##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937428_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1382610 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.810643637757575 33.0 31.0 34.0 30.0 34.0 2 31.89661075791438 33.0 31.0 34.0 30.0 34.0 3 31.941917098820348 33.0 31.0 34.0 30.0 34.0 4 35.54177750775707 37.0 35.0 37.0 33.0 37.0 5 35.566302861978436 37.0 35.0 37.0 33.0 37.0 6 35.602754211238164 37.0 35.0 37.0 33.0 37.0 7 35.49456752084825 37.0 35.0 37.0 33.0 37.0 8 35.509383701839276 37.0 35.0 37.0 33.0 37.0 9 37.14071357794317 39.0 37.0 39.0 34.0 39.0 10-11 37.03654428942363 39.0 37.0 39.0 33.0 39.0 12-13 36.90225768654935 39.0 37.0 39.0 33.0 39.0 14-15 38.07354532369938 40.0 38.0 41.0 33.0 41.0 16-17 38.021273533389746 40.0 38.0 41.0 33.0 41.0 18-19 38.00445244862976 40.0 38.0 41.0 33.0 41.0 20-21 37.906167321225794 40.0 38.0 41.0 33.0 41.0 22-23 37.75258134976602 40.0 37.5 41.0 32.5 41.0 24-25 37.69551898221479 40.0 37.5 41.0 32.5 41.0 26-27 37.72216279355712 40.0 37.5 41.0 33.0 41.0 28-29 37.6501048741149 40.0 37.0 41.0 33.0 41.0 30-31 37.44184658001895 40.0 37.0 41.0 32.0 41.0 32-33 37.20490593876798 40.0 36.5 41.0 31.5 41.0 34-35 37.21987979256623 40.0 37.0 41.0 32.0 41.0 36-37 37.093475745148666 40.0 36.5 41.0 31.0 41.0 38-39 36.97998604089367 40.0 36.0 41.0 31.0 41.0 40-41 36.776157412430116 39.5 36.0 41.0 30.5 41.0 42-43 36.50217306398768 39.0 36.0 41.0 30.0 41.0 44-45 36.249129183211465 39.0 35.5 41.0 30.0 41.0 46-47 35.890545056089564 39.0 35.0 41.0 28.5 41.0 48-49 35.53734965029907 39.0 35.0 41.0 27.0 41.0 50-51 34.55432226007334 38.0 34.0 39.5 25.0 40.5 52-53 34.67433007138673 38.0 34.5 39.5 26.0 40.5 54-55 35.059541013011625 39.0 35.0 40.0 26.0 41.0 56-57 34.846036481726586 38.5 34.5 40.0 25.5 41.0 58-59 34.58654862904217 38.0 34.0 40.0 24.0 41.0 60-61 34.27305530843839 38.0 34.0 40.0 23.0 41.0 62-63 33.97250092216894 38.0 34.0 40.0 22.5 41.0 64-65 33.56180629389343 37.0 33.0 40.0 22.0 41.0 66-67 33.1968700501226 36.5 33.0 39.5 20.5 41.0 68-69 32.71786186994163 36.0 32.0 39.0 18.5 40.5 70-71 32.23427105257448 35.5 32.0 39.0 17.0 40.0 72-73 31.78321977998134 35.0 31.0 38.0 12.5 40.0 74-75 31.303764619089982 35.0 31.0 37.0 8.0 39.5 76-77 30.820618974258828 35.0 30.5 37.0 7.0 39.0 78-79 30.313197503272796 34.5 30.0 36.0 2.0 39.0 80-81 29.80736397104028 34.0 29.5 36.0 2.0 37.5 82-83 29.325667758804002 34.0 29.0 35.0 2.0 37.0 84-85 28.809303057261268 34.0 29.0 35.0 2.0 36.5 86-87 28.351133363710662 34.0 28.5 35.0 2.0 36.0 88-89 27.877128040445246 33.5 27.0 35.0 2.0 36.0 90-91 27.366638820780985 33.0 26.0 35.0 2.0 35.0 92-93 26.866200880942564 33.0 25.0 35.0 2.0 35.0 94-95 26.32959945320806 33.0 25.0 35.0 2.0 35.0 96-97 25.61069028865696 32.0 22.0 34.5 2.0 35.0 98-99 24.697180332848742 32.0 10.0 34.0 2.0 35.0 100-101 22.12187059257491 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.013083304204808854 1101 2 0.012710429910658405 1101 3 -0.04918483547400854 1101 4 0.0547612067751615 1101 5 -0.023943689941631874 1101 6 -0.03601932216290038 1101 7 0.02841151386596863 1101 8 -0.12188358178305236 1101 9 0.06668809782289742 1101 10-11 -0.22671478346882878 1101 12-13 -0.07626521753288529 1101 14-15 -0.2274303257444572 1101 16-17 -0.21651988854749504 1101 18-19 0.1182150322665052 1101 20-21 0.09677245221373454 1101 22-23 -0.09566791261072893 1101 24-25 -0.11797586625459644 1101 26-27 -0.1482459362914028 1101 28-29 -0.12321905150887602 1101 30-31 -0.1622673541863051 1101 32-33 -0.32460300308832757 1101 34-35 -0.154040463941719 1101 36-37 -2.275646233655948E-4 1101 38-39 -0.07727175419877597 1101 40-41 -0.22434551446890083 1101 42-43 -0.29171634563995497 1101 44-45 -0.1883642952678457 1101 46-47 -0.2636246342500854 1101 48-49 -0.20989469686527684 1101 50-51 -0.20762651057999193 1101 52-53 -0.2661477906439558 1101 54-55 -0.3717874797868248 1101 56-57 -0.340330169436065 1101 58-59 -0.5032531891915966 1101 60-61 -0.2923275002839105 1101 62-63 -0.35962644487919704 1101 64-65 -0.4420967688976418 1101 66-67 -0.464160688827711 1101 68-69 -0.3220782133596174 1101 70-71 -0.5771429145339084 1101 72-73 -0.48723040541602813 1101 74-75 -0.11473836891761735 1101 76-77 -0.25032737558955986 1101 78-79 -0.29548285229362037 1101 80-81 -0.0515191997762976 1101 82-83 -0.12305866183913494 1101 84-85 -0.15738303788241836 1101 86-87 -0.14849477307722125 1101 88-89 -0.23623642456734828 1101 90-91 -0.13437361323339658 1101 92-93 0.021946378245434772 1101 94-95 -0.08473353637617365 1101 96-97 -0.09934427387784339 1101 98-99 0.011118938118364952 1101 100-101 0.39114309196070707 1102 1 -0.20098900948820742 1102 2 -0.22071192654859217 1102 3 -0.09158420908557119 1102 4 -0.22151453779311225 1102 5 -0.18616293209566948 1102 6 -0.2932677945143496 1102 7 -0.19532600374948572 1102 8 -0.23987292449888997 1102 9 -0.2620411335112678 1102 10-11 -0.19601937311447415 1102 12-13 -0.21831026693193678 1102 14-15 -0.08318616515285271 1102 16-17 -0.14859494492900183 1102 18-19 -0.2258082526834002 1102 20-21 -0.16287148054389178 1102 22-23 -0.19880619309454772 1102 24-25 -0.10015166356157579 1102 26-27 -0.21750743245174675 1102 28-29 -0.12831379689389166 1102 30-31 -0.08011708227709136 1102 32-33 -0.21134602089306753 1102 34-35 -0.10874872862459029 1102 36-37 -0.15298036337471643 1102 38-39 -0.1814477575002016 1102 40-41 -0.2590462765449786 1102 42-43 -0.27443434235437536 1102 44-45 -0.1569665058844194 1102 46-47 -0.27406662321939734 1102 48-49 -0.5005940135355615 1102 50-51 -0.5960646765956596 1102 52-53 -0.41296533158707405 1102 54-55 -0.40762878966342697 1102 56-57 -0.3879762007189669 1102 58-59 -0.4276945670670287 1102 60-61 -0.21393180425867087 1102 62-63 -0.14310596651740326 1102 64-65 -0.24401020920799255 1102 66-67 -0.3234918062855243 1102 68-69 -0.33273170832394783 1102 70-71 -0.30179569506267967 1102 72-73 -0.12559218469815647 1102 74-75 -0.09804472432829314 1102 76-77 -0.09740836895123195 1102 78-79 0.03934715579114467 1102 80-81 -0.013643091996424772 1102 82-83 0.022856361665933633 1102 84-85 -0.10264621644549266 1102 86-87 0.02044391808647461 1102 88-89 -0.1270423209686804 1102 90-91 -0.3521309194048605 1102 92-93 -0.2818887008161255 1102 94-95 -0.11718541101167901 1102 96-97 -0.14481013994494063 1102 98-99 -0.46603979230828685 1102 100-101 0.02069671441424248 1103 1 0.10885451208848806 1103 2 0.10418384474384368 1103 3 0.1468152711245132 1103 4 0.04612312531804719 1103 5 0.13640461352130728 1103 6 0.11284515150330776 1103 7 0.20575147743520716 1103 8 0.15080875832097007 1103 9 0.09580173832803496 1103 10-11 0.1833054414396429 1103 12-13 0.1512414142635734 1103 14-15 0.06276109782836414 1103 16-17 0.08004493811826308 1103 18-19 0.05001880887922283 1103 20-21 0.015914593417882372 1103 22-23 0.12197457467848238 1103 24-25 -0.025865021079582107 1103 26-27 0.026969799666943572 1103 28-29 -0.10917361605031317 1103 30-31 -0.19246750731363704 1103 32-33 -0.16511991227863376 1103 34-35 0.025760448916152257 1103 36-37 -0.02408870708580224 1103 38-39 -0.2074101810206983 1103 40-41 -0.01213252918453378 1103 42-43 -0.03489782169814504 1103 44-45 -0.22352398057172707 1103 46-47 0.07260888709518554 1103 48-49 0.06841351258929507 1103 50-51 -0.12252793936029605 1103 52-53 -0.11235349553747653 1103 54-55 0.0984760346367537 1103 56-57 0.16530226590076325 1103 58-59 0.08643722133515297 1103 60-61 0.0218822625559838 1103 62-63 -0.018951978754124355 1103 64-65 0.14443800130691642 1103 66-67 0.03225295520670102 1103 68-69 0.1097259233832375 1103 70-71 0.25860281054130496 1103 72-73 0.16229562731115976 1103 74-75 0.2821734791478008 1103 76-77 0.38663245621526343 1103 78-79 0.07542111756492531 1103 80-81 -0.19443252293475766 1103 82-83 0.04884585757800508 1103 84-85 -0.09623005964220255 1103 86-87 -0.0177822988175258 1103 88-89 0.19015135492064772 1103 90-91 0.278601659511164 1103 92-93 0.21567478394211648 1103 94-95 -0.06884765585438757 1103 96-97 0.06852030566662037 1103 98-99 0.37657485927003265 1103 100-101 0.891317755076976 1104 1 0.06337656445921169 1104 2 0.12512711954388323 1104 3 0.12022371429484124 1104 4 0.013845675820775227 1104 5 0.156801280382993 1104 6 0.10775350691547203 1104 7 -0.021972577493045264 1104 8 -0.039935681573055604 1104 9 0.1103821289771929 1104 10-11 0.12387561871111785 1104 12-13 0.14588942048187192 1104 14-15 0.11536082870335207 1104 16-17 0.1237685584346977 1104 18-19 0.17333573283051606 1104 20-21 0.052998264550460306 1104 22-23 -0.007049554635251809 1104 24-25 -0.04993541147800329 1104 26-27 0.04930007121573965 1104 28-29 0.05591618979521229 1104 30-31 -0.034094961407767244 1104 32-33 0.05330136029819954 1104 34-35 0.0625127285387066 1104 36-37 -0.056694622821261476 1104 38-39 -0.0719274693772789 1104 40-41 0.32734902906597796 1104 42-43 0.1060747893995071 1104 44-45 0.16669391039085468 1104 46-47 0.16815829493658896 1104 48-49 0.12259372605056029 1104 50-51 -0.12192658704891102 1104 52-53 -0.04641745033784872 1104 54-55 -0.1889745491639303 1104 56-57 -0.3052757678150755 1104 58-59 -0.219236552349102 1104 60-61 -0.15780799294561376 1104 62-63 -0.042531759275156844 1104 64-65 0.037834409073099096 1104 66-67 0.05586951057373568 1104 68-69 -0.10755532896742892 1104 70-71 -0.2098126114918415 1104 72-73 0.03372324354891987 1104 74-75 -0.03450558509952728 1104 76-77 8.939922337170003E-4 1104 78-79 -0.039947007001504886 1104 80-81 0.06740647010309786 1104 82-83 0.11100746631035818 1104 84-85 0.12978939759450725 1104 86-87 0.06679901120278586 1104 88-89 -0.018477779115308124 1104 90-91 0.11180934545397392 1104 92-93 0.201171286152789 1104 94-95 0.16372524609885986 1104 96-97 0.1680706495950126 1104 98-99 0.2116258843688641 1104 100-101 0.7579136580248118 1105 1 0.1764698438825434 1105 2 0.06286637332779321 1105 3 -0.07494657987815145 1105 4 0.17747813568983162 1105 5 0.06523324232433225 1105 6 -2.0368674076820525E-4 1105 7 0.16147422095720287 1105 8 -0.08989491132906124 1105 9 0.0889958441837706 1105 10-11 0.07318035336678719 1105 12-13 0.04573550677968541 1105 14-15 0.016133292142818334 1105 16-17 0.09428588216973566 1105 18-19 0.27154497826737867 1105 20-21 0.14018796439462022 1105 22-23 0.11609081562611578 1105 24-25 0.20169732823414677 1105 26-27 0.2652689796829293 1105 28-29 0.035497293631479465 1105 30-31 0.0972933015868449 1105 32-33 0.047080158806345196 1105 34-35 -0.008675253647830061 1105 36-37 0.07514959142275757 1105 38-39 0.14830566887003727 1105 40-41 0.004975091142902954 1105 42-43 0.09654492579888796 1105 44-45 0.22929649942197017 1105 46-47 0.26887213607255944 1105 48-49 0.04779842162469805 1105 50-51 -0.25326901367454013 1105 52-53 0.15226704587220752 1105 54-55 0.06486219616437694 1105 56-57 0.12031371201450725 1105 58-59 0.2248944624681073 1105 60-61 0.4828085785904861 1105 62-63 0.4149501836079068 1105 64-65 0.41926223609146973 1105 66-67 0.3194320096690788 1105 68-69 0.18975672985752112 1105 70-71 0.24045734059708934 1105 72-73 0.4939401701962538 1105 74-75 0.49051938365937175 1105 76-77 0.553940663307106 1105 78-79 0.3979707673774122 1105 80-81 0.5132797494299304 1105 82-83 0.5329739430375433 1105 84-85 0.6003776470794477 1105 86-87 0.549104725309288 1105 88-89 0.5739558425850753 1105 90-91 0.8662344850136598 1105 92-93 0.9621905905014536 1105 94-95 0.821786898427586 1105 96-97 0.7279315000285287 1105 98-99 0.824008458828839 1105 100-101 1.2146648726885871 1106 1 -0.172561950370838 1106 2 -0.12283780792959931 1106 3 -0.1463816115332648 1106 4 0.1069394190552444 1106 5 -0.03242799002185848 1106 6 -0.12100271737549662 1106 7 -0.5234826446071352 1106 8 -0.3068982275786851 1106 9 -0.4410359268790529 1106 10-11 -0.7391602115551734 1106 12-13 -0.4209892329063152 1106 14-15 -0.4990524642171934 1106 16-17 -0.24735885009528147 1106 18-19 -0.49907983330934513 1106 20-21 -0.27373912449478155 1106 22-23 -0.2757521958995923 1106 24-25 -0.21514162494412403 1106 26-27 -0.5580094113562382 1106 28-29 -0.17471814664124707 1106 30-31 -0.568615420381569 1106 32-33 -0.35132085230193155 1106 34-35 -0.1291304842779155 1106 36-37 -0.41635309868183157 1106 38-39 -0.39388027301442463 1106 40-41 -0.2661100529864129 1106 42-43 -0.2434976288728521 1106 44-45 -0.2761698046989878 1106 46-47 -0.3037890962736327 1106 48-49 -0.22926003017373375 1106 50-51 -0.40863096028986945 1106 52-53 -0.41827283029022055 1106 54-55 -0.5524298425174763 1106 56-57 -0.4308603099237587 1106 58-59 -0.4661568814246593 1106 60-61 -0.6156614244242888 1106 62-63 -0.4002228179458598 1106 64-65 -0.3150531206952465 1106 66-67 -0.22927337749727172 1106 68-69 -0.7747370782638114 1106 70-71 -0.5175767379261345 1106 72-73 -0.47579992163834817 1106 74-75 -0.2625690187431715 1106 76-77 -0.16518632818195655 1106 78-79 -0.18205839474777363 1106 80-81 -0.40662470547603036 1106 82-83 -0.20756257049414373 1106 84-85 -0.3647196952827443 1106 86-87 -0.41500440945103634 1106 88-89 0.005815899170034555 1106 90-91 0.24081995381076382 1106 92-93 0.12424446216491347 1106 94-95 0.0509681558195787 1106 96-97 0.36215757317154385 1106 98-99 0.6171736219744055 1106 100-101 0.6726679338420887 1107 1 0.044356576990185914 1107 2 -0.13707449367799285 1107 3 -0.06418594697372626 1107 4 -0.13662931585346172 1107 5 -0.002374271798359473 1107 6 -0.011408724049580599 1107 7 0.09949885244921575 1107 8 0.020989126934459534 1107 9 0.03698843623443082 1107 10-11 -0.05101586417732307 1107 12-13 -0.0011022989146098894 1107 14-15 0.12556228387689572 1107 16-17 0.08507522299404968 1107 18-19 0.07032350101876972 1107 20-21 0.11369813641842796 1107 22-23 0.2278585233277326 1107 24-25 -0.015780885368982922 1107 26-27 0.07645333858272352 1107 28-29 0.05938478615671272 1107 30-31 0.1500516622848096 1107 32-33 0.3628751378225914 1107 34-35 0.37748526764155343 1107 36-37 0.2286226874219679 1107 38-39 0.25608038788249843 1107 40-41 0.37318530228638735 1107 42-43 0.29699519513214057 1107 44-45 0.1306789296671056 1107 46-47 0.3379340920928513 1107 48-49 0.48833167063204286 1107 50-51 0.3393651193650271 1107 52-53 0.31310213918806085 1107 54-55 0.15805470671066502 1107 56-57 0.28265131545946076 1107 58-59 0.25950510072352273 1107 60-61 0.47484225174707007 1107 62-63 0.6397925107391345 1107 64-65 0.5644155979352021 1107 66-67 0.6415390428071319 1107 68-69 0.3971968456082351 1107 70-71 0.5181692806853277 1107 72-73 0.7176965785379963 1107 74-75 0.8200044368578965 1107 76-77 0.6385800518066453 1107 78-79 0.7304544921997866 1107 80-81 0.9674169125819709 1107 82-83 1.0450711146302467 1107 84-85 1.0166739263564786 1107 86-87 1.2521772829443059 1107 88-89 1.1288926918122648 1107 90-91 1.1417939357419051 1107 92-93 1.1750983920056548 1107 94-95 1.203780901688969 1107 96-97 1.3484760435586942 1107 98-99 1.4119476256098835 1107 100-101 1.819756840215021 1108 1 -0.016198511319316822 1108 2 0.13205923214202642 1108 3 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0.07514897470117532 2316 96-97 0.3195814956563403 2316 98-99 0.395529718658743 2316 100-101 0.17878258924688595 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5775.0 3 1343.0 4 964.0 5 1077.0 6 1181.0 7 1367.0 8 1683.0 9 2048.0 10 2765.0 11 3475.0 12 4698.0 13 6245.0 14 7162.0 15 9211.0 16 11741.0 17 15655.0 18 13608.0 19 8975.0 20 8801.0 21 9119.0 22 10026.0 23 10850.0 24 12040.0 25 13910.0 26 16006.0 27 18810.0 28 22391.0 29 26885.0 30 33291.0 31 41452.0 32 52432.0 33 68813.0 34 93012.0 35 132536.0 36 193122.0 37 272129.0 38 220570.0 39 27439.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.245666902560416 19.240301286587698 14.572432915298677 21.94159889555321 2 14.865076442668723 20.7631799454333 40.713658201386494 23.658085410511482 3 19.429888456437126 25.521370123140034 30.0891478767585 24.95959354366434 4 12.066626076367747 16.546127975413878 38.43575135542605 32.95149459279232 5 12.814760586248386 38.34838578739073 34.35692437047882 14.479929255882054 6 30.640574813482885 36.807131515410774 17.880411533971856 14.671882137134487 7 27.591347251878783 33.75920360494919 20.95141076626922 17.6980383769028 8 26.156197281224557 34.3975623671949 20.055300568157516 19.390939783423025 9 26.361208493906208 16.906402326748033 20.943414554934044 35.788974624411715 10-11 24.411837101198568 27.257574367955307 27.4686637566806 20.86192477416553 12-13 25.800375350690434 24.75393213743621 28.11051855600552 21.335173955867837 14-15 22.537580515436527 25.844426651801072 27.27765669678569 24.340336135976713 16-17 21.120609184972945 29.421283700872664 27.603059700112848 21.855047414041547 18-19 21.01709991576182 27.835607957535835 30.456633285535535 20.690658841166805 20-21 22.213040154751663 26.519780166806754 30.294008592240452 20.973171086201127 22-23 21.587873212835294 26.6831071140817 30.349551602744278 21.379468070338728 24-25 21.43916190716424 26.733272518979117 30.583314034380045 21.244251539476597 26-27 21.253840641773643 26.76888805605262 30.725641623223666 21.251629678950078 28-29 20.944848665875945 27.346099416239166 30.569398810438674 21.139653107446215 30-31 21.155992548531874 26.864761073437705 30.497841154970313 21.48140522306011 32-33 20.995986806594964 27.003198302358655 30.424544460317744 21.57627043072864 34-35 21.579770492302494 26.898383353405166 30.302099820694846 21.219746333597495 36-37 21.503050485507973 26.640979257858355 30.543310080320573 21.3126601763131 38-39 21.15163108835633 27.07919094712074 30.449700950824877 21.319477013698055 40-41 21.280405860888703 27.253502137597803 30.196087811850802 21.270004189662693 42-43 21.09490614639705 27.198715781011522 29.85573558252879 21.850642490062636 44-45 21.292124119200718 27.169109837183996 29.79916435085664 21.73960169275865 46-47 21.661099506314805 27.50288387848485 29.31880288772124 21.517213727479103 48-49 21.560195290875296 27.596020370170976 29.127009763456158 21.71677457549757 50-51 21.2943979970395 27.937108026379853 29.22383467890021 21.544659297680433 52-53 21.574634637364166 28.108918387356912 28.617075590468954 21.699371384809965 54-55 21.36851725836852 27.76170331245969 28.842823390709565 22.026956038462224 56-57 21.65896780249831 27.9706349796036 28.551085240833395 21.819311977064697 58-59 21.970091748305677 27.67158324714641 28.591349321219088 21.766975683328827 60-61 21.603662582353927 27.706541169444804 29.243548753561676 21.44624749463959 62-63 21.332810028890464 27.794568940649157 29.363141392221753 21.509479638238624 64-65 21.306796666042178 27.96895060630098 28.963187820856838 21.7610649068 66-67 21.357421988241708 27.850719528740562 28.640729907116437 22.151128575901296 68-69 21.33092660929135 27.826472345805804 28.945700493315613 21.89690055158723 70-71 21.540645917868506 27.80000239177837 28.687497123524118 21.971854566829006 72-73 21.462960661047763 27.502910983167578 28.990022023087096 22.04410633269756 74-75 21.22084310771529 28.27021254003223 28.54991720843289 21.95902714381959 76-77 21.341526180576096 27.77528894610785 28.642852172097637 22.240332701218417 78-79 21.461360649641815 27.730827469880566 28.564045430855955 22.243766449621663 80-81 21.48227073729379 27.795765562030674 28.43687483691398 22.28508886376156 82-83 21.39826469899681 27.676101305860755 28.883752517752008 22.041881477390422 84-85 21.312280409292615 27.801673917652238 28.57927190561699 22.306773767438152 86-87 21.237481285126066 27.87861127752595 28.546094856821018 22.337812580526965 88-89 21.26747062181189 27.654329488724393 28.373648875157425 22.704551014306293 90-91 21.194811652940217 27.42366970408792 28.6624388507696 22.71907979220226 92-93 21.3326036889323 27.35753360864121 28.78294951500996 22.526913187416532 94-95 21.13498900127201 27.30942484081742 28.844390648725632 22.711195509184932 96-97 20.803027908777107 27.31112538291028 29.122492146080724 22.763354562231893 98-99 20.948442966272847 27.39459449271538 28.62096062145066 23.036001919561112 100-101 20.939028995055033 26.98177327370094 29.15765259420476 22.921545137039267 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2048.0 1 1225.5 2 416.5 3 437.5 4 364.5 5 351.5 6 578.0 7 1048.5 8 1658.0 9 2056.5 10 1981.5 11 1838.0 12 1947.5 13 2079.5 14 2207.0 15 2349.0 16 2439.5 17 2549.0 18 2723.5 19 2924.0 20 3186.0 21 3608.0 22 4292.5 23 5241.0 24 6168.0 25 7353.5 26 9419.0 27 11788.0 28 13769.5 29 15986.0 30 18467.0 31 21319.5 32 24393.0 33 27043.0 34 30302.5 35 33062.5 36 36102.5 37 39831.5 38 41629.5 39 42072.0 40 42978.0 41 44913.5 42 46362.5 43 47729.0 44 50166.0 45 51907.5 46 52752.0 47 56278.0 48 63046.0 49 61462.0 50 52831.0 51 47131.5 52 43239.0 53 41424.0 54 39889.5 55 36011.5 56 31023.0 57 26977.0 58 24121.5 59 21019.0 60 17403.5 61 14178.0 62 11310.0 63 8464.0 64 6071.5 65 4495.5 66 3278.5 67 2404.0 68 1745.5 69 1248.5 70 902.5 71 657.0 72 515.0 73 431.0 74 310.0 75 214.5 76 175.0 77 115.5 78 66.0 79 44.0 80 23.0 81 16.5 82 11.5 83 7.5 84 5.5 85 3.5 86 2.5 87 4.5 88 4.0 89 1.0 90 0.5 91 0.5 92 1.0 93 1.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25010668228929345 2 0.21799350503757386 3 0.20830169028142426 4 0.2148111181027188 5 0.216257657618562 6 0.24446517817750488 7 0.24555008281438728 8 0.23426707459081011 9 0.22956582116431964 10-11 0.24131895473054585 12-13 0.2434887640043107 14-15 0.22699821352369795 16-17 0.24045103102103993 18-19 0.23057839882540992 20-21 0.22490073122572526 22-23 0.2231648838067134 24-25 0.21708941784017183 26-27 0.22569632795943906 28-29 0.2370878266467044 30-31 0.23755795198935345 32-33 0.23755795198935345 34-35 0.24569473676597162 36-37 0.22244161404879176 38-39 0.22211614265772706 40-41 0.21871677479549548 42-43 0.22381582658884286 44-45 0.24034254055735169 46-47 0.24526077491121862 48-49 0.24855165230976195 50-51 0.2496365569466444 52-53 0.26804377228574944 54-55 0.2486963062613463 56-57 0.22258626800037612 58-59 0.20663816983820457 60-61 0.20385358127020634 62-63 0.21090546140994207 64-65 0.20313031151228475 66-67 0.20381741778231027 68-69 0.20718062215664576 70-71 0.2085548346966968 72-73 0.21130325977679895 74-75 0.21267747231685 76-77 0.22522620261679 78-79 0.21835513991653469 80-81 0.2148111181027188 82-83 0.2102545186278126 84-85 0.21065231699466952 86-87 0.21882526525918372 88-89 0.2149919355421992 90-91 0.223888153564635 92-93 0.21282212626843436 94-95 0.2101098646762283 96-97 0.22016331431133873 98-99 0.22623878027788025 100-101 0.2041790526612711 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1382610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.28307443197133 #Duplication Level Percentage of deduplicated Percentage of total 1 79.55482473388007 47.958094208549255 2 12.01599766032996 14.487225626641287 3 3.6710123167947586 6.638997262020658 4 1.5561232793500017 3.7523158189751786 5 0.8553779160140688 2.578240528927031 6 0.5030914455808096 1.8196739436021598 7 0.33518714183268267 1.414427799381754 8 0.2337425531121358 1.1272575781742316 9 0.1832768162430227 0.9943640959709581 >10 0.9987314612365884 10.893339226478815 >50 0.0633933910899039 2.6329958357405685 >100 0.026353301818738756 2.702370212230646 >500 0.001443991358650457 0.6148507249768768 >1k 0.0012033261322087143 1.5010017934428426 >5k 2.406652264417428E-4 0.8848453448876761 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6950 0.5026724817555204 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5248 0.379571968957262 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4078 0.2949494072804334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3378 0.24432052422592052 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2717 0.19651239322730196 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2011 0.14544954831803616 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1964 0.14205018045580461 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1629 0.11782064356543061 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.23269757921612E-5 0.0 3 0.0 0.0 0.0 1.446539515843224E-4 0.0 4 0.0 0.0 0.0 1.446539515843224E-4 0.0 5 0.0 0.0 0.0 1.446539515843224E-4 0.0 6 7.23269757921612E-5 0.0 0.0 1.446539515843224E-4 0.0 7 7.23269757921612E-5 0.0 0.0 2.169809273764836E-4 0.0 8 7.23269757921612E-5 0.0 0.0 2.893079031686448E-4 0.0 9 7.23269757921612E-5 0.0 0.0 3.61634878960806E-4 0.0 10-11 7.23269757921612E-5 0.0 0.0 3.61634878960806E-4 0.0 12-13 2.169809273764836E-4 0.0 0.0 3.61634878960806E-4 0.0 14-15 2.169809273764836E-4 0.0 0.0 3.61634878960806E-4 0.0 16-17 2.169809273764836E-4 0.0 0.0 4.7012534264904786E-4 0.0 18-19 2.169809273764836E-4 0.0 0.0 5.062888305451285E-4 0.0 20-21 2.169809273764836E-4 0.0 0.0 5.424523184412091E-4 0.0 22-23 2.169809273764836E-4 0.0 0.0 5.786158063372896E-4 0.0 24-25 2.169809273764836E-4 0.0 0.0 8.679237095059344E-4 0.0 26-27 2.169809273764836E-4 0.0 0.0 0.0011933951005706597 0.0 28-29 2.169809273764836E-4 0.0 0.0 0.002639934616413884 0.0 30-31 2.169809273764836E-4 0.0 0.0 0.006364773869710186 0.0 32-33 2.169809273764836E-4 0.0 0.0 0.012187095420979163 0.0 34-35 2.169809273764836E-4 0.0 0.0 0.017647782093287333 0.0 36-37 2.893079031686448E-4 0.0 0.0 0.023253122717179824 0.0 38-39 2.893079031686448E-4 0.0 0.0 0.029907204490058657 0.0 40-41 2.893079031686448E-4 0.0 0.0 0.038839586000390565 0.0 42-43 2.893079031686448E-4 0.0 0.0 0.04997794027238339 0.0 44-45 2.893079031686448E-4 0.0 0.0 0.06357541172130969 0.0 46-47 2.893079031686448E-4 0.0 0.0 0.08172948264514215 0.0 48-49 2.893079031686448E-4 0.0 0.0 0.100498332863208 0.0 50-51 2.893079031686448E-4 0.0 0.0 0.12805491064002142 0.0 52-53 2.893079031686448E-4 0.0 0.0 0.1821193250446619 0.0 54-55 2.893079031686448E-4 0.0 0.0 0.25289127085729163 0.0 56-57 2.893079031686448E-4 0.0 0.0 0.319468252073976 0.0 58-59 2.893079031686448E-4 0.0 0.0 0.4221002307230528 0.0 60-61 2.893079031686448E-4 0.0 0.0 0.5512762094878527 0.0 62-63 2.893079031686448E-4 0.0 0.0 0.6404553706395875 0.0 64-65 2.893079031686448E-4 0.0 0.0 0.7151691366328901 7.23269757921612E-5 66-67 2.893079031686448E-4 0.0 0.0 0.8922617368599967 7.23269757921612E-5 68-69 2.893079031686448E-4 0.0 0.0 1.0754298030536449 7.23269757921612E-5 70-71 2.893079031686448E-4 0.0 0.0 1.1829438525686924 7.23269757921612E-5 72-73 2.893079031686448E-4 0.0 0.0 1.3758398970063865 7.23269757921612E-5 74-75 2.893079031686448E-4 0.0 0.0 1.643883669292136 7.23269757921612E-5 76-77 2.893079031686448E-4 0.0 0.0 1.9079494579093166 7.23269757921612E-5 78-79 2.893079031686448E-4 0.0 0.0 2.1974381785174417 7.23269757921612E-5 80-81 2.893079031686448E-4 0.0 0.0 2.5350605015152503 7.23269757921612E-5 82-83 2.893079031686448E-4 0.0 0.0 2.8434265628051296 7.23269757921612E-5 84-85 2.893079031686448E-4 0.0 0.0 3.160327207238484 1.0849046368824179E-4 86-87 2.893079031686448E-4 0.0 0.0 3.607850369952481 1.446539515843224E-4 88-89 2.893079031686448E-4 0.0 0.0 4.190118688567274 1.446539515843224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7370 0.0 25.40397 1 TACATGG 7345 0.0 25.292677 2 GTATCAA 12335 0.0 24.424347 1 ACATGGG 7410 0.0 23.724298 3 TACCTGG 1035 0.0 22.953003 2 GAGTACT 6180 0.0 22.443674 12-13 GTCGGGA 325 1.8189894E-12 21.928947 2 CATGGGA 4910 0.0 20.805714 4 ATCAACG 14255 0.0 20.76494 3 TCAACGC 14315 0.0 20.745039 4 GTACTTT 6715 0.0 20.724392 14-15 CAACGCA 14345 0.0 20.667032 5 TATCAAC 14405 0.0 20.647663 2 AGTACTT 6485 0.0 20.61902 12-13 AACGCAG 14595 0.0 20.343363 6 CATGGGG 2555 0.0 20.08438 4 GTATACG 120 0.00475261 19.799837 1 GGTATCA 9235 0.0 19.501822 1 ATGGGAG 1995 0.0 19.290176 5 AGAGTAC 10905 0.0 19.27538 10-11 >>END_MODULE