##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937425_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2159701 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11956053175879 33.0 31.0 34.0 31.0 34.0 2 32.322427039668916 34.0 31.0 34.0 31.0 34.0 3 32.393785991672 34.0 31.0 34.0 31.0 34.0 4 36.02335369571992 37.0 35.0 37.0 35.0 37.0 5 35.93091728901362 37.0 35.0 37.0 35.0 37.0 6 36.0275366821611 37.0 35.0 37.0 35.0 37.0 7 35.978685938470186 37.0 35.0 37.0 35.0 37.0 8 35.97149698036904 37.0 35.0 37.0 35.0 37.0 9 37.64774244212509 39.0 37.0 39.0 35.0 39.0 10-11 37.57601630966509 39.0 37.0 39.0 35.0 39.0 12-13 37.509825434168896 39.0 37.0 39.0 35.0 39.0 14-15 38.644908716530665 40.0 38.0 41.0 34.0 41.0 16-17 38.64980522766809 40.0 38.0 41.0 34.5 41.0 18-19 38.714318093106414 40.0 38.0 41.0 34.5 41.0 20-21 38.63226182698438 40.0 38.0 41.0 34.0 41.0 22-23 38.50072301675093 40.0 38.0 41.0 34.0 41.0 24-25 38.35454352245982 40.0 38.0 41.0 34.0 41.0 26-27 38.2847384892631 40.0 38.0 41.0 34.0 41.0 28-29 38.192400244293076 40.0 38.0 41.0 34.0 41.0 30-31 38.212280079511004 40.0 37.5 41.0 34.0 41.0 32-33 38.07351087025472 40.0 37.0 41.0 34.0 41.0 34-35 37.92316204882064 40.0 37.0 41.0 33.0 41.0 36-37 37.735594417931 40.0 37.0 41.0 33.0 41.0 38-39 37.58289920688095 40.0 37.0 41.0 33.0 41.0 40-41 37.407145016833354 40.0 37.0 41.0 33.0 41.0 42-43 37.14575512999254 40.0 36.0 41.0 32.5 41.0 44-45 36.755133928261365 40.0 36.0 41.0 31.0 41.0 46-47 36.27426759537548 39.5 36.0 41.0 30.0 41.0 48-49 35.864573383074784 39.0 35.0 41.0 28.5 41.0 50-51 35.60320085974864 39.0 35.0 41.0 28.0 41.0 52-53 35.38448331505148 39.0 35.0 40.5 27.0 41.0 54-55 35.1650547923069 39.0 35.0 40.0 26.5 41.0 56-57 34.90795022088706 38.5 35.0 40.0 26.0 41.0 58-59 34.67927365871479 38.0 34.0 40.0 26.0 41.0 60-61 34.340001231651975 38.0 34.0 40.0 24.5 41.0 62-63 34.00478052285942 37.5 34.0 40.0 23.0 41.0 64-65 33.67800635365729 37.0 33.5 40.0 22.5 41.0 66-67 33.24868048864171 36.5 33.0 39.0 21.5 41.0 68-69 32.848812173536984 36.0 33.0 39.0 20.5 40.0 70-71 32.43528247660208 35.5 32.0 38.5 19.5 40.0 72-73 31.99418553772027 35.0 32.0 38.0 18.0 40.0 74-75 31.552614922158206 35.0 31.0 37.0 16.0 39.0 76-77 30.215429821072455 34.0 29.5 36.0 12.5 38.5 78-79 30.850744616963183 34.5 31.0 36.0 11.5 39.0 80-81 30.69211548265246 34.5 31.0 36.0 7.5 37.5 82-83 30.356397251286175 34.0 31.0 35.5 6.5 37.0 84-85 29.988303936517138 34.0 30.5 35.0 2.0 37.0 86-87 29.643346000210215 34.0 30.0 35.0 2.0 36.0 88-89 29.245672896387045 34.0 30.0 35.0 2.0 36.0 90-91 28.811339856767212 34.0 29.0 35.0 2.0 35.5 92-93 28.465413499368665 34.0 29.0 35.0 2.0 35.0 94-95 28.061863656126473 34.0 29.0 35.0 2.0 35.0 96-97 27.599738806436633 33.0 27.5 35.0 2.0 35.0 98-99 27.154879541195747 33.0 27.0 35.0 2.0 35.0 100-101 25.293078764143743 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10454719190543926 1101 2 0.07857710003540319 1101 3 0.03291513671759816 1101 4 0.037830427918102316 1101 5 0.012170552182887207 1101 6 0.0640868442936906 1101 7 0.07845919141121271 1101 8 -0.019608566359735846 1101 9 0.15962005830603232 1101 10-11 -0.041482532166469355 1101 12-13 0.08484198821423661 1101 14-15 -0.048035058194415114 1101 16-17 -0.010111538518792429 1101 18-19 0.052332887210475576 1101 20-21 0.10393297959063119 1101 22-23 0.02907980591881909 1101 24-25 0.08148972755120809 1101 26-27 0.08469580883270567 1101 28-29 0.03029954674050117 1101 30-31 0.007069541537923385 1101 32-33 -0.06366604085442873 1101 34-35 -0.03925386561600419 1101 36-37 -0.09701364848471883 1101 38-39 -0.22515418997567593 1101 40-41 -0.16289479225551418 1101 42-43 -0.024030293917327583 1101 44-45 -0.02253752345786353 1101 46-47 0.08344791019705156 1101 48-49 0.012754048253619032 1101 50-51 -0.1736872137197949 1101 52-53 -0.3269526804668885 1101 54-55 -0.39055751321945564 1101 56-57 -0.34025935556763187 1101 58-59 -0.4005075608503006 1101 60-61 -0.40879296913763596 1101 62-63 -0.4520713742178728 1101 64-65 -0.44409999787719556 1101 66-67 -0.3927740163409297 1101 68-69 -0.3753048387246096 1101 70-71 -0.47599766363721585 1101 72-73 -0.5689615691308667 1101 74-75 -0.47560047739283107 1101 76-77 -0.46214918642618485 1101 78-79 -0.355259802067593 1101 80-81 -0.417161374826474 1101 82-83 -0.45429955971615854 1101 84-85 -0.48212595854317186 1101 86-87 -0.3700330833804024 1101 88-89 -0.4523468154879744 1101 90-91 -0.30072181217554217 1101 92-93 -0.5425046708691923 1101 94-95 -0.5487955636438322 1101 96-97 -0.47588115546023957 1101 98-99 -0.4568330522822386 1101 100-101 -0.09972171194100454 1102 1 0.1307224584973241 1102 2 0.13899198741489016 1102 3 0.08802060884559637 1102 4 0.08924424170136547 1102 5 0.03906902371384291 1102 6 0.10077418415784933 1102 7 -0.0019430591249189888 1102 8 0.13744069055638874 1102 9 0.08782431467331975 1102 10-11 0.06841500097176834 1102 12-13 0.03485667988329055 1102 14-15 0.03662309109703443 1102 16-17 0.0840247452854328 1102 18-19 0.1378099548202627 1102 20-21 0.044377169989310516 1102 22-23 0.07304772118771297 1102 24-25 0.1524024650609448 1102 26-27 0.05684170948892131 1102 28-29 0.05984954660663533 1102 30-31 0.06494266977244223 1102 32-33 0.020619127453862518 1102 34-35 0.06560549595567267 1102 36-37 0.054395881622831155 1102 38-39 -0.045659476299242385 1102 40-41 0.0426026696666284 1102 42-43 -0.003095334839819941 1102 44-45 -0.023801877118131642 1102 46-47 -0.08440675137918419 1102 48-49 -0.130593302962275 1102 50-51 -0.19285264659860957 1102 52-53 -0.07474474683174037 1102 54-55 -0.06966703074370884 1102 56-57 -0.2151507576950138 1102 58-59 -0.12882965176596173 1102 60-61 -0.2028522471776384 1102 62-63 -0.31507808620225575 1102 64-65 -0.2804461831301097 1102 66-67 -0.11874995814719824 1102 68-69 -0.015658971534627142 1102 70-71 -0.18666766398526846 1102 72-73 -0.13069285013731502 1102 74-75 -0.13549821132837891 1102 76-77 -0.07618632321733898 1102 78-79 -0.057829639368236485 1102 80-81 0.015395832324049508 1102 82-83 -0.16116194127922157 1102 84-85 -0.15685223835822626 1102 86-87 -0.09282170272494739 1102 88-89 -0.12585040708233564 1102 90-91 -0.19191996296628488 1102 92-93 -0.24972480628688842 1102 94-95 -0.1504850051863862 1102 96-97 -0.07150183329837745 1102 98-99 0.1005133177041877 1102 100-101 0.4636136048234256 1103 1 0.0565701854194387 1103 2 0.030680950084011727 1103 3 0.03835824397538801 1103 4 0.027039526614849763 1103 5 -0.027123202128905177 1103 6 -0.04960534412455786 1103 7 -0.043875736863491 1103 8 0.005799380913060759 1103 9 -0.05516784542190578 1103 10-11 -0.06535613844188504 1103 12-13 -0.04101406563722776 1103 14-15 -0.05578047542758213 1103 16-17 0.019861272841062316 1103 18-19 0.06858639547633771 1103 20-21 0.060698435796304295 1103 22-23 -0.028214697450593462 1103 24-25 -0.06691201948786585 1103 26-27 0.1038089656197485 1103 28-29 -0.14011677868133177 1103 30-31 -0.050043412855622194 1103 32-33 -0.028449489908425107 1103 34-35 0.08980375399450935 1103 36-37 0.05765313518018189 1103 38-39 0.03635063586505538 1103 40-41 0.030092357439016837 1103 42-43 0.04402880296392908 1103 44-45 0.013464310859198747 1103 46-47 0.032266984539340626 1103 48-49 0.08646032688960759 1103 50-51 0.08310944201358694 1103 52-53 -0.01982014231807483 1103 54-55 0.08171037641490386 1103 56-57 -6.157664654153905E-4 1103 58-59 -0.07683826441309805 1103 60-61 -0.09528226245488725 1103 62-63 -0.1457622783452237 1103 64-65 -0.14026265769073376 1103 66-67 0.009699218633208773 1103 68-69 -0.0242629997545194 1103 70-71 -0.01496572244833061 1103 72-73 0.12725866524213814 1103 74-75 0.013130458283693969 1103 76-77 0.11486943646431769 1103 78-79 0.07979920471157698 1103 80-81 0.0721237054786883 1103 82-83 0.017879572641110286 1103 84-85 0.15721111537146726 1103 86-87 0.06727099501647871 1103 88-89 0.06486503602916827 1103 90-91 -0.0695658394159544 1103 92-93 0.21963120576357298 1103 94-95 0.1307414125033226 1103 96-97 0.12667132227984013 1103 98-99 0.19303058426477904 1103 100-101 0.4438245749789971 1104 1 0.14552859830835274 1104 2 0.18622074605804784 1104 3 0.07486816341967995 1104 4 0.0065323711586984246 1104 5 0.02799817695570539 1104 6 0.11876390287051208 1104 7 0.0683089048181742 1104 8 0.07159302737715478 1104 9 0.1439818645411819 1104 10-11 0.08029749020175814 1104 12-13 0.08942153525742924 1104 14-15 0.1358101815651267 1104 16-17 0.2335774730847291 1104 18-19 0.14844710502118375 1104 20-21 -0.038114413111735246 1104 22-23 0.020949650739176207 1104 24-25 0.07092597807545786 1104 26-27 0.005852316591692386 1104 28-29 0.11711486898291668 1104 30-31 0.10731279611642464 1104 32-33 0.0055814776692670875 1104 34-35 0.12220705986980107 1104 36-37 0.06721744336486069 1104 38-39 0.07322551084914863 1104 40-41 0.10181057612139455 1104 42-43 0.14387023732830784 1104 44-45 0.10952035435564511 1104 46-47 -0.020607381289039495 1104 48-49 -0.1634402059054807 1104 50-51 -0.14041446102861244 1104 52-53 -0.03525374295814743 1104 54-55 0.0930493650134494 1104 56-57 0.04011496290014804 1104 58-59 0.11199104113488545 1104 60-61 0.1745937217612905 1104 62-63 0.0323162952914231 1104 64-65 -0.09403359217329665 1104 66-67 -0.019226553737823338 1104 68-69 -0.12618733557907547 1104 70-71 0.02335260897567082 1104 72-73 0.08664831483390145 1104 74-75 -0.09222322309393505 1104 76-77 -0.026322260660773367 1104 78-79 -0.08240680332581007 1104 80-81 0.05813919835960135 1104 82-83 0.06508321674126805 1104 84-85 0.2078275574640962 1104 86-87 0.18266159018313743 1104 88-89 0.188183731135819 1104 90-91 0.10122107891910304 1104 92-93 0.03839704868903482 1104 94-95 0.04121701848953663 1104 96-97 0.13527150775799157 1104 98-99 0.11426019530618703 1104 100-101 0.5282899614827308 1105 1 0.12629751940335865 1105 2 0.1957618088599773 1105 3 0.13601148064995527 1105 4 0.12114589772875206 1105 5 0.048863716408206415 1105 6 0.07380013475457048 1105 7 0.17086123976181256 1105 8 0.14920172302932855 1105 9 0.18288283072475764 1105 10-11 0.19682728086198864 1105 12-13 0.09784142253602113 1105 14-15 0.16944142150071428 1105 16-17 0.2631326260637934 1105 18-19 0.2191536267603169 1105 20-21 0.24508606998005433 1105 22-23 0.22392469099682444 1105 24-25 0.18370778161813206 1105 26-27 0.21682856457881172 1105 28-29 0.164419619385491 1105 30-31 0.2517491890310808 1105 32-33 0.12705088185605717 1105 34-35 0.12527066695515288 1105 36-37 0.08371905367081212 1105 38-39 0.0989682644723402 1105 40-41 0.1770621864273565 1105 42-43 0.1044620247679191 1105 44-45 0.18905739139267297 1105 46-47 0.1314598489847114 1105 48-49 0.20364514031030723 1105 50-51 0.29888706876204196 1105 52-53 0.31976638586632333 1105 54-55 0.3408674004401817 1105 56-57 0.42603887594362533 1105 58-59 0.3562865322782045 1105 60-61 0.4389979085567788 1105 62-63 0.35661178643474045 1105 64-65 0.22021761555617303 1105 66-67 0.3079554108354472 1105 68-69 0.26693867086214595 1105 70-71 0.387344154869389 1105 72-73 0.2423467850432388 1105 74-75 0.32730978817820855 1105 76-77 0.1955336169559736 1105 78-79 0.36673168298658965 1105 80-81 0.2339141581019497 1105 82-83 0.3003851491084184 1105 84-85 0.3872961564979107 1105 86-87 0.45935241143917693 1105 88-89 0.43619299039507453 1105 90-91 0.47894652175324026 1105 92-93 0.5206756316197918 1105 94-95 0.4178353759774609 1105 96-97 0.5008612823151566 1105 98-99 0.5593527878987778 1105 100-101 0.9833161289545522 1106 1 -0.13662283373157535 1106 2 0.04226588791318164 1106 3 0.021667767754017575 1106 4 0.04734663310735243 1106 5 -0.023651029464865303 1106 6 -0.043293108954493675 1106 7 0.01558240870738814 1106 8 -0.014060945016176163 1106 9 -0.07977516077242797 1106 10-11 -0.07748138664730675 1106 12-13 -0.18555103791823058 1106 14-15 -0.18893308537430897 1106 16-17 0.004131180202215035 1106 18-19 0.01713061745318356 1106 20-21 0.036165295534459574 1106 22-23 0.03918331829009247 1106 24-25 0.21396698342594078 1106 26-27 0.22029456289357086 1106 28-29 0.20672280028097134 1106 30-31 0.07323699017245389 1106 32-33 0.07184625892909935 1106 34-35 -0.003443090752917044 1106 36-37 0.14560240806967784 1106 38-39 0.17716328343131238 1106 40-41 0.15065055633811397 1106 42-43 -0.014360507692153135 1106 44-45 0.11878612477963202 1106 46-47 0.056845970628593534 1106 48-49 0.13843965561154192 1106 50-51 0.14506056257643962 1106 52-53 0.04777444822030219 1106 54-55 -0.16421896824494553 1106 56-57 -0.07187348186403142 1106 58-59 -0.05372677730852615 1106 60-61 3.7808397521388315E-4 1106 62-63 0.042746356889374226 1106 64-65 0.02580476941115961 1106 66-67 -0.013664528649023566 1106 68-69 0.11041960655482086 1106 70-71 0.04706422266210808 1106 72-73 0.07306057147704337 1106 74-75 -0.05433351489736182 1106 76-77 -0.15430504021205138 1106 78-79 -0.09472796694254981 1106 80-81 -0.04644917446536212 1106 82-83 -0.06894582871531796 1106 84-85 -0.1363049006088346 1106 86-87 0.12097484048662821 1106 88-89 0.05619303827128874 1106 90-91 -0.07164187688324475 1106 92-93 0.12663813497112741 1106 94-95 -0.2871313021794961 1106 96-97 0.026288098738611154 1106 98-99 0.23042358724000067 1106 100-101 0.5995689057748059 1107 1 0.130866081765042 1107 2 0.19822015785158698 1107 3 0.16081836193936283 1107 4 0.14021454360122476 1107 5 0.05363270467493919 1107 6 0.10604834971135801 1107 7 0.09732267865279454 1107 8 0.10311942314206846 1107 9 0.15928111457794358 1107 10-11 0.17629719609853112 1107 12-13 0.21121384935968024 1107 14-15 0.18757161482820095 1107 16-17 0.03165036166862478 1107 18-19 0.1761928037614453 1107 20-21 0.23014964257180992 1107 22-23 0.2443154171231825 1107 24-25 0.27010126360067943 1107 26-27 0.18670178475501586 1107 28-29 0.06912310387336618 1107 30-31 0.1879250210073664 1107 32-33 0.15150545198424936 1107 34-35 0.15489246744786556 1107 36-37 0.17881540424912856 1107 38-39 0.22574156398379586 1107 40-41 0.2776475448974338 1107 42-43 0.28442413174524717 1107 44-45 0.36917086168371327 1107 46-47 0.2505945205785167 1107 48-49 0.12099241994619803 1107 50-51 0.2276312230064832 1107 52-53 0.3177647365281473 1107 54-55 0.21379320109992506 1107 56-57 0.27301604618037345 1107 58-59 0.31608495809973647 1107 60-61 0.4073504493726787 1107 62-63 0.35020389884027026 1107 64-65 0.40702395402541214 1107 66-67 0.47626410848462086 1107 68-69 0.3345069931302973 1107 70-71 0.11743231095106665 1107 72-73 0.182599248611659 1107 74-75 0.20359585440226624 1107 76-77 0.262334422835135 1107 78-79 0.38963451817804184 1107 80-81 0.35725933952915057 1107 82-83 0.3141597325983341 1107 84-85 0.32091760206975195 1107 86-87 0.18709887266929215 1107 88-89 0.36181958231958333 1107 90-91 0.3452118146673513 1107 92-93 0.5847315420962182 1107 94-95 0.43035260007867393 1107 96-97 0.35603764667274973 1107 98-99 0.6406187071438403 1107 100-101 1.1968216511057896 1108 1 -0.03437785025688811 1108 2 0.040202702672409885 1108 3 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46-47 -0.5955227162293752 2316 48-49 -0.4514388964985798 2316 50-51 -0.4529386404173579 2316 52-53 -0.36535700754762956 2316 54-55 -0.2409867329539921 2316 56-57 -0.34103419044925687 2316 58-59 -0.30429887345607654 2316 60-61 -0.41357072784142446 2316 62-63 -0.40652324284504715 2316 64-65 -0.5111250721006897 2316 66-67 -0.38878388492849325 2316 68-69 -0.18901282735064484 2316 70-71 -0.4565313299583522 2316 72-73 -0.2533292169340946 2316 74-75 -0.2762018857133448 2316 76-77 -0.21346611686408679 2316 78-79 -0.2657879002516772 2316 80-81 -0.35711985589150785 2316 82-83 -0.4375882709235235 2316 84-85 -0.45080560028967653 2316 86-87 -0.5616149625056117 2316 88-89 -0.5487806768039469 2316 90-91 -0.41559393759727925 2316 92-93 -0.41790715034081316 2316 94-95 -0.3175228261671883 2316 96-97 -0.3453537340358963 2316 98-99 -0.26415185123727625 2316 100-101 -0.4960934269052082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 22.0 5 63.0 6 98.0 7 259.0 8 614.0 9 1220.0 10 2103.0 11 3868.0 12 6148.0 13 8536.0 14 10773.0 15 15518.0 16 21722.0 17 31799.0 18 26966.0 19 14311.0 20 10854.0 21 10180.0 22 10687.0 23 11159.0 24 12830.0 25 15155.0 26 18415.0 27 22801.0 28 28549.0 29 36068.0 30 44921.0 31 57619.0 32 73804.0 33 97421.0 34 135355.0 35 192920.0 36 297064.0 37 469372.0 38 426112.0 39 44374.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.129257815106115 20.49343397653059 15.892677113988984 20.484631094374308 2 15.220255026042956 22.420696198223737 39.724202563225184 22.63484621250812 3 19.379164060210186 26.758379979450858 31.09638787961852 22.766068080720434 4 12.263966169391042 18.29919975033581 38.95511462003305 30.481719460240097 5 12.219451833519809 39.4014107988352 34.33894738402033 14.040189983624657 6 29.05267905140573 38.860564494807385 17.56122722543537 14.525529228351516 7 26.320912015135427 35.06485388486647 21.294197669029185 17.320036430968916 8 26.0695346253949 34.039480465119944 20.14709443575754 19.743890473727614 9 26.611646704798485 17.07421536592334 21.240116108665042 35.07402182061313 10-11 24.24750000115757 27.73670985011351 27.43828891128911 20.57750123743981 12-13 26.29954288762407 25.725721461500132 27.771660514418116 20.203075136457677 14-15 22.74715342540472 26.668460124804312 28.11039583720154 22.47399061258943 16-17 20.368861245144583 29.80850126938868 28.42879639357485 21.39384109189189 18-19 20.230184641299882 28.115442832132782 31.8473251621405 19.807047364426836 20-21 21.990358850600153 26.383814240952795 31.766573243240614 19.859253665206435 22-23 21.120900613654875 26.700469467046783 31.75804041611662 20.420589503181716 24-25 21.139159271342567 26.875447400516183 31.973081421569127 20.012311906572123 26-27 21.013398083831614 26.642255580644214 32.43535079013064 19.90899554539353 28-29 20.299467895086682 27.69964584219384 32.02999309372599 19.97089316899349 30-31 20.851566953018033 26.823875156792536 31.835680031634006 20.48887785855542 32-33 20.637995722556038 27.11213265169577 31.761989275367284 20.48788235038091 34-35 21.353025256736927 26.981952594363754 31.722863488973708 19.94215865992561 36-37 21.148344145786847 26.602224104169974 31.946968585003198 20.302463165039974 38-39 20.633272846565333 27.406270590234485 31.76486004312634 20.195596520073845 40-41 20.991123308272766 27.384948194217625 31.409579381590326 20.214349115919287 42-43 20.733469588961672 27.530753028067934 30.75386162451379 20.981915758456605 44-45 21.21432550153933 27.095278466787764 30.660031180242083 21.03036485143082 46-47 21.72499804371068 27.799496319166405 30.035917008882247 20.439588628240667 48-49 21.520779959818512 27.924027446391886 29.587753119529047 20.96743947426056 50-51 21.058771561433737 28.23013463437763 29.835171627924424 20.875922176264215 52-53 21.587154888570222 28.81702143028132 28.584280879621765 21.01154280152669 54-55 21.317557384100855 28.20987720059397 28.902195257584268 21.570370157720905 56-57 21.81385293612403 28.296972590187252 28.48996226792505 21.399212205763668 58-59 22.241736240340675 28.00246423000221 28.60951585427798 21.146283675379138 60-61 21.744954509906695 28.36082402147334 29.33521816214374 20.55900330647622 62-63 21.428799634764257 28.263310523077035 29.26129589234806 21.046593949810646 64-65 21.254909823165335 28.822485149564685 28.858531806023148 21.06407322124683 66-67 21.590025656329278 28.333598030468103 28.417822652302334 21.65855366090028 68-69 21.60130036518944 28.33341281964494 28.579349641455 21.48593717371062 70-71 21.925928635491672 28.255161246857785 28.561777764607232 21.257132353043314 72-73 21.77536138775119 27.89363130126446 29.046224340422068 21.284782970562286 74-75 21.260095726213954 29.112849417581415 28.357953253714285 21.269101602490345 76-77 21.225391848223435 28.223536498802382 28.719299569708955 21.83177208326523 78-79 21.27329199736445 28.42541629605209 28.576062149343823 21.725229557239636 80-81 21.537865658255473 28.552957099154003 28.386961898892487 21.52221534369804 82-83 21.469608931252857 28.312652191487974 28.671290463430747 21.54644841382842 84-85 21.14414027302269 28.444385587824826 28.491198208618524 21.92027593053396 86-87 21.021706245447866 28.49193013292118 28.307390698990275 22.17897292264068 88-89 21.04219519276048 28.233792548135135 28.138038552558896 22.585973706545488 90-91 21.204218847932125 27.644600056633493 28.429133695099257 22.722047400335125 92-93 21.48663897425402 27.986569287907404 28.52169560540766 22.005096132430914 94-95 21.040551446704892 27.86679730203394 28.71865133182788 22.37399991943329 96-97 20.435676049601312 28.062981866471333 29.114701525813064 22.386640558114294 98-99 20.647822206582173 28.313247572866107 28.178483329262548 22.860446891289175 100-101 20.324059667100112 28.048556853418095 28.614882136546004 23.01250134293579 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 853.0 1 633.0 2 432.5 3 502.5 4 492.5 5 509.0 6 868.5 7 1831.5 8 2975.5 9 3665.5 10 3602.5 11 3340.0 12 3511.5 13 3787.0 14 4019.0 15 4269.0 16 4546.0 17 4930.0 18 5284.0 19 5642.0 20 6124.5 21 6932.5 22 7981.0 23 9154.5 24 10821.5 25 13137.5 26 16318.0 27 19653.5 28 22397.0 29 25944.0 30 30756.5 31 34996.5 32 39486.0 33 45725.0 34 53215.5 35 58680.5 36 64850.5 37 70803.0 38 72402.5 39 72628.5 40 72840.0 41 73414.5 42 73526.0 43 75238.0 44 77918.5 45 78723.5 46 77902.0 47 86163.5 48 104767.5 49 99706.5 50 77751.0 51 66002.0 52 59081.5 53 54742.5 54 50489.0 55 45573.0 56 40623.5 57 36213.0 58 33362.0 59 30378.0 60 26239.0 61 21584.5 62 16944.5 63 12029.5 64 8100.5 65 6109.0 66 4406.0 67 3192.0 68 2287.0 69 1579.0 70 1208.5 71 916.0 72 758.5 73 748.0 74 587.0 75 443.5 76 340.5 77 199.5 78 135.0 79 85.5 80 43.5 81 28.5 82 20.0 83 11.5 84 5.5 85 3.0 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06098066352703453 2 0.0 3 0.0 4 0.0 5 0.01653006596746494 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002639254230099444 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.334487042419297E-4 24-25 1.3890811737365496E-4 26-27 0.0012270217034672855 28-29 0.0032643407582808918 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01294160626864552 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.5466488185170075E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8521082316487328E-4 84-85 0.0012501730563628946 86-87 0.0 88-89 0.0 90-91 0.009329995216930492 92-93 0.0011112649389892397 94-95 0.0 96-97 0.0 98-99 1.6205947026926414E-4 100-101 0.011714584565178236 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2159701.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.15446610803846 #Duplication Level Percentage of deduplicated Percentage of total 1 68.5184518552251 30.939141120720272 2 15.659403544469482 14.141840132416892 3 6.283568492727065 8.511935416271479 4 3.1648539239184017 5.71629156977864 5 1.769489279036785 3.995017183940197 6 1.1249427684598077 3.0477714067140806 7 0.7388957616621434 2.3355110539142565 8 0.5072335684779716 1.832308878135838 9 0.3697268362567188 1.5025336107287668 >10 1.6817508556483167 13.322662104559788 >50 0.11195824801618534 3.4971743547536094 >100 0.06456969752162746 5.27800475736226 >500 0.0021651708038063003 0.6933086309321431 >1k 0.002371377547025936 2.254277277623232 >5k 3.093101148294699E-4 0.9265530736510516 >10k+ 3.093101148294699E-4 2.0056694284976864 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16024 0.7419545575984824 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13911 0.644116940261638 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13146 0.608695370331356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8800 0.4074638109627212 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5829 0.2698984720570116 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5273 0.24415416763709422 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3928 0.18187702834790556 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3914 0.1812287904668285 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3906 0.18085836882049877 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3277 0.15173396687782242 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3088 0.14298275548328215 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3053 0.14136216078058952 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2906 0.13455566302928043 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2885 0.13358330620766484 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2312 0.10705185578929677 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.315135289560916E-5 0.0 16-17 0.0 0.0 0.0 4.630270579121832E-5 0.0 18-19 0.0 0.0 0.0 4.630270579121832E-5 0.0 20-21 0.0 0.0 0.0 6.945405868682748E-5 0.0 22-23 0.0 0.0 0.0 1.620594702692641E-4 0.0 24-25 0.0 4.630270579121832E-5 0.0 4.630270579121832E-4 0.0 26-27 0.0 9.260541158243664E-5 0.0 9.029027629287573E-4 0.0 28-29 0.0 9.260541158243664E-5 0.0 0.0021993785250828705 0.0 30-31 0.0 9.260541158243664E-5 0.0 0.005648930106528635 0.0 32-33 0.0 9.260541158243664E-5 0.0 0.009306843864034884 0.0 34-35 0.0 9.260541158243664E-5 0.0 0.014214930677904025 0.0 36-37 0.0 9.260541158243664E-5 0.0 0.02099827707631751 0.0 38-39 0.0 9.260541158243664E-5 0.0 0.03227298593647917 0.0 40-41 0.0 9.260541158243664E-5 0.0 0.0432235758561023 0.0 42-43 0.0 9.260541158243664E-5 0.0 0.053850046835186904 0.0 44-45 0.0 9.260541158243664E-5 0.0 0.06195302034865011 0.0 46-47 0.0 9.260541158243664E-5 0.0 0.07422323738332297 0.0 48-49 0.0 9.260541158243664E-5 0.0 0.08394680559947881 0.0 50-51 0.0 9.260541158243664E-5 0.0 0.09413340087354685 0.0 52-53 0.0 9.260541158243664E-5 0.0 0.11874328900157938 0.0 54-55 0.0 9.260541158243664E-5 0.0 0.1599526971557637 0.0 56-57 0.0 9.260541158243664E-5 0.0 0.22357261491289765 0.0 58-59 0.0 9.260541158243664E-5 0.0 0.3047181068120078 0.0 60-61 0.0 9.260541158243664E-5 0.0 0.41003361113413384 0.0 62-63 0.0 9.260541158243664E-5 0.0 0.47119948548433327 0.0 64-65 0.0 9.260541158243664E-5 0.0 0.5553546532598725 0.0 66-67 0.0 9.260541158243664E-5 0.0 0.7175530316465104 0.0 68-69 0.0 9.260541158243664E-5 0.0 0.911214098618281 0.0 70-71 0.0 9.260541158243664E-5 0.0 1.1146681878648943 0.0 72-73 0.0 9.260541158243664E-5 0.0 1.3630822044347806 0.0 74-75 0.0 9.260541158243664E-5 0.0 1.6075604910124133 0.0 76-77 0.0 9.260541158243664E-5 0.0 1.8425235715499508 0.0 78-79 0.0 9.260541158243664E-5 0.0 2.0766069006774552 0.0 80-81 0.0 9.260541158243664E-5 0.0 2.320205435845055 0.0 82-83 0.0 9.260541158243664E-5 0.0 2.6372863651033174 0.0 84-85 0.0 9.260541158243664E-5 0.0 3.100660693308935 0.0 86-87 0.0 9.260541158243664E-5 0.0 3.658654600798907 0.0 88-89 0.0 9.260541158243664E-5 0.0 4.299391443537786 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 13445 0.0 24.323593 12-13 GTACATG 13290 0.0 23.388853 1 ATGGGCG 265 3.092282E-11 23.304642 5 GTACTTT 14050 0.0 23.17425 14-15 TACATGG 13190 0.0 23.014471 2 ACATGGG 13235 0.0 21.967085 3 AGTACTT 13915 0.0 21.86354 12-13 AGAGTAC 21710 0.0 20.62083 10-11 ACTTTTT 16245 0.0 20.364603 16-17 TACTTTT 15545 0.0 19.55527 14-15 GTATCAA 32345 0.0 19.293621 1 CATGGGA 8365 0.0 18.513845 4 CATGGGG 4345 0.0 18.040108 4 GGTATCA 26035 0.0 17.543732 1 CATGGGC 2310 0.0 17.480421 4 CAACGCA 36400 0.0 17.018496 5 TCAACGC 36400 0.0 16.992393 4 ATCAACG 36635 0.0 16.89636 3 AACGCAG 37000 0.0 16.726969 6 TATCAAC 37275 0.0 16.64449 2 >>END_MODULE