FastQCFastQC Report
Thu 26 May 2016
SRR937429_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937429_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349594
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70010.5187486014312452No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51980.3851528681959167No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38270.2835667615594023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34770.2576330363057334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26950.1996896844532504No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20200.14967464289260324No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18980.14063488723275297No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17090.12663067559577176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG74550.026.0871221
TACATGG73100.026.0143152
GAGTACT56350.024.7838112-13
GTATCAA117750.024.592741
ACATGGG74450.024.010043
GTATAGG3400.023.7751
TATTCCG1401.7689012E-523.7688125
GTACTTT62000.022.21799514-15
GGTATCA87450.021.6951981
CATGGGA48900.021.6811314
TATTACG1100.00286319221.609622
ATCAACG135650.021.2033943
CCGTTGC1353.7951674E-421.1121359
TCAACGC136900.021.010574
TATCAAC137850.020.8994872
CAACGCA137750.020.8440845
AGTACTT59850.020.51686912-13
AACGCAG140400.020.4369836
GTGTACG1656.272766E-520.1727281
AGAGTAC102100.020.16870510-11
Produced by FastQC (version 0.11.5)