FastQCFastQC Report
Thu 26 May 2016
SRR937426_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937426_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943873
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139590.7181024686283517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102530.5274521535100287No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92880.47780899266567317No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT73680.3790371078769035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68730.3535724813297988No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57640.29652142912628554No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39640.20392278713681397No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34260.17624608191996083No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31840.16379670894137632No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28000.14404233198362237No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27540.14167592224389147No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25960.13354781922481562No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25350.1304097541351724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23990.12341341229596789No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA20630.10612833245793321No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20410.10499657127806189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112300.026.5019381
TACATGG113600.025.9395222
ACATGGG113000.025.3622343
GAGTACT111300.024.2830112-13
AGTACTT115550.023.53374112-13
CATGGGG38200.022.8983354
GTATCAA242300.022.5645921
GTACTTT121000.022.21744514-15
AGAGTAC178150.021.91707410-11
CATGGGA72750.020.126454
ATGGGAT30300.019.9250475
TACTTTT137350.019.72831514-15
CAACGCA274850.019.6827055
TACTGCG1456.147304E-419.6555637
TCAACGC275600.019.6468984
ACTTTTT139800.019.51772116-17
ATGGGCG1951.0261916E-519.5026515
ATCAACG278250.019.4723453
AACGCAG278100.019.454726
TATCAAC281850.019.3079452
Produced by FastQC (version 0.11.5)