 -0.5376924728583283 1106 86-87 -0.5430851452071046 1106 88-89 -0.46857873261668104 1106 90-91 -0.28524290951321873 1106 92-93 -0.3035389086854927 1106 94-95 -0.39788651486053084 1106 96-97 -0.3127544415796777 1106 98-99 -0.5524820259225507 1106 100-101 -0.240874936945918 1107 1 -0.010933426125667722 1107 2 0.06200431151225416 1107 3 0.09073267662708417 1107 4 0.05035512514817242 1107 5 0.02978721158007147 1107 6 0.062229176255954144 1107 7 0.030420037373787068 1107 8 -0.047145567284054835 1107 9 0.023768457725040548 1107 10-11 0.010807522948923065 1107 12-13 0.0810640307419348 1107 14-15 -0.011524606292418582 1107 16-17 -0.08438718576395132 1107 18-19 0.10965232164125638 1107 20-21 0.23982858728025747 1107 22-23 0.1822252223626748 1107 24-25 0.27439984445975085 1107 26-27 0.32817109633326425 1107 28-29 0.17888252255971793 1107 30-31 0.11671651463890242 1107 32-33 -0.021337993304236136 1107 34-35 -0.0964357719642166 1107 36-37 0.009915700117915094 1107 38-39 -0.060287133905930546 1107 40-41 -0.11351137370573383 1107 42-43 -0.2713750473668881 1107 44-45 -0.23929954141271992 1107 46-47 -0.4464454593308105 1107 48-49 -0.597823992628193 1107 50-51 -0.6986065191889921 1107 52-53 -0.7957370198796596 1107 54-55 -0.7190528698996985 1107 56-57 -0.8965200393405937 1107 58-59 -0.533863334734967 1107 60-61 -0.5913958506444388 1107 62-63 -0.8902162101816913 1107 64-65 -0.7050252017608685 1107 66-67 -0.6829044753025286 1107 68-69 -0.7490739148374175 1107 70-71 -0.8104898095174491 1107 72-73 -0.6703440131443088 1107 74-75 -0.5316454146729477 1107 76-77 -0.41572673197168797 1107 78-79 -0.5375583969622078 1107 80-81 -0.6247792584256402 1107 82-83 -0.5978270092811897 1107 84-85 -0.5958236391539877 1107 86-87 -0.3915088478052091 1107 88-89 -0.18966452245878784 1107 90-91 -0.26329407210700495 1107 92-93 -0.37163952471202677 1107 94-95 -0.6366797447712429 1107 96-97 -0.14261070861837055 1107 98-99 -0.2988325831228309 1107 100-101 -0.16820556410759835 1108 1 0.017849668360611304 1108 2 -0.02906448434836051 1108 3 -0.04925598825835209 1108 4 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-0.23655615209986536 2316 52-53 -0.24435390437454174 2316 54-55 -0.4013379885683719 2316 56-57 -0.009819686428578223 2316 58-59 0.06604741187278051 2316 60-61 0.07877184943563265 2316 62-63 -0.015246303108646941 2316 64-65 0.11199509023414578 2316 66-67 0.07934802709153388 2316 68-69 0.18135034639237801 2316 70-71 0.29531513838389145 2316 72-73 0.24809835912347467 2316 74-75 0.15040475625487915 2316 76-77 0.26202304823848976 2316 78-79 0.22617512325934896 2316 80-81 0.2629919568649548 2316 82-83 0.37947088662135897 2316 84-85 0.04908963080141504 2316 86-87 0.1636873759114046 2316 88-89 0.25764566027438107 2316 90-91 0.19463289525710437 2316 92-93 0.022730735094700805 2316 94-95 0.1840965903293288 2316 96-97 0.15042618932247365 2316 98-99 0.14991180621812106 2316 100-101 -0.2748624762077192 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 11.0 5 20.0 6 52.0 7 106.0 8 281.0 9 603.0 10 1027.0 11 1811.0 12 2839.0 13 4176.0 14 5322.0 15 7608.0 16 10878.0 17 15722.0 18 13939.0 19 7419.0 20 6012.0 21 5981.0 22 6018.0 23 6386.0 24 7147.0 25 8427.0 26 10326.0 27 12700.0 28 15932.0 29 20477.0 30 25689.0 31 32432.0 32 41992.0 33 55882.0 34 77073.0 35 112324.0 36 175300.0 37 277225.0 38 241704.0 39 25518.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90720738508547 19.99760476716071 15.151142428960307 20.944045418793515 2 15.527383637838621 21.749838439246282 39.62205702629538 23.10072089661972 3 19.988078070038505 25.85457348365426 30.45187673339119 23.705471712916044 4 12.8069351015103 17.412083854681864 37.717540847168785 32.06344019663906 5 12.840277755307412 38.850716229465235 33.993072295978955 14.315933719248402 6 30.62197048311316 37.48880802538384 17.23786514954581 14.651356341957186 7 27.591422357420107 34.20882141935672 20.564035079914945 17.63572114330823 8 26.60474919866675 34.709963061428674 19.5303697820325 19.154917957872073 9 26.539235200913637 17.039462882276197 20.69546748281471 35.72583443399546 10-11 24.69513748638158 27.64791807029733 27.006285299710363 20.650659143610728 12-13 26.250991357746585 25.03915888375848 27.90162981033471 20.808219948160232 14-15 22.760816482351256 26.18105157245727 27.35941383574751 23.698718109443963 16-17 20.984254802540004 29.634528950312085 27.757027185073806 21.62418906207411 18-19 20.927718648923427 27.703645409126825 31.082307419177162 20.286328522772585 20-21 22.060059350829075 26.329226441449492 31.215155247954307 20.395558959767126 22-23 21.54470443265086 26.455331155703497 31.19909702639079 20.800867385254858 24-25 21.497702151614316 26.562795723624085 31.409832299935132 20.52966982482647 26-27 21.35173806101675 26.56499095320173 31.550516884795478 20.532754100986047 28-29 20.875028713598436 27.18607544154235 31.455463995574064 20.483431849285154 30-31 21.15279157270836 26.671635537754252 31.306840056811748 20.868732832725637 32-33 21.076257484569837 26.859525856595525 31.289000038014297 20.77521662082035 34-35 21.548686201700615 26.68935122539484 31.245930650602528 20.516031922302016 36-37 21.53298397813605 26.44566000713377 31.36771831801568 20.6536376967145 38-39 21.144615514026327 26.98836878721323 31.22152201017251 20.645493688587937 40-41 21.307780717370186 27.14087208027972 30.931059053994048 20.620288148356046 42-43 21.085229807399475 27.222715869230147 30.6295542980926 21.06250002527778 44-45 21.42881985025603 27.028568147094294 30.41349847174446 21.129113530905215 46-47 21.867399668547698 27.593282631430384 29.75839893495269 20.78091876506923 48-49 21.854458631954493 27.67481116196759 29.39228892158298 21.07844128449494 50-51 21.51197470996901 28.049101547950805 29.532064059774672 20.906859682305512 52-53 21.864370382922 28.30606745771609 28.764221520259436 21.065340639102473 54-55 21.538786931003624 28.09847971067066 28.886103868845776 21.476629489479944 56-57 21.89320086025541 28.21586781378172 28.71320802635442 21.177723299608452 58-59 22.229465607982032 27.828890378905463 28.716766811417553 21.22487720169495 60-61 21.70482789634553 28.08451629236066 29.417645265157795 20.79301054613601 62-63 21.565913956665323 27.99494005469206 29.4954128069351 20.943733181707522 64-65 21.579057196955297 28.25469092356134 29.093553179976656 21.072698699506702 66-67 21.596689359313544 28.015403068805067 28.837280214691795 21.550627357189594 68-69 21.539384831649247 28.148938390151223 29.006524708887337 21.305152069312193 70-71 21.81357304446783 28.182180677900025 28.706777948797168 21.297468328834977 72-73 21.836422062202708 27.748413307591456 29.02290320832562 21.392261421880217 74-75 21.50606166242174 28.75453846197398 28.416049473582895 21.32335040202139 76-77 21.651607883355968 28.082494255392977 28.67733709054762 21.588560770703435 78-79 21.769452197832862 28.05952391544003 28.478651738102535 21.69237214862457 80-81 21.729618069569394 28.149625882720237 28.549786998625827 21.570969049084542 82-83 21.706408535601472 28.15955036277439 28.753747347587645 21.3802937540365 84-85 21.41160941331763 28.246868266611454 28.577471578225396 21.764050741845523 86-87 21.300663147243924 28.282433270758027 28.463809986759703 21.953093595238347 88-89 21.49057285924924 28.28312076332704 28.14942367902347 22.076882698400247 90-91 21.48814677877991 27.881529665508513 28.375823367854085 22.25450018785749 92-93 21.33756944306831 27.98105235998251 28.562843423953243 22.118534772995933 94-95 21.263053259644916 27.9375142048097 28.59172404533569 22.2077084902097 96-97 21.03136826388715 27.868400981254098 28.887750257405305 22.212480497453445 98-99 21.090737767568637 28.120983690091517 28.188156410121607 22.600122132218235 100-101 20.605011496391327 28.44217325274063 28.34211319243766 22.610702058430384 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 473.0 1 348.0 2 267.0 3 350.5 4 362.5 5 370.0 6 584.5 7 1202.0 8 1914.0 9 2301.0 10 2276.0 11 2136.5 12 2165.0 13 2324.5 14 2526.0 15 2666.5 16 2777.0 17 2905.0 18 3096.5 19 3247.0 20 3511.5 21 3912.0 22 4405.0 23 5083.0 24 5877.0 25 7078.5 26 8895.5 27 10904.0 28 12849.0 29 14894.0 30 17200.5 31 19775.5 32 22305.5 33 24521.5 34 27244.5 35 29644.0 36 32278.0 37 35505.0 38 37182.0 39 37928.0 40 38811.5 41 40093.0 42 40851.5 43 42165.0 44 44920.0 45 46177.0 46 46058.0 47 49322.0 48 56185.5 49 54382.5 50 46072.5 51 41065.0 52 37861.0 53 36322.0 54 34796.5 55 31570.5 56 27439.0 57 23859.5 58 21309.0 59 18478.0 60 15058.5 61 12183.0 62 9741.5 63 7346.5 64 5209.5 65 3772.0 66 2750.5 67 2087.5 68 1508.0 69 1023.5 70 738.0 71 538.5 72 425.5 73 375.5 74 260.0 75 205.0 76 161.0 77 77.0 78 48.5 79 30.5 80 19.5 81 15.0 82 8.5 83 2.0 84 1.0 85 1.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04763919095874478 2 0.0 3 0.0 4 0.0 5 0.014639547646065884 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005459499812759377 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014154258773820606 24-25 2.4264443612263896E-4 26-27 0.002264681403811297 28-29 0.0023455628825188434 30-31 0.0010514592231981022 32-33 0.0 34-35 0.0 36-37 3.235259148301853E-4 38-39 4.448481328915048E-4 40-41 0.0 42-43 0.009180047833306507 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.0440739353773164E-5 52-53 2.022036967688658E-4 54-55 4.0440739353773164E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.022036967688658E-4 84-85 8.088147870754633E-5 86-87 0.0 88-89 0.0 90-91 0.005459499812759377 92-93 0.0018198332709197921 94-95 0.0 96-97 0.0 98-99 9.705777444905558E-4 100-101 0.009665336705551785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1236377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.14732066271233 #Duplication Level Percentage of deduplicated Percentage of total 1 79.24803540370846 46.87308961913123 2 12.00162238745726 14.197276156474434 3 3.785160223369993 6.716462564742265 4 1.6489040866877736 3.901130350295143 5 0.8775354767284683 2.5951936117482424 6 0.5222309457272136 1.8533136724151416 7 0.3563678127339263 1.4754740905550072 8 0.24642805543099647 1.1660447371892637 9 0.1803396117045339 0.9599944357509386 >10 1.0368363559254914 11.052876777379197 >50 0.06439179605233569 2.6279057287279417 >100 0.028698514764713624 2.9818603240709436 >500 0.0017936514486475276 0.6899093328134953 >1k 0.0013797318835750212 1.6762169027666423 >5k 2.7594637671500425E-4 1.2332516959400925 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8285 0.6701030510920213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6827 0.5521778551364187 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4994 0.4039221046654863 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4511 0.36485635044974146 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1909 0.15440274285270592 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1756 0.14202787661045135 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1706 0.13798380267507404 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.10150625577797062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 8.088147870754633E-5 0.0 9 0.0 0.0 0.0 1.6176295741509265E-4 0.0 10-11 0.0 0.0 0.0 1.6176295741509265E-4 0.0 12-13 0.0 0.0 0.0 3.235259148301853E-4 0.0 14-15 0.0 0.0 0.0 3.235259148301853E-4 0.0 16-17 0.0 0.0 0.0 3.235259148301853E-4 0.0 18-19 0.0 0.0 0.0 3.235259148301853E-4 0.0 20-21 0.0 0.0 0.0 4.044073935377316E-4 0.0 22-23 0.0 0.0 0.0 6.066110903065974E-4 0.0 24-25 0.0 0.0 0.0 0.0013749851380282876 0.0 26-27 0.0 0.0 0.0 0.001738951792212246 0.0 28-29 8.088147870754633E-5 0.0 0.0 0.0031948184089480797 0.0 30-31 8.088147870754633E-5 0.0 0.0 0.006389636817896159 0.0 32-33 8.088147870754633E-5 0.0 0.0 0.011242525540348939 0.0 34-35 8.088147870754633E-5 0.0 0.0 0.016418940177631902 0.0 36-37 8.088147870754633E-5 0.0 0.0 0.021554914075561094 0.0 38-39 8.088147870754633E-5 0.0 0.0 0.026488684276721422 0.0 40-41 8.088147870754633E-5 0.0 0.0 0.035304765455843976 0.0 42-43 8.088147870754633E-5 0.0 0.0 0.04569803546976367 0.0 44-45 8.088147870754633E-5 4.0440739353773164E-5 0.0 0.0556060166114381 0.0 46-47 8.088147870754633E-5 8.088147870754633E-5 0.0 0.0690323420768908 0.0 48-49 8.088147870754633E-5 8.088147870754633E-5 0.0 0.08148808979785292 0.0 50-51 8.088147870754633E-5 8.088147870754633E-5 0.0 0.09972686324640462 0.0 52-53 8.088147870754633E-5 8.088147870754633E-5 0.0 0.14554622093422961 0.0 54-55 8.088147870754633E-5 8.088147870754633E-5 0.0 0.20289118933787995 0.0 56-57 8.088147870754633E-5 8.088147870754633E-5 0.0 0.25243109504625205 0.0 58-59 8.088147870754633E-5 8.088147870754633E-5 0.0 0.3393786846568644 0.0 60-61 8.088147870754633E-5 8.088147870754633E-5 0.0 0.453542891852566 0.0 62-63 8.088147870754633E-5 8.088147870754633E-5 0.0 0.5301780929279661 0.0 64-65 8.088147870754633E-5 8.088147870754633E-5 0.0 0.5986847053932578 0.0 66-67 8.088147870754633E-5 8.088147870754633E-5 0.0 0.7766643992892135 0.0 68-69 8.088147870754633E-5 8.088147870754633E-5 0.0 0.9419457010280845 0.0 70-71 8.088147870754633E-5 8.088147870754633E-5 0.0 1.029014612856758 0.0 72-73 8.088147870754633E-5 8.088147870754633E-5 0.0 1.2070751882314212 0.0 74-75 8.088147870754633E-5 8.088147870754633E-5 0.0 1.4636312386917583 0.0 76-77 8.088147870754633E-5 8.088147870754633E-5 0.0 1.7185292188385906 0.0 78-79 8.088147870754633E-5 8.088147870754633E-5 0.0 2.00104822396405 0.0 80-81 8.088147870754633E-5 8.088147870754633E-5 0.0 2.326191768368386 0.0 82-83 8.088147870754633E-5 8.088147870754633E-5 0.0 2.611662947466671 0.0 84-85 8.088147870754633E-5 8.088147870754633E-5 0.0 2.9033620004254366 0.0 86-87 8.088147870754633E-5 8.088147870754633E-5 0.0 3.3607063217772573 0.0 88-89 8.088147870754633E-5 8.088147870754633E-5 0.0 3.9639203899781377 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTAGG 70 0.008388187 27.14526 2 GTCGGGA 215 5.820766E-11 26.513975 2 TACATGG 6785 0.0 24.084665 2 GTACATG 6965 0.0 23.946445 1 GAGTACT 6840 0.0 22.952492 12-13 GTATCAA 13185 0.0 22.128056 1 AGTACTT 7065 0.0 22.087048 12-13 ACATGGG 6905 0.0 21.87739 3 GTACTTT 7250 0.0 21.521708 14-15 TACCTGG 930 0.0 20.942713 2 GGACCGA 205 6.8322515E-7 20.852974 6 CATGGGA 4465 0.0 20.640125 4 GGTATCA 10180 0.0 19.8846 1 AGAGTAC 11490 0.0 19.844297 10-11 ATGGGAT 1770 0.0 19.055359 5 ATCAACG 15255 0.0 18.933174 3 TACTTTT 8210 0.0 18.860527 14-15 TATCAAC 15405 0.0 18.779657 2 TCAACGC 15410 0.0 18.742737 4 CAACGCA 15425 0.0 18.72451 5 >>END_MODULE