FastQCFastQC Report
Thu 26 May 2016
SRR937428_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937428_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382610
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89980.6507981281778665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75040.5427416263443776No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52690.3810908354488974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.3651065737988297No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22650.16382060016924513No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18700.13525144473134146No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18180.1314904419901491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG75450.026.1407951
TACATGG75500.025.9834842
ACATGGG77100.024.7665753
GAGTACT74000.023.52707512-13
TACCTGG13000.023.3846512
CATGGGA50750.022.650284
TAGACCG1050.002196426522.6190815
GTACCTG14950.022.2529071
GTACTTT78650.021.86192514-15
GTATCAA147900.021.4332281
AGTACTT75650.021.1615112-13
TACACCG1805.1699353E-621.1111435
ATACCGT1150.00372440220.6514596
GGTATCA116400.019.9249291
ATGGGAG19600.019.872485
CGTACAC1456.1468413E-419.6552013
AGAGTAC121300.019.46351410-11
CATGGGG26650.019.249564
ACTTTTT91750.018.71461116-17
ATGGGAT20400.018.627485
Produced by FastQC (version 0.11.5)