FastQCFastQC Report
Thu 26 May 2016
SRR937425_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937425_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159701
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160240.7419545575984824No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT139110.644116940261638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131460.608695370331356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88000.4074638109627212No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58290.2698984720570116No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52730.24415416763709422No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39280.18187702834790556No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC39140.1812287904668285No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39060.18085836882049877No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32770.15173396687782242No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30880.14298275548328215No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30530.14136216078058952No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29060.13455566302928043No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28850.13358330620766484No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23120.10705185578929677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT134450.024.32359312-13
GTACATG132900.023.3888531
ATGGGCG2653.092282E-1123.3046425
GTACTTT140500.023.1742514-15
TACATGG131900.023.0144712
ACATGGG132350.021.9670853
AGTACTT139150.021.8635412-13
AGAGTAC217100.020.6208310-11
ACTTTTT162450.020.36460316-17
TACTTTT155450.019.5552714-15
GTATCAA323450.019.2936211
CATGGGA83650.018.5138454
CATGGGG43450.018.0401084
GGTATCA260350.017.5437321
CATGGGC23100.017.4804214
CAACGCA364000.017.0184965
TCAACGC364000.016.9923934
ATCAACG366350.016.896363
AACGCAG370000.016.7269696
TATCAAC372750.016.644492