FastQCFastQC Report
Thu 26 May 2016
SRR937427_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937427_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943974
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136940.7044332897456447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100220.5155418745312437No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT93700.4820023313069002No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75370.38771094675134543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66610.3426486156707857No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56750.2919277726965484No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40550.2085933248078421No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34220.1760311609105883No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31570.16239929134854683No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29830.1534485543530932No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28110.14460069939207007No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27070.13925083360168397No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25230.1297856864340778No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23880.12284114911001896No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20020.10298491646493216No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19800.10185321408619663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC804.4913922E-429.691319
GGCGCGA908.943073E-426.3922758
GAGTACT111900.024.91668312-13
GTACATG117650.024.807521
TACATGG116350.024.7061732
ACATGGG115800.023.715693
GTGTAGC13650.023.680081
GTACTTT121200.022.9447914-15
ATGGGCG2903.6379788E-1222.9398925
GTATCAA239250.022.8085061
AGTACTT115300.022.63708912-13
AGAGTAC179800.021.69104410-11
CATGGGG36700.020.9748614
GGTATCA185100.020.4672181
CATGGGT16150.020.007214
GTCGGGA5000.019.9556472
ACTTTTT141300.019.89911516-17
TCAACGC275600.019.8102934
ATCAACG275850.019.790813
CAACGCA276000.019.7820955
Produced by FastQC (version 0.11.5)