FastQCFastQC Report
Thu 26 May 2016
SRR937426_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937426_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943873
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148830.7656364381829471No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT134830.693615272191136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119760.6160896313699507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80090.41201251316315424No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT55070.28330040079778873No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45360.23334857781346827No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37180.19126763939825286No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36760.18910700441849854No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35650.1833967548291478No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29820.15340508356255783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.14584286113341768No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28320.1456885300634352No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27910.14357933877367504No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26600.13684021538444127No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19490.1002637517986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC700.00836884527.1588821
GTACATG126150.024.9037151
TACATGG125300.024.5270962
TTTACGC1201.7114278E-423.7499623
GAGTACT126600.023.7305912-13
ACATGGG125000.023.6739623
GTACTTT133250.022.59917614-15
AGTACTT130450.021.97429512-13
AGAGTAC203050.020.50362410-11
CATGGGA77350.019.957954
ACTTTTT151250.019.925916-17
CATGGGG42100.019.8574494
TAACGCG1201.4161378E-719.79061776-77
TACTTTT149250.019.142214-15
GTGTAGC14400.018.8131851
GTATCAA304300.018.4614351
ATGGGAT32100.017.9049575
CATGGGT19650.017.6462824
CGTATAG1901.8581092E-417.5103321
GTATAGG6550.017.4148561
Produced by FastQC (version 0.11.5)