FastQCFastQC Report
Thu 26 May 2016
SRR937425_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937425_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159701
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150210.6955129436898905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109030.5048384012416534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104270.4827983132850334No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT80150.37111618691661485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72760.33689848733690453No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT60810.2815667539163986No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42140.195119602204194No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36840.1705791681348483No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34520.15983694039128563No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30870.14293645277749095No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30160.13964896066631446No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30150.13960265796052324No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28880.13372221432503853No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26230.12145199729036565No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23270.10774639637616504No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG22150.10256049332754857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG131250.025.4642371
TACATGG130050.025.2157692
GAGTACT118900.024.24925212-13
ACATGGG129250.024.0837463
ATGGGCG3000.023.763675
ACCGATC1000.001653043723.7526428
AGTACTT123650.023.1640612-13
GTACTTT128550.022.33575214-15
AGAGTAC197550.021.56910310-11
CATGGGG41650.021.567534
GTATCAA271200.021.5620521
CATGGGA78700.021.0163924
GTCGGGA6500.019.7416692
TACTTTT144450.019.69634414-15
CGGGAGT7050.019.5507094
ACTTTTT150500.019.41042916-17
ATGGGAT35300.018.984015
TCAACGC313350.018.6261144
CAACGCA314200.018.5450425
ATCAACG314750.018.5415443
Produced by FastQC (version 0.11.5)