##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937426_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943873 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.203748907464636 33.0 31.0 34.0 31.0 34.0 2 32.407500387113764 34.0 31.0 34.0 31.0 34.0 3 32.47902203487573 34.0 31.0 34.0 31.0 34.0 4 36.09959961376077 37.0 35.0 37.0 35.0 37.0 5 35.97850631188354 37.0 35.0 37.0 35.0 37.0 6 36.08519023619341 37.0 35.0 37.0 35.0 37.0 7 36.050513588079056 37.0 35.0 37.0 35.0 37.0 8 36.03299238170395 37.0 35.0 37.0 35.0 37.0 9 37.730143378708384 39.0 37.0 39.0 35.0 39.0 10-11 37.65488923401889 39.0 37.0 39.0 35.0 39.0 12-13 37.58212753610961 39.0 37.0 39.0 35.0 39.0 14-15 38.73826222186326 40.0 38.0 41.0 35.0 41.0 16-17 38.75137933393797 40.0 38.0 41.0 35.0 41.0 18-19 38.82327472010775 40.0 38.0 41.0 35.0 41.0 20-21 38.76025517099111 40.0 38.0 41.0 35.0 41.0 22-23 38.6244641496641 40.0 38.0 41.0 34.0 41.0 24-25 38.47000858595186 40.0 38.0 41.0 34.0 41.0 26-27 38.25170111421888 40.0 38.0 41.0 34.0 41.0 28-29 38.241123777119185 40.0 38.0 41.0 34.0 41.0 30-31 38.30064438366087 40.0 38.0 41.0 34.0 41.0 32-33 38.19179159338084 40.0 37.0 41.0 34.0 41.0 34-35 38.03262147269909 40.0 37.0 41.0 34.0 41.0 36-37 37.84928593586103 40.0 37.0 41.0 33.0 41.0 38-39 37.68539559940387 40.0 37.0 41.0 33.0 41.0 40-41 37.51415961845244 40.0 37.0 41.0 33.0 41.0 42-43 37.25222069548782 40.0 36.5 41.0 33.0 41.0 44-45 36.86204834369323 40.0 36.0 41.0 31.5 41.0 46-47 36.394520115254444 40.0 36.0 41.0 30.5 41.0 48-49 35.964365213159496 39.5 36.0 41.0 29.5 41.0 50-51 35.702505513477476 39.0 35.0 41.0 28.0 41.0 52-53 35.48692198512968 39.0 35.0 41.0 27.5 41.0 54-55 35.28914774782098 39.0 35.0 40.5 27.0 41.0 56-57 35.0395977514992 39.0 35.0 40.0 26.0 41.0 58-59 34.80332742931252 38.0 35.0 40.0 26.0 41.0 60-61 34.462698180385246 38.0 34.0 40.0 25.0 41.0 62-63 34.132754557525104 38.0 34.0 40.0 23.5 41.0 64-65 33.81009690447884 37.0 34.0 40.0 23.0 41.0 66-67 33.42651834764925 37.0 33.0 39.5 22.0 41.0 68-69 32.99001606586438 36.0 33.0 39.0 21.0 40.0 70-71 32.59469342904603 35.5 32.5 39.0 20.0 40.0 72-73 32.16777202008568 35.0 32.0 38.0 18.5 40.0 74-75 31.7361370830296 35.0 32.0 37.0 17.5 39.0 76-77 30.41847744168472 34.0 30.0 36.0 13.5 38.5 78-79 31.013865617764125 35.0 31.0 36.0 12.5 39.0 80-81 30.821539781662693 35.0 31.0 36.0 9.0 37.5 82-83 30.48925675700007 34.5 31.0 35.5 7.0 37.0 84-85 30.154467652979385 34.0 31.0 35.0 3.5 37.0 86-87 29.856684310137545 34.0 31.0 35.0 2.0 36.0 88-89 29.499001477977213 34.0 30.0 35.0 2.0 36.0 90-91 29.071970751175616 34.0 29.5 35.0 2.0 35.5 92-93 28.699484482782566 34.0 29.0 35.0 2.0 35.0 94-95 28.323170032198604 34.0 29.0 35.0 2.0 35.0 96-97 27.939737061011705 34.0 29.0 35.0 2.0 35.0 98-99 27.384922008793787 33.5 28.0 35.0 2.0 35.0 100-101 25.855564381006374 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.055045718735335925 1101 2 0.09607217598198758 1101 3 0.06898374873696866 1101 4 0.0254735964077355 1101 5 -0.07302029548329614 1101 6 -0.007602458544809565 1101 7 0.04749710888019365 1101 8 -0.052546272349069056 1101 9 0.010628581376728619 1101 10-11 0.08798653329838402 1101 12-13 0.05856836655522102 1101 14-15 0.11419740224287978 1101 16-17 0.09082444232447529 1101 18-19 0.1238053413039708 1101 20-21 0.0676377509844599 1101 22-23 0.022355344664362065 1101 24-25 0.06883366285175185 1101 26-27 0.14139566307127183 1101 28-29 0.1005075243925404 1101 30-31 0.1426929666690242 1101 32-33 0.13613817386636384 1101 34-35 0.1175320287696735 1101 36-37 0.0923915280327634 1101 38-39 0.2320815824875737 1101 40-41 0.08031861604617063 1101 42-43 0.04336293028296012 1101 44-45 -0.03248726526340562 1101 46-47 -0.16442840374432421 1101 48-49 -0.19665161734059922 1101 50-51 -0.12146679195813448 1101 52-53 -0.004309291270864435 1101 54-55 -0.023071261230164453 1101 56-57 -0.08187302422746967 1101 58-59 -0.09440969795991094 1101 60-61 0.036840712323069624 1101 62-63 0.062313170360681625 1101 64-65 0.10539830116643856 1101 66-67 0.05539141686797677 1101 68-69 -0.012904990680333128 1101 70-71 -0.0842282622522319 1101 72-73 0.032193946738118484 1101 74-75 -0.0390310737858961 1101 76-77 0.11098169947980097 1101 78-79 0.11057842750821933 1101 80-81 0.09315121667335191 1101 82-83 0.06851791520357153 1101 84-85 0.07705351996385446 1101 86-87 -0.08551980253425384 1101 88-89 -0.011459694370540063 1101 90-91 0.16800663621793532 1101 92-93 0.19146214994715294 1101 94-95 0.04743256630898429 1101 96-97 0.11997909316279731 1101 98-99 -0.022544838402787093 1101 100-101 0.40823823120802416 1102 1 0.1260450488211049 1102 2 0.09477149476467162 1102 3 0.03758269065843933 1102 4 0.04886876585744204 1102 5 -0.008860155655931123 1102 6 -0.005739931562153799 1102 7 0.09854898450220873 1102 8 0.09429286277235605 1102 9 0.1899671030993204 1102 10-11 0.0672368358625306 1102 12-13 0.062396250977087675 1102 14-15 0.12959826182831335 1102 16-17 0.1395520188063486 1102 18-19 0.1106311884146649 1102 20-21 0.14754721607310728 1102 22-23 0.1605961443710342 1102 24-25 0.21451767836729374 1102 26-27 0.19874225133316514 1102 28-29 0.03645738282835964 1102 30-31 0.13878154973149748 1102 32-33 0.12599297064526382 1102 34-35 0.1220336870141665 1102 36-37 0.042664318211762975 1102 38-39 0.059591072939227274 1102 40-41 -0.12991287653928651 1102 42-43 0.08383677743776019 1102 44-45 0.028447361583332054 1102 46-47 0.13218012098322873 1102 48-49 0.14585127045841517 1102 50-51 -0.013843278244188184 1102 52-53 -0.053768535398084794 1102 54-55 0.014841749165917406 1102 56-57 -0.0026613175451473126 1102 58-59 -0.026067694337420733 1102 60-61 -0.02546572243748102 1102 62-63 0.12676884436883995 1102 64-65 0.1605578813535118 1102 66-67 0.002394860172003632 1102 68-69 0.10475290093690859 1102 70-71 0.013249496870862743 1102 72-73 9.100547293954264E-4 1102 74-75 -0.10209784741829608 1102 76-77 -0.09401784564016324 1102 78-79 0.0015831058804671727 1102 80-81 0.09598884104169159 1102 82-83 0.1455679768191409 1102 84-85 0.022571848872445344 1102 86-87 -0.020963503548497187 1102 88-89 0.05751528171379405 1102 90-91 0.033287710727286424 1102 92-93 0.08034708735290153 1102 94-95 0.04243329687388808 1102 96-97 0.059084440622221734 1102 98-99 0.19490187860393604 1102 100-101 0.35728449939977835 1103 1 0.07837170977906993 1103 2 0.07105282805667912 1103 3 0.006226355183635235 1103 4 0.06540374543637029 1103 5 -0.042672165121274475 1103 6 0.0011143605901224873 1103 7 0.05223718521354925 1103 8 0.04488260335456573 1103 9 0.01723002734475898 1103 10-11 0.0374352001105791 1103 12-13 0.05255562669054825 1103 14-15 0.039231302453899275 1103 16-17 0.05746531175739733 1103 18-19 0.02306317473321684 1103 20-21 0.0723401236684964 1103 22-23 0.0198880707704987 1103 24-25 0.10276496283389491 1103 26-27 0.03302082265032169 1103 28-29 -0.17491300359084505 1103 30-31 -0.13148135648432202 1103 32-33 0.015340973196963148 1103 34-35 0.058150104031447825 1103 36-37 0.09154088078678058 1103 38-39 0.08893670125406317 1103 40-41 0.2847239898083984 1103 42-43 0.10815102915913144 1103 44-45 0.06153372971605364 1103 46-47 0.1049086073484844 1103 48-49 1.0057707793720283E-4 1103 50-51 -0.15179970856691938 1103 52-53 0.015531427347461602 1103 54-55 0.0019712380587577627 1103 56-57 -0.05036666421715097 1103 58-59 -0.11715624344572717 1103 60-61 -0.18228892869850455 1103 62-63 0.04386219502680433 1103 64-65 -0.0135295922579175 1103 66-67 -0.047610984259165434 1103 68-69 -1.9687645811927723E-4 1103 70-71 -0.025581227941920304 1103 72-73 0.08390350957422754 1103 74-75 0.06241357130369707 1103 76-77 -0.20067071256155344 1103 78-79 0.14755380107201077 1103 80-81 -0.05238222381771962 1103 82-83 -0.10074633446073378 1103 84-85 -0.14443160693869217 1103 86-87 -0.024349931057393803 1103 88-89 -0.3176034048065155 1103 90-91 -0.21653751343247762 1103 92-93 -0.21282548259440404 1103 94-95 -0.210223589638602 1103 96-97 0.02641781811798083 1103 98-99 0.09128578622100392 1103 100-101 0.26109610167945263 1104 1 0.036469207162213024 1104 2 0.11982800879795974 1104 3 0.11499950508857637 1104 4 0.08704581746967932 1104 5 -0.1157585170811899 1104 6 0.05520608585707265 1104 7 0.014846881384876554 1104 8 0.07859364561719673 1104 9 0.024350778644880222 1104 10-11 0.2054872223169113 1104 12-13 0.10592468115964238 1104 14-15 0.19037366891642193 1104 16-17 0.20516871126390157 1104 18-19 0.10729064017589707 1104 20-21 0.056286635728113765 1104 22-23 0.059231425279051564 1104 24-25 0.09042058933324171 1104 26-27 0.14665652214624458 1104 28-29 0.01719149996743141 1104 30-31 0.06609337082964117 1104 32-33 0.12325185858484389 1104 34-35 0.11411068226058774 1104 36-37 0.11684791864379918 1104 38-39 0.07567771559164527 1104 40-41 0.07079356676585036 1104 42-43 0.12559950824982025 1104 44-45 0.0760867954064608 1104 46-47 0.1387947724546379 1104 48-49 -0.08291101445654903 1104 50-51 0.06887073652050191 1104 52-53 0.1997460476451849 1104 54-55 0.09832979714042978 1104 56-57 -0.01332860327482166 1104 58-59 -0.048661347551323786 1104 60-61 -0.12006439766717136 1104 62-63 -0.04088969290467048 1104 64-65 -0.11673297378962388 1104 66-67 -0.09900890813895558 1104 68-69 -0.1338132935204257 1104 70-71 -0.15822942380872718 1104 72-73 -0.08381550592845599 1104 74-75 -0.15949335037938894 1104 76-77 -0.06525502916515435 1104 78-79 -0.04571643618055177 1104 80-81 0.04025681436473505 1104 82-83 0.1619546442299935 1104 84-85 0.017994094619606216 1104 86-87 0.021848547244925243 1104 88-89 0.19639454459538896 1104 90-91 0.2771095007230393 1104 92-93 0.28567115191454917 1104 94-95 0.13351038677888738 1104 96-97 0.06444133923920248 1104 98-99 0.31019827546503365 1104 100-101 0.6120474307735364 1105 1 0.07120918478693739 1105 2 0.1193210522392647 1105 3 0.08112160986799921 1105 4 0.07471037281388959 1105 5 -0.05418806645575103 1105 6 -0.002855815375234272 1105 7 0.006851745092781414 1105 8 0.09171493700254274 1105 9 0.06200543616412091 1105 10-11 0.12444474965017349 1105 12-13 0.09475273810875962 1105 14-15 0.11003386708219409 1105 16-17 0.11107168012024005 1105 18-19 0.12125895902124029 1105 20-21 0.15738944775677055 1105 22-23 0.12818872838975182 1105 24-25 0.22169895482328883 1105 26-27 0.11477570263622994 1105 28-29 -0.031055752184947494 1105 30-31 0.09637633758043052 1105 32-33 0.1288860379633192 1105 34-35 0.14423434421238568 1105 36-37 0.13002056767546577 1105 38-39 0.13739060093681843 1105 40-41 1.1434107259589155E-4 1105 42-43 -0.033319948226122165 1105 44-45 0.05871082732550548 1105 46-47 -0.05964231079835258 1105 48-49 -0.1880175874451524 1105 50-51 -0.15422047273745676 1105 52-53 -0.1957198943341254 1105 54-55 -0.1634758949951376 1105 56-57 -0.17881062735120423 1105 58-59 -0.23496147651133015 1105 60-61 -0.10257076612209204 1105 62-63 -0.03615347154189408 1105 64-65 -0.12384146996726741 1105 66-67 -0.12709374627268488 1105 68-69 -0.15969530504033713 1105 70-71 -0.152291796549072 1105 72-73 -0.14338001871309203 1105 74-75 -0.2555242478227626 1105 76-77 -0.12012891251005087 1105 78-79 -0.152447341546452 1105 80-81 -0.1040035041978804 1105 82-83 -0.08363900852014439 1105 84-85 -0.09567660613145534 1105 86-87 -0.09405103264738912 1105 88-89 -0.04212253700049118 1105 90-91 -0.0800734266713441 1105 92-93 -0.13347845942231373 1105 94-95 -0.15091946967381986 1105 96-97 -0.04806831567798753 1105 98-99 -0.050953284686301004 1105 100-101 0.19165060807145196 1106 1 -0.07687323350603492 1106 2 -0.019804717032180008 1106 3 -0.020713136367831453 1106 4 0.08567319163419995 1106 5 -0.009466197732812986 1106 6 -6.840878746032786E-4 1106 7 -0.010711186651015225 1106 8 0.013883113279142378 1106 9 -0.013494990866036005 1106 10-11 -0.12032993447093787 1106 12-13 -0.16995421003770872 1106 14-15 -0.0724264607854721 1106 16-17 0.07006002517510268 1106 18-19 0.021488361752012963 1106 20-21 -0.14125796167285642 1106 22-23 0.004931472330866882 1106 24-25 0.05935637902175728 1106 26-27 0.1815124425627559 1106 28-29 0.10384806920841072 1106 30-31 -0.03933183152577868 1106 32-33 0.031304154279048646 1106 34-35 0.14346079793244115 1106 36-37 0.09480388525703631 1106 38-39 -0.07230113786975068 1106 40-41 0.02747473301176484 1106 42-43 -0.2580235353927023 1106 44-45 -0.11546584018140749 1106 46-47 -0.035452201636715586 1106 48-49 -0.2815168091427722 1106 50-51 -0.12373724581627954 1106 52-53 -0.0923359039677294 1106 54-55 -0.09514653980275511 1106 56-57 -0.0017836948953444676 1106 58-59 -0.009067617796141292 1106 60-61 0.05437159375234302 1106 62-63 0.032345970974816396 1106 64-65 -0.07214839899872771 1106 66-67 -0.06222321081015991 1106 68-69 0.028388888864640194 1106 70-71 0.041370174484448796 1106 72-73 0.16052809427109338 1106 74-75 -0.10244792073963183 1106 76-77 -0.04927372657924067 1106 78-79 0.2597444544465972 1106 80-81 0.08858471982546945 1106 82-83 0.08583477844129561 1106 84-85 0.10647636873208555 1106 86-87 0.2873440865484902 1106 88-89 0.14678582171571364 1106 90-91 0.16084167937051674 1106 92-93 0.22645179798027826 1106 94-95 -0.027627335521803076 1106 96-97 0.299917213990021 1106 98-99 0.26122467331005694 1106 100-101 0.34829220101284974 1107 1 0.061239896129791305 1107 2 0.07918597711847042 1107 3 0.04013778828490899 1107 4 0.048355123667569444 1107 5 0.006800263512545257 1107 6 0.05714912016901508 1107 7 0.039572592706349496 1107 8 0.053150367849326585 1107 9 -0.011185713564643152 1107 10-11 0.022616532126853883 1107 12-13 0.014214568777802583 1107 14-15 0.0994525105782742 1107 16-17 0.07496234690390935 1107 18-19 0.024400644196198584 1107 20-21 0.09769536328282413 1107 22-23 0.05890654828137798 1107 24-25 0.1406435515979112 1107 26-27 0.26435287321361756 1107 28-29 0.07509756680605761 1107 30-31 0.038006330088897755 1107 32-33 0.08875757916478477 1107 34-35 0.10902993815963669 1107 36-37 0.08279442015562921 1107 38-39 0.10057065149481303 1107 40-41 0.2702289473816819 1107 42-43 0.3467050034341028 1107 44-45 0.06287005643341814 1107 46-47 0.08367605107454779 1107 48-49 0.02638189773532673 1107 50-51 0.03166684224057548 1107 52-53 0.1128125612802151 1107 54-55 0.08018154758969587 1107 56-57 0.07443224112397218 1107 58-59 0.08790808513294479 1107 60-61 0.023353400610155006 1107 62-63 0.17477627495546244 1107 64-65 0.1345029311963657 1107 66-67 0.15012388824585088 1107 68-69 0.14042002790343844 1107 70-71 0.11230131472191829 1107 72-73 0.23102697889505208 1107 74-75 0.21946350037347528 1107 76-77 0.21476656933116445 1107 78-79 0.18970705834266965 1107 80-81 0.18002023655929023 1107 82-83 0.18192321984790993 1107 84-85 0.1656205319725501 1107 86-87 0.2360556247889285 1107 88-89 0.3806082211673605 1107 90-91 0.4534746933761369 1107 92-93 0.4449316917339523 1107 94-95 0.2804577770028729 1107 96-97 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90-91 -0.18711990498907127 2316 92-93 -0.2625704819245449 2316 94-95 -0.3477540266063599 2316 96-97 -0.4302227959424023 2316 98-99 -0.4712088681505264 2316 100-101 -0.5121582336725403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 13.0 5 31.0 6 91.0 7 181.0 8 480.0 9 966.0 10 1811.0 11 3322.0 12 5272.0 13 7596.0 14 9482.0 15 13674.0 16 19370.0 17 28852.0 18 24998.0 19 12449.0 20 9362.0 21 8999.0 22 9108.0 23 9632.0 24 11042.0 25 12806.0 26 15431.0 27 19178.0 28 23808.0 29 29699.0 30 37609.0 31 48292.0 32 62108.0 33 82876.0 34 116125.0 35 169285.0 36 266324.0 37 430201.0 38 408329.0 39 45054.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24442564107315 20.518149542388393 15.840536431360317 20.396888385178137 2 15.262725496984627 22.498074719901968 39.72003315031383 22.51916663279957 3 19.408418142543262 26.873051891764533 31.081094289596077 22.637435676096125 4 12.220975568852047 18.367371085838517 39.01236244586189 30.399290899447546 5 12.2036210898049 39.466184828795434 34.22115596842878 14.10903811297088 6 28.978796454295107 38.979758451298004 17.60367061016846 14.437774484238425 7 26.287365481181123 35.16104189934219 21.298459312928365 17.253133306548317 8 26.09666372237281 34.03447653216028 20.098586687504792 19.77027305796212 9 26.633015634251826 17.10363794342532 21.179264283211918 35.08408213911094 10-11 24.191703881889403 27.796594736384527 27.444385512839574 20.567315868886496 12-13 26.357470311727504 25.77551174746538 27.731776367810983 20.135241572996136 14-15 22.754727289282787 26.722913482516603 28.132264813596365 22.39009441460425 16-17 20.38198997568257 29.843693492321773 28.452115956134993 21.322200575860666 18-19 20.175855109876007 28.26691867215605 31.876002187385698 19.681224030582246 20-21 21.938598869370583 26.412810919231866 31.887834236084355 19.760755975313202 22-23 21.090773465030736 26.72366694961031 31.796589242997147 20.38897034236181 24-25 21.15322486884357 26.929946673169187 32.001139998621305 19.915688459365942 26-27 20.985118608196057 26.677108214320437 32.546350336988134 19.791422840495372 28-29 20.250459467210955 27.82623458100063 32.09294549027627 19.830360461512146 30-31 20.846563024086095 26.879533505499364 31.88071671828218 20.393186752132355 32-33 20.59437524982342 27.208824856356355 31.77705024968195 20.419749644138275 34-35 21.355536086976876 27.03149845694652 31.736229681671592 19.876735774405017 36-37 21.151384764809134 26.602449343268162 32.02768912292399 20.21847676899872 38-39 20.58948961555003 27.482781752670448 31.839147514131618 20.088581117647905 40-41 20.962362748164487 27.499135745563496 31.422567946860696 20.11593355941132 42-43 20.654325551387316 27.669009919659533 30.803602780416696 20.873061748536465 44-45 21.175148788012386 27.176826881179995 30.683254513026313 20.964769817781306 46-47 21.71363561302616 27.91697297097084 30.023360579626345 20.346030836376656 48-49 21.507012032164653 28.051266723700568 29.60694963096869 20.834771613166087 50-51 20.988588246248597 28.328265272474074 29.883613795757235 20.79953268552009 52-53 21.59275054401786 28.882281222509736 28.626374191689774 20.89859404178263 54-55 21.220367791517244 28.323198069009653 28.919919151096806 21.536514988376297 56-57 21.738482915293332 28.37862864497835 28.550450569558816 21.332437870169503 58-59 22.174905459358712 28.149549893434394 28.627718991930028 21.04782565527686 60-61 21.653729436027973 28.493785345030258 29.365961665191094 20.486523553750683 62-63 21.35455865686699 28.380943611028087 29.316472835416718 20.948024896688207 64-65 21.217949938087518 28.956186952542684 28.842702172415585 20.983160936954214 66-67 21.55596070319409 28.39833157824611 28.435396756886895 21.61031096167291 68-69 21.555806372124106 28.465928586898425 28.56835297367678 21.40991206730069 70-71 21.912362587473563 28.41299302989444 28.503250983989183 21.171393398642813 72-73 21.74465615809263 28.01980890726915 29.07705390218394 21.15848103245428 74-75 21.16894982336809 29.260090551183126 28.378782976048335 21.192176649400448 76-77 21.186029128446147 28.321731923844816 28.7590290106401 21.733209937068935 78-79 21.230888026121047 28.55387157494342 28.550090463728857 21.665149935206674 80-81 21.518329643963366 28.5936632691539 28.400440769535866 21.487566317346865 82-83 21.420042760193102 28.41857763711865 28.69622051748476 21.465159085203492 84-85 21.06326418013527 28.540143450766447 28.55117812510851 21.84541424398977 86-87 20.93007104888025 28.58908478074442 28.380069068298187 22.10077510207714 88-89 20.949645372923026 28.428992017482624 28.107494676864174 22.51386793273017 90-91 21.126137029827667 27.764505659393052 28.45004837384895 22.659308936930326 92-93 21.36747322033968 28.110412145943105 28.569069453747403 21.953045179969813 94-95 20.95054563749792 27.999411484186464 28.72129506402939 22.32874781428622 96-97 20.342841327597018 28.1785126909011 29.181921864237015 22.296724117264862 98-99 20.56969709441106 28.508776442505557 28.105013787142973 22.816512675940405 100-101 20.182960177439625 28.34781727340407 28.480120104113553 22.989102445042747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 720.0 1 521.0 2 371.5 3 448.5 4 452.5 5 468.0 6 781.0 7 1630.5 8 2691.0 9 3385.0 10 3373.0 11 3197.5 12 3350.5 13 3606.5 14 3853.5 15 4042.5 16 4239.0 17 4556.0 18 4907.0 19 5230.0 20 5775.5 21 6419.5 22 7213.0 23 8479.5 24 9979.0 25 11972.5 26 14727.0 27 17746.0 28 20477.0 29 23788.0 30 28067.0 31 31875.0 32 36142.5 33 41648.5 34 48375.0 35 53248.5 36 58521.5 37 63971.0 38 65353.5 39 65543.5 40 66184.0 41 66563.5 42 66112.5 43 67455.5 44 69973.0 45 70656.0 46 70013.5 47 77875.0 48 94454.0 49 89423.5 50 69509.0 51 58973.5 52 52513.5 53 48539.5 54 44792.5 55 40554.0 56 36357.0 57 32153.5 58 29455.0 59 26649.0 60 22966.5 61 19160.0 62 15064.0 63 10649.5 64 6984.5 65 5247.5 66 3858.5 67 2728.5 68 1970.0 69 1404.5 70 1058.0 71 789.5 72 657.5 73 646.5 74 536.5 75 404.0 76 286.0 77 178.5 78 123.5 79 72.5 80 38.5 81 22.0 82 10.5 83 9.5 84 7.0 85 3.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04933449870439066 2 0.0 3 0.0 4 1.028873799883017E-4 5 0.010494512758806774 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004732819479461878 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011832048698654696 24-25 5.658805899356594E-4 26-27 0.0018519728397894308 28-29 0.0023406878947338635 30-31 6.68767969923961E-4 32-33 0.0 34-35 0.0 36-37 2.5721844997075426E-4 38-39 2.5721844997075426E-4 40-41 5.144368999415085E-5 42-43 0.010674565673786302 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.5433106998245257E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.629932099473577E-4 84-85 2.5721844997075424E-5 86-87 0.0 88-89 0.0 90-91 0.00810238117407876 92-93 0.00241785342972509 94-95 0.0 96-97 0.0 98-99 0.001697641769806978 100-101 0.011626273938678093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.48878812258768 #Duplication Level Percentage of deduplicated Percentage of total 1 69.69557964321584 32.40063035114396 2 15.271612225581876 14.19917490090791 3 6.084295485599342 8.485545711157336 4 3.0408630662398792 5.654641551849123 5 1.6835818104741318 3.913383903708724 6 1.026372129114045 2.8628877867187223 7 0.6775240041261219 2.2048088913050554 8 0.45646894997935816 1.6976550640104366 9 0.3406135054770991 1.4251238179035046 >10 1.5496157845345768 12.774498975834339 >50 0.10940090535903348 3.5118266747686726 >100 0.05896535716029037 4.965025172895097 >500 0.0021094680354680475 0.7076796445809661 >1k 0.0024425419358051078 2.417290385406241 >5k 2.220492668913734E-4 0.6976149132927094 >10k+ 3.3307390033706014E-4 2.082212254517201 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14883 0.7656364381829471 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13483 0.693615272191136 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11976 0.6160896313699507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8009 0.41201251316315424 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5507 0.28330040079778873 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4536 0.23334857781346827 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3718 0.19126763939825286 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3676 0.18910700441849854 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3565 0.1833967548291478 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2982 0.15340508356255783 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2835 0.14584286113341768 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2832 0.1456885300634352 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2791 0.14357933877367504 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2660 0.13684021538444127 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1949 0.1002637517986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.1443689994150854E-5 0.0 8 0.0 0.0 0.0 5.1443689994150854E-5 0.0 9 0.0 0.0 0.0 1.0288737998830171E-4 0.0 10-11 0.0 0.0 0.0 1.0288737998830171E-4 0.0 12-13 0.0 0.0 0.0 1.0288737998830171E-4 0.0 14-15 0.0 0.0 0.0 1.2860922498537713E-4 0.0 16-17 0.0 0.0 0.0 1.5433106998245257E-4 0.0 18-19 0.0 0.0 0.0 1.80052914979528E-4 0.0 20-21 0.0 0.0 0.0 2.5721844997075426E-4 0.0 22-23 0.0 0.0 0.0 4.6299320994735765E-4 0.0 24-25 0.0 0.0 0.0 7.716553499122628E-4 0.0 26-27 0.0 0.0 0.0 0.0010545956448800924 0.0 28-29 0.0 0.0 0.0 0.002366409739730939 0.0 30-31 0.0 0.0 0.0 0.0043727136495028225 0.0 32-33 0.0 0.0 0.0 0.007305003979169421 0.0 34-35 0.0 0.0 0.0 0.010957505968754132 0.0 36-37 0.0 0.0 0.0 0.017053583233061005 0.0 38-39 0.0 0.0 0.0 0.02744520861187948 0.0 40-41 0.0 2.5721844997075427E-5 0.0 0.03829982720064531 0.0 42-43 0.0 5.1443689994150854E-5 0.0 0.048640008889469635 0.0 44-45 0.0 5.1443689994150854E-5 0.0 0.056485171613577634 0.0 46-47 0.0 5.1443689994150854E-5 0.0 0.06800855817226742 0.0 48-49 0.0 5.1443689994150854E-5 0.0 0.0787088456910508 0.0 50-51 0.0 5.1443689994150854E-5 0.0 0.08948629874482542 0.0 52-53 0.0 5.1443689994150854E-5 0.0 0.11693150735670489 0.0 54-55 0.0 5.1443689994150854E-5 0.0 0.1621247890165664 0.0 56-57 0.0 7.716553499122628E-5 0.0 0.2231627271946264 2.5721844997075427E-5 58-59 0.0 1.0288737998830171E-4 0.0 0.3047009758353555 5.1443689994150854E-5 60-61 0.0 1.0288737998830171E-4 0.0 0.4130156651180401 5.1443689994150854E-5 62-63 0.0 1.0288737998830171E-4 0.0 0.4764457348808281 5.1443689994150854E-5 64-65 0.0 1.0288737998830171E-4 0.0 0.5582669238165251 5.1443689994150854E-5 66-67 0.0 1.0288737998830171E-4 0.0 0.7204174346780885 5.1443689994150854E-5 68-69 0.0 1.0288737998830171E-4 0.0 0.917343879975698 5.1443689994150854E-5 70-71 0.0 1.0288737998830171E-4 0.0 1.1215496074074798 5.1443689994150854E-5 72-73 0.0 1.0288737998830171E-4 0.0 1.375964376273553 5.1443689994150854E-5 74-75 0.0 1.0288737998830171E-4 0.0 1.6412594855733889 5.1443689994150854E-5 76-77 0.0 1.0288737998830171E-4 0.0 1.8842023115707662 5.1443689994150854E-5 78-79 0.0 1.0288737998830171E-4 0.0 2.124547231223439 5.1443689994150854E-5 80-81 0.0 1.0288737998830171E-4 0.0 2.384363587538898 5.1443689994150854E-5 82-83 0.0 1.0288737998830171E-4 0.0 2.7205995453406677 5.1443689994150854E-5 84-85 0.0 1.0288737998830171E-4 0.0 3.2231529528935274 5.1443689994150854E-5 86-87 0.0 1.0288737998830171E-4 0.0 3.829005289954642 5.1443689994150854E-5 88-89 0.0 1.0288737998830171E-4 0.0 4.516704537796451 5.1443689994150854E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 70 0.008368845 27.158882 1 GTACATG 12615 0.0 24.903715 1 TACATGG 12530 0.0 24.527096 2 TTTACGC 120 1.7114278E-4 23.749962 3 GAGTACT 12660 0.0 23.73059 12-13 ACATGGG 12500 0.0 23.673962 3 GTACTTT 13325 0.0 22.599176 14-15 AGTACTT 13045 0.0 21.974295 12-13 AGAGTAC 20305 0.0 20.503624 10-11 CATGGGA 7735 0.0 19.95795 4 ACTTTTT 15125 0.0 19.9259 16-17 CATGGGG 4210 0.0 19.857449 4 TAACGCG 120 1.4161378E-7 19.790617 76-77 TACTTTT 14925 0.0 19.1422 14-15 GTGTAGC 1440 0.0 18.813185 1 GTATCAA 30430 0.0 18.461435 1 ATGGGAT 3210 0.0 17.904957 5 CATGGGT 1965 0.0 17.646282 4 CGTATAG 190 1.8581092E-4 17.510332 1 GTATAGG 655 0.0 17.414856 1 >>END_MODULE