FastQCFastQC Report
Thu 26 May 2016
SRR937424_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937424_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270754
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167460.7374642960003593No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145960.6427820891210584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135200.5953969474456502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92940.4092913631331267No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT60310.2655945998553784No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58040.25559792033835455No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41170.18130541661492172No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC40640.17897139011975757No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC39720.17491987243003865No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34020.14981807804808447No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33490.14748405155292033No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.13823602204377927No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA30430.13400835141102913No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29930.1318064396231384No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC24780.109126748207864No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA22810.10045121576357458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG142200.023.0991941
GAGTACT141300.023.04504212-13
TACATGG142600.022.3499722
TGTACCG1503.035955E-522.1656635
AGTACTT146400.021.8366612-13
GTACTTT150100.021.48637214-15
ACATGGG142850.021.08063
AGAGTAC232100.020.50712610-11
GTATCAA331100.019.3817481
TACTTTT166600.019.13028714-15
TACGACG1759.945652E-518.9991425
ACTTTTT170800.018.95186616-17
CATGGGG47400.018.4379844
ACCGTCG1300.007563471318.2700168
CATGGGA86950.017.8629074
CGACGGT1600.001191158317.8132637
ATGGGAT38400.016.9458485
CAACGCA377000.016.9329225
TCAACGC377650.016.9163554
ATCAACG378550.016.8761353
Produced by FastQC (version 0.11.5)