##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937427_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943974 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.240222348652814 33.0 31.0 34.0 31.0 34.0 2 32.42343981966837 34.0 31.0 34.0 31.0 34.0 3 32.48103575459343 34.0 31.0 34.0 31.0 34.0 4 36.1113960371898 37.0 35.0 37.0 35.0 37.0 5 36.05390504193986 37.0 35.0 37.0 35.0 37.0 6 36.1103615583336 37.0 35.0 37.0 35.0 37.0 7 36.05462058648933 37.0 35.0 37.0 35.0 37.0 8 36.04204120013951 37.0 35.0 37.0 35.0 37.0 9 37.731113687734506 39.0 37.0 39.0 35.0 39.0 10-11 37.65452752968918 39.0 37.0 39.0 35.0 39.0 12-13 37.5589768690322 39.0 37.0 39.0 35.0 39.0 14-15 38.72673708598983 40.0 38.0 41.0 35.0 41.0 16-17 38.70843514367991 40.0 38.0 41.0 34.5 41.0 18-19 38.75665260955137 40.0 38.0 41.0 35.0 41.0 20-21 38.67563840874415 40.0 38.0 41.0 34.0 41.0 22-23 38.615537039075626 40.0 38.0 41.0 34.0 41.0 24-25 38.625629766653255 40.0 38.0 41.0 34.0 41.0 26-27 38.45876976749689 40.0 38.0 41.0 34.0 41.0 28-29 38.31493862572236 40.0 38.0 41.0 34.0 41.0 30-31 38.30883720667046 40.0 38.0 41.0 34.0 41.0 32-33 38.14075548335523 40.0 37.0 41.0 34.0 41.0 34-35 37.972536412524036 40.0 37.0 41.0 33.5 41.0 36-37 37.77095681320841 40.0 37.0 41.0 33.0 41.0 38-39 37.62935821158102 40.0 37.0 41.0 33.0 41.0 40-41 37.43506883322514 40.0 37.0 41.0 33.0 41.0 42-43 37.1739159062827 40.0 36.0 41.0 32.5 41.0 44-45 36.7781094294471 40.0 36.0 41.0 31.0 41.0 46-47 36.29336220546159 39.5 36.0 41.0 30.5 41.0 48-49 35.86020003353954 39.0 35.0 41.0 29.0 41.0 50-51 35.56446382513346 39.0 35.0 41.0 27.5 41.0 52-53 35.336110719587815 39.0 35.0 40.0 27.0 41.0 54-55 35.1298407283225 39.0 35.0 40.0 26.5 41.0 56-57 34.82446910298183 38.0 34.5 40.0 26.0 41.0 58-59 34.57478340759701 38.0 34.0 40.0 25.0 41.0 60-61 34.210101575432596 38.0 34.0 40.0 24.0 41.0 62-63 33.86559208096405 37.5 34.0 40.0 23.0 41.0 64-65 33.47372418561154 37.0 33.0 39.5 22.0 41.0 66-67 33.03321057791925 36.0 33.0 39.0 20.5 40.5 68-69 32.58157285025417 36.0 32.0 39.0 19.5 40.0 70-71 32.12768200603506 35.0 31.5 38.5 18.0 40.0 72-73 31.657966361690022 35.0 31.0 37.5 15.5 39.5 74-75 31.183062633553742 35.0 31.0 37.0 11.5 39.0 76-77 29.873475674057367 33.5 29.5 35.5 8.0 38.0 78-79 30.51083707909674 34.0 30.5 36.0 7.5 38.5 80-81 30.30802443859846 34.0 30.5 36.0 6.5 37.5 82-83 29.90481148410421 34.0 30.0 35.0 2.0 37.0 84-85 29.4759526104773 34.0 29.5 35.0 2.0 36.5 86-87 29.130834311570013 34.0 29.0 35.0 2.0 36.0 88-89 28.688826856737798 34.0 29.0 35.0 2.0 36.0 90-91 28.152178475638046 33.5 28.5 35.0 2.0 35.0 92-93 27.67799620776821 33.0 27.0 35.0 2.0 35.0 94-95 27.199989043063333 33.0 26.5 35.0 2.0 35.0 96-97 26.556278273269086 33.0 25.0 34.5 2.0 35.0 98-99 25.64701688397067 32.0 24.0 34.0 2.0 35.0 100-101 22.76163672970935 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1393254803778987 1101 2 0.1689104163608306 1101 3 0.14238254014334473 1101 4 0.12057177521239737 1101 5 0.05995409174108346 1101 6 0.13337082196072458 1101 7 0.10368842804285805 1101 8 0.08498735310093508 1101 9 0.1571000629004189 1101 10-11 0.17501884709228221 1101 12-13 0.21233257389551596 1101 14-15 0.25403277777125766 1101 16-17 0.26026295839510993 1101 18-19 0.11220303485546168 1101 20-21 0.18070727798148312 1101 22-23 0.17023552711044232 1101 24-25 0.2477062222836537 1101 26-27 0.13312013926914545 1101 28-29 0.05454882440729136 1101 30-31 0.24296522784172225 1101 32-33 0.1628758844377458 1101 34-35 0.3755372565753632 1101 36-37 0.40962924424511726 1101 38-39 0.21766293348063215 1101 40-41 0.3005472883690459 1101 42-43 0.32061342810747817 1101 44-45 0.31009186514987874 1101 46-47 0.4069480707531312 1101 48-49 0.3013302953753012 1101 50-51 0.3431612101688941 1101 52-53 0.27275784636373857 1101 54-55 0.13814269586809047 1101 56-57 0.2666356923275117 1101 58-59 0.31320904628555724 1101 60-61 0.276349399873304 1101 62-63 0.16269274571239123 1101 64-65 0.31096799077150905 1101 66-67 0.26094437809155835 1101 68-69 0.25426871570150666 1101 70-71 0.24326940147012266 1101 72-73 0.307982533878274 1101 74-75 0.2475323181386635 1101 76-77 0.2780118414100592 1101 78-79 0.34757216002163105 1101 80-81 0.3900443091250274 1101 82-83 0.2649906690417083 1101 84-85 0.3188592346934911 1101 86-87 0.3550275777921428 1101 88-89 0.5178798913095122 1101 90-91 0.720982170106435 1101 92-93 0.7140872464902372 1101 94-95 0.8539611645827563 1101 96-97 1.0014440276919707 1101 98-99 1.4249277708951773 1101 100-101 1.866905257764028 1102 1 0.22329564009084635 1102 2 0.25079157857081924 1102 3 0.21970649442489787 1102 4 0.1200084998772013 1102 5 0.10311340108568601 1102 6 0.18013376287890281 1102 7 0.12507071458589536 1102 8 0.12189962851152814 1102 9 0.1517777762686734 1102 10-11 0.055185345959081644 1102 12-13 0.16899363737709194 1102 14-15 0.21299129498220282 1102 16-17 0.36678076366150947 1102 18-19 0.24595209791124972 1102 20-21 0.19455298489202733 1102 22-23 0.15738996656277493 1102 24-25 0.048953189689086685 1102 26-27 0.12876695232826307 1102 28-29 -0.011990846586691362 1102 30-31 0.11436215067300282 1102 32-33 0.13115727373196506 1102 34-35 0.21505035605333944 1102 36-37 0.23841239211154175 1102 38-39 0.2248402587654823 1102 40-41 0.20063466257457918 1102 42-43 0.3589682649572197 1102 44-45 0.36374605844645913 1102 46-47 0.4184275777319826 1102 48-49 0.3444940399587608 1102 50-51 0.3855011091341609 1102 52-53 0.4156845274301446 1102 54-55 0.5949589927097563 1102 56-57 0.5508491248919185 1102 58-59 0.6517796575058199 1102 60-61 0.7340516356772113 1102 62-63 0.6566475151465028 1102 64-65 0.5479073211391494 1102 66-67 0.6663850856008722 1102 68-69 0.5950214644407836 1102 70-71 0.6567201503602078 1102 72-73 0.7778362153482874 1102 74-75 0.7285561486770575 1102 76-77 0.5487731945127301 1102 78-79 0.6447120962428947 1102 80-81 0.6910429031543082 1102 82-83 0.6260213298479442 1102 84-85 0.7488434452431498 1102 86-87 0.9076417075761043 1102 88-89 0.952887009867883 1102 90-91 1.117200907216521 1102 92-93 1.1391272745209484 1102 94-95 1.0299758363529037 1102 96-97 1.235527064233903 1102 98-99 1.5270125549212175 1102 100-101 2.2201432038936204 1103 1 0.13736499480090458 1103 2 0.1689778381515552 1103 3 0.13938402167152475 1103 4 0.15191677864478237 1103 5 0.1409421099257031 1103 6 0.07502601816688781 1103 7 0.11365065432789834 1103 8 0.14281204593776664 1103 9 0.30872072514001303 1103 10-11 0.19753750629176636 1103 12-13 0.19378786011414917 1103 14-15 0.1878289573198657 1103 16-17 0.22178744388804006 1103 18-19 0.30066401427517775 1103 20-21 0.399850644728005 1103 22-23 0.19865600091926439 1103 24-25 0.21567933937691208 1103 26-27 0.09455990971983397 1103 28-29 0.14044068335740434 1103 30-31 0.039817462042258 1103 32-33 0.039315602351983614 1103 34-35 0.10299386145916145 1103 36-37 0.1380555846253415 1103 38-39 0.20060325031050752 1103 40-41 0.19322930969184426 1103 42-43 0.21154883102710897 1103 44-45 0.12393023147610194 1103 46-47 0.08310479226786782 1103 48-49 0.1911749100378728 1103 50-51 0.08522798576115775 1103 52-53 0.12031586412806661 1103 54-55 0.06738549662181725 1103 56-57 0.09015018013204923 1103 58-59 0.08793182889669282 1103 60-61 0.13292245532378644 1103 62-63 0.28366166689369265 1103 64-65 0.301153961084907 1103 66-67 0.36212150126504383 1103 68-69 0.3609183868232648 1103 70-71 0.40984215921399425 1103 72-73 0.3076986180907184 1103 74-75 0.31705272154863096 1103 76-77 0.28271867044941246 1103 78-79 0.18523340317437587 1103 80-81 0.38022984163458773 1103 82-83 0.4899058226583186 1103 84-85 0.4848807974080138 1103 86-87 0.5700288824475734 1103 88-89 0.668339497776401 1103 90-91 0.8824192699537292 1103 92-93 0.6668363918971494 1103 94-95 0.8185949329179785 1103 96-97 0.984175949895981 1103 98-99 1.2886987592110657 1103 100-101 1.8784941442012197 1104 1 0.16494435988027334 1104 2 0.1584619651356789 1104 3 0.18503878357628167 1104 4 0.1181866199146171 1104 5 0.09429528452887581 1104 6 0.12119665308752303 1104 7 0.14478160670884677 1104 8 0.13116522054093593 1104 9 0.13576437593366109 1104 10-11 0.10814266502192993 1104 12-13 0.14316286011415258 1104 14-15 0.1186622906532051 1104 16-17 0.16510093595153563 1104 18-19 0.2068346491958124 1104 20-21 0.20434469234704977 1104 22-23 0.18881473107798996 1104 24-25 0.05106624413881633 1104 26-27 0.14897459225952048 1104 28-29 0.10139306430978223 1104 30-31 0.14476785886765242 1104 32-33 0.21759933251070862 1104 34-35 0.11537481384011272 1104 36-37 0.21093256875231958 1104 38-39 0.22323221856446906 1104 40-41 0.21457851604105116 1104 42-43 0.17344367229694768 1104 44-45 0.2572040409999161 1104 46-47 0.14075360179167262 1104 48-49 0.16024237035532707 1104 50-51 0.06599187465004519 1104 52-53 0.07366903873123931 1104 54-55 0.2131097029710176 1104 56-57 0.1643862912431544 1104 58-59 0.16313024159510547 1104 60-61 0.2641823759587041 1104 62-63 0.31924698435400956 1104 64-65 0.24882261187856614 1104 66-67 0.19205205682060011 1104 68-69 0.3450552915851617 1104 70-71 0.3739790639759022 1104 72-73 0.2937006022177009 1104 74-75 0.3623007374216449 1104 76-77 0.3209131148938553 1104 78-79 0.36667189523786803 1104 80-81 0.42204531782506294 1104 82-83 0.28341772742022187 1104 84-85 0.45368040058261627 1104 86-87 0.5893046760983687 1104 88-89 0.49204981523671876 1104 90-91 0.8022010159854744 1104 92-93 0.8926895665003229 1104 94-95 0.9073449329179795 1104 96-97 1.123789045134071 1104 98-99 1.3193832830205885 1104 100-101 1.9438810489631244 1105 1 0.12337935310294768 1105 2 0.07020340459526864 1105 3 0.045463199726320624 1105 4 0.08239456160739422 1105 5 0.10379065150441136 1105 6 0.02078912470052785 1105 7 0.021215844426336616 1105 8 0.08548137724159943 1105 9 0.18677075328628234 1105 10-11 0.12291134710978469 1105 12-13 0.17129665119475135 1105 14-15 0.21803944328746638 1105 16-17 0.25073389375435085 1105 18-19 0.25698752789278956 1105 20-21 0.2930386683042059 1105 22-23 0.24249659673139234 1105 24-25 0.18891827622557145 1105 26-27 0.059036699230162526 1105 28-29 0.1002239200305084 1105 30-31 0.19008394890609281 1105 32-33 0.2448422940910504 1105 34-35 0.2794793179842756 1105 36-37 0.2993631178268217 1105 38-39 0.2653414112849575 1105 40-41 0.2039193377640487 1105 42-43 -0.07246082295892364 1105 44-45 -0.12270818707376918 1105 46-47 0.09233160770239124 1105 48-49 0.17942453598873698 1105 50-51 0.23341937948305969 1105 52-53 0.1910849887790178 1105 54-55 0.21164335628302666 1105 56-57 0.2367807982760226 1105 58-59 0.1663316948315554 1105 60-61 0.2593502536557466 1105 62-63 0.20363146983275726 1105 64-65 0.18773501771816825 1105 66-67 0.2585032934048286 1105 68-69 0.09990419054745558 1105 70-71 0.1270794128415318 1105 72-73 0.20328462994918794 1105 74-75 0.07350587930107721 1105 76-77 0.15635741860694097 1105 78-79 0.24969102729573578 1105 80-81 0.2441205128120636 1105 82-83 0.2868978287468025 1105 84-85 0.2807463739621703 1105 86-87 0.41535505051785293 1105 88-89 0.6247537460857728 1105 90-91 0.7327614230694479 1105 92-93 0.6625619083442018 1105 94-95 0.8050881944783193 1105 96-97 1.0444875541223624 1105 98-99 1.126993220135894 1105 100-101 1.7038873818742637 1106 1 -0.014526568393051775 1106 2 0.09069879018687743 1106 3 0.07062138460798906 1106 4 0.06768372959137281 1106 5 -0.008826264580697796 1106 6 -0.019191661193005416 1106 7 -0.09543847261302574 1106 8 -0.17683410548234235 1106 9 -0.15904963241327863 1106 10-11 -0.1595177284767857 1106 12-13 -0.39839830327336045 1106 14-15 -0.1476510566781144 1106 16-17 -0.04982868532539442 1106 18-19 -0.016166880212900026 1106 20-21 0.07689063941394636 1106 22-23 0.1099945791740069 1106 24-25 0.20363241050738168 1106 26-27 0.19380635730086482 1106 28-29 0.1604623283790474 1106 30-31 0.048939774776101785 1106 32-33 -0.06473508955425444 1106 34-35 -0.25113878234414955 1106 36-37 0.1212355990553462 1106 38-39 0.20317778198309355 1106 40-41 0.07038625122004305 1106 42-43 0.05608203194129402 1106 44-45 0.0010096764821980742 1106 46-47 0.23765162392921724 1106 48-49 0.26784651271994875 1106 50-51 0.34390773124854235 1106 52-53 0.29579958283783014 1106 54-55 0.4345393112868621 1106 56-57 0.2995764304452777 1106 58-59 0.45261426057013665 1106 60-61 0.5078990983580596 1106 62-63 0.44023474908428994 1106 64-65 0.36818453737821244 1106 66-67 0.3517662647683437 1106 68-69 0.47434048742724855 1106 70-71 0.5546625022940717 1106 72-73 0.5133934243656171 1106 74-75 0.49556758035914683 1106 76-77 0.33183931444280645 1106 78-79 0.43215365898310765 1106 80-81 0.4006634063233925 1106 82-83 0.3841327907999563 1106 84-85 0.5424522076178988 1106 86-87 0.48297072063267876 1106 88-89 0.6033836410915043 1106 90-91 0.5777042951414515 1106 92-93 0.504746880443868 1106 94-95 0.09749087480044949 1106 96-97 0.3352882545075353 1106 98-99 0.35156126471953186 1106 100-101 0.8420414813451096 1107 1 0.12705340126706233 1107 2 0.08764738627456836 1107 3 0.08679720289165971 1107 4 0.04862195449440776 1107 5 1.909981221146495E-4 1107 6 0.06491615543038876 1107 7 0.009402477839657308 1107 8 0.05357802667163014 1107 9 0.16027883707439372 1107 10-11 0.01963390524075237 1107 12-13 0.04434248756377457 1107 14-15 0.06832113237699389 1107 16-17 0.08119261935295441 1107 18-19 0.013363052122663532 1107 20-21 0.09686817871687481 1107 22-23 0.10070091162874206 1107 24-25 0.01891138413374449 1107 26-27 0.06934079496939916 1107 28-29 0.14314421266054467 1107 30-31 0.07463790480168342 1107 32-33 0.12436052171737089 1107 34-35 0.12094910463252972 1107 36-37 0.19870442204041439 1107 38-39 0.15771598007683707 1107 40-41 0.12692311359892727 1107 42-43 0.2886125202530039 1107 44-45 0.20810102412665543 1107 46-47 0.1511745098543713 1107 48-49 0.2737260857028403 1107 50-51 0.21229614720734702 1107 52-53 0.17048527952712078 1107 54-55 0.221567335175628 1107 56-57 0.11650802796499704 1107 58-59 0.2833452544058517 1107 60-61 0.39353953035706013 1107 62-63 0.3810306789273312 1107 64-65 0.4560191356650023 1107 66-67 0.3629706220169169 1107 68-69 0.3002884241013888 1107 70-71 0.38409551482187254 1107 72-73 0.4002700918652309 1107 74-75 0.4371447002127731 1107 76-77 0.4114539361666196 1107 78-79 0.5357024332892486 1107 80-81 0.5956447915457197 1107 82-83 0.5924963923326274 1107 84-85 0.5887806937294471 1107 86-87 0.7815246285485671 1107 88-89 0.9763554958220908 1107 90-91 1.0538320203018898 1107 92-93 1.0508195436487213 1107 94-95 0.9892930897861554 1107 96-97 1.3106065554634618 1107 98-99 1.584027236578745 1107 100-101 2.19909571553746 1108 1 0.04129236600143571 1108 2 0.06523125130004814 1108 3 0.04983669517948641 1108 4 0.10799218297159996 1108 5 -0.01444897688079294 1108 6 0.047119778481352625 1108 7 -0.001990379968617617 1108 8 0.005975734538594679 1108 9 0.02408195627392473 1108 10-11 -0.020410310043338598 1108 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2316 72-73 0.2402425104939283 2316 74-75 0.22211535699641516 2316 76-77 0.18063899786697135 2316 78-79 0.159919127710193 2316 80-81 0.3221771048572215 2316 82-83 0.4737102946316085 2316 84-85 0.6641794341443834 2316 86-87 0.6369175736559178 2316 88-89 0.6239905989302699 2316 90-91 0.8450353157570447 2316 92-93 0.9910738574860929 2316 94-95 1.059862943812373 2316 96-97 1.2462657627583944 2316 98-99 1.2054121004516212 2316 100-101 1.706050263512413 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 15.0 5 42.0 6 93.0 7 203.0 8 543.0 9 923.0 10 1753.0 11 3173.0 12 5392.0 13 7575.0 14 9725.0 15 13693.0 16 19781.0 17 28728.0 18 24872.0 19 13267.0 20 10290.0 21 10035.0 22 10373.0 23 11011.0 24 12722.0 25 14953.0 26 18462.0 27 22285.0 28 27428.0 29 34554.0 30 43830.0 31 55353.0 32 71333.0 33 94408.0 34 131848.0 35 189770.0 36 289248.0 37 422717.0 38 318865.0 39 24698.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.145737353276594 20.578278555224816 15.802382981526542 20.473601109972044 2 15.207250714258524 22.458890911092432 39.712979700345784 22.620878674303256 3 19.473717241074212 26.814298956673287 31.067905229185165 22.644078573067336 4 12.238178082628677 18.321644219521456 39.09218950459214 30.34798819325773 5 12.226569030661718 39.47524505454087 34.30989914890005 13.988286765897358 6 28.98104604279687 38.98905026507556 17.56566703052613 14.46423666160144 7 26.259559027024025 35.20180825463715 21.25074718077505 17.287885537563774 8 26.027097070228304 34.13445858843791 20.137872214340316 19.700572126993467 9 26.623195577718633 17.09709080471241 21.261704117441898 35.018009500127064 10-11 24.183168087639032 27.800937666861795 27.417470604030715 20.598423641468454 12-13 26.2993411660851 25.773025105857382 27.795491409231698 20.132142318825817 14-15 22.74117349306112 26.778418847165653 28.095643254487975 22.384764405285257 16-17 20.35716527072893 29.8854562869668 28.403749227098718 21.35362921520555 18-19 20.20009012466216 28.31241570103304 31.781983709658668 19.70551046464613 20-21 21.97515908167307 26.48927195378529 31.69328744785156 19.842281516690072 22-23 21.11918023320863 26.824908338230962 31.727768082542536 20.328143346017875 24-25 21.16192487290844 26.952824692736137 31.912591356102006 19.97265907825342 26-27 21.037096100054654 26.781107931710768 32.37995048709127 19.8018454811433 28-29 20.290297182809137 27.88701811225809 31.94225848029057 19.880426224642207 30-31 20.897092494787724 26.962492714647198 31.725017219924805 20.415397570640273 32-33 20.666327498020802 27.292822056318798 31.644074571147996 20.3967758745124 34-35 21.345064285839214 27.117466591631366 31.629641137175703 19.907827985353713 36-37 21.18879192540433 26.704916502205407 31.851205790613918 20.255085781776348 38-39 20.621839912385614 27.597528360733403 31.66422578914419 20.11640593773679 40-41 21.026490066225165 27.515800108437666 31.29962129123126 20.158088534105907 42-43 20.715203285116708 27.719921370390015 30.662898440526455 20.901976903966823 44-45 21.201132319670943 27.253579523182925 30.556864443660253 20.98842371348588 46-47 21.749879370814632 27.96794092925111 29.903923612147075 20.378256087787182 48-49 21.50198510885434 28.12200677581079 29.48123277368936 20.894775341645516 50-51 21.033949013721376 28.382015397325272 29.78061949388212 20.80341609507123 52-53 21.587254147240177 28.989155459166494 28.510110863136767 20.913479530456563 54-55 21.22453798250388 28.374942257458173 28.842283898858728 21.558235861179213 56-57 21.74350068467994 28.459099761622326 28.468204821669424 21.32919473202831 58-59 22.2348138401028 28.1588128236283 28.51931147227278 21.087061863996123 60-61 21.648463405374763 28.502207334048705 29.325083565932463 20.524245694644065 62-63 21.3946019854175 28.426126069587347 29.208955469569037 20.970316475426113 64-65 21.195448087268655 29.01471418856425 28.82289063536858 20.966947088798513 66-67 21.519886582845242 28.4660957399636 28.383224261229834 21.630793415961325 68-69 21.57515995584303 28.54222844544217 28.472242941520825 21.410368657193978 70-71 21.885580774228462 28.42808082824153 28.46799905760056 21.218339339929443 72-73 21.69936745244062 28.03963241098281 29.03242317439439 21.22857696218218 74-75 21.21293803312184 29.215591360789805 28.314524782738864 21.256945823349486 76-77 21.15100304839468 28.372524529649056 28.697786081501093 21.77868634045517 78-79 21.204656029350186 28.534846659471786 28.559872714347 21.700624596831027 80-81 21.497201094253317 28.622399270772142 28.34531223154219 21.53508740343235 82-83 21.353500342341203 28.48078182397129 28.633973724862617 21.531744108824892 84-85 21.086709995558056 28.555298092535963 28.469802880036376 21.8881890318696 86-87 20.941560946802788 28.565454064714856 28.34639249290371 22.146592495578645 88-89 20.93850020627848 28.40508669354631 28.078359072703645 22.578054027471563 90-91 21.14538963399153 27.716832242967453 28.422009536639305 22.715768586401712 92-93 21.389095441571627 28.045110248548248 28.554254395113865 22.01153991476626 94-95 20.979678740559287 27.914802358467757 28.73863024917 22.366888651802956 96-97 20.345333836769424 28.14188872896448 29.121145653182605 22.391631781083493 98-99 20.53926855221013 28.43744992519161 28.12453301021391 22.898748512384344 100-101 20.394953802526185 28.049550321265244 28.59363560922581 22.96186026698276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 698.0 1 510.5 2 358.5 3 411.5 4 399.5 5 449.0 6 754.0 7 1502.5 8 2530.5 9 3227.0 10 3179.5 11 2981.5 12 3196.5 13 3447.5 14 3684.0 15 3983.5 16 4177.0 17 4357.5 18 4682.5 19 5086.5 20 5543.0 21 6175.5 22 7037.0 23 8218.5 24 9769.5 25 11896.5 26 14750.0 27 17912.5 28 20540.0 29 23703.5 30 28057.5 31 31857.5 32 36129.0 33 41830.0 34 48600.0 35 53565.0 36 59070.5 37 64543.5 38 65823.0 39 65940.5 40 66013.0 41 66127.5 42 66554.0 43 68094.0 44 70264.5 45 71122.5 46 70250.5 47 77498.5 48 93620.0 49 89239.5 50 70061.0 51 59156.0 52 52433.5 53 48596.0 54 45114.5 55 40693.5 56 36028.0 57 31899.0 58 29428.5 59 26880.0 60 23051.0 61 18857.0 62 14948.5 63 10719.5 64 6988.5 65 5204.0 66 3854.0 67 2728.5 68 1996.5 69 1392.5 70 1062.0 71 819.5 72 672.5 73 660.0 74 523.0 75 406.0 76 319.5 77 187.0 78 106.5 79 64.0 80 42.5 81 29.0 82 19.0 83 10.5 84 4.0 85 2.5 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04434215683954621 2 0.0 3 0.0 4 0.0 5 9.773793270897657E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00437248646329632 14-15 0.0 16-17 0.0 18-19 0.0 20-21 2.057640688610033E-4 22-23 3.343666118991303E-4 24-25 7.716152582287624E-5 26-27 0.0018775971283566551 28-29 0.003575150696459932 30-31 2.572050860762541E-4 32-33 2.572050860762541E-4 34-35 0.0 36-37 2.572050860762541E-5 38-39 5.144101721525082E-5 40-41 0.0 42-43 0.009105060047099395 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.572050860762541E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.886896635448828E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.6008712050675576E-4 84-85 2.829255946838795E-4 86-87 0.0 88-89 0.0 90-91 0.010674011072164546 92-93 0.001311745938988896 94-95 0.0 96-97 0.0 98-99 3.858076291143812E-4 100-101 0.013014577355458459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943974.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.72163490068359 #Duplication Level Percentage of deduplicated Percentage of total 1 69.89909961771242 32.65800212225272 2 15.258842641177338 14.2583614977614 3 5.960747610161556 8.354876207312714 4 2.9663907002989562 5.543784930886038 5 1.6850516783326028 3.9364184651920002 6 1.0264526783901713 2.8774528369544625 7 0.6897947260616808 2.255983614322963 8 0.46325942892107097 1.7315390321879565 9 0.33625617586961504 1.4139394453872185 >10 1.541439584510399 12.766959877567713 >50 0.10808182500675427 3.4831091110923396 >100 0.0597184415575266 4.99781667878977 >500 0.0019901830908952227 0.676463021441782 >1k 0.002321880272711093 2.361757378945921 >5k 2.211314545439136E-4 0.6801307476366937 >10k+ 3.316971818158704E-4 2.003405032268438 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14162 0.7285076858023821 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13211 0.6795872784306786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11409 0.5868905654087966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7817 0.40211443157161564 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5349 0.27515800108437666 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4586 0.23590850494914026 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3636 0.187039538594652 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3619 0.1861650413019927 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3522 0.1811752626321134 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2859 0.1470698682184021 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2826 0.14537231465029882 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2817 0.14490934549536155 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2807 0.14439493532320907 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2628 0.13518699324167915 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2082 0.1071001978421522 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1960 0.10082439374189162 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.0288203443050164E-4 0.0 8 0.0 0.0 0.0 1.0288203443050164E-4 0.0 9 0.0 0.0 0.0 1.0288203443050164E-4 0.0 10-11 0.0 0.0 0.0 1.2860254303812707E-4 0.0 12-13 0.0 0.0 0.0 1.5432305164575247E-4 0.0 14-15 0.0 0.0 0.0 1.5432305164575247E-4 0.0 16-17 0.0 0.0 0.0 1.5432305164575247E-4 0.0 18-19 0.0 0.0 0.0 1.5432305164575247E-4 0.0 20-21 0.0 0.0 0.0 3.0864610329150495E-4 0.0 22-23 0.0 0.0 0.0 6.430127151906353E-4 0.0 24-25 5.144101721525082E-5 0.0 0.0 9.002178012668893E-4 0.0 26-27 5.144101721525082E-5 0.0 0.0 0.001183143395950769 0.0 28-29 5.144101721525082E-5 0.0 0.0 0.002829255946838795 0.0 30-31 5.144101721525082E-5 0.0 0.0 0.005529909350639463 0.0 32-33 5.144101721525082E-5 0.0 0.0 0.008307724280263008 0.0 34-35 5.144101721525082E-5 0.0 0.0 0.01288597481242033 0.0 36-37 1.5432305164575247E-4 0.0 0.0 0.019084617386858054 0.0 38-39 1.5432305164575247E-4 0.0 0.0 0.029269938795477717 0.0 40-41 1.5432305164575247E-4 0.0 0.0 0.04097277021194728 0.0 42-43 1.5432305164575247E-4 0.0 0.0 0.05267560162841684 0.0 44-45 1.5432305164575247E-4 0.0 0.0 0.06180638218412386 0.0 46-47 1.5432305164575247E-4 0.0 0.0 0.07417794682439169 0.0 48-49 1.5432305164575247E-4 0.0 0.0 0.0859322192580765 0.0 50-51 1.5432305164575247E-4 0.0 0.0 0.0985867094930282 0.0 52-53 1.5432305164575247E-4 0.0 0.0 0.12613337421179502 0.0 54-55 1.5432305164575247E-4 0.0 0.0 0.17376775615311726 0.0 56-57 1.5432305164575247E-4 0.0 0.0 0.24758561585700223 0.0 58-59 1.5432305164575247E-4 0.0 0.0 0.33943355209483256 0.0 60-61 1.5432305164575247E-4 0.0 0.0 0.45517584082914686 0.0 62-63 1.5432305164575247E-4 0.0 0.0 0.5265245317066998 0.0 64-65 1.5432305164575247E-4 0.0 0.0 0.621124562365546 0.0 66-67 1.5432305164575247E-4 0.0 0.0 0.7902369064606831 0.0 68-69 1.5432305164575247E-4 0.0 0.0 1.0042829790933419 0.0 70-71 1.5432305164575247E-4 0.0 0.0 1.2358961591050086 0.0 72-73 1.5432305164575247E-4 0.0 0.0 1.517047038694962 0.0 74-75 1.5432305164575247E-4 0.0 0.0 1.7993553411722585 0.0 76-77 1.5432305164575247E-4 0.0 0.0 2.0540912584221807 0.0 78-79 1.5432305164575247E-4 0.0 0.0 2.3178036331761636 0.0 80-81 1.5432305164575247E-4 0.0 0.0 2.5925758266314265 0.0 82-83 1.5432305164575247E-4 0.0 0.0 2.9535631649394487 0.0 84-85 1.5432305164575247E-4 0.0 0.0 3.4730145567790514 0.0 86-87 1.5432305164575247E-4 0.0 0.0 4.084056679770408 0.0 88-89 1.5432305164575247E-4 0.0 0.0 4.769431072637802 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTCTA 95 0.0012267905 25.000244 9 GTACATG 12135 0.0 23.419172 1 GAGTACT 12470 0.0 23.159807 12-13 GTAAGCG 145 3.6379788E-12 22.932734 94-95 TACATGG 12440 0.0 22.565958 2 GTACTTT 13000 0.0 22.013506 14-15 AGTACTT 12750 0.0 21.70119 12-13 ACATGGG 12365 0.0 21.43516 3 AGAGTAC 20170 0.0 20.170624 10-11 CTGTACG 190 8.257211E-6 19.99968 4 CATGGGA 7725 0.0 19.430109 4 ACTTTTT 15130 0.0 18.99293 16-17 GTATCAA 29575 0.0 18.929132 1 TACTTTT 14540 0.0 18.79994 14-15 ATACCGT 160 0.0011917503 17.811756 6 TAGCCCT 1530 0.0 17.695795 4 CATGGGG 4080 0.0 17.579376 4 GTATAGA 625 0.0 17.488716 1 ATGGGAT 3085 0.0 17.244457 5 ATGGGAG 3005 0.0 17.229342 5 >>END_MODULE