FastQCFastQC Report
Thu 26 May 2016
SRR937427_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937427_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943974
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141620.7285076858023821No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT132110.6795872784306786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114090.5868905654087966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.40211443157161564No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53490.27515800108437666No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45860.23590850494914026No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36360.187039538594652No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36190.1861650413019927No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35220.1811752626321134No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28590.1470698682184021No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28260.14537231465029882No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28170.14490934549536155No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28070.14439493532320907No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26280.13518699324167915No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20820.1071001978421522No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19600.10082439374189162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA950.001226790525.0002449
GTACATG121350.023.4191721
GAGTACT124700.023.15980712-13
GTAAGCG1453.6379788E-1222.93273494-95
TACATGG124400.022.5659582
GTACTTT130000.022.01350614-15
AGTACTT127500.021.7011912-13
ACATGGG123650.021.435163
AGAGTAC201700.020.17062410-11
CTGTACG1908.257211E-619.999684
CATGGGA77250.019.4301094
ACTTTTT151300.018.9929316-17
GTATCAA295750.018.9291321
TACTTTT145400.018.7999414-15
ATACCGT1600.001191750317.8117566
TAGCCCT15300.017.6957954
CATGGGG40800.017.5793764
GTATAGA6250.017.4887161
ATGGGAT30850.017.2444575
ATGGGAG30050.017.2293425
Produced by FastQC (version 0.11.5)