FastQCFastQC Report
Thu 26 May 2016
SRR937428_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937428_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382610
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69500.5026724817555204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52480.379571968957262No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40780.2949494072804334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.24432052422592052No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT27170.19651239322730196No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20110.14544954831803616No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT19640.14205018045580461No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC16290.11782064356543061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG73700.025.403971
TACATGG73450.025.2926772
GTATCAA123350.024.4243471
ACATGGG74100.023.7242983
TACCTGG10350.022.9530032
GAGTACT61800.022.44367412-13
GTCGGGA3251.8189894E-1221.9289472
CATGGGA49100.020.8057144
ATCAACG142550.020.764943
TCAACGC143150.020.7450394
GTACTTT67150.020.72439214-15
CAACGCA143450.020.6670325
TATCAAC144050.020.6476632
AGTACTT64850.020.6190212-13
AACGCAG145950.020.3433636
CATGGGG25550.020.084384
GTATACG1200.0047526119.7998371
GGTATCA92350.019.5018221
ATGGGAG19950.019.2901765
AGAGTAC109050.019.2753810-11
Produced by FastQC (version 0.11.5)