##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937428_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1382610 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21691294001924 33.0 31.0 34.0 31.0 34.0 2 32.41061615350677 34.0 31.0 34.0 31.0 34.0 3 32.45700812231938 34.0 31.0 34.0 31.0 34.0 4 36.1063409059677 37.0 35.0 37.0 35.0 37.0 5 36.04400445534171 37.0 35.0 37.0 35.0 37.0 6 36.09918125863403 37.0 35.0 37.0 35.0 37.0 7 36.042446532283144 37.0 35.0 37.0 35.0 37.0 8 36.01941689992116 37.0 35.0 37.0 35.0 37.0 9 37.72242859519315 39.0 37.0 39.0 35.0 39.0 10-11 37.64827355508784 39.0 37.0 39.0 35.0 39.0 12-13 37.55714482030363 39.0 37.0 39.0 35.0 39.0 14-15 38.73241369583614 40.0 38.0 41.0 34.5 41.0 16-17 38.71765682296527 40.0 38.0 41.0 34.5 41.0 18-19 38.7404087197402 40.0 38.0 41.0 35.0 41.0 20-21 38.65906907949458 40.0 38.0 41.0 34.0 41.0 22-23 38.51320509760525 40.0 38.0 41.0 34.0 41.0 24-25 38.45196512393227 40.0 38.0 41.0 34.0 41.0 26-27 38.39386956553186 40.0 38.0 41.0 34.0 41.0 28-29 38.27184093851484 40.0 38.0 41.0 34.0 41.0 30-31 38.31944836215563 40.0 38.0 41.0 34.0 41.0 32-33 38.22840714301213 40.0 38.0 41.0 34.0 41.0 34-35 38.08781145803951 40.0 37.0 41.0 34.0 41.0 36-37 37.90895299469844 40.0 37.0 41.0 33.0 41.0 38-39 37.78349389921959 40.0 37.0 41.0 33.0 41.0 40-41 37.59533599496604 40.0 37.0 41.0 33.0 41.0 42-43 37.353920122087935 40.0 37.0 41.0 33.0 41.0 44-45 37.025900290031174 40.0 36.0 41.0 31.5 41.0 46-47 36.60169208959866 40.0 36.0 41.0 30.5 41.0 48-49 36.209446626308214 39.0 36.0 41.0 30.0 41.0 50-51 35.94340377980775 39.0 35.5 41.0 29.0 41.0 52-53 35.70451754290798 39.0 35.0 40.5 28.0 41.0 54-55 35.482307013546844 39.0 35.0 40.0 28.0 41.0 56-57 35.2113719704038 38.5 35.0 40.0 27.0 41.0 58-59 34.95087081678854 38.0 34.5 40.0 26.5 41.0 60-61 34.61511814611495 38.0 34.0 40.0 26.0 41.0 62-63 34.267629700349346 37.5 34.0 40.0 25.5 41.0 64-65 33.87739601189055 37.0 33.5 40.0 24.5 41.0 66-67 33.45706996188368 36.5 33.0 39.0 23.0 41.0 68-69 33.02687815074388 36.0 33.0 39.0 22.0 40.0 70-71 32.614914545678104 35.5 32.0 38.5 21.5 40.0 72-73 32.14845364925756 35.0 32.0 38.0 20.5 40.0 74-75 31.679834877514267 35.0 31.0 37.0 18.5 39.0 76-77 30.41974490275638 34.0 29.5 36.0 18.0 38.5 78-79 31.0152566522736 34.0 31.0 36.0 18.0 38.5 80-81 30.818697969781788 34.0 31.0 36.0 17.0 37.5 82-83 30.474217241304487 34.0 31.0 35.0 12.0 37.0 84-85 30.120612464831005 34.0 30.5 35.0 7.5 37.0 86-87 29.71938905403548 34.0 30.0 35.0 4.5 36.0 88-89 29.258574362980163 34.0 29.5 35.0 2.0 36.0 90-91 28.758089048972593 34.0 29.0 35.0 2.0 35.0 92-93 28.426064110631344 34.0 29.0 35.0 2.0 35.0 94-95 27.9997508335684 33.5 29.0 35.0 2.0 35.0 96-97 27.52909967380534 33.0 27.0 35.0 2.0 35.0 98-99 26.804680278603513 33.0 26.0 35.0 2.0 35.0 100-101 24.347071842385056 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0053651394196663205 1101 2 -0.0048092550936402745 1101 3 0.011842523283370099 1101 4 -0.013673469076024958 1101 5 0.09308229382008193 1101 6 -0.10312342438343336 1101 7 -0.01461086097692288 1101 8 -0.038652011992866164 1101 9 -0.008777739380860794 1101 10-11 -0.02115907127367933 1101 12-13 -0.0015335692010580715 1101 14-15 -0.12024641141348269 1101 16-17 -0.02645583065080359 1101 18-19 0.04472708897186095 1101 20-21 -0.0470309413513732 1101 22-23 -0.011168789777286747 1101 24-25 -0.027678986977043962 1101 26-27 -0.06843266626590605 1101 28-29 -0.2051788160437411 1101 30-31 -0.12379244048974414 1101 32-33 0.011331541848079496 1101 34-35 -0.02073134689587164 1101 36-37 -0.0208389431362761 1101 38-39 0.111674492036137 1101 40-41 0.08350152021353807 1101 42-43 0.038605688238192215 1101 44-45 0.09739010061403519 1101 46-47 0.027767510933550454 1101 48-49 0.0938660441232031 1101 50-51 0.22121520119078042 1101 52-53 0.22653681044354101 1101 54-55 0.24152569282313152 1101 56-57 0.21876096592372107 1101 58-59 0.1651575501659721 1101 60-61 0.22789646506012673 1101 62-63 0.14738223635671233 1101 64-65 0.1214753507302575 1101 66-67 0.08766645192193323 1101 68-69 -0.10882739496880589 1101 70-71 -0.08096860509013482 1101 72-73 0.0012940387129418696 1101 74-75 0.0822988131315121 1101 76-77 0.01493368602704237 1101 78-79 -0.08821896755350522 1101 80-81 -0.08981563160385875 1101 82-83 0.12387617427909703 1101 84-85 -0.01133701959219735 1101 86-87 -0.07133846854078385 1101 88-89 0.005616600930395066 1101 90-91 0.10560290384049509 1101 92-93 0.21785915888456842 1101 94-95 0.2568962082148296 1101 96-97 0.12462484501613957 1101 98-99 0.1943391170001938 1101 100-101 0.9822355804935299 1102 1 -0.08712288513778788 1102 2 -0.10227287106764749 1102 3 -0.013308777564617458 1102 4 0.011938735715517623 1102 5 -0.06374905701773059 1102 6 -0.1068175453779574 1102 7 -0.1085694712516343 1102 8 -0.09166698861525902 1102 9 -0.2270553709684222 1102 10-11 -0.12833095020820195 1102 12-13 -0.16206214128413166 1102 14-15 -0.01392417341825336 1102 16-17 -0.10622734906061027 1102 18-19 -0.17546839217155252 1102 20-21 0.049011209657706445 1102 22-23 -0.09441034994746644 1102 24-25 -0.04851198301457771 1102 26-27 -0.09590002665386521 1102 28-29 -0.1404197451406617 1102 30-31 -0.09195062630568174 1102 32-33 -0.11407583971114832 1102 34-35 -0.0447844700367952 1102 36-37 -0.12333288817433896 1102 38-39 0.057785244704675165 1102 40-41 0.07853853548502343 1102 42-43 0.049617434399301885 1102 44-45 -0.003973630792643235 1102 46-47 0.007468274731991187 1102 48-49 0.1451080217938312 1102 50-51 0.15242408196236568 1102 52-53 0.1728967888244597 1102 54-55 0.08929080818174384 1102 56-57 -0.032473019989424756 1102 58-59 0.14898440322890139 1102 60-61 0.06494849174570305 1102 62-63 0.09088481697098416 1102 64-65 -0.07536215238290822 1102 66-67 0.011833457606762465 1102 68-69 -0.13592909800542685 1102 70-71 -0.028338059559679607 1102 72-73 -0.04733442758268325 1102 74-75 0.07384374041512842 1102 76-77 0.1280976607000639 1102 78-79 0.22367125187879466 1102 80-81 -0.11797917051940843 1102 82-83 -0.03726848652074466 1102 84-85 -0.08636325976999615 1102 86-87 -0.09729135644156983 1102 88-89 -0.01798302442152533 1102 90-91 -0.1321880503856825 1102 92-93 -0.1955008894242276 1102 94-95 -0.22004412677070917 1102 96-97 0.05882753458699952 1102 98-99 0.3335817119651807 1102 100-101 1.0260357507317437 1103 1 -0.023177896237463358 1103 2 0.09486828438202366 1103 3 -0.04078725501332059 1103 4 -0.01908622502951829 1103 5 -0.11959940083710308 1103 6 -0.046148315858218325 1103 7 -0.04633004302054644 1103 8 -0.06386364246765908 1103 9 0.016975166689270793 1103 10-11 -0.035851660587752576 1103 12-13 -0.044757468589345706 1103 14-15 -0.21314990301920744 1103 16-17 -0.2398702100709471 1103 18-19 -0.15521824327340283 1103 20-21 -0.24151616053017477 1103 22-23 -0.0704377472077411 1103 24-25 -0.05403475089589449 1103 26-27 -0.09844483146192573 1103 28-29 -0.2688917793601675 1103 30-31 -0.2234141597942383 1103 32-33 -0.12949356671315115 1103 34-35 -0.21600381055333884 1103 36-37 -0.17742352654133242 1103 38-39 -0.16197489390597042 1103 40-41 -0.28134830191819304 1103 42-43 -0.22671046640744663 1103 44-45 -0.17549076761650895 1103 46-47 -0.011929093831547277 1103 48-49 -0.2262169010498596 1103 50-51 -0.05937677352520865 1103 52-53 0.003098748865610901 1103 54-55 -0.0768253865228985 1103 56-57 -0.24511311961583004 1103 58-59 -0.23704082824004757 1103 60-61 -0.42093325604790266 1103 62-63 -0.22798734719599878 1103 64-65 -0.40938131963614666 1103 66-67 -0.1631218729471442 1103 68-69 -0.3150535768618923 1103 70-71 -0.15366969635647365 1103 72-73 -0.03297252385210925 1103 74-75 0.11895988097498744 1103 76-77 0.07131331937134888 1103 78-79 -0.12465567432186475 1103 80-81 -0.059638166675132 1103 82-83 -0.10337926674706921 1103 84-85 -0.15953993419741863 1103 86-87 0.008453405050655505 1103 88-89 0.020595133572953728 1103 90-91 -0.0011576751623358916 1103 92-93 -0.1633659606246205 1103 94-95 -0.05900400548639695 1103 96-97 0.22885568987424065 1103 98-99 0.09366617782689346 1103 100-101 0.6483623520637032 1104 1 -0.05618164894334399 1104 2 -0.04486693672673425 1104 3 -0.02666404256336108 1104 4 0.007745204091143876 1104 5 -0.048574636321482956 1104 6 -0.015853364877415288 1104 7 0.0014588693633825756 1104 8 -0.046597016476198405 1104 9 0.0017904984568914983 1104 10-11 0.03216926764279293 1104 12-13 -0.03454116563148091 1104 14-15 -0.04658743174955049 1104 16-17 -0.06732555788576633 1104 18-19 -0.09003482872895319 1104 20-21 -0.08729415747117741 1104 22-23 -0.061823239530994556 1104 24-25 -0.09428147668091214 1104 26-27 -0.14708541584765555 1104 28-29 -0.10562734314363098 1104 30-31 -0.1884768024012331 1104 32-33 -0.08540804848034611 1104 34-35 -0.05443686779673129 1104 36-37 -0.12526676540230852 1104 38-39 -0.1153017637815239 1104 40-41 -0.06158641904607265 1104 42-43 0.07547554744998308 1104 44-45 0.2524593690354777 1104 46-47 0.18243599681009215 1104 48-49 -0.0063697673983682535 1104 50-51 -0.1667655923004432 1104 52-53 0.0990439041975506 1104 54-55 0.08189526673209002 1104 56-57 0.04412438738393121 1104 58-59 -0.13644616226430628 1104 60-61 -0.02903542325643116 1104 62-63 -0.008991467650339757 1104 64-65 -0.12677498600471182 1104 66-67 -0.005458914410780835 1104 68-69 0.10916725608836941 1104 70-71 0.12330841425222872 1104 72-73 0.08551310470523532 1104 74-75 0.18736109454490446 1104 76-77 0.08522298697688768 1104 78-79 -0.20149253609023177 1104 80-81 0.0010837904153113698 1104 82-83 0.045986367159336794 1104 84-85 0.030795803430770263 1104 86-87 -0.10098143242959523 1104 88-89 -0.0904314696849653 1104 90-91 -0.1862224908284169 1104 92-93 -0.053926526288613275 1104 94-95 0.04926406642514536 1104 96-97 0.04530658291794154 1104 98-99 0.3638747914987803 1104 100-101 0.7794124410755465 1105 1 0.0554014427438716 1105 2 -0.007955150025075852 1105 3 0.042533917476973215 1105 4 -0.027462434660364465 1105 5 -0.04986673943884057 1105 6 -0.011368155549959624 1105 7 0.06691754667051697 1105 8 -0.04213303662490375 1105 9 -0.07487396484747677 1105 10-11 -0.08014189857917842 1105 12-13 -0.04631122145535471 1105 14-15 -0.024874985004181838 1105 16-17 -0.08071170758252322 1105 18-19 -0.12062698636746205 1105 20-21 -0.03631760156769559 1105 22-23 -0.08414868740057102 1105 24-25 -0.26717240059736724 1105 26-27 -0.11103197546629673 1105 28-29 -0.2761938806643158 1105 30-31 -0.08903624734693238 1105 32-33 -0.013843725580017008 1105 34-35 0.03817310330222767 1105 36-37 0.007090333686271322 1105 38-39 -0.1849071850177566 1105 40-41 -0.2400283684909752 1105 42-43 -0.1720314365651845 1105 44-45 -0.24873671895105076 1105 46-47 -0.2550197952182387 1105 48-49 -0.060053081512592144 1105 50-51 -0.32068411936078434 1105 52-53 -0.3982341392167328 1105 54-55 -0.26121480327692836 1105 56-57 -0.37636181586900364 1105 58-59 -0.3332938262981955 1105 60-61 -0.4596707635794104 1105 62-63 -0.3290289569752858 1105 64-65 -0.3371253206102054 1105 66-67 -0.22542306554534974 1105 68-69 -0.13267025157112755 1105 70-71 -0.24666321544780345 1105 72-73 -0.06341842540926734 1105 74-75 0.06456666824737312 1105 76-77 0.2484028730131982 1105 78-79 -0.09390806448707067 1105 80-81 -0.39637011990945226 1105 82-83 -0.4191420470470426 1105 84-85 -0.2157445156868505 1105 86-87 -0.16086134114285144 1105 88-89 -0.18430194093108554 1105 90-91 -0.1354012960913984 1105 92-93 0.13250108361011215 1105 94-95 0.2606691200054492 1105 96-97 0.14702448177878935 1105 98-99 0.41260584780996723 1105 100-101 0.8869614498037564 1106 1 -0.12719810272121634 1106 2 -0.03294877262194973 1106 3 0.02755223801685247 1106 4 0.0072364359595127326 1106 5 -0.009875783755994405 1106 6 -0.100687744613289 1106 7 -0.15964201827463143 1106 8 -0.190989742174402 1106 9 -0.04159551583191501 1106 10-11 -0.22982737665251562 1106 12-13 -0.16429057026451233 1106 14-15 -0.16595038909962767 1106 16-17 -0.07813146825598238 1106 18-19 -0.05352800907101596 1106 20-21 -0.01938443675241075 1106 22-23 -0.2577720379722166 1106 24-25 -0.18221988326242666 1106 26-27 -0.30898193024471254 1106 28-29 -0.30599809438849235 1106 30-31 -0.1964276575646906 1106 32-33 -0.11603697248987288 1106 34-35 -0.10472102021067542 1106 36-37 -0.25675355908060027 1106 38-39 -0.18961167934765655 1106 40-41 -0.2083091945386073 1106 42-43 -0.29778165107783394 1106 44-45 -0.15131579874975642 1106 46-47 -0.17864865354712833 1106 48-49 -0.21877504992705354 1106 50-51 -0.01958546673213135 1106 52-53 0.012607446089731411 1106 54-55 -0.04263655833827329 1106 56-57 -0.298430819370779 1106 58-59 -0.22126430471938363 1106 60-61 -0.20337510717070018 1106 62-63 0.049968805135982564 1106 64-65 0.1441819310769077 1106 66-67 0.19179969134803798 1106 68-69 0.23657037364628053 1106 70-71 0.03532670825526196 1106 72-73 0.23214116272644958 1106 74-75 0.08154273559275182 1106 76-77 0.24093580360386113 1106 78-79 -0.013992536090231766 1106 80-81 0.06623858582556963 1106 82-83 -0.17641837412665495 1106 84-85 -0.1093752292602872 1106 86-87 0.044756281361269856 1106 88-89 0.1038178266995331 1106 90-91 -0.01260265969613883 1106 92-93 0.31453796819070945 1106 94-95 0.3881171731582036 1106 96-97 0.16934046471054032 1106 98-99 0.3165566490437044 1106 100-101 0.7475548961524012 1107 1 0.03826013000349349 1107 2 -0.04949154372263109 1107 3 0.10328611576719737 1107 4 0.05784144423429183 1107 5 0.03264978666099694 1107 6 0.006055520260957792 1107 7 -0.05630158581580247 1107 8 -0.03102474041092762 1107 9 -0.1143050304687776 1107 10-11 0.05111248878062469 1107 12-13 0.007047840978330555 1107 14-15 0.05213576353609284 1107 16-17 -0.016626476259453682 1107 18-19 0.08362641725634745 1107 20-21 0.12382821144748846 1107 22-23 0.16626110183081977 1107 24-25 0.07058099305000809 1107 26-27 0.008883448631543445 1107 28-29 0.0023814736952942894 1107 30-31 0.07351116334743324 1107 32-33 -0.01283761819377105 1107 34-35 0.03901626240687506 1107 36-37 0.11772858910573092 1107 38-39 0.08334438294377122 1107 40-41 0.013699337977193693 1107 42-43 -0.14072299511864372 1107 44-45 0.001133738676649898 1107 46-47 -0.20090373371751014 1107 48-49 -0.2617428461233686 1107 50-51 -0.38677733303344297 1107 52-53 -0.24181074214109088 1107 54-55 -0.2367801189340497 1107 56-57 -0.3343728552371843 1107 58-59 -0.5460879570159705 1107 60-61 -0.3385191696791807 1107 62-63 -0.16353366373186162 1107 64-65 -0.01754383467908127 1107 66-67 -0.131925518234425 1107 68-69 0.1912225617143264 1107 70-71 0.09999035892402475 1107 72-73 0.3089561875507769 1107 74-75 0.29103092029662037 1107 76-77 0.14464351406806486 1107 78-79 0.1339574218576196 1107 80-81 0.1822658790239764 1107 82-83 0.02314584356780358 1107 84-85 -0.01963459603637574 1107 86-87 -0.09859574935036264 1107 88-89 0.20281027087870385 1107 90-91 0.3802783191692676 1107 92-93 0.42564685611496245 1107 94-95 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0.558464276605271 2315 48-49 0.40462954913590465 2315 50-51 0.40142131877058773 2315 52-53 0.4664498716376855 2315 54-55 0.36212069837991834 2315 56-57 0.2622933714849509 2315 58-59 0.3663319578688018 2315 60-61 0.4820266376728597 2315 62-63 0.39034187315257185 2315 64-65 0.3028980708798059 2315 66-67 0.47078280837104103 2315 68-69 0.4408638197123125 2315 70-71 0.8296108646449412 2315 72-73 0.6181110587897791 2315 74-75 0.7415957809909912 2315 76-77 0.6008306552383083 2315 78-79 0.39508509329207087 2315 80-81 0.32541517045586943 2315 82-83 0.5273008622350233 2315 84-85 0.30594061973535247 2315 86-87 0.24895750382882298 2315 88-89 0.2615477798472554 2315 90-91 0.4769336642846902 2315 92-93 0.3686732204929335 2315 94-95 0.5594140927553823 2315 96-97 0.5931469432500371 2315 98-99 0.846869940012521 2315 100-101 0.8468682679010158 2316 1 -0.6526234179636781 2316 2 -0.56611287841163 2316 3 -0.2667337116042603 2316 4 -0.15737104599453744 2316 5 0.049544050311155274 2316 6 -0.10000334649426179 2316 7 0.040370711230863776 2316 8 0.020588117471568523 2316 9 0.016361173897621484 2316 10-11 0.054979958537835216 2316 12-13 -0.07001847334056777 2316 14-15 -0.1305193081399736 2316 16-17 -0.08938098153959118 2316 18-19 -0.10254783714781013 2316 20-21 -0.09898802497536963 2316 22-23 -0.017560653301529783 2316 24-25 0.10756618616579772 2316 26-27 0.013856300204260208 2316 28-29 0.17796460067246045 2316 30-31 0.022363338930951215 2316 32-33 -0.03207540062058456 2316 34-35 -0.19330602335561053 2316 36-37 -0.13864658630676985 2316 38-39 -0.05913312482045541 2316 40-41 -0.13605315176746302 2316 42-43 -0.029014616004310767 2316 44-45 -0.15395043199024627 2316 46-47 -0.03748293036145611 2316 48-49 -0.24680242585181134 2316 50-51 0.23549210837195034 2316 52-53 0.2642682721597609 2316 54-55 0.1511682908545282 2316 56-57 0.21016571520855365 2316 58-59 0.23737902951250334 2316 60-61 0.2489094646686354 2316 62-63 0.4596166471104013 2316 64-65 0.17134370062792925 2316 66-67 0.3072581781842345 2316 68-69 0.3873573566037223 2316 70-71 0.3901934977242192 2316 72-73 0.3490197382199085 2316 74-75 0.33585803795594416 2316 76-77 0.011210536284249173 2316 78-79 0.014895579044402751 2316 80-81 0.2234090413921095 2316 82-83 0.2534147195924561 2316 84-85 0.3291861868597614 2316 86-87 -0.03982192273014462 2316 88-89 -0.13084948393035134 2316 90-91 -0.17520786531509103 2316 92-93 0.032630713870165806 2316 94-95 0.17235767095674603 2316 96-97 0.43266863136222966 2316 98-99 0.5475421374074365 2316 100-101 -0.06527794297659995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 0.0 4 8.0 5 27.0 6 49.0 7 132.0 8 345.0 9 611.0 10 1146.0 11 1956.0 12 3142.0 13 4467.0 14 5760.0 15 8482.0 16 11895.0 17 17553.0 18 15413.0 19 8594.0 20 6997.0 21 6860.0 22 6897.0 23 7587.0 24 8345.0 25 10091.0 26 12231.0 27 15275.0 28 18720.0 29 23808.0 30 29869.0 31 37748.0 32 48684.0 33 64578.0 34 89253.0 35 128097.0 36 199569.0 37 308367.0 38 255714.0 39 24334.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.851777325796014 19.90431176125712 15.16788870632686 21.076022206620003 2 15.552541931564216 21.612891560165195 39.6374248703539 23.197141637916694 3 19.92499692610353 25.746884515517753 30.41833922798186 23.90977933039686 4 12.697579216120236 17.322310702222605 37.61089533563333 32.36921474602382 5 12.88591604000327 38.733620379766336 34.01174754283094 14.368716037399453 6 30.75227287521427 37.37720687684886 17.2531661133653 14.617354134571572 7 27.649156305827383 34.174134426917206 20.600313899074937 17.57639536818047 8 26.664279876465525 34.64527234722735 19.49197532203586 19.198472454271272 9 26.592314535552326 17.031411605586534 20.60704030782361 35.76923355103753 10-11 24.80176044177397 27.572358749681307 26.904960373786484 20.720920434758238 12-13 26.21874632695316 24.978309810764625 27.86745386653165 20.935489995750565 14-15 22.74766564685631 26.10320336175784 27.313739955591238 23.835391035794622 16-17 21.071451819385075 29.554068030753427 27.69226318339951 21.682216966461983 18-19 21.00784024417587 27.631617014197786 30.899096636072354 20.46144610555399 20-21 22.155054570703236 26.340110370965057 30.983429889846015 20.52140516848569 22-23 21.720932200539636 26.359730348521026 30.989306737055816 20.93003071388352 24-25 21.54949039723448 26.516196157013272 31.268016373540654 20.666297072211595 26-27 21.404207429180634 26.501806015035417 31.418766933651188 20.67521962213276 28-29 20.98106921709897 27.141439485940243 31.246822025470088 20.630669271490703 30-31 21.208365669682475 26.66159410908265 31.124914382508933 21.005125838725938 32-33 21.18692183623726 26.730676040242727 31.11202725280448 20.97037487071553 34-35 21.667787734791442 26.645583353223252 31.014892124315608 20.671736787669698 36-37 21.62326559661162 26.388499126288867 31.19691192297455 20.79132335412496 38-39 21.263953802913065 26.84876587410079 31.113816163483527 20.773464159502616 40-41 21.449406809020928 27.098409020061375 30.75313520038594 20.699048970531756 42-43 21.17969205485318 27.073414377621248 30.481846050573836 21.26504751695174 44-45 21.510897698083006 26.914162613639363 30.33252001560818 21.24241967266945 46-47 21.9644006625151 27.390189569003553 29.670369807827225 20.975039960654122 48-49 21.899704182669012 27.545186278126156 29.354807212446026 21.20030232675881 50-51 21.614048791777872 27.913909200714592 29.39983075487665 21.072211252630893 52-53 21.98439907132163 28.18318253158881 28.66082987972024 21.171588517369322 54-55 21.592531516479703 27.910726813779736 28.823312430837326 21.673429238903232 56-57 21.93033465691699 28.06680842753922 28.621086206522445 21.381770709021346 58-59 22.300395628557585 27.702461286986207 28.614034326382708 21.383108758073497 60-61 21.84361461294219 27.86067654653156 29.31148335394652 20.98422548657973 62-63 21.62844185996051 27.782671903139715 29.463984782404296 21.124901454495486 64-65 21.645366372295875 28.039288013250303 29.03772575057319 21.27761986388063 66-67 21.66203774021597 27.914415489545135 28.70711914422722 21.716427626011672 68-69 21.635312922660766 27.936005091819094 28.936540311439956 21.49214167408018 70-71 21.84068537042261 27.98218586586239 28.73923232147894 21.43789644223606 72-73 21.917641272665467 27.5456925669567 28.956864191637553 21.57980196874028 74-75 21.57115889513312 28.537440059018813 28.350836461475037 21.540564584373033 76-77 21.720333282704452 27.902119903660466 28.60553590672713 21.772010906907948 78-79 21.816528160508025 27.877275587475857 28.44518700139591 21.861009250620203 80-81 21.791611517347626 27.99675975148451 28.394594281829296 21.81703444933857 82-83 21.791292523895113 27.96952130217958 28.65568980294444 21.583496370980864 84-85 21.58511879404283 28.06789078282052 28.413326168104653 21.933664255031992 86-87 21.40845936308865 28.139497038210344 28.327113213415206 22.124930385285797 88-89 21.59300164182235 28.03292323938059 28.12069202450438 22.25338309429268 90-91 21.620162263603675 27.7097309337734 28.23265908549237 22.437447717130553 92-93 21.492613420610095 27.771404806249212 28.423882971990672 22.31209880115003 94-95 21.40440905244429 27.73244081845206 28.479361497457706 22.383788631645945 96-97 21.128951765139846 27.72524428436074 28.719016931745035 22.426787018754386 98-99 21.170418090153504 27.927175335606574 28.1091140405979 22.793292533642028 100-101 20.835739946205194 27.96657155949599 28.371533528080604 22.82615496621821 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 512.0 1 382.5 2 310.5 3 376.5 4 339.5 5 339.0 6 607.0 7 1303.5 8 2080.0 9 2451.0 10 2372.0 11 2233.0 12 2321.0 13 2478.0 14 2646.0 15 2816.5 16 2947.0 17 3023.0 18 3190.5 19 3443.5 20 3643.0 21 3983.0 22 4719.5 23 5543.5 24 6359.5 25 7751.5 26 9746.0 27 11705.5 28 13671.0 29 15957.5 30 18504.0 31 21364.5 32 24301.5 33 26930.5 34 30003.5 35 32675.0 36 35577.0 37 38998.0 38 41124.5 39 42199.0 40 42796.0 41 44263.5 42 45862.5 43 47337.5 44 49811.5 45 51569.0 46 52075.5 47 55678.5 48 63350.5 49 61652.5 50 52502.5 51 46712.0 52 43075.0 53 41410.0 54 39772.0 55 35993.5 56 31097.5 57 27246.5 58 24390.5 59 21282.5 60 17404.5 61 14111.0 62 11340.5 63 8501.5 64 6104.0 65 4504.5 66 3245.0 67 2378.5 68 1739.0 69 1196.0 70 863.5 71 626.5 72 496.5 73 432.0 74 347.0 75 260.0 76 184.5 77 106.5 78 65.5 79 45.0 80 33.0 81 26.5 82 21.5 83 12.0 84 4.0 85 2.0 86 1.0 87 0.0 88 0.0 89 1.0 90 2.0 91 1.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07522005482384765 2 0.0 3 0.0 4 0.0 5 0.0016635204432197078 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.8081743948040302E-4 12-13 0.005243705744931687 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.594332458176927E-4 24-25 9.764141731941762E-4 26-27 5.786158063372896E-4 28-29 0.0033993678622315764 30-31 5.062888305451285E-4 32-33 0.0 34-35 0.0 36-37 1.446539515843224E-4 38-39 8.679237095059344E-4 40-41 2.169809273764836E-4 42-43 0.011283008223577147 44-45 1.084904636882418E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.6163487896080605E-4 84-85 5.062888305451285E-4 86-87 0.0 88-89 0.0 90-91 0.007702822921865167 92-93 0.0025314441527256423 94-95 0.0 96-97 0.0 98-99 1.084904636882418E-4 100-101 0.009727978244045681 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1382610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.95989698495422 #Duplication Level Percentage of deduplicated Percentage of total 1 78.07467846535287 45.25200320985276 2 12.725959260925713 14.751905755959568 3 4.007621630257937 6.968440105332729 4 1.7295479310463033 4.009776796559378 5 0.9396768748113965 2.7231787431606125 6 0.5500313226063714 1.9127855278058044 7 0.35052220442973053 1.4221361601780371 8 0.2565867770263143 1.1897394531317278 9 0.1924283614976112 1.0037815208446568 >10 1.0693158665836338 11.241885470088828 >50 0.06828170571190308 2.7052074759812648 >100 0.03209045521643923 3.242053698181213 >500 0.001504220554060731 0.5682247725102999 >1k 0.0012535171283839426 1.0603810963799805 >5k 5.01406851353577E-4 1.9485002140332097 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8998 0.6507981281778665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7504 0.5427416263443776 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5269 0.3810908354488974 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5048 0.3651065737988297 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2265 0.16382060016924513 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1870 0.13525144473134146 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1818 0.1314904419901491 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 7.23269757921612E-5 0.0 9 0.0 0.0 0.0 2.893079031686448E-4 0.0 10-11 0.0 0.0 0.0 3.61634878960806E-4 0.0 12-13 0.0 0.0 0.0 4.339618547529672E-4 0.0 14-15 0.0 0.0 0.0 4.339618547529672E-4 0.0 16-17 0.0 0.0 0.0 5.424523184412091E-4 0.0 18-19 0.0 0.0 0.0 5.786158063372896E-4 0.0 20-21 0.0 0.0 0.0 6.147792942333702E-4 0.0 22-23 0.0 0.0 0.0 6.509427821294508E-4 0.0 24-25 0.0 0.0 0.0 9.402506852980956E-4 0.0 26-27 0.0 0.0 0.0 0.001265722076362821 0.0 28-29 0.0 0.0 0.0 0.0026760981043099643 0.0 30-31 0.0 0.0 0.0 0.006473264333398428 0.0 32-33 0.0 0.0 0.0 0.012404076348355646 0.0 34-35 0.0 0.0 0.0 0.017828599532767737 0.0 36-37 0.0 0.0 0.0 0.02339777666876415 0.0 38-39 0.0 0.0 0.0 0.0300156949537469 0.0 40-41 0.0 0.0 0.0 0.03920122087935137 0.0 42-43 0.0 0.0 0.0 0.05048422910292852 0.0 44-45 0.0 0.0 0.0 0.06422635450343915 0.0 46-47 0.0 0.0 0.0 0.08256124286675201 0.0 48-49 0.0 0.0 0.0 0.10100462169375313 0.0 50-51 0.0 0.0 0.0 0.12895899783742343 0.0 52-53 0.0 0.0 0.0 0.1834573740968169 0.0 54-55 0.0 0.0 0.0 0.2548079357157839 0.0 56-57 0.0 0.0 0.0 0.32134875344457225 0.0 58-59 0.0 0.0 0.0 0.4242338765089215 0.0 60-61 0.0 0.0 0.0 0.554133125031643 0.0 62-63 0.0 0.0 0.0 0.6442525368686759 0.0 64-65 0.0 0.0 0.0 0.7188578123982902 0.0 66-67 1.446539515843224E-4 0.0 0.0 0.8967098458712146 0.0 68-69 1.446539515843224E-4 0.0 0.0 1.0804203643833041 0.0 70-71 1.446539515843224E-4 0.0 0.0 1.1883322122652085 0.0 72-73 1.446539515843224E-4 0.0 0.0 1.382421651803473 0.0 74-75 1.446539515843224E-4 0.0 0.0 1.6530691952177403 0.0 76-77 1.446539515843224E-4 0.0 0.0 1.9196302644997505 0.0 78-79 1.446539515843224E-4 0.0 0.0 2.2139648924859507 0.0 80-81 1.80817439480403E-4 0.0 0.0 2.554335640563861 0.0 82-83 2.169809273764836E-4 0.0 0.0 2.865486290421739 0.0 84-85 2.169809273764836E-4 0.0 0.0 3.18462907110465 0.0 86-87 2.169809273764836E-4 0.0 0.0 3.637974555369916 0.0 88-89 2.169809273764836E-4 0.0 0.0 4.228813620616082 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7545 0.0 26.140795 1 TACATGG 7550 0.0 25.983484 2 ACATGGG 7710 0.0 24.766575 3 GAGTACT 7400 0.0 23.527075 12-13 TACCTGG 1300 0.0 23.384651 2 CATGGGA 5075 0.0 22.65028 4 TAGACCG 105 0.0021964265 22.619081 5 GTACCTG 1495 0.0 22.252907 1 GTACTTT 7865 0.0 21.861925 14-15 GTATCAA 14790 0.0 21.433228 1 AGTACTT 7565 0.0 21.16151 12-13 TACACCG 180 5.1699353E-6 21.111143 5 ATACCGT 115 0.003724402 20.651459 6 GGTATCA 11640 0.0 19.924929 1 ATGGGAG 1960 0.0 19.87248 5 CGTACAC 145 6.1468413E-4 19.655201 3 AGAGTAC 12130 0.0 19.463514 10-11 CATGGGG 2665 0.0 19.24956 4 ACTTTTT 9175 0.0 18.714611 16-17 ATGGGAT 2040 0.0 18.62748 5 >>END_MODULE