FastQCFastQC Report
Thu 26 May 2016
SRR937429_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937429_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349594
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90290.6690160151867894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71910.5328269094260941No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51980.3851528681959167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48370.3584040830057039No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19940.147748137588045No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18110.13418850409826955No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18100.13411440774040193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.10588369539283667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG74500.024.2958052
GTACATG75150.024.2897131
TAACGCA1000.00165226723.753774
GTATCAA138850.023.6565991
ACATGGG73500.023.3982723
GAGTACT78050.022.85173212-13
GGTATCA105400.021.648151
GTACTTT83050.021.24639114-15
AGTACTT80200.020.9657812-13
CATGGGA50800.020.8546894
CCCTATA2309.008363E-820.6554532
ATCAACG163500.020.0781123
TCAACGC163750.020.076474
CAACGCA165150.019.9062795
TATCAAC165400.019.9049132
AACGCAG167350.019.6969836
AGAGTAC125800.019.67157710-11
GTAAGGT4350.019.6582934
TACCTGG10050.019.3811872
ACTTTTT95700.018.58685316-17
Produced by FastQC (version 0.11.5)