##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937429_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1349594 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09168016455319 31.0 31.0 34.0 31.0 34.0 2 32.2981993103111 33.0 31.0 34.0 31.0 34.0 3 32.37818484670205 34.0 31.0 34.0 30.0 34.0 4 36.009041237587006 37.0 35.0 37.0 35.0 37.0 5 35.91474473063751 37.0 35.0 37.0 35.0 37.0 6 36.004452450144264 37.0 35.0 37.0 35.0 37.0 7 35.96055776774348 37.0 35.0 37.0 35.0 37.0 8 35.93997157663712 37.0 35.0 37.0 35.0 37.0 9 37.60999456132733 39.0 37.0 39.0 35.0 39.0 10-11 37.540573683641156 39.0 37.0 39.0 35.0 39.0 12-13 37.46736314773184 39.0 37.0 39.0 35.0 39.0 14-15 38.62184442135931 40.0 38.0 41.0 34.0 41.0 16-17 38.61682513407736 40.0 38.0 41.0 34.0 41.0 18-19 38.6535283944653 40.0 38.0 41.0 34.0 41.0 20-21 38.55726944547767 40.0 38.0 41.0 34.0 41.0 22-23 38.43083845956636 40.0 38.0 41.0 34.0 41.0 24-25 38.29438890510776 40.0 38.0 41.0 34.0 41.0 26-27 38.227079403139015 40.0 38.0 41.0 34.0 41.0 28-29 38.13299258888229 40.0 38.0 41.0 34.0 41.0 30-31 38.17877746937227 40.0 37.5 41.0 34.0 41.0 32-33 38.07308790643705 40.0 37.0 41.0 34.0 41.0 34-35 37.94377531316826 40.0 37.0 41.0 33.0 41.0 36-37 37.77263236202888 40.0 37.0 41.0 33.0 41.0 38-39 37.63565301861152 40.0 37.0 41.0 33.0 41.0 40-41 37.46185149015185 40.0 37.0 41.0 33.0 41.0 42-43 37.2254922591535 40.0 36.0 41.0 32.5 41.0 44-45 36.87912438851981 40.0 36.0 41.0 31.0 41.0 46-47 36.44927585629456 39.5 36.0 41.0 30.5 41.0 48-49 36.06974430828827 39.0 36.0 41.0 29.5 41.0 50-51 35.82531635439992 39.0 35.0 41.0 28.5 41.0 52-53 35.60185915171526 39.0 35.0 40.5 28.0 41.0 54-55 35.37295623720912 39.0 35.0 40.0 27.5 41.0 56-57 35.115104246165885 38.5 35.0 40.0 26.5 41.0 58-59 34.87198112913958 38.0 34.0 40.0 26.0 41.0 60-61 34.53108860887052 38.0 34.0 40.0 26.0 41.0 62-63 34.17973664672486 37.5 34.0 40.0 25.5 41.0 64-65 33.83197650552685 37.0 33.5 40.0 24.5 41.0 66-67 33.3914184562172 36.5 33.0 39.0 23.0 40.5 68-69 32.98933642265748 36.0 32.5 39.0 22.0 40.0 70-71 32.58326430022659 35.5 32.0 38.5 21.5 40.0 72-73 32.1339895553774 35.0 32.0 38.0 20.0 40.0 74-75 31.690277594595116 35.0 31.0 37.0 18.5 39.0 76-77 30.37694669656208 33.5 29.5 35.5 18.0 38.5 78-79 31.002038761286727 34.0 31.0 36.0 18.0 38.5 80-81 30.86020721787441 34.0 31.0 36.0 17.5 37.5 82-83 30.520417992374 34.0 31.0 35.5 13.0 37.0 84-85 30.145757168452143 34.0 30.5 35.0 7.5 37.0 86-87 29.77872048927307 34.0 30.0 35.0 6.0 36.0 88-89 29.365595875500336 34.0 29.5 35.0 2.0 36.0 90-91 28.921847237020913 34.0 29.0 35.0 2.0 35.0 92-93 28.561653356490915 34.0 29.0 35.0 2.0 35.0 94-95 28.136000530529923 34.0 29.0 35.0 2.0 35.0 96-97 27.62756058488701 33.0 27.5 35.0 2.0 35.0 98-99 27.126052353522617 33.0 27.0 35.0 2.0 35.0 100-101 25.2017347439304 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19647379850772495 1101 2 0.17781005383601212 1101 3 0.15068375506088927 1101 4 0.04154204015723195 1101 5 -0.035902897058328165 1101 6 -0.016538899907516225 1101 7 -0.058879243988059216 1101 8 0.06364802077251142 1101 9 0.0814635999686999 1101 10-11 0.028120539901657082 1101 12-13 0.10387442204581987 1101 14-15 0.04374947907537319 1101 16-17 -0.03779427269044078 1101 18-19 -0.007904014779036572 1101 20-21 0.01690503380519459 1101 22-23 -0.07723838058209509 1101 24-25 0.08770337059736732 1101 26-27 -0.02814527052201754 1101 28-29 -0.09221605110960951 1101 30-31 -0.018717019308553517 1101 32-33 -0.006912035072055289 1101 34-35 -0.12467704981867911 1101 36-37 -0.15984878759289245 1101 38-39 -0.1635376052105002 1101 40-41 -0.24015231193744313 1101 42-43 0.0191982229424994 1101 44-45 0.06952983224348941 1101 46-47 0.16857660576727795 1101 48-49 0.05245337324498678 1101 50-51 -0.1093962172963927 1101 52-53 -0.1177520246104109 1101 54-55 -0.09753650993740592 1101 56-57 0.005988490864908158 1101 58-59 0.01295512490055728 1101 60-61 0.13077270441215916 1101 62-63 0.029107298955828753 1101 64-65 0.05755497723450276 1101 66-67 -0.1569786470267971 1101 68-69 -0.16542286631742797 1101 70-71 -0.32485598282111994 1101 72-73 -0.2525220047405341 1101 74-75 -0.18966669604268915 1101 76-77 -0.1623956473543089 1101 78-79 -0.08431304999121991 1101 80-81 -0.02753499256549219 1101 82-83 -0.2502331551321433 1101 84-85 -0.03863307573358554 1101 86-87 -0.25262816421578194 1101 88-89 -0.24620029585272718 1101 90-91 -0.2082720585128115 1101 92-93 -0.18314466186915723 1101 94-95 -0.1533714656301548 1101 96-97 -0.2382662411406038 1101 98-99 -0.1338687877888134 1101 100-101 0.29372779092117085 1102 1 0.10980781792386551 1102 2 0.15221573460168258 1102 3 0.1296021455106171 1102 4 0.14234132663111154 1102 5 0.014157135187559788 1102 6 0.0582629936810406 1102 7 0.04051528793194592 1102 8 0.141089617973293 1102 9 0.13253388915260444 1102 10-11 0.12964707415434162 1102 12-13 0.0527123692860485 1102 14-15 0.09726994149518475 1102 16-17 0.0341140666461186 1102 18-19 0.20175431598148919 1102 20-21 0.20360617613307141 1102 22-23 0.15691567931292383 1102 24-25 0.19392099606476165 1102 26-27 0.04636589547572356 1102 28-29 0.03898613749455393 1102 30-31 -0.04463894308474892 1102 32-33 -0.05015840740919231 1102 34-35 0.02495246546381935 1102 36-37 -0.023183664612552946 1102 38-39 -0.21109257759567157 1102 40-41 -0.07583033327461663 1102 42-43 -0.06598410356431117 1102 44-45 -0.2530800267664901 1102 46-47 -0.16474729392741239 1102 48-49 -0.15582507346353225 1102 50-51 -0.15411595246843035 1102 52-53 -0.10242664983767469 1102 54-55 -0.15220701866780217 1102 56-57 -0.25046926358605504 1102 58-59 -0.28617612274103266 1102 60-61 -0.37535830310044105 1102 62-63 -0.5585766569976514 1102 64-65 -0.5859143720159494 1102 66-67 -0.37993583545944176 1102 68-69 -0.16442032781102967 1102 70-71 -0.24046007873549513 1102 72-73 -0.102835887626199 1102 74-75 -0.3029106670899928 1102 76-77 -0.17191590394928014 1102 78-79 -0.017138682045008125 1102 80-81 0.041789387387169086 1102 82-83 -0.020916836652503434 1102 84-85 -0.13740498750333785 1102 86-87 -0.14885127834565637 1102 88-89 -0.056577298356927486 1102 90-91 0.023796309639227076 1102 92-93 -0.12025059305505081 1102 94-95 -0.03171783453688093 1102 96-97 -0.09612960774895285 1102 98-99 -0.058953690536576175 1102 100-101 0.3370805060938906 1103 1 0.13398142524038548 1103 2 0.1707981862103125 1103 3 0.1625612632891702 1103 4 0.13521163720793794 1103 5 -0.015849101738353966 1103 6 -0.08212579309888213 1103 7 -0.06263546353483918 1103 8 0.1372191362949522 1103 9 0.03972925947697803 1103 10-11 0.04414806488726697 1103 12-13 8.492946471534424E-4 1103 14-15 0.11064000645856709 1103 16-17 0.0011935846918120774 1103 18-19 -0.0011624127784628513 1103 20-21 -0.008986756822729092 1103 22-23 -0.04518762736168469 1103 24-25 0.049654138426738825 1103 26-27 0.14845140207416563 1103 28-29 -0.08622015020987561 1103 30-31 -0.014812776696814467 1103 32-33 -0.11798497671357921 1103 34-35 -0.03651782082763333 1103 36-37 -0.15195479702855863 1103 38-39 0.03693009611375686 1103 40-41 -0.01864793354837957 1103 42-43 0.056256194704253915 1103 44-45 -0.12818558535771984 1103 46-47 -0.09691382536868787 1103 48-49 0.009054686332028439 1103 50-51 0.07102327448392032 1103 52-53 0.20996330705578004 1103 54-55 0.0021017036454864524 1103 56-57 -0.07044426068836884 1103 58-59 -0.04188387584707698 1103 60-61 -0.1523150696131239 1103 62-63 -0.19268367271139653 1103 64-65 -0.21230181206142618 1103 66-67 -0.06451020666140295 1103 68-69 -0.042832699001948527 1103 70-71 -0.1140857472732435 1103 72-73 0.13729748875848458 1103 74-75 0.06392130571038734 1103 76-77 0.13817453147645864 1103 78-79 0.18692815241152516 1103 80-81 0.12960792595951887 1103 82-83 -0.09515074345185681 1103 84-85 0.07963520479268027 1103 86-87 0.15462346210439648 1103 88-89 0.25371183559347443 1103 90-91 0.1680238415275781 1103 92-93 0.27755489212811213 1103 94-95 0.20246631484025457 1103 96-97 0.2360432710063094 1103 98-99 0.1962104013283792 1103 100-101 0.3874747294862253 1104 1 0.1374331441401182 1104 2 0.14049451635322896 1104 3 0.12481496588456764 1104 4 0.04074890989272717 1104 5 -0.006049717510130392 1104 6 0.05503443067742353 1104 7 0.007544127354314867 1104 8 0.06148131818932967 1104 9 0.11830925578858142 1104 10-11 0.09584369855582509 1104 12-13 0.033766250883338955 1104 14-15 -0.02973148673522985 1104 16-17 0.020917979836994505 1104 18-19 -0.052882418024637445 1104 20-21 -0.15024869198629887 1104 22-23 0.012634568663187906 1104 24-25 0.004275014667861399 1104 26-27 -0.04998399289363675 1104 28-29 -0.044065361044118845 1104 30-31 0.14194948182984746 1104 32-33 0.08071574182082486 1104 34-35 0.13568119395761613 1104 36-37 0.20048158184746967 1104 38-39 0.21801544035348996 1104 40-41 0.26705378949703373 1104 42-43 0.19517921217691736 1104 44-45 0.16924640412180025 1104 46-47 0.21591823737631444 1104 48-49 0.12396416225265483 1104 50-51 -0.1002644443012457 1104 52-53 -0.13442743616678143 1104 54-55 0.0048602144767713185 1104 56-57 0.11447119003757678 1104 58-59 0.13445303081179105 1104 60-61 0.017026440510129248 1104 62-63 0.021498776436182254 1104 64-65 -0.16168229660858913 1104 66-67 -0.05789194144537646 1104 68-69 0.08337689757055244 1104 70-71 0.05516631664763594 1104 72-73 0.009317560888298715 1104 74-75 -0.03978779714922709 1104 76-77 0.07034943598761245 1104 78-79 0.08372633012102781 1104 80-81 0.1258230450812583 1104 82-83 0.1729351027921311 1104 84-85 0.2111085596232556 1104 86-87 0.32753327269452726 1104 88-89 0.2557806559035889 1104 90-91 0.255327103851684 1104 92-93 0.08340964858067323 1104 94-95 0.010758480307689666 1104 96-97 0.2610949915140601 1104 98-99 0.23924231603488977 1104 100-101 0.4130332280563458 1105 1 0.1337290186743374 1105 2 0.22994139647368428 1105 3 0.12958129914830607 1105 4 0.1225060128933535 1105 5 0.12414254273394221 1105 6 0.04129405476088266 1105 7 0.05761972820711492 1105 8 0.048333728875938675 1105 9 0.1449062051834602 1105 10-11 0.11137002599924983 1105 12-13 0.18414868364919812 1105 14-15 0.18812360003177275 1105 16-17 0.24251515065696339 1105 18-19 0.1253211285726863 1105 20-21 0.15083882153644623 1105 22-23 0.14476746167691346 1105 24-25 0.054589121976789556 1105 26-27 0.044604377860544275 1105 28-29 0.031085366179148366 1105 30-31 0.10985865958357977 1105 32-33 0.194567462926436 1105 34-35 0.1047262824327575 1105 36-37 0.10942525763052657 1105 38-39 0.056783178084963026 1105 40-41 0.13137208490341123 1105 42-43 -0.024432091890346896 1105 44-45 0.19468941246636717 1105 46-47 0.1576521223744436 1105 48-49 0.1587256874286922 1105 50-51 -0.007732796329179337 1105 52-53 -0.060681809312349344 1105 54-55 -0.26522557193026586 1105 56-57 -0.1662499598545537 1105 58-59 -0.10076336476729608 1105 60-61 -0.0011244069153306668 1105 62-63 -0.08211741163596997 1105 64-65 -0.31301464301866133 1105 66-67 -0.2842614556387204 1105 68-69 -0.08915974828215667 1105 70-71 -0.08000041644656619 1105 72-73 -0.19122967541022717 1105 74-75 -0.1742469189140472 1105 76-77 -0.3707641424316641 1105 78-79 -0.16224499849278828 1105 80-81 -0.26771134223551485 1105 82-83 -0.14471817081969363 1105 84-85 -0.06653256724067091 1105 86-87 0.09248705811463154 1105 88-89 0.12821006874751006 1105 90-91 0.23030037467987796 1105 92-93 0.22093139568791287 1105 94-95 0.29754003496488934 1105 96-97 0.23476470119413406 1105 98-99 0.32342592172108553 1105 100-101 0.6234313503715647 1106 1 -0.05824994253539728 1106 2 0.016397675237655562 1106 3 -0.024725078234993703 1106 4 0.0720129162464147 1106 5 -0.02201130341990165 1106 6 -0.11735157311551347 1106 7 0.09384589520251296 1106 8 0.10954345931644127 1106 9 -0.03313455747269245 1106 10-11 -0.15957014494092192 1106 12-13 -0.10480595145928362 1106 14-15 0.055250490235579264 1106 16-17 0.06444151489102268 1106 18-19 0.018779379723248724 1106 20-21 -0.03949648418346641 1106 22-23 0.17662150506941288 1106 24-25 0.21780411211485529 1106 26-27 0.06735256983950677 1106 28-29 0.23913927807921453 1106 30-31 0.08339580619765741 1106 32-33 0.08651355102636415 1106 34-35 0.010117472439333142 1106 36-37 0.1432845607205948 1106 38-39 0.1527726586898268 1106 40-41 0.032522643746276 1106 42-43 -0.31575359090415134 1106 44-45 -0.09153675452903798 1106 46-47 0.14933522559601187 1106 48-49 -0.12362145261075597 1106 50-51 -0.24086889100373554 1106 52-53 -0.30591520839190167 1106 54-55 -0.46709933918864266 1106 56-57 -0.4100040689932811 1106 58-59 -0.34658847982318974 1106 60-61 -0.4502368329508357 1106 62-63 -0.5310654721224921 1106 64-65 -0.6943755897642205 1106 66-67 -0.7292647429496952 1106 68-69 -0.36295987977459276 1106 70-71 -0.49932980500671675 1106 72-73 -0.4609864143977376 1106 74-75 -0.6713540852519841 1106 76-77 -0.4771086526223307 1106 78-79 -0.38703132327911405 1106 80-81 -0.5204069372387998 1106 82-83 -0.589820077460061 1106 84-85 -0.8974332904490865 1106 86-87 -0.6712210286703773 1106 88-89 -0.6245183993655701 1106 90-91 -0.7450776660827785 1106 92-93 -0.6616787292299051 1106 94-95 -1.2833935613533214 1106 96-97 -0.7965633724416321 1106 98-99 -0.7587239796595853 1106 100-101 -0.3754180907855691 1107 1 0.15509654004186046 1107 2 0.14468498621727122 1107 3 0.0043676239346339685 1107 4 0.151565841953186 1107 5 0.06132202991343405 1107 6 0.0810376445044696 1107 7 0.057192377779763603 1107 8 0.05431663485884286 1107 9 0.1771711624484169 1107 10-11 0.12141276104198795 1107 12-13 0.18308030758081628 1107 14-15 0.07957659148476637 1107 16-17 0.10084848399029767 1107 18-19 0.09455189780346274 1107 20-21 0.23844565914328797 1107 22-23 0.32927600868545426 1107 24-25 0.2111062159938868 1107 26-27 0.1590274547836188 1107 28-29 0.1862135713073485 1107 30-31 0.08314925787418304 1107 32-33 0.21048626634523515 1107 34-35 0.4098544875609633 1107 36-37 0.1473526080578722 1107 38-39 0.10432591312769546 1107 40-41 -0.03540141937009622 1107 42-43 0.09885850640025495 1107 44-45 0.3185141987911493 1107 46-47 -0.14085215112982752 1107 48-49 -0.07866747496446891 1107 50-51 0.05252361392722804 1107 52-53 0.06111306248252646 1107 54-55 -0.04546061466530915 1107 56-57 -0.25065166925626414 1107 58-59 -0.133241997245932 1107 60-61 -0.023132953923877153 1107 62-63 -0.18799348001203953 1107 64-65 0.11508364757963108 1107 66-67 0.2759949546176941 1107 68-69 0.07622486710246079 1107 70-71 0.07619616475001578 1107 72-73 0.026932717752167434 1107 74-75 -0.007687089854218954 1107 76-77 0.07902218235465952 1107 78-79 0.034443035695247204 1107 80-81 0.037737375713206234 1107 82-83 0.17045276935124676 1107 84-85 0.3882751250670182 1107 86-87 0.38137594700352295 1107 88-89 0.3681673337047755 1107 90-91 0.4575439644234649 1107 92-93 0.3286237033802202 1107 94-95 0.31506140248626124 1107 96-97 0.3011108550402888 1107 98-99 0.5571866054817676 1107 100-101 1.2537732307134455 1108 1 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11 1979.0 12 3173.0 13 4576.0 14 5937.0 15 8608.0 16 12071.0 17 17130.0 18 14841.0 19 8293.0 20 6650.0 21 6590.0 22 6735.0 23 7347.0 24 8296.0 25 9867.0 26 12101.0 27 14891.0 28 18798.0 29 23509.0 30 29750.0 31 37443.0 32 48107.0 33 63513.0 34 87336.0 35 125401.0 36 192827.0 37 296793.0 38 249528.0 39 24960.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.791763572859985 20.01923172448664 15.196472750485057 20.992531952168314 2 15.562606235653092 21.64865878182624 39.676154458303756 23.112580524216913 3 19.990604581822385 25.79301627007826 30.3223043374526 23.894074810646757 4 12.846604238015285 17.336769428435513 37.66562388392361 32.151002449625594 5 12.824187844419477 38.829396193192096 33.95209030571259 14.394325656675846 6 30.701233111587634 37.49201611743976 17.21421405252246 14.592536718450141 7 27.64809268565213 34.19924807016036 20.595601343811545 17.557057900375963 8 26.66824244921065 34.71243944475153 19.438883101140046 19.18043500489777 9 26.56799007701575 17.010671357460094 20.671179628836526 35.750158936687626 10-11 24.742922686378275 27.604116497257692 26.925245666474513 20.727715149889523 12-13 26.2585152974329 24.979428427042514 27.86678438435145 20.895271891173138 14-15 22.751842405938376 26.11881795562221 27.333812983756594 23.795526654682817 16-17 21.042105996321855 29.583156119544103 27.69921917258079 21.675518711553252 18-19 20.91114068379083 27.70944446996652 30.984762824968104 20.394652021274545 20-21 22.11624384814989 26.332104321744172 31.090238990392667 20.461412839713276 22-23 21.561713182303855 26.46760256138567 31.057912746317008 20.912771509993462 24-25 21.526579884883727 26.514791136876926 31.330654005062268 20.62797497317708 26-27 21.34826340481357 26.615900274196314 31.429062586485205 20.60677373450491 28-29 20.947079708509918 27.129591859796072 31.367083139286954 20.556245292407052 30-31 21.19059509748858 26.642531013030585 31.22576122893255 20.941112660548285 32-33 21.127279759690694 26.752045429958937 31.178302511718336 20.942372298632034 34-35 21.617871745132238 26.69599153523208 31.05207936609084 20.634057353544843 36-37 21.608202170430516 26.39953200740371 31.267736815664566 20.724529006501214 38-39 21.19818997417001 26.92572729280065 31.167669684364334 20.708413048665005 40-41 21.34782756888368 27.09518566324391 30.874062866313867 20.682923901558542 42-43 21.188817096568368 27.107373035911063 30.517030688067514 21.18677917945305 44-45 21.457601323064566 26.992525159418314 30.38843533684945 21.16143818066767 46-47 21.928780062744796 27.417060241820874 29.72542112664994 20.928738568784393 48-49 21.89895627870308 27.558473140811234 29.385800470363684 21.156770110122007 50-51 21.586714226648905 27.940328721082043 29.415216724437126 21.057740327831926 52-53 21.93300355514325 28.236121381689607 28.668066099878924 21.162808963288217 54-55 21.559335622416818 28.03487567372113 28.83426423057601 21.57152447328604 56-57 21.919258680758805 28.113491909418688 28.651727852969117 21.315521556853394 58-59 22.327047986283283 27.68462219008087 28.651505563895512 21.336824259740336 60-61 21.798666858329245 27.88905404143765 29.397396550369965 20.914882549863144 62-63 21.612388614650037 27.82818388344939 29.49142482850398 21.06800267339659 64-65 21.594012717898863 28.12931148182342 29.047402403982236 21.22927339629548 66-67 21.700155750544237 27.909023009882972 28.750905827974933 21.63991541159786 68-69 21.63613649734661 27.931992880821937 28.969267794610825 21.462602827220632 70-71 21.843680395733827 28.03591302273128 28.697334161236636 21.423072420298254 72-73 21.891393843472695 27.661767408224115 28.94797094232924 21.498867805973955 74-75 21.589085310100668 28.662953451186063 28.306438825305978 21.44152241340729 76-77 21.762804221121314 27.995160025904088 28.594303175621704 21.647732577352894 78-79 21.82463763176185 27.9163955974908 28.45374238474682 21.80522438600053 80-81 21.836085519052396 28.026725074355696 28.44159058205653 21.695598824535377 82-83 21.804577017991754 28.025834503132618 28.668807315996965 21.500781162878667 84-85 21.577266333006193 28.124547311358278 28.505370762880624 21.79281559275491 86-87 21.442819099669975 28.084742522566046 28.391760781390552 22.080677596373427 88-89 21.5965690422453 28.035727781836613 28.177400018079513 22.190303157838578 90-91 21.54583769463768 27.781913169132157 28.334540604129423 22.337708532100745 92-93 21.450662908468765 27.822038362961006 28.475017838843552 22.252280889726677 94-95 21.34890196607276 27.80313931449013 28.55062337265874 22.297335346778365 96-97 21.163549926866896 27.791839619915322 28.75149859883787 22.29311185437991 98-99 21.160980073614812 27.937803448077847 28.184692786896786 22.716523691410558 100-101 20.773039223314786 28.171068164487593 28.379489961294684 22.676402650902936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 591.0 1 424.0 2 316.5 3 403.0 4 349.0 5 340.5 6 662.5 7 1304.5 8 2061.0 9 2496.5 10 2353.0 11 2141.0 12 2262.5 13 2466.0 14 2625.5 15 2761.5 16 2897.5 17 3057.5 18 3221.0 19 3407.5 20 3709.5 21 4115.0 22 4772.5 23 5513.0 24 6319.5 25 7565.0 26 9422.0 27 11667.0 28 13795.5 29 15995.0 30 18386.0 31 21171.0 32 24004.0 33 26288.5 34 29273.5 35 32022.0 36 35138.5 37 38958.0 38 40425.0 39 40890.5 40 41842.0 41 43070.5 42 44142.5 43 45743.5 44 48456.5 45 50180.5 46 50708.0 47 54106.0 48 61586.0 49 59773.0 50 50708.0 51 45290.0 52 41686.5 53 40193.5 54 38673.5 55 35065.5 56 30317.0 57 26246.5 58 23622.5 59 20702.0 60 17009.5 61 13791.0 62 11018.5 63 8401.5 64 5998.5 65 4333.5 66 3150.0 67 2291.5 68 1646.5 69 1145.5 70 837.5 71 621.5 72 474.0 73 428.5 74 345.5 75 268.5 76 182.5 77 97.0 78 70.5 79 40.0 80 21.5 81 15.0 82 6.5 83 3.5 84 1.5 85 0.5 86 0.5 87 1.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05786925549461541 2 0.0 3 0.0 4 0.0 5 0.01644939144661283 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0027786134200359514 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.891562944115045E-4 24-25 1.4819271573525075E-4 26-27 0.0010003008312129426 28-29 0.0033713842829769546 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013115055342569692 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 1.4819271573525075E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.593372525366888E-4 84-85 0.0013707826205510696 86-87 0.0 88-89 0.0 90-91 0.009743671059592736 92-93 8.15059936543879E-4 94-95 0.0 96-97 0.0 98-99 1.1114453680143806E-4 100-101 0.012374091763893438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1349594.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.20388845202924 #Duplication Level Percentage of deduplicated Percentage of total 1 78.403000087288 45.6335947138495 2 12.487637005669974 14.53658062614895 3 3.929677747688164 6.8616757583659025 4 1.7113774534102444 3.9843528959043093 5 0.9275692985225207 2.69940699913659 6 0.5586236679151031 1.9508441792396451 7 0.3642329935718969 1.4839843569885158 8 0.26732651278351116 1.244755402425716 9 0.1924590722684737 1.0081679736505749 >10 1.0577285641145349 11.212667167000975 >50 0.06453851187039737 2.5568140160795076 >100 0.03263009129278672 3.294239002832517 >500 0.0015355169301530102 0.6040873818165127 >1k 0.0012795974417941752 1.3336694299332132 >5k 3.8387923253825254E-4 1.5951600966275514 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9029 0.6690160151867894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7191 0.5328269094260941 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5198 0.3851528681959167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4837 0.3584040830057039 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1994 0.147748137588045 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1811 0.13418850409826955 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1810 0.13411440774040193 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.10588369539283667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.409635786762538E-5 0.0 4 0.0 0.0 0.0 7.409635786762538E-5 0.0 5 0.0 0.0 0.0 7.409635786762538E-5 0.0 6 0.0 0.0 0.0 7.409635786762538E-5 0.0 7 0.0 0.0 0.0 7.409635786762538E-5 0.0 8 0.0 0.0 0.0 7.409635786762538E-5 0.0 9 0.0 0.0 0.0 7.409635786762538E-5 0.0 10-11 7.409635786762538E-5 0.0 0.0 7.409635786762538E-5 0.0 12-13 1.4819271573525075E-4 0.0 0.0 2.963854314705015E-4 0.0 14-15 1.4819271573525075E-4 0.0 0.0 2.963854314705015E-4 0.0 16-17 1.4819271573525075E-4 0.0 0.0 2.963854314705015E-4 0.0 18-19 1.4819271573525075E-4 0.0 0.0 2.963854314705015E-4 0.0 20-21 1.4819271573525075E-4 0.0 0.0 2.963854314705015E-4 0.0 22-23 1.4819271573525075E-4 0.0 0.0 4.075299682719395E-4 0.0 24-25 1.4819271573525075E-4 0.0 0.0 7.409635786762537E-4 0.0 26-27 1.4819271573525075E-4 0.0 0.0 8.521081154776917E-4 0.0 28-29 1.4819271573525075E-4 0.0 0.0 0.0021117461992273233 0.0 30-31 1.4819271573525075E-4 0.0 0.0 0.005334937766469027 0.0 32-33 1.4819271573525075E-4 0.0 0.0 0.010299393743599927 0.0 34-35 1.4819271573525075E-4 0.0 0.0 0.01611595783620852 0.0 36-37 1.4819271573525075E-4 0.0 0.0 0.020635835666133667 0.0 38-39 1.4819271573525075E-4 0.0 0.0 0.02497047260138975 0.0 40-41 1.4819271573525075E-4 0.0 0.0 0.0335656501140343 0.0 42-43 1.4819271573525075E-4 0.0 0.0 0.04523582647818529 0.0 44-45 1.4819271573525075E-4 3.704817893381269E-5 0.0 0.05920298993623267 0.0 46-47 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.07320720157321386 0.0 48-49 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.0873225577469965 0.0 50-51 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.10929212785474743 0.0 52-53 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.15604692966921904 0.0 54-55 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.21269359525901865 0.0 56-57 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.26411646761915064 0.0 58-59 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.3562552886275428 0.0 60-61 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.47625434019416213 0.0 62-63 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.5554633467546536 0.0 64-65 1.4819271573525075E-4 7.409635786762538E-5 0.0 0.6239283814243395 0.0 66-67 2.2228907360287613E-4 7.409635786762538E-5 0.0 0.7993144604970088 0.0 68-69 2.2228907360287613E-4 7.409635786762538E-5 0.0 0.9679207228247902 0.0 70-71 2.2228907360287613E-4 7.409635786762538E-5 0.0 1.0536502088776327 0.0 72-73 2.2228907360287613E-4 7.409635786762538E-5 0.0 1.2307034560023236 0.0 74-75 2.2228907360287613E-4 7.409635786762538E-5 0.0 1.4831497472573232 0.0 76-77 2.2228907360287613E-4 7.409635786762538E-5 0.0 1.7287421254095676 0.0 78-79 2.2228907360287613E-4 7.409635786762538E-5 0.0 1.9985640125845254 0.0 80-81 2.2228907360287613E-4 7.409635786762538E-5 0.0 2.3086572702605377 0.0 82-83 2.2228907360287613E-4 7.409635786762538E-5 0.0 2.5775900011410835 0.0 84-85 2.2228907360287613E-4 7.409635786762538E-5 0.0 2.861823629921295 0.0 86-87 2.2228907360287613E-4 7.409635786762538E-5 0.0 3.294101781721021 0.0 88-89 2.2228907360287613E-4 7.409635786762538E-5 0.0 3.87546180555041 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 7450 0.0 24.295805 2 GTACATG 7515 0.0 24.289713 1 TAACGCA 100 0.001652267 23.75377 4 GTATCAA 13885 0.0 23.656599 1 ACATGGG 7350 0.0 23.398272 3 GAGTACT 7805 0.0 22.851732 12-13 GGTATCA 10540 0.0 21.64815 1 GTACTTT 8305 0.0 21.246391 14-15 AGTACTT 8020 0.0 20.96578 12-13 CATGGGA 5080 0.0 20.854689 4 CCCTATA 230 9.008363E-8 20.655453 2 ATCAACG 16350 0.0 20.078112 3 TCAACGC 16375 0.0 20.07647 4 CAACGCA 16515 0.0 19.906279 5 TATCAAC 16540 0.0 19.904913 2 AACGCAG 16735 0.0 19.696983 6 AGAGTAC 12580 0.0 19.671577 10-11 GTAAGGT 435 0.0 19.658293 4 TACCTGG 1005 0.0 19.381187 2 ACTTTTT 9570 0.0 18.586853 16-17 >>END_MODULE