##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937425_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2159701 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75468270839343 33.0 31.0 34.0 30.0 34.0 2 31.840211677449794 33.0 31.0 34.0 30.0 34.0 3 31.874569674228052 33.0 31.0 34.0 30.0 34.0 4 35.51627655865326 37.0 35.0 37.0 33.0 37.0 5 35.516261278760346 37.0 35.0 37.0 33.0 37.0 6 35.60053312935448 37.0 35.0 37.0 33.0 37.0 7 35.54849212923455 37.0 35.0 37.0 33.0 37.0 8 35.5404308281563 37.0 35.0 37.0 33.0 37.0 9 37.15548031880339 39.0 37.0 39.0 34.0 39.0 10-11 37.07031899323101 39.0 37.0 39.0 33.0 39.0 12-13 36.971108500667455 39.0 37.0 39.0 33.0 39.0 14-15 38.08979298523268 40.0 38.0 41.0 33.0 41.0 16-17 38.055476197862575 40.0 38.0 41.0 33.0 41.0 18-19 38.07124018556273 40.0 38.0 41.0 33.0 41.0 20-21 38.02638142965161 40.0 38.0 41.0 33.0 41.0 22-23 37.8876927871034 40.0 38.0 41.0 33.0 41.0 24-25 37.73754445638539 40.0 37.0 41.0 32.5 41.0 26-27 37.57269895230868 40.0 37.0 41.0 32.0 41.0 28-29 37.396512063475456 39.5 37.0 41.0 32.0 41.0 30-31 37.359878288707556 39.0 37.0 41.0 32.0 41.0 32-33 37.15600076121648 39.0 36.0 41.0 32.0 41.0 34-35 36.933723464498094 39.0 36.0 41.0 31.0 41.0 36-37 36.897920360272096 39.0 36.0 41.0 31.0 41.0 38-39 36.8515738984239 39.0 36.0 41.0 31.0 41.0 40-41 36.687756545929275 39.0 35.5 41.0 30.5 41.0 42-43 36.39838269278942 39.0 35.0 41.0 30.0 41.0 44-45 36.086004729358365 39.0 35.0 41.0 29.5 41.0 46-47 35.652429433518805 39.0 35.0 41.0 27.5 41.0 48-49 35.261394054084334 39.0 35.0 41.0 26.0 41.0 50-51 34.30494336947568 38.0 34.0 39.5 23.5 40.5 52-53 34.43065359510413 38.0 34.5 39.5 24.0 40.5 54-55 34.795966895417465 39.0 35.0 40.0 24.0 41.0 56-57 34.58212294201836 38.5 34.5 40.0 23.0 41.0 58-59 34.36154032433193 38.0 34.0 40.0 22.5 41.0 60-61 34.078473594261425 38.0 34.0 40.0 22.0 41.0 62-63 33.783920551965295 38.0 34.0 40.0 20.5 41.0 64-65 33.4595828311419 37.0 33.0 40.0 19.5 41.0 66-67 33.067751045167824 37.0 33.0 39.5 18.0 41.0 68-69 32.58273923103244 36.0 32.0 39.0 14.0 41.0 70-71 32.15604197062464 35.5 32.0 39.0 10.0 40.0 72-73 31.750685858829534 35.0 31.5 38.5 7.5 40.0 74-75 31.31452247324977 35.0 31.0 37.5 7.0 39.5 76-77 30.888425758936073 35.0 31.0 37.0 2.0 39.0 78-79 30.41825511957442 35.0 30.5 36.5 2.0 39.0 80-81 29.956262464109614 34.0 30.0 36.0 2.0 38.0 82-83 29.518221272296486 34.0 29.5 35.5 2.0 37.0 84-85 29.05029446205748 34.0 29.0 35.0 2.0 37.0 86-87 28.66186060014789 34.0 29.0 35.0 2.0 36.0 88-89 28.27314730140885 34.0 28.5 35.0 2.0 36.0 90-91 27.84355751097027 34.0 27.0 35.0 2.0 35.5 92-93 27.42640115460427 34.0 26.5 35.0 2.0 35.0 94-95 26.9242154353774 33.0 25.5 35.0 2.0 35.0 96-97 26.349534495747328 33.0 25.0 35.0 2.0 35.0 98-99 25.698490902212853 33.0 23.5 35.0 2.0 35.0 100-101 23.906163630984103 30.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.021362979361402523 1101 2 -0.03251025939821517 1101 3 0.09159882270398967 1101 4 0.002186340463516956 1101 5 0.06804257296390404 1101 6 -0.17860673956263184 1101 7 0.04328137784971631 1101 8 -0.040795678107393485 1101 9 -0.022252185837054128 1101 10-11 0.018941874708254147 1101 12-13 0.10498279103357788 1101 14-15 0.09298608524508722 1101 16-17 -0.03966084042025386 1101 18-19 0.12838157442573106 1101 20-21 0.06092682297189356 1101 22-23 0.020687069714263373 1101 24-25 0.09444260938160909 1101 26-27 0.1123306325655804 1101 28-29 0.07945829835140472 1101 30-31 -0.0399067147801091 1101 32-33 -0.006463558892853882 1101 34-35 -0.11406309752899801 1101 36-37 0.05892221293218114 1101 38-39 0.08236291492851677 1101 40-41 0.1861252358847807 1101 42-43 0.2693250107551748 1101 44-45 0.19175956502229496 1101 46-47 0.1779750764509629 1101 48-49 0.2654082924693242 1101 50-51 0.313326797410447 1101 52-53 0.19241328114623002 1101 54-55 0.32080690081112095 1101 56-57 0.3051738975340683 1101 58-59 0.26331982368485285 1101 60-61 0.18406752373446977 1101 62-63 0.31885140985811944 1101 64-65 0.2824645734518896 1101 66-67 0.4118735110519651 1101 68-69 0.08330150733844732 1101 70-71 0.2136245419631564 1101 72-73 0.17347031886145814 1101 74-75 0.19701677584190236 1101 76-77 0.28153689948652527 1101 78-79 0.43146518387633037 1101 80-81 0.49040808180463813 1101 82-83 0.39186094364105983 1101 84-85 0.25131796739211865 1101 86-87 0.33726430423570264 1101 88-89 0.31393240526259447 1101 90-91 0.37223789553980424 1101 92-93 0.3061913935319751 1101 94-95 0.4019667361817163 1101 96-97 0.4461606878176205 1101 98-99 0.588312041684695 1101 100-101 0.8354655034748006 1102 1 0.10779450346644737 1102 2 0.038734295648357175 1102 3 0.04789562520779711 1102 4 -0.0931776514938818 1102 5 -0.11739975671313374 1102 6 -0.020325925878431406 1102 7 -0.11644785368563504 1102 8 -0.0026669482975663072 1102 9 0.007389511462349674 1102 10-11 -0.1134109877241869 1102 12-13 0.027180698190257147 1102 14-15 0.08671267562662166 1102 16-17 0.13370135640928993 1102 18-19 0.1005340009941591 1102 20-21 0.26181581954762834 1102 22-23 0.24553486536151325 1102 24-25 0.2599516238416939 1102 26-27 0.26752753439711086 1102 28-29 0.12329418939611259 1102 30-31 0.23870505970578648 1102 32-33 0.11614946321358843 1102 34-35 0.18333192564300305 1102 36-37 0.26458784259235557 1102 38-39 0.460497026105962 1102 40-41 0.31426051837981106 1102 42-43 0.25896554343025 1102 44-45 0.3010578855536892 1102 46-47 0.4023965124996991 1102 48-49 0.3358938337251445 1102 50-51 0.34290691687145625 1102 52-53 0.3337037986945717 1102 54-55 0.47162960081192296 1102 56-57 0.3212107411611669 1102 58-59 0.22590894421945507 1102 60-61 0.35490820848003324 1102 62-63 0.411352868985162 1102 64-65 0.513956417065593 1102 66-67 0.4198093549762447 1102 68-69 0.45773559361703775 1102 70-71 0.3748346535796827 1102 72-73 0.21216462773054445 1102 74-75 0.21574865388565811 1102 76-77 0.17995254186385523 1102 78-79 0.3437716487461735 1102 80-81 0.4280389347112461 1102 82-83 0.2685334178118346 1102 84-85 0.23421438811332962 1102 86-87 0.34105134129752557 1102 88-89 0.44552223795828283 1102 90-91 0.5202705170690258 1102 92-93 0.34978632781771424 1102 94-95 0.19374763420078267 1102 96-97 0.37109512749945495 1102 98-99 0.5384328212601304 1102 100-101 0.5022967681497335 1103 1 -0.03629983308774598 1103 2 -0.11413148502862924 1103 3 -0.022363335765405168 1103 4 -0.06068138026305547 1103 5 -0.14362302458101084 1103 6 -0.03690053556344708 1103 7 0.11893838342125207 1103 8 0.0010412747745931483 1103 9 0.15032490859933745 1103 10-11 -0.04290547353361518 1103 12-13 0.05728979102416076 1103 14-15 0.15918312841743187 1103 16-17 0.11928234618116562 1103 18-19 0.13917146712356043 1103 20-21 0.14896496439451568 1103 22-23 0.09934534881823964 1103 24-25 0.0878632240660977 1103 26-27 0.072177378333798 1103 28-29 -0.06448507652302027 1103 30-31 -0.10077512740999595 1103 32-33 -0.03142830813337838 1103 34-35 0.11613520438291403 1103 36-37 0.15200556677383048 1103 38-39 0.13233460673635022 1103 40-41 0.18287055024020304 1103 42-43 0.12199347811871775 1103 44-45 0.11848017863093929 1103 46-47 0.17515854581154144 1103 48-49 0.2045925794727097 1103 50-51 0.21355823873449253 1103 52-53 0.17184605154325538 1103 54-55 0.15339025196315248 1103 56-57 0.12088294378333586 1103 58-59 0.2514487059752213 1103 60-61 0.25138806929586366 1103 62-63 0.2655448482746934 1103 64-65 0.13664746185575183 1103 66-67 0.3224490414775545 1103 68-69 0.2513950144746744 1103 70-71 0.3460080379509236 1103 72-73 0.40571012219685443 1103 74-75 0.5375326405327812 1103 76-77 0.44256932816803385 1103 78-79 0.6936666198614851 1103 80-81 0.6207946231083739 1103 82-83 0.5425905519928484 1103 84-85 0.6015264461327341 1103 86-87 0.7957440534560511 1103 88-89 0.8974123225838433 1103 90-91 0.9043141535061068 1103 92-93 1.0113276998306482 1103 94-95 1.1070244553079682 1103 96-97 1.1088938773910648 1103 98-99 1.024516319919929 1103 100-101 1.39163633635426 1104 1 0.1403235553435067 1104 2 0.24180890738495364 1104 3 0.22825547026445392 1104 4 0.06837395405221969 1104 5 0.09558905695663356 1104 6 0.14622549865909207 1104 7 0.1277207739073134 1104 8 0.25141877736583496 1104 9 0.23926186421187623 1104 10-11 0.14489042776290972 1104 12-13 0.2575785550486813 1104 14-15 0.40670235813757927 1104 16-17 0.2999054254821658 1104 18-19 0.3881222426768147 1104 20-21 0.30634972245462677 1104 22-23 0.36577234861822205 1104 24-25 0.33031671814715935 1104 26-27 0.34832252407962727 1104 28-29 0.3134332039854044 1104 30-31 0.14547948681363465 1104 32-33 0.3290323868641636 1104 34-35 0.3945741875262172 1104 36-37 0.29929967693330894 1104 38-39 0.28949279050519294 1104 40-41 0.20842462075826518 1104 42-43 0.29262083069086486 1104 44-45 0.36766198358793645 1104 46-47 0.273098101755032 1104 48-49 0.38097239144877193 1104 50-51 0.23263757610470037 1104 52-53 0.23290573186621089 1104 54-55 0.34141818279071146 1104 56-57 0.48088094352876 1104 58-59 0.23231954408188926 1104 60-61 0.3227659719380185 1104 62-63 0.3560780979610101 1104 64-65 0.4270053255838633 1104 66-67 0.5364722807989253 1104 68-69 0.635907406960996 1104 70-71 0.6664436388455854 1104 72-73 0.7752975242298383 1104 74-75 0.8398522993984585 1104 76-77 0.787284837414667 1104 78-79 0.731640634736106 1104 80-81 0.8678074247376735 1104 82-83 0.8827469355325768 1104 84-85 1.0163606068440956 1104 86-87 0.9190698403743873 1104 88-89 0.8758525786164313 1104 90-91 0.9010345542843865 1104 92-93 0.9408548214643098 1104 94-95 1.02379813559455 1104 96-97 1.2081636935494835 1104 98-99 1.238492735100042 1104 100-101 1.4702379765630127 1105 1 0.20888233151098134 1105 2 0.2803797608471079 1105 3 0.2743947617298339 1105 4 0.23523385743386171 1105 5 0.24954638384874528 1105 6 0.21029554696826835 1105 7 0.2228053149701168 1105 8 0.22745339894392913 1105 9 0.3549301411844965 1105 10-11 0.39942746479994895 1105 12-13 0.38704111543514585 1105 14-15 0.5686991375256625 1105 16-17 0.4985306267704104 1105 18-19 0.5238710349473479 1105 20-21 0.5751580960456053 1105 22-23 0.5856455370240141 1105 24-25 0.5996766215288005 1105 26-27 0.46784748382197705 1105 28-29 0.6515048626005395 1105 30-31 0.6283639473611302 1105 32-33 0.5733782000686816 1105 34-35 0.5779236239191334 1105 36-37 0.5668781793487625 1105 38-39 0.4979972188465851 1105 40-41 0.6256609331576186 1105 42-43 0.5848893492093836 1105 44-45 0.6503854135396239 1105 46-47 0.5717615478097855 1105 48-49 0.7172787521573127 1105 50-51 0.646148043093433 1105 52-53 0.5265087914475259 1105 54-55 0.6489322729678477 1105 56-57 0.7607279644627383 1105 58-59 0.9455582719401789 1105 60-61 0.9345997078478447 1105 62-63 0.9480225424054609 1105 64-65 0.8340000920894965 1105 66-67 0.9754356463383758 1105 68-69 0.985547101002858 1105 70-71 0.8117153779760145 1105 72-73 0.8917387480623695 1105 74-75 0.8752307212986139 1105 76-77 0.9369748535177251 1105 78-79 1.1040379777312772 1105 80-81 1.0839809352046608 1105 82-83 1.0969840530849098 1105 84-85 0.9142370158618114 1105 86-87 0.8946374732246234 1105 88-89 0.992893641418366 1105 90-91 1.0825220744132125 1105 92-93 1.2569156105142305 1105 94-95 1.285732515626755 1105 96-97 1.1994237579617213 1105 98-99 1.3046199492707373 1105 100-101 1.7902782342119679 1106 1 0.19358779705925855 1106 2 0.16804828511077474 1106 3 0.19676587896909936 1106 4 0.18133290373973665 1106 5 0.12042472082067235 1106 6 0.15703661326724472 1106 7 0.29026337540904734 1106 8 0.22255901386598964 1106 9 0.22963235115040703 1106 10-11 0.10560397481565076 1106 12-13 0.09212915978766745 1106 14-15 0.19948258384685147 1106 16-17 0.20382453883138396 1106 18-19 0.23759618719369513 1106 20-21 0.12583985329406744 1106 22-23 0.17193259321153676 1106 24-25 0.12244319627314582 1106 26-27 0.2311695183694482 1106 28-29 0.3506023960181395 1106 30-31 0.41628070978022436 1106 32-33 0.500926411413424 1106 34-35 0.4057713009653696 1106 36-37 0.2551530558281385 1106 38-39 0.2676884651601128 1106 40-41 0.5236570743349915 1106 42-43 0.5623829816732808 1106 44-45 0.5900132717494415 1106 46-47 0.575304387443623 1106 48-49 0.7589911406057368 1106 50-51 0.6077551517532811 1106 52-53 0.7151846311962728 1106 54-55 0.7809267480530622 1106 56-57 0.6882596585145961 1106 58-59 0.5416082451294955 1106 60-61 0.6781998553065804 1106 62-63 0.8587099490468475 1106 64-65 0.7854289576147977 1106 66-67 0.6730281910547617 1106 68-69 0.6009323608749355 1106 70-71 0.7319777874899813 1106 72-73 0.8757251129173973 1106 74-75 0.6666943928295055 1106 76-77 0.6898957811671949 1106 78-79 0.7080789693433651 1106 80-81 0.6578876819203536 1106 82-83 0.4551294722890127 1106 84-85 0.4869071200404491 1106 86-87 0.38783091226863675 1106 88-89 0.5609721775553282 1106 90-91 0.6890784821653284 1106 92-93 0.633811747861504 1106 94-95 0.6669686315254886 1106 96-97 0.8565443868636358 1106 98-99 0.9759669943509799 1106 100-101 1.289727657782386 1107 1 0.13917900360584667 1107 2 0.12962764176071673 1107 3 0.16600558055316483 1107 4 0.20797593434730288 1107 5 0.2932543674806283 1107 6 0.1570558501124495 1107 7 0.21811879361223419 1107 8 0.1990894797823799 1107 9 0.23872904719309673 1107 10-11 0.21744961603798885 1107 12-13 0.19740454397079077 1107 14-15 0.3138502920623907 1107 16-17 0.3152460950571836 1107 18-19 0.33106131429094887 1107 20-21 0.3963614865838352 1107 22-23 0.4365732897485586 1107 24-25 0.41473831512770687 1107 26-27 0.3473862954633802 1107 28-29 0.40524144867929834 1107 30-31 0.29049884860962294 1107 32-33 0.3745225589145562 1107 34-35 0.44324263515133566 1107 36-37 0.41039643689605043 1107 38-39 0.5554993588106711 1107 40-41 0.4880155721822135 1107 42-43 0.5577955770268233 1107 44-45 0.42191005966263617 1107 46-47 0.50100702778807 1107 48-49 0.4900069975955361 1107 50-51 0.5171280422583209 1107 52-53 0.5355754539386481 1107 54-55 0.7091432943048517 1107 56-57 0.669739822995453 1107 58-59 0.685767414284328 1107 60-61 0.6880301746731803 1107 62-63 0.7988571284007051 1107 64-65 0.9365244668024317 1107 66-67 0.8273790990921484 1107 68-69 0.8021404347537455 1107 70-71 0.9392731570064612 1107 72-73 1.042868231548102 1107 74-75 0.975874798295564 1107 76-77 0.9380088215330922 1107 78-79 1.0077356790966796 1107 80-81 1.1574341587213013 1107 82-83 1.1632214220773527 1107 84-85 1.2137001980406055 1107 86-87 1.12412670033223 1107 88-89 1.1819500738372533 1107 90-91 1.2736503574312898 1107 92-93 1.3206085100089915 1107 94-95 1.2942900232471146 1107 96-97 1.3798702275429129 1107 98-99 1.639934576186267 1107 100-101 2.066714619864811 1108 1 0.06618913767803392 1108 2 -0.024968477250382648 1108 3 -0.03516610399315567 1108 4 -0.028630177030933623 1108 5 0.025975118719109958 1108 6 0.10615491826969503 1108 7 0.03610539071693353 1108 8 0.1108106004146805 1108 9 0.10913330045796243 1108 10-11 0.048665459718598925 1108 12-13 0.1490188766433107 1108 14-15 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2316 100-101 -2.06987272783838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7111.0 3 1201.0 4 1061.0 5 1226.0 6 1405.0 7 1810.0 8 2229.0 9 2854.0 10 3871.0 11 5394.0 12 8084.0 13 10921.0 14 13036.0 15 16619.0 16 22327.0 17 30480.0 18 24409.0 19 14509.0 20 13288.0 21 13599.0 22 14251.0 23 15843.0 24 17770.0 25 20250.0 26 23789.0 27 27558.0 28 33132.0 29 39748.0 30 48804.0 31 60913.0 32 77961.0 33 103140.0 34 139153.0 35 196706.0 36 288525.0 37 417790.0 38 381381.0 39 57533.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72253780094344 19.615760266061425 15.468512488696987 21.19318944429815 2 14.736129063409994 21.253602840672727 40.96267345634991 23.04759463956736 3 18.87550219449651 26.520048082525882 31.029433135991923 23.57501658698568 4 11.539784796462026 17.43116156515885 39.88768557532704 31.141368063052084 5 12.207346292244477 38.771025037151134 34.78683533585046 14.23479333475392 6 28.868973606167348 37.9916598309696 18.41044989403123 14.72891666883182 7 26.025391304670602 34.63608920827792 21.86443556267417 17.474083924377304 8 25.642644835781176 33.480633657412604 20.854765654216664 20.021955852589556 9 26.44869556502781 16.698363708827678 21.847992461377785 35.00494826476673 10-11 23.855758565353092 27.156061722835602 28.11847710867524 20.86970260313607 12-13 25.8232831191899 25.248516741282977 28.310898172807153 20.61730196671997 14-15 22.613151236059934 26.066607963523637 28.514417683687793 22.805823116728632 16-17 20.351930691243215 29.35887683214587 28.699572272135793 21.589620204475125 18-19 20.093254486953025 27.7851375052511 31.997131059413377 20.124476948382497 20-21 21.897829183310833 26.17372100899597 31.657294899810246 20.271154907882956 22-23 21.00600507602556 26.466223738086175 31.77556738770726 20.75220379818101 24-25 20.99936537626507 26.551024119413174 31.91396580454331 20.535644699778437 26-27 20.71960176882344 26.49975804180425 32.370387657394616 20.410252531977687 28-29 20.21236591130898 27.378764290401985 31.971782596360242 20.437087201928794 30-31 20.666853811372057 26.628077737745308 31.798932347189 20.906136103693633 32-33 20.43887049879683 26.776028167001215 31.856101628381555 20.928999705820395 34-35 21.244633201910382 26.760175226479575 31.561017954125997 20.434173617484046 36-37 20.868810403808222 26.388955580562033 31.992610330644556 20.74962368498519 38-39 20.49211679296019 27.13531309807149 31.68160161326649 20.69096849570183 40-41 20.821367543373164 27.15992511682809 31.405794469305764 20.612912870492984 42-43 20.504633301084663 27.162085399517405 30.81862524177804 21.51465605761989 44-45 20.885368976429426 26.942780646812142 30.684266435174607 21.487583941583825 46-47 21.39618088876301 27.43336063556439 30.23036634398074 20.94009213169186 48-49 21.170188481753126 27.544949075036374 29.850445036671914 21.434417406538586 50-51 20.7889155479378 27.768526247460212 30.13997779323394 21.302580411368048 52-53 21.212227373313244 28.25503607954901 29.053769687610636 21.4789668595271 54-55 21.0003614679776 27.64455122984955 29.44087075269326 21.914216549479583 56-57 21.50811523054482 27.76035779982702 28.928013033169037 21.803513936459126 58-59 21.812685850996377 27.66286124841776 29.106695343746942 21.41775755683892 60-61 21.50452966198006 27.784454886607694 29.80712407052635 20.903891380885895 62-63 21.15353661019984 27.854919265843115 29.701351576390717 21.290192547566335 64-65 20.94764827399544 28.301400179002854 29.347817767761537 21.403133779240168 66-67 21.19979745830116 27.897126816571955 28.840713201000835 22.06236252412605 68-69 21.234698641603273 27.782226987631354 29.190535266617452 21.792539104147924 70-71 21.562044811430162 27.685809713782067 29.079069443800158 21.673076030987616 72-73 21.29428073554874 27.396225942400942 29.685606545634048 21.623886776416267 74-75 20.81901937466933 28.465671201440106 29.063952343476558 21.651357080414005 76-77 20.80970363909663 27.771749215951903 29.184520385250618 22.234026759700857 78-79 20.953591131257784 27.85364868102929 29.21440734521582 21.97835284249711 80-81 21.21639721819238 27.95656353753898 28.861984629782317 21.96505461448632 82-83 21.069453890555426 27.749684296279938 29.337991799590075 21.842870013574565 84-85 21.02244289546425 27.759486266755175 29.0491073727159 22.168963465064685 86-87 20.816310247806648 28.009273701527547 28.967514444351412 22.206901606314393 88-89 20.934310070643512 27.617934025232742 28.71131261098936 22.736443293134386 90-91 20.869665659868655 27.13854892124477 29.175354957765048 22.81643046112153 92-93 21.304404907651005 27.33655969897563 29.311463819596923 22.04757157377644 94-95 20.822405419178434 27.144880772368392 29.498629966039676 22.534083842413505 96-97 20.18225802336558 27.332232394055854 29.878069428756316 22.607440153822246 98-99 20.506727197995154 27.560125404879805 28.962603069803087 22.970544327321953 100-101 20.411559741286222 26.968949337073774 29.767321800309475 22.85216912133053 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3153.0 1 1845.0 2 571.0 3 570.5 4 512.5 5 574.0 6 869.0 7 1740.5 8 3001.5 9 3740.0 10 3576.0 11 3267.0 12 3403.0 13 3682.0 14 3876.5 15 4087.0 16 4289.0 17 4517.5 18 4861.0 19 5238.0 20 5836.5 21 6621.0 22 7547.0 23 8918.5 24 10577.5 25 12755.0 26 16079.0 27 19785.5 28 22601.0 29 25804.0 30 30455.5 31 34966.0 32 39808.5 33 45970.5 34 53580.5 35 59043.5 36 64766.5 37 71092.5 38 72659.0 39 72553.0 40 72469.5 41 72806.5 42 73242.0 43 74879.5 44 77845.0 45 79224.5 46 78885.5 47 86248.0 48 103095.0 49 99044.0 50 78401.0 51 66267.0 52 59235.5 53 55117.0 54 50947.0 55 45886.5 56 40924.0 57 36409.5 58 33294.0 59 30017.0 60 25787.0 61 21535.5 62 17085.0 63 12155.0 64 8193.0 65 6116.5 66 4515.0 67 3252.0 68 2331.0 69 1656.0 70 1217.5 71 968.5 72 810.0 73 703.0 74 573.0 75 432.0 76 315.0 77 200.0 78 133.0 79 90.5 80 53.0 81 28.5 82 17.5 83 16.5 84 10.0 85 3.0 86 4.5 87 5.5 88 6.0 89 6.0 90 6.0 91 4.0 92 2.0 93 2.5 94 3.0 95 3.5 96 2.0 97 0.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3016158255239962 2 0.2706393153496711 3 0.27337117499135294 4 0.27438983451875976 5 0.2621659201898781 6 0.20137046748600848 7 0.207204608415702 8 0.20683418676937224 9 0.2037319054813606 10-11 0.210700462702939 12-13 0.21134870058401603 14-15 0.195536326556315 16-17 0.20722775976859759 18-19 0.1945408183818038 20-21 0.19868491055011783 22-23 0.19792091590456273 24-25 0.18951697480345658 26-27 0.2023428243076241 28-29 0.20208815942577238 30-31 0.20863999229522975 32-33 0.21090882487899945 34-35 0.1999813863122719 36-37 0.19722637531769446 38-39 0.18866037474631905 40-41 0.18875298015790148 42-43 0.18782692604207712 44-45 0.20407917577479473 46-47 0.19444821297022133 48-49 0.2024817324249977 50-51 0.2084547814720649 52-53 0.21308505205118672 54-55 0.2129924466396043 56-57 0.18384489334403234 58-59 0.17317211965915652 60-61 0.18108988234945486 62-63 0.18159921211315827 64-65 0.1791683200591193 66-67 0.18963273156793464 68-69 0.18000176876336121 70-71 0.1852571258706645 72-73 0.19674019690688666 74-75 0.18639154216254936 76-77 0.19544372114473252 78-79 0.1933369480312321 80-81 0.1809741255849768 82-83 0.19442506161732573 84-85 0.17937668223517977 86-87 0.18185387699500996 88-89 0.1893317639802917 90-91 0.1862294826922801 92-93 0.19113756950614924 94-95 0.18972533697951707 96-97 0.18363653116797188 98-99 0.19090605597719315 100-101 0.17618179553558572 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2159701.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.91440235870082 #Duplication Level Percentage of deduplicated Percentage of total 1 70.12443736611823 32.898460697715855 2 14.94522319273571 14.022924284091806 3 6.015220780876838 8.466014639714235 4 2.9267559251710544 5.49228020316746 5 1.719831346133402 4.0342429880804245 6 1.0517068069571793 2.960411778298416 7 0.6810480628355918 2.23656739918379 8 0.4760997831775574 1.7868749432705717 9 0.34125481079968806 1.4408788950629 >10 1.5484402315441397 12.803141231685913 >50 0.10750170274937082 3.5068660948413335 >100 0.057637994747718746 4.793952744708939 >500 0.002173957456664183 0.7086908309302662 >1k 0.002075141208633979 2.1727096993006234 >5k 2.964487440905684E-4 0.9907856635469077 >10k+ 2.964487440905684E-4 1.6851979064006009 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15021 0.6955129436898905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10903 0.5048384012416534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10427 0.4827983132850334 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8015 0.37111618691661485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7276 0.33689848733690453 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6081 0.2815667539163986 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4214 0.195119602204194 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3684 0.1705791681348483 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3452 0.15983694039128563 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3087 0.14293645277749095 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3016 0.13964896066631446 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3015 0.13960265796052324 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2888 0.13372221432503853 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2623 0.12145199729036565 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2327 0.10774639637616504 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2215 0.10256049332754857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.630270579121832E-5 0.0 8 0.0 0.0 0.0 4.630270579121832E-5 0.0 9 0.0 0.0 0.0 9.260541158243664E-5 0.0 10-11 0.0 0.0 0.0 9.260541158243664E-5 0.0 12-13 0.0 0.0 0.0 9.260541158243664E-5 0.0 14-15 0.0 0.0 0.0 1.157567644780458E-4 0.0 16-17 0.0 0.0 0.0 1.3890811737365496E-4 0.0 18-19 0.0 0.0 0.0 1.3890811737365496E-4 0.0 20-21 0.0 0.0 0.0 1.8521082316487328E-4 0.0 22-23 0.0 0.0 0.0 3.009675876429191E-4 0.0 24-25 0.0 0.0 0.0 6.482378810770564E-4 0.0 26-27 0.0 0.0 0.0 0.0010881135860936306 0.0 28-29 0.0 0.0 0.0 0.002361437995352134 0.0 30-31 0.0 0.0 0.0 0.005718384165215463 0.0 32-33 0.0 0.0 0.0 0.009191087099556837 0.0 34-35 0.0 0.0 0.0 0.014029719854739152 0.0 36-37 0.0 0.0 0.0 0.02069730948867459 0.0 38-39 0.0 0.0 0.0 0.031995169701731864 0.0 40-41 0.0 0.0 0.0 0.042806851503981336 0.0 42-43 0.0 0.0 0.0 0.05320180895410985 0.0 44-45 0.0 0.0 0.0 0.061096420291512576 0.0 46-47 0.0 0.0 0.0 0.0730425183856469 0.0 48-49 0.0 0.0 0.0 0.08267348119022032 0.0 50-51 0.0 0.0 0.0 0.09283692511139274 0.0 52-53 0.0 0.0 0.0 0.11716899700467795 0.0 54-55 0.0 0.0 0.0 0.15779962133647202 0.0 56-57 0.0 0.0 0.0 0.22079445256542457 0.0 58-59 0.0 0.0 0.0 0.30226406340507317 0.0 60-61 0.0 0.0 0.0 0.4068155730816442 0.0 62-63 0.0 0.0 0.0 0.467425814962349 0.0 64-65 0.0 0.0 0.0 0.5510021989154981 0.0 66-67 0.0 0.0 0.0 0.711996706951564 0.0 68-69 0.0 0.0 0.0 0.9042223900438069 0.0 70-71 0.0 0.0 0.0 1.1065883657043267 0.0 72-73 0.0 0.0 0.0 1.3535901497475806 0.0 74-75 0.0 0.0 0.0 1.5964246902696253 0.0 76-77 0.0 0.0 0.0 1.8289800301060193 0.0 78-79 0.0 0.0 0.0 2.059613807652078 0.0 80-81 0.0 0.0 0.0 2.3000637588258743 0.0 82-83 0.0 0.0 0.0 2.613463622973736 0.0 84-85 0.0 0.0 0.0 3.070679691309121 0.0 86-87 0.0 0.0 0.0 3.621033652343542 0.0 88-89 0.0 0.0 0.0 4.253135040452358 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13125 0.0 25.464237 1 TACATGG 13005 0.0 25.215769 2 GAGTACT 11890 0.0 24.249252 12-13 ACATGGG 12925 0.0 24.083746 3 ATGGGCG 300 0.0 23.76367 5 ACCGATC 100 0.0016530437 23.752642 8 AGTACTT 12365 0.0 23.16406 12-13 GTACTTT 12855 0.0 22.335752 14-15 AGAGTAC 19755 0.0 21.569103 10-11 CATGGGG 4165 0.0 21.56753 4 GTATCAA 27120 0.0 21.562052 1 CATGGGA 7870 0.0 21.016392 4 GTCGGGA 650 0.0 19.741669 2 TACTTTT 14445 0.0 19.696344 14-15 CGGGAGT 705 0.0 19.550709 4 ACTTTTT 15050 0.0 19.410429 16-17 ATGGGAT 3530 0.0 18.98401 5 TCAACGC 31335 0.0 18.626114 4 CAACGCA 31420 0.0 18.545042 5 ATCAACG 31475 0.0 18.541544 3 >>END_MODULE