##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937427_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943974 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.85723728815303 33.0 31.0 34.0 30.0 34.0 2 31.961245366450374 33.0 31.0 34.0 30.0 34.0 3 31.98577861638067 33.0 31.0 34.0 30.0 34.0 4 35.616025214329 37.0 35.0 37.0 33.0 37.0 5 35.64009961038573 37.0 35.0 37.0 33.0 37.0 6 35.694538610084294 37.0 35.0 37.0 33.0 37.0 7 35.64602973085031 37.0 35.0 37.0 33.0 37.0 8 35.631873677322844 37.0 35.0 37.0 33.0 37.0 9 37.24367609854864 39.0 37.0 39.0 34.0 39.0 10-11 37.14216085194555 39.0 37.0 39.0 34.0 39.0 12-13 37.01397138027566 39.0 37.0 39.0 33.0 39.0 14-15 38.089215956592014 40.0 38.0 41.0 33.0 41.0 16-17 38.030772788113424 40.0 38.0 41.0 33.0 41.0 18-19 38.018794490049764 40.0 38.0 41.0 33.0 41.0 20-21 37.920055515145776 40.0 38.0 41.0 33.0 41.0 22-23 37.98430663167306 40.0 38.0 41.0 33.0 41.0 24-25 37.94221347610616 40.0 38.0 41.0 33.0 41.0 26-27 37.853804629074254 40.0 38.0 41.0 33.0 41.0 28-29 37.72163465149225 40.0 37.0 41.0 33.0 41.0 30-31 37.44438274380213 39.5 37.0 41.0 32.5 41.0 32-33 37.14854519659214 39.0 36.0 41.0 32.0 41.0 34-35 37.09170210095402 39.0 36.0 41.0 31.5 41.0 36-37 36.933817787686465 39.0 36.0 41.0 31.0 41.0 38-39 36.764644485985926 39.0 36.0 41.0 31.0 41.0 40-41 36.52639927282978 39.0 35.0 41.0 30.5 41.0 42-43 36.19787070197441 39.0 35.0 41.0 30.0 41.0 44-45 35.84675463766491 39.0 35.0 40.5 29.5 41.0 46-47 35.32832820809332 39.0 35.0 40.0 26.5 41.0 48-49 34.890835474137006 38.5 35.0 40.0 24.0 41.0 50-51 33.89494123892604 37.5 33.5 39.5 23.5 40.5 52-53 33.986987994695404 38.0 33.5 39.5 23.0 40.5 54-55 34.296904948317206 38.0 34.0 40.0 22.5 41.0 56-57 34.02388149224218 38.0 34.0 40.0 21.0 41.0 58-59 33.764460069939204 38.0 33.5 40.0 19.5 41.0 60-61 33.412429641548705 38.0 33.0 40.0 18.0 41.0 62-63 33.04780490891339 37.0 33.0 40.0 15.0 41.0 64-65 32.6408280151895 36.5 32.0 39.5 11.0 41.0 66-67 32.1833386146111 36.0 31.5 39.0 8.0 40.5 68-69 31.68579492318313 35.5 31.0 39.0 6.5 40.0 70-71 31.19268827669506 35.0 30.5 38.5 2.0 40.0 72-73 30.697621470246002 35.0 30.0 37.5 2.0 40.0 74-75 30.149080954786434 34.5 29.0 37.0 2.0 39.0 76-77 29.622423705255315 34.0 29.0 36.5 2.0 39.0 78-79 29.057970939940553 34.0 28.5 36.0 2.0 38.5 80-81 28.444973029474674 34.0 27.5 35.5 2.0 37.0 82-83 27.86656534500976 33.5 26.0 35.0 2.0 37.0 84-85 27.257766050368986 33.0 26.0 35.0 2.0 36.0 86-87 26.668751228154285 32.5 25.0 35.0 2.0 36.0 88-89 26.07683744741442 32.0 23.5 35.0 2.0 35.5 90-91 25.474026658792763 32.0 20.5 34.5 2.0 35.0 92-93 24.78629420969622 31.5 18.5 34.0 2.0 35.0 94-95 23.99138131477067 31.0 6.0 34.0 2.0 35.0 96-97 23.11942006426012 31.0 2.0 34.0 2.0 35.0 98-99 21.93389597803263 30.5 2.0 34.0 2.0 35.0 100-101 18.84667310365262 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06980266929749845 1101 2 0.08094077704023661 1101 3 0.1832484320373986 1101 4 0.03379516071125721 1101 5 -0.01782357041628302 1101 6 0.09658883520022243 1101 7 0.1841048090876285 1101 8 0.0916376611468337 1101 9 0.21845196703523584 1101 10-11 0.2592031263938708 1101 12-13 0.2727233096711643 1101 14-15 0.38829192827082437 1101 16-17 0.37917523368699335 1101 18-19 0.33380040991806226 1101 20-21 0.4526908756539143 1101 22-23 0.4796063892508542 1101 24-25 0.49869559974243316 1101 26-27 0.5305164029149836 1101 28-29 0.49749589106408365 1101 30-31 0.45414795376171213 1101 32-33 0.4085559704353088 1101 34-35 0.5436812352948053 1101 36-37 0.6493519182063423 1101 38-39 0.6066910657491036 1101 40-41 0.7066621777883384 1101 42-43 0.7891320185754296 1101 44-45 0.9615665111834986 1101 46-47 0.8955813141012285 1101 48-49 0.9742749638479964 1101 50-51 0.9375908114371541 1101 52-53 0.7777844176778999 1101 54-55 0.8829225660566706 1101 56-57 1.0640583107287682 1101 58-59 1.0714867220436943 1101 60-61 1.1724308075121357 1101 62-63 1.1786909917954986 1101 64-65 1.1167476567807597 1101 66-67 1.3047642338759857 1101 68-69 1.2979780176275533 1101 70-71 1.2992136653179607 1101 72-73 1.3252354891486178 1101 74-75 1.5119235597822112 1101 76-77 1.595929590059395 1101 78-79 1.6701178582440939 1101 80-81 1.6871001705577697 1101 82-83 1.573676241399685 1101 84-85 1.706434368914877 1101 86-87 1.667707775157652 1101 88-89 1.7226096558571875 1101 90-91 1.7237423777646974 1101 92-93 1.8942211874364965 1101 94-95 1.958880263276388 1101 96-97 1.9335363908959167 1101 98-99 2.325058939990761 1101 100-101 2.5133724384833123 1102 1 0.1802467697645298 1102 2 0.2317965563782316 1102 3 0.10575372105973813 1102 4 0.12073914807969999 1102 5 0.06667216510155782 1102 6 -7.89051585876166E-4 1102 7 -0.04232078756287905 1102 8 -0.00932389863796601 1102 9 0.1151375130353145 1102 10-11 0.13200491113398272 1102 12-13 0.24019859430262613 1102 14-15 0.36698725840782487 1102 16-17 0.36111937628879787 1102 18-19 0.49427954877208435 1102 20-21 0.315299372684521 1102 22-23 0.3999184526570332 1102 24-25 0.37142531185107686 1102 26-27 0.39026084445187337 1102 28-29 0.37669550785944494 1102 30-31 0.376164257699763 1102 32-33 0.3446397432268924 1102 34-35 0.5901447975899501 1102 36-37 0.802224622415828 1102 38-39 0.6873137289326294 1102 40-41 0.7190642144500217 1102 42-43 0.8832854867104558 1102 44-45 0.8502231660376438 1102 46-47 0.9224023122960929 1102 48-49 0.979603903338031 1102 50-51 0.7897399064266608 1102 52-53 0.7783499106325209 1102 54-55 0.8935161340732805 1102 56-57 0.9298801383132869 1102 58-59 1.0480209820462676 1102 60-61 1.0093326601114114 1102 62-63 1.082350950902601 1102 64-65 1.0159391127366177 1102 66-67 1.1246680335451202 1102 68-69 1.3316891593910931 1102 70-71 1.2625031932754887 1102 72-73 1.3728045347295712 1102 74-75 1.3641857533626442 1102 76-77 1.3971843191328297 1102 78-79 1.5179159839121503 1102 80-81 1.684537711306529 1102 82-83 1.7467980286235338 1102 84-85 1.7593534213704416 1102 86-87 1.7679741112511813 1102 88-89 1.722730516116119 1102 90-91 1.8558747962560922 1102 92-93 1.870886286985435 1102 94-95 1.9684093739666437 1102 96-97 1.986686075772198 1102 98-99 2.0756931791477093 1102 100-101 2.067021087731316 1103 1 0.293265189786716 1103 2 0.2852918466825649 1103 3 0.21035636994630735 1103 4 0.10665447507542325 1103 5 0.22794593111778028 1103 6 0.18551992837888776 1103 7 0.2512919369543667 1103 8 0.24258454328303714 1103 9 0.22831568745720432 1103 10-11 0.27085199195656884 1103 12-13 0.29574916461449874 1103 14-15 0.4126942384741028 1103 16-17 0.4146185188268845 1103 18-19 0.4352305837086732 1103 20-21 0.2576151180781494 1103 22-23 0.21489793158124115 1103 24-25 0.2729227884113712 1103 26-27 0.37555573521159147 1103 28-29 0.17168993798264864 1103 30-31 0.3400569308418113 1103 32-33 0.33515837625494527 1103 34-35 0.41150606822886004 1103 36-37 0.4001931582769558 1103 38-39 0.5617953768910411 1103 40-41 0.6242920128938962 1103 42-43 0.43700092084360875 1103 44-45 0.6205179797526839 1103 46-47 0.533058066717544 1103 48-49 0.8828683088794449 1103 50-51 0.6611632206891684 1103 52-53 0.7028157644325503 1103 54-55 0.8636889679395807 1103 56-57 0.9038045946498059 1103 58-59 0.9995645267901949 1103 60-61 0.9784504351337091 1103 62-63 1.0411479118980438 1103 64-65 0.8372532501625614 1103 66-67 1.034445320049798 1103 68-69 1.2560596137552658 1103 70-71 1.3860172507562503 1103 72-73 1.242621467517612 1103 74-75 1.1958674981254198 1103 76-77 1.2710007769631062 1103 78-79 1.2563341298365778 1103 80-81 1.3485114399787506 1103 82-83 1.5740814288497766 1103 84-85 1.6673892716173526 1103 86-87 1.6776461411336392 1103 88-89 1.72941006283963 1103 90-91 1.529681011676633 1103 92-93 1.5127081986722928 1103 94-95 1.5807707615321753 1103 96-97 1.6206187260365041 1103 98-99 1.7908623012734886 1103 100-101 1.7089295613765962 1104 1 0.2252294755010027 1104 2 0.27505375144447086 1104 3 0.20388811597805656 1104 4 0.1253052687262226 1104 5 0.1594340263558749 1104 6 0.2065913569503124 1104 7 0.10573638139881325 1104 8 0.20564009883859313 1104 9 0.23938711602863805 1104 10-11 0.2990761983057766 1104 12-13 0.30713805350339385 1104 14-15 0.5662259845058486 1104 16-17 0.4315034394618067 1104 18-19 0.4523436789467681 1104 20-21 0.4455913085543415 1104 22-23 0.46521539189870253 1104 24-25 0.3699465979351757 1104 26-27 0.31511922727508335 1104 28-29 0.39908081099851955 1104 30-31 0.3942732006830809 1104 32-33 0.4901484556200302 1104 34-35 0.6614465444193414 1104 36-37 0.6690919678007603 1104 38-39 0.6658727578434167 1104 40-41 0.5884189970208809 1104 42-43 0.5784790954467738 1104 44-45 0.6735437734034733 1104 46-47 0.8510640190984944 1104 48-49 0.7072829914191274 1104 50-51 0.6263219508479025 1104 52-53 0.6871411612579479 1104 54-55 0.8891651584157714 1104 56-57 0.9630109438561547 1104 58-59 0.985705399806065 1104 60-61 0.9205932922765712 1104 62-63 0.8672193404694823 1104 64-65 0.9702095993689142 1104 66-67 0.9756159549704293 1104 68-69 0.7802461216917713 1104 70-71 0.896800980914982 1104 72-73 1.051768292914435 1104 74-75 1.048218688601608 1104 76-77 1.199869824582155 1104 78-79 1.1861555584080072 1104 80-81 1.1662892177565283 1104 82-83 1.4310754764688234 1104 84-85 1.3228654620935387 1104 86-87 1.3510886014510994 1104 88-89 1.4579616501412218 1104 90-91 1.6758913291369488 1104 92-93 1.9992657383548327 1104 94-95 2.024431475817888 1104 96-97 1.8839520593698396 1104 98-99 2.330773015559206 1104 100-101 2.131875989948025 1105 1 0.130669779480737 1105 2 0.0935185204598703 1105 3 0.20497293254583937 1105 4 0.1897387509385169 1105 5 0.17350442166285518 1105 6 0.2097891437645245 1105 7 0.2044524669931036 1105 8 0.25834414079135115 1105 9 0.26747835439051926 1105 10-11 0.2672405429050215 1105 12-13 0.36018607252434265 1105 14-15 0.4644431033645944 1105 16-17 0.42139977970579423 1105 18-19 0.4904022528196279 1105 20-21 0.4218611127896139 1105 22-23 0.5082181917120394 1105 24-25 0.43447174070343664 1105 26-27 0.5251962221420996 1105 28-29 0.4398534261575051 1105 30-31 0.441455610744633 1105 32-33 0.5472573150294053 1105 34-35 0.6249314232051901 1105 36-37 0.7955574256591333 1105 38-39 0.66810460905333 1105 40-41 0.6081579033160196 1105 42-43 0.6304670740259866 1105 44-45 0.7409820552957953 1105 46-47 0.7807009321931773 1105 48-49 0.8679353923701782 1105 50-51 0.7068036965093043 1105 52-53 0.640664815236569 1105 54-55 1.0464740044927012 1105 56-57 1.071878574902975 1105 58-59 0.9749260073211161 1105 60-61 1.02999977628874 1105 62-63 0.9872112298990672 1105 64-65 1.1477795391507044 1105 66-67 1.1165048957077133 1105 68-69 1.0001713488988493 1105 70-71 1.0610223661559637 1105 72-73 1.3003784966786291 1105 74-75 1.1918875551216175 1105 76-77 1.1857141016303494 1105 78-79 1.182399475480203 1105 80-81 1.1859491293179794 1105 82-83 1.3151087631385998 1105 84-85 1.370964588818424 1105 86-87 1.3241452865609808 1105 88-89 1.2554622612115942 1105 90-91 1.534465646404687 1105 92-93 1.8020494416635024 1105 94-95 1.9265077818419343 1105 96-97 1.7757583833926809 1105 98-99 1.9213768641915188 1105 100-101 1.9606228512683828 1106 1 0.0369636694507669 1106 2 -0.5391923019033804 1106 3 -0.06893299504664796 1106 4 0.1335303769513274 1106 5 0.009743537204307984 1106 6 -0.013159012440773665 1106 7 0.19793328629032914 1106 8 0.03112056647474759 1106 9 0.18010853034286356 1106 10-11 -0.3239712133643877 1106 12-13 -0.20734259558803814 1106 14-15 -0.17145618362541626 1106 16-17 0.08557179625471178 1106 18-19 0.04697908346241064 1106 20-21 -0.030842058477823286 1106 22-23 0.00857140714094129 1106 24-25 0.03227583556381575 1106 26-27 0.04266685928379843 1106 28-29 0.3167003770612027 1106 30-31 0.2654049631322053 1106 32-33 0.11871288520242018 1106 34-35 0.3037823922042904 1106 36-37 0.22765471244135682 1106 38-39 0.3505154205699128 1106 40-41 0.47867199751340195 1106 42-43 0.273382405709377 1106 44-45 0.32489237490177914 1106 46-47 0.4551299132841464 1106 48-49 0.4014805702873687 1106 50-51 0.26007470507969543 1106 52-53 0.34491846379572877 1106 54-55 0.6020985124868545 1106 56-57 0.30381841981333224 1106 58-59 0.038102035106888366 1106 60-61 0.277621573376706 1106 62-63 0.5112482955463378 1106 64-65 0.4319645310639686 1106 66-67 0.5872517742409826 1106 68-69 0.5531653481738736 1106 70-71 0.5065774020985891 1106 72-73 0.5674795975778366 1106 74-75 0.5416284923706947 1106 76-77 0.7870733396299805 1106 78-79 0.8530538397673659 1106 80-81 0.7740229710292041 1106 82-83 0.8326450645653587 1106 84-85 0.6761639300926063 1106 86-87 0.7664586405935943 1106 88-89 0.9056579458614671 1106 90-91 0.9555470825651575 1106 92-93 1.1812089946992863 1106 94-95 1.0792247464195448 1106 96-97 1.0892593668328345 1106 98-99 1.0576894424456924 1106 100-101 0.6313861903264915 1107 1 0.10224007033515647 1107 2 0.22792335881237946 1107 3 0.16207706872832617 1107 4 0.18376613247065166 1107 5 -9.516810051053426E-4 1107 6 0.06787312414095226 1107 7 0.17702230342636938 1107 8 0.11216642434698798 1107 9 0.17251247669156555 1107 10-11 0.20973104676964027 1107 12-13 0.28388527784149886 1107 14-15 0.3434128136728063 1107 16-17 0.3503688227455086 1107 18-19 0.3487555156155153 1107 20-21 0.3025234231355114 1107 22-23 0.44890661826806166 1107 24-25 0.3838035561329036 1107 26-27 0.25428306634439934 1107 28-29 0.41125656060035 1107 30-31 0.3390911957434426 1107 32-33 0.33869010786018094 1107 34-35 0.5042000471776831 1107 36-37 0.5807630201286287 1107 38-39 0.5383663524698221 1107 40-41 0.7820507861398269 1107 42-43 0.667374485886036 1107 44-45 0.7762588439202958 1107 46-47 0.8819702351961851 1107 48-49 1.016216814099927 1107 50-51 0.9736713957155203 1107 52-53 1.1002802559651528 1107 54-55 1.0156723255094633 1107 56-57 1.1779584313339413 1107 58-59 1.1114052590032557 1107 60-61 1.0719313805899375 1107 62-63 1.2470705742252193 1107 64-65 1.068145568715451 1107 66-67 1.4241981168705777 1107 68-69 1.5164161699340504 1107 70-71 1.412754515988734 1107 72-73 1.4834882799882791 1107 74-75 1.4257717204784441 1107 76-77 1.3833508854505148 1107 78-79 1.4615765818828343 1107 80-81 1.5614593352460844 1107 82-83 1.7421838946300774 1107 84-85 1.63069975334761 1107 86-87 1.5570131680785266 1107 88-89 1.5834774269803127 1107 90-91 1.7996643827112635 1107 92-93 1.9927836156948153 1107 94-95 2.2430391438002566 1107 96-97 2.0742403588874154 1107 98-99 2.2068952831768662 1107 100-101 2.0053818644257895 1108 1 0.1764032985273758 1108 2 0.23140535824976283 1108 3 0.2414666559006413 1108 4 0.16933263392560605 1108 5 0.07865537841276904 1108 6 0.0314837865119344 1108 7 0.17392973813659296 1108 8 0.014220529120350989 1108 9 0.16076347753732279 1108 10-11 0.2369553953532133 1108 12-13 0.13664205926448147 1108 14-15 0.23544868681928932 1108 16-17 0.1290833296409346 1108 18-19 0.32409181109187557 1108 20-21 0.3282279095085272 1108 22-23 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-0.6635849196493737 2316 52-53 -0.5926646725146725 2316 54-55 -0.31377105666066285 2316 56-57 -0.37269631321641583 2316 58-59 -0.48327808288591 2316 60-61 -0.6190236467499659 2316 62-63 -0.6906413167085219 2316 64-65 -0.6004352785084386 2316 66-67 -0.5261368125572439 2316 68-69 -0.23965987022529234 2316 70-71 -0.11503613031327475 2316 72-73 -0.31667574152590916 2316 74-75 -0.1809132741465902 2316 76-77 -0.11850128770039703 2316 78-79 -0.3594770193463397 2316 80-81 -0.1260258411083477 2316 82-83 -0.026919603481974974 2316 84-85 0.15844532503502506 2316 86-87 0.2872843491228636 2316 88-89 0.07195567579576334 2316 90-91 0.25976762504276607 2316 92-93 0.4538563199749355 2316 94-95 0.5962431082199302 2316 96-97 0.6808453997318153 2316 98-99 1.2570715571160527 2316 100-101 1.928540020874049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6682.0 3 1094.0 4 1009.0 5 1103.0 6 1336.0 7 1721.0 8 2095.0 9 2780.0 10 3645.0 11 5159.0 12 7802.0 13 11024.0 14 12365.0 15 16392.0 16 21859.0 17 30117.0 18 24975.0 19 15220.0 20 14730.0 21 15225.0 22 16680.0 23 18432.0 24 20973.0 25 23932.0 26 27866.0 27 32856.0 28 39227.0 29 46261.0 30 56319.0 31 68822.0 32 86909.0 33 111281.0 34 146962.0 35 197662.0 36 266526.0 37 333609.0 38 230582.0 39 22738.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.67713809502438 19.65718052961213 15.535722484192782 21.129958891170705 2 14.636558702845864 21.184055891117932 41.138738210982986 23.040647195053214 3 18.830376908689715 26.488186247790996 31.17991071681803 23.50152612670126 4 11.497355157623119 17.393358037092767 40.10785635241738 31.001430452866735 5 12.040284341859978 38.79683273902069 34.910775752043996 14.252107167075337 6 28.694002978637634 38.01046801934001 18.535652663426834 14.759876338595523 7 25.901822564664084 34.60965442722269 22.0183689608278 17.470154047285433 8 25.54477883551623 33.29210868460205 21.020601326543552 20.142511153338166 9 26.269680786891197 16.639409934717893 22.021084955418228 35.06982432297268 10-11 23.780218205030433 27.104365776372198 28.224265622433986 20.891150396163376 12-13 25.783832541098956 25.16982619480278 28.499040888986737 20.547300375111526 14-15 22.5249840828773 26.012367668779497 28.673091942971592 22.78955630537161 16-17 20.226096199785758 29.34216671701451 28.84596548783971 21.585771595360026 18-19 19.949429151457657 27.744174700607022 32.222795231361246 20.083600916574078 20-21 21.721802552076742 26.079415984797542 31.954362729545277 20.244418733580435 22-23 20.778591842893995 26.351766336879656 32.1358440342074 20.733797786018947 24-25 20.87647100213071 26.47444292064559 32.16978790558473 20.479298171638973 26-27 20.561966201308167 26.395540723142837 32.66374422661736 20.378748848931636 28-29 20.064522132313158 27.278412021448606 32.268644259368045 20.38842158687019 30-31 20.457877398387314 26.563287469803047 32.10287289607403 20.875962235735606 32-33 20.322548465915137 26.674612750835873 32.117096135847106 20.88574264740188 34-35 21.063008616794306 26.73554465671886 31.7865342515576 20.414912474929224 36-37 20.753863029968123 26.296182126346956 32.24336160929684 20.70659323438808 38-39 20.36932565711056 27.03675345155555 31.945718359871005 20.648202531462882 40-41 20.636319638049912 27.06920110073566 31.715397259087798 20.57908200212663 42-43 20.331931919754467 27.067995707097005 31.110453589945287 21.489618783203245 44-45 20.798868891050354 26.84595135262466 30.974777363182156 21.380402393142834 46-47 21.291000888734924 27.31390694883485 30.465126948876094 20.929965213554137 48-49 21.018803727236406 27.419030557667774 30.146382956786226 21.415782758309597 50-51 20.61733684547554 27.744918040562872 30.344867410444753 21.292877703516833 52-53 21.09570760711259 28.188884984372336 29.269505058268248 21.445902350246822 54-55 20.896349546886434 27.548613980545383 29.685028267883595 21.87000820468458 56-57 21.40478389684613 27.67273407652833 29.101558070442017 21.82092395618352 58-59 21.74717170855578 27.52000181418845 29.309889414390796 21.422937062864982 60-61 21.37238231920443 27.72547921072902 29.997879079711296 20.90425939035525 62-63 21.001710726860473 27.787559577776737 29.91511825869757 21.295611436665222 64-65 20.817084273831004 28.182099074831186 29.57403991927805 21.426776732059764 66-67 21.106909032807568 27.81856299230354 29.022189980040487 22.052337994848408 68-69 21.073965912160002 27.694669110446334 29.40733360684973 21.824031370543935 70-71 21.44189901459805 27.61779929303415 29.26102069869833 21.67928099366947 72-73 21.165677928208286 27.26853816958263 29.889925691917192 21.675858210291903 74-75 20.689013534507374 28.33777817180546 29.2584885762325 21.71471971745466 76-77 20.69515480359722 27.60667586969095 29.40747713075958 22.29069219595225 78-79 20.87617779030983 27.67740537018513 29.38771270589503 22.058704133610014 80-81 21.087622916977015 27.830460266315605 29.040004041176704 22.041912775530676 82-83 20.974692026184215 27.53608061440132 29.52543683315293 21.963790526261533 84-85 20.935591892584867 27.50197454289171 29.27894718532615 22.283486379197274 86-87 20.696485848588623 27.759783557402823 29.21967331062703 22.324057283381528 88-89 20.82585324544327 27.314987338614365 28.97511585438453 22.88404356155784 90-91 20.794231455627127 26.892049825338145 29.360097207600848 22.953621511433877 92-93 21.173587137627234 27.028095527111674 29.603173069117084 22.195144266144005 94-95 20.718285010073895 26.840399492207613 29.71876589750449 22.722549600214002 96-97 20.106415331642648 27.03866930578751 30.080049895878435 22.77486546669141 98-99 20.4381121599913 27.25216500674869 29.116231886971455 23.193490946288552 100-101 20.707814403786802 26.08557724619784 30.180877141260854 23.025731208754507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2945.0 1 1695.0 2 490.0 3 533.0 4 479.0 5 462.0 6 739.5 7 1530.0 8 2623.0 9 3230.0 10 3100.5 11 2936.0 12 3061.0 13 3265.0 14 3497.5 15 3685.0 16 3897.5 17 4177.0 18 4495.5 19 4811.0 20 5282.5 21 5921.0 22 6869.0 23 8145.0 24 9692.0 25 11745.5 26 14823.0 27 18290.5 28 20761.5 29 23752.0 30 27978.5 31 32117.5 32 36509.0 33 42028.0 34 48664.0 35 53568.5 36 59178.5 37 64654.0 38 65576.5 39 65408.5 40 65563.0 41 65944.0 42 66568.5 43 67937.5 44 70186.5 45 71206.5 46 71107.0 47 77600.5 48 91426.5 49 88441.5 50 70791.0 51 59510.5 52 52884.0 53 48790.0 54 45210.5 55 40768.0 56 36231.0 57 31885.0 58 28925.5 59 26089.5 60 22380.0 61 18929.5 62 15179.0 63 10845.0 64 7431.5 65 5503.0 66 3981.0 67 2836.5 68 2071.0 69 1536.0 70 1153.5 71 874.0 72 688.0 73 604.5 74 495.5 75 373.0 76 281.0 77 191.0 78 139.0 79 92.5 80 48.5 81 35.5 82 26.5 83 19.0 84 11.5 85 6.0 86 4.0 87 3.5 88 4.5 89 3.0 90 2.0 91 1.5 92 1.5 93 2.5 94 3.5 95 2.0 96 0.0 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28091939501248475 2 0.24563085720282268 3 0.24439627278965664 4 0.24321312939370587 5 0.24480780092737864 6 0.21404607263265868 7 0.21502345195974845 8 0.21584650823519244 9 0.22592894760938162 10-11 0.23454531799293613 12-13 0.2272406935483705 14-15 0.21805847197544823 16-17 0.2127600472022774 18-19 0.20182883104403662 20-21 0.2070243737827769 22-23 0.20496673309416688 24-25 0.20640708157619392 26-27 0.20208603613011286 28-29 0.21474052636506455 30-31 0.22713781151394 32-33 0.21782698739797962 34-35 0.21684960807088985 36-37 0.20813035565290483 38-39 0.1991024571316283 40-41 0.19635036271061238 42-43 0.2072044173430303 44-45 0.20221463867315098 46-47 0.21286292923670788 48-49 0.2160008312868382 50-51 0.2272149730397629 52-53 0.23788898411192744 54-55 0.21751834129468808 56-57 0.1996940288296037 58-59 0.1909747764116187 60-61 0.19436988354782522 62-63 0.19871664950251391 64-65 0.19205503777313893 66-67 0.19529582185769975 68-69 0.19599027559010565 70-71 0.19815079831314616 72-73 0.19935966221770454 74-75 0.2004142030706172 76-77 0.2074101814118913 78-79 0.20882480938531073 80-81 0.20293481291416446 82-83 0.1992825006918817 84-85 0.18783687436148838 86-87 0.18984307403288317 88-89 0.19665900881390388 90-91 0.19529582185769975 92-93 0.20043992357922483 94-95 0.1960417166073209 96-97 0.2023689617247967 98-99 0.2041436768187229 100-101 0.1935211067637736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943974.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.36775801138467 #Duplication Level Percentage of deduplicated Percentage of total 1 71.20791107143029 34.4416701119914 2 14.689434363547644 14.209900132403815 3 5.833966676720431 8.465276653982876 4 2.809001959846979 5.4346050818953575 5 1.5681128421765538 3.79230512424701 6 0.9600568501427768 2.7861478442926817 7 0.6163718501652218 2.086876714567463 8 0.4399060825517993 1.702181675888131 9 0.29324694598125134 1.276532758871888 >10 1.418368692859568 12.21011331514036 >50 0.10610886389356283 3.5430010934129585 >100 0.052721996010409646 4.471889233536304 >500 0.0020232064177266207 0.68124590316691 >1k 0.002129690966028022 2.170644030314465 >5k 4.2593819320560446E-4 1.5061369884561844 >10k+ 2.1296909660280223E-4 1.2214733378321947 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13694 0.7044332897456447 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10022 0.5155418745312437 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9370 0.4820023313069002 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7537 0.38771094675134543 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6661 0.3426486156707857 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5675 0.2919277726965484 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4055 0.2085933248078421 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3422 0.1760311609105883 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3157 0.16239929134854683 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2983 0.1534485543530932 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2811 0.14460069939207007 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2707 0.13925083360168397 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2523 0.1297856864340778 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2388 0.12284114911001896 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2002 0.10298491646493216 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1980 0.10185321408619663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.144101721525082E-5 0.0 4 0.0 0.0 0.0 5.144101721525082E-5 0.0 5 0.0 0.0 0.0 5.144101721525082E-5 0.0 6 0.0 0.0 0.0 5.144101721525082E-5 0.0 7 0.0 0.0 0.0 1.0288203443050164E-4 0.0 8 0.0 0.0 0.0 1.0288203443050164E-4 0.0 9 0.0 0.0 0.0 1.0288203443050164E-4 0.0 10-11 0.0 0.0 0.0 1.0288203443050164E-4 0.0 12-13 0.0 0.0 0.0 1.0288203443050164E-4 0.0 14-15 0.0 0.0 0.0 1.0288203443050164E-4 0.0 16-17 0.0 0.0 0.0 1.2860254303812707E-4 0.0 18-19 0.0 0.0 0.0 1.5432305164575247E-4 0.0 20-21 0.0 0.0 0.0 2.5720508607625413E-4 0.0 22-23 0.0 0.0 0.0 5.915716979753845E-4 0.0 24-25 0.0 0.0 0.0 7.973357668363877E-4 0.0 26-27 0.0 0.0 0.0 0.0011317023787355183 0.0 28-29 0.0 0.0 0.0 0.0028035354382311695 0.0 30-31 0.0 0.0 0.0 0.0055041888420318385 0.0 32-33 0.0 0.0 0.0 0.008204842245832506 0.0 34-35 0.0 0.0 0.0 0.012757372269382204 0.0 36-37 0.0 0.0 0.0 0.018698809757743674 0.0 38-39 0.0 0.0 0.0 0.028498323537248953 0.0 40-41 0.0 0.0 0.0 0.039815347324604136 0.0 42-43 0.0 0.0 0.0 0.051363855689427945 0.0 44-45 0.0 0.0 0.0 0.06023743115905871 0.0 46-47 0.0 0.0 0.0 0.07240323173046553 0.0 48-49 0.0 0.0 0.0 0.08408034263832748 0.0 50-51 0.0 0.0 0.0 0.09673483287327916 0.0 52-53 0.0 0.0 0.0 0.12407573352318499 0.0 54-55 0.0 0.0 0.0 0.17109282325792424 0.0 56-57 0.0 0.0 0.0 0.24439627278965664 0.0 58-59 0.0 0.0 0.0 0.3354983142778658 0.0 60-61 0.0 0.0 0.0 0.45000601859901423 0.0 62-63 0.0 0.0 0.0 0.5208917403216298 0.0 64-65 0.0 0.0 0.0 0.6152860069116151 0.0 66-67 0.0 0.0 0.0 0.7831123255763709 0.0 68-69 0.0 0.0 0.0 0.9948949934515585 0.0 70-71 0.0 0.0 0.0 1.2235503149733484 0.0 72-73 0.0 0.0 0.0 1.5011003233582343 0.0 74-75 0.0 0.0 0.0 1.778573170217297 2.572050860762541E-5 76-77 0.0 0.0 0.0 2.029811098296582 5.144101721525082E-5 78-79 0.0 0.0 0.0 2.289022384044231 5.144101721525082E-5 80-81 0.0 0.0 0.0 2.559859339682527 5.144101721525082E-5 82-83 0.0 0.0 0.0 2.9136963765976294 5.144101721525082E-5 84-85 0.0 0.0 0.0 3.421419216512155 5.144101721525082E-5 86-87 0.0 5.144101721525082E-5 0.0 4.0203212594407125 5.144101721525082E-5 88-89 0.0 5.144101721525082E-5 0.0 4.685582214576944 5.144101721525082E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGAC 80 4.4913922E-4 29.69131 9 GGCGCGA 90 8.943073E-4 26.392275 8 GAGTACT 11190 0.0 24.916683 12-13 GTACATG 11765 0.0 24.80752 1 TACATGG 11635 0.0 24.706173 2 ACATGGG 11580 0.0 23.71569 3 GTGTAGC 1365 0.0 23.68008 1 GTACTTT 12120 0.0 22.94479 14-15 ATGGGCG 290 3.6379788E-12 22.939892 5 GTATCAA 23925 0.0 22.808506 1 AGTACTT 11530 0.0 22.637089 12-13 AGAGTAC 17980 0.0 21.691044 10-11 CATGGGG 3670 0.0 20.974861 4 GGTATCA 18510 0.0 20.467218 1 CATGGGT 1615 0.0 20.00721 4 GTCGGGA 500 0.0 19.955647 2 ACTTTTT 14130 0.0 19.899115 16-17 TCAACGC 27560 0.0 19.810293 4 ATCAACG 27585 0.0 19.79081 3 CAACGCA 27600 0.0 19.782095 5 >>END_MODULE