FastQCFastQC Report
Thu 26 May 2016
SRR937424_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937424_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270754
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152320.6707904070630284No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT112840.4969274522911773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111390.4905419081062942No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT79140.3485185977873429No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74740.32914177405390455No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63090.2778372293960508No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44060.19403246674893007No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37860.1667287605790852No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37280.16417454290513195No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33460.14735193684564687No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32130.14149485148985755No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC30780.1355496896625526No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29940.1318504778588962No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.11996015420428632No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA25400.11185711882484847No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23540.10366600697389501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139550.026.2864231
TACATGG138950.025.6094442
ACATGGG139450.024.8016263
GTAAGCG2050.023.1687894-95
GAGTACT122450.022.94314812-13
AGTACTT127700.022.70658312-13
CATGGGG46200.022.3153764
AGAGTAC202050.021.56819710-11
GTATCAA285950.021.170121
CATGGGA86750.020.866134
GTACTTT134300.020.86524214-15
CATGGGT21800.019.3963494
GTGTAGC14650.019.136321
TACTTTT151900.019.10428814-15
CAACGCA325500.018.4631925
TCAACGC325900.018.4413474
TAGCCCT17550.018.4084954
ATCAACG327450.018.3532433
ACTTTTT155450.018.30076216-17
AACGCAG330550.018.2078556
Produced by FastQC (version 0.11.5)