##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937424_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2270754 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22825237784454 33.0 31.0 34.0 31.0 34.0 2 32.414370733245434 34.0 31.0 34.0 31.0 34.0 3 32.460184590669 34.0 31.0 34.0 31.0 34.0 4 36.105172114636815 37.0 35.0 37.0 35.0 37.0 5 36.042201841326715 37.0 35.0 37.0 35.0 37.0 6 36.103075013850024 37.0 35.0 37.0 35.0 37.0 7 36.05023397514658 37.0 35.0 37.0 35.0 37.0 8 36.03659357200296 37.0 35.0 37.0 35.0 37.0 9 37.73723529717442 39.0 37.0 39.0 35.0 39.0 10-11 37.66055834317588 39.0 37.0 39.0 35.0 39.0 12-13 37.57735536302039 39.0 37.0 39.0 35.0 39.0 14-15 38.725772804980195 40.0 38.0 41.0 34.5 41.0 16-17 38.72572920712679 40.0 38.0 41.0 34.5 41.0 18-19 38.7798361249171 40.0 38.0 41.0 35.0 41.0 20-21 38.71197760743788 40.0 38.0 41.0 34.5 41.0 22-23 38.56520609453952 40.0 38.0 41.0 34.0 41.0 24-25 38.49039438001651 40.0 38.0 41.0 34.0 41.0 26-27 38.42373942752055 40.0 38.0 41.0 34.0 41.0 28-29 38.31223791744945 40.0 38.0 41.0 34.0 41.0 30-31 38.32761342708193 40.0 38.0 41.0 34.0 41.0 32-33 38.206080447287555 40.0 37.0 41.0 34.0 41.0 34-35 38.04020228523213 40.0 37.0 41.0 34.0 41.0 36-37 37.85150350940701 40.0 37.0 41.0 33.0 41.0 38-39 37.70447327187357 40.0 37.0 41.0 33.0 41.0 40-41 37.5220420177615 40.0 37.0 41.0 33.0 41.0 42-43 37.264897474583336 40.0 36.5 41.0 32.5 41.0 44-45 36.88497499068591 40.0 36.0 41.0 31.5 41.0 46-47 36.41242556437201 40.0 36.0 41.0 30.5 41.0 48-49 35.983902483492265 39.0 36.0 41.0 29.5 41.0 50-51 35.700895385409424 39.0 35.0 41.0 28.0 41.0 52-53 35.476008629732675 39.0 35.0 41.0 27.5 41.0 54-55 35.26071252103927 39.0 35.0 40.0 27.0 41.0 56-57 34.991195875907295 39.0 35.0 40.0 26.0 41.0 58-59 34.74632853228487 38.0 34.5 40.0 26.0 41.0 60-61 34.4155337830518 38.0 34.0 40.0 25.0 41.0 62-63 34.08299952350629 38.0 34.0 40.0 23.5 41.0 64-65 33.716714139884814 37.0 33.5 40.0 22.5 41.0 66-67 33.30778014703486 36.5 33.0 39.0 22.0 41.0 68-69 32.876053504694916 36.0 33.0 39.0 20.5 40.0 70-71 32.466622540354436 35.5 32.0 39.0 19.5 40.0 72-73 32.00330506959362 35.0 32.0 38.0 18.0 40.0 74-75 31.541476311392604 35.0 31.0 37.0 16.0 39.0 76-77 30.267230400122603 34.0 29.5 36.0 13.0 38.5 78-79 30.852282105415206 34.5 31.0 36.0 12.0 39.0 80-81 30.64510576663082 34.5 31.0 36.0 7.5 37.5 82-83 30.297825303841808 34.0 31.0 35.5 6.5 37.0 84-85 29.940533408726793 34.0 30.5 35.0 2.0 37.0 86-87 29.57208949097965 34.0 30.0 35.0 2.0 36.0 88-89 29.114809442150055 34.0 29.5 35.0 2.0 36.0 90-91 28.632958259679384 34.0 29.0 35.0 2.0 35.0 92-93 28.323951647778667 34.0 29.0 35.0 2.0 35.0 94-95 27.913351908661177 34.0 29.0 35.0 2.0 35.0 96-97 27.482228369959934 33.5 27.0 35.0 2.0 35.0 98-99 26.825612549840272 33.0 26.5 35.0 2.0 35.0 100-101 24.450416909977918 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.029811869802891522 1101 2 -0.025222123557050224 1101 3 0.016941172163463136 1101 4 -0.013636516457545156 1101 5 -0.0739190134441472 1101 6 -0.10294734933506788 1101 7 -0.048462874682208223 1101 8 -0.057486947573409 1101 9 -0.07009527397448778 1101 10-11 -0.12652571086011477 1101 12-13 -0.12250104428813557 1101 14-15 -0.2577899581127525 1101 16-17 -0.18235632441400895 1101 18-19 -0.17728105618192558 1101 20-21 -0.01891376390078392 1101 22-23 -0.08429376105497965 1101 24-25 -0.09805289559186292 1101 26-27 -0.2249475780037855 1101 28-29 -0.23697704329278935 1101 30-31 -0.10409676570570525 1101 32-33 -0.0850239765502181 1101 34-35 -0.05787885928680225 1101 36-37 -0.07664111767814319 1101 38-39 -0.04172608258250676 1101 40-41 0.056040880111417835 1101 42-43 -0.07331906123300058 1101 44-45 0.02645573923226152 1101 46-47 -0.024822343783121426 1101 48-49 -0.056864345547317896 1101 50-51 0.02401105340364751 1101 52-53 0.08373402005975805 1101 54-55 0.048922938705210584 1101 56-57 0.14031924952899288 1101 58-59 0.08642571814489486 1101 60-61 0.007382765251023216 1101 62-63 -0.02632306924130745 1101 64-65 -0.08895974663094819 1101 66-67 0.10235852971316461 1101 68-69 0.07203477395292168 1101 70-71 0.08974363752991366 1101 72-73 0.201486621507442 1101 74-75 0.08979969471776172 1101 76-77 0.1549403474269404 1101 78-79 -0.013487264618724737 1101 80-81 0.0027562486654950646 1101 82-83 0.06927394814949395 1101 84-85 0.18695306353497898 1101 86-87 0.06874469086400836 1101 88-89 0.11626201284686388 1101 90-91 0.08450776771005764 1101 92-93 0.01973110779992382 1101 94-95 -0.019795682724030428 1101 96-97 0.0030253425881028306 1101 98-99 0.2677468466309634 1101 100-101 0.7654182676112562 1102 1 -0.11056713337662671 1102 2 -0.08708054159122014 1102 3 -0.06159648546218932 1102 4 -0.08420138518355458 1102 5 0.023203916023355475 1102 6 -0.055315892398098754 1102 7 -0.15857416057117035 1102 8 -0.09351997438537296 1102 9 -0.2334971973467077 1102 10-11 -0.1481273063357449 1102 12-13 -0.15584042221735928 1102 14-15 -0.23532148820504517 1102 16-17 -0.215441680475017 1102 18-19 -0.18636829160786306 1102 20-21 -0.11215337126831315 1102 22-23 -0.1069115680375603 1102 24-25 -0.20411310323985532 1102 26-27 -0.16851435015733074 1102 28-29 -0.2496061348991745 1102 30-31 -0.23603514109198187 1102 32-33 -0.2005071179572937 1102 34-35 -0.10555031722596198 1102 36-37 -0.21638948718768347 1102 38-39 -0.10614028558021715 1102 40-41 -0.027605866759010667 1102 42-43 -0.08450954315267012 1102 44-45 0.005806229625939352 1102 46-47 0.16985056461098935 1102 48-49 0.0982571223021651 1102 50-51 0.14330793377200024 1102 52-53 0.2051012778812904 1102 54-55 0.26856738747154907 1102 56-57 0.1451391532338704 1102 58-59 0.133831432991947 1102 60-61 0.1987575445013121 1102 62-63 0.2033623132637814 1102 64-65 0.09124464422635015 1102 66-67 0.2571839901464088 1102 68-69 0.19495574512020397 1102 70-71 0.14072296129095463 1102 72-73 0.143479462754442 1102 74-75 0.2322685664960069 1102 76-77 0.2161102520868745 1102 78-79 0.07135101598067806 1102 80-81 0.0323190358509855 1102 82-83 -0.01223414048726923 1102 84-85 0.02531744798219293 1102 86-87 0.048532091428953095 1102 88-89 0.2079207433973842 1102 90-91 0.00567968420334708 1102 92-93 0.18826711329116463 1102 94-95 0.17678468431276428 1102 96-97 0.12138750186328551 1102 98-99 0.3501868655933009 1102 100-101 0.9930825142069537 1103 1 0.0572025338051958 1103 2 0.047860834387414286 1103 3 -0.007623251534326414 1103 4 -0.018595378846896438 1103 5 -0.040005392626646596 1103 6 -0.030698059552285883 1103 7 -0.0979903855954376 1103 8 -0.10565159819227432 1103 9 -0.14788379529624507 1103 10-11 -0.1041381478552168 1103 12-13 -0.09967500585745626 1103 14-15 -0.11669172382872262 1103 16-17 -0.15613211844720354 1103 18-19 -0.1362627451541556 1103 20-21 -0.20698446930519054 1103 22-23 -0.1131908772845236 1103 24-25 -0.20296809418591266 1103 26-27 -0.10016991219841742 1103 28-29 -0.3198984424724358 1103 30-31 -0.2583076814367544 1103 32-33 -0.11522508589371938 1103 34-35 -0.016914787730442526 1103 36-37 -0.11244488539546182 1103 38-39 -0.04547194078297423 1103 40-41 0.07383190311821153 1103 42-43 0.09182447668862181 1103 44-45 0.04015055924704569 1103 46-47 0.029014312789691132 1103 48-49 -0.17322706701040858 1103 50-51 -0.03760750414490133 1103 52-53 0.056998792523479835 1103 54-55 -0.030192918439041705 1103 56-57 -0.14719370805614318 1103 58-59 -0.04261175377580173 1103 60-61 0.020185049923725273 1103 62-63 0.15941778885571267 1103 64-65 -0.049651442125515644 1103 66-67 0.19639929275160029 1103 68-69 0.14732773515311948 1103 70-71 0.11853540532563756 1103 72-73 0.23255774013902908 1103 74-75 0.3508782939048096 1103 76-77 0.42477661363889396 1103 78-79 0.21997432430787356 1103 80-81 0.09512497961702948 1103 82-83 0.17650055126955166 1103 84-85 0.14162029859319603 1103 86-87 0.24136551762326874 1103 88-89 0.21566731141031426 1103 90-91 0.10161184815162017 1103 92-93 0.09578158412728754 1103 94-95 0.18722270071377878 1103 96-97 0.33465214018082534 1103 98-99 0.458924310014897 1103 100-101 1.0565999775987223 1104 1 0.015631608137034902 1104 2 0.08601362229164522 1104 3 0.04584073143850276 1104 4 0.08217110149305284 1104 5 0.025047854097905997 1104 6 -0.0010192706022706943 1104 7 0.0143055203721687 1104 8 0.04335453659632549 1104 9 0.035832133796496635 1104 10-11 0.004276105033582667 1104 12-13 -0.0304579462844643 1104 14-15 0.015205770702991117 1104 16-17 -0.19623608948923277 1104 18-19 -0.11788461608055911 1104 20-21 -0.03881586572446594 1104 22-23 -0.07248300148269493 1104 24-25 0.007688799534214752 1104 26-27 -0.15379821986579856 1104 28-29 -0.15398435528921084 1104 30-31 -0.0928276507996415 1104 32-33 0.0057280157463921455 1104 34-35 0.03784998400092121 1104 36-37 -0.14675146280205809 1104 38-39 -0.16388974713576232 1104 40-41 -0.23545801167229996 1104 42-43 -0.17385357210982733 1104 44-45 -0.09734594252416429 1104 46-47 -0.03748103272483405 1104 48-49 0.014146812635786432 1104 50-51 -0.01766990149950942 1104 52-53 -0.04603601301182181 1104 54-55 -4.8006778763465263E-4 1104 56-57 0.11607056287615336 1104 58-59 0.047165224932868455 1104 60-61 0.07956011896771287 1104 62-63 0.0430031787793439 1104 64-65 0.1104882660853761 1104 66-67 0.01467551289697866 1104 68-69 0.10589121511747379 1104 70-71 0.061174119303082364 1104 72-73 0.19248000581293923 1104 74-75 0.21831291384986784 1104 76-77 0.2829179126233008 1104 78-79 0.11623849213448167 1104 80-81 0.09777212616703679 1104 82-83 0.20630453998684217 1104 84-85 0.2543976713129048 1104 86-87 0.0970741667177819 1104 88-89 0.0651785720246778 1104 90-91 0.15162777429848262 1104 92-93 0.15308855554491885 1104 94-95 0.37055198346640594 1104 96-97 0.522665367167999 1104 98-99 0.6362255922078077 1104 100-101 1.1871354643680547 1105 1 0.007615910449885632 1105 2 -0.007972308360450597 1105 3 -0.013574040428942169 1105 4 -6.081487191522683E-4 1105 5 -0.054293972616335395 1105 6 -0.07832714405723351 1105 7 -0.1412075780841775 1105 8 -0.08679526603862797 1105 9 -0.029419100123163844 1105 10-11 -0.06482674606358785 1105 12-13 -0.10079978313088844 1105 14-15 -0.08469024372261913 1105 16-17 -0.1353049226119154 1105 18-19 -0.09196494922660747 1105 20-21 -0.06710515512859416 1105 22-23 -0.06697624380944944 1105 24-25 -0.20882859725401914 1105 26-27 -0.0801029585552655 1105 28-29 -0.13010689451789403 1105 30-31 -0.16746479621830446 1105 32-33 -0.2493644665361856 1105 34-35 -0.23209659980413022 1105 36-37 -0.2914647204970464 1105 38-39 -0.31193771744781884 1105 40-41 -0.34199852943972786 1105 42-43 -0.253193764601626 1105 44-45 -0.34710481300189855 1105 46-47 -0.4259854707827415 1105 48-49 -0.2701837886044629 1105 50-51 -0.34087783767593294 1105 52-53 -0.21863188385464838 1105 54-55 -0.11604165689247736 1105 56-57 -0.29006166075476614 1105 58-59 -0.25933270499755423 1105 60-61 -0.19371725532054285 1105 62-63 -0.09413210771128888 1105 64-65 -0.12511488783321312 1105 66-67 -0.054233192573327926 1105 68-69 -0.10501630456101196 1105 70-71 -0.03186584168475548 1105 72-73 -0.006411076202475385 1105 74-75 0.015245821500936785 1105 76-77 0.09148656581809078 1105 78-79 -0.035378946849373705 1105 80-81 -0.14315377197710788 1105 82-83 -0.18564688177034583 1105 84-85 -0.16276310251103965 1105 86-87 -0.004192306653052924 1105 88-89 0.07920739121071207 1105 90-91 0.2427033181922269 1105 92-93 0.2254356650466498 1105 94-95 0.26667778133466413 1105 96-97 0.2586999558129648 1105 98-99 0.2496340115257567 1105 100-101 0.799824825808706 1106 1 -0.19467463927693984 1106 2 -0.03457038730600459 1106 3 -0.011063194325245718 1106 4 -0.03358913187791046 1106 5 -0.10947312620985628 1106 6 -0.13325623445097534 1106 7 -0.16867257926791268 1106 8 -0.14736655557315004 1106 9 -0.12850997174024315 1106 10-11 -0.220838823514228 1106 12-13 -0.16484897572405544 1106 14-15 -0.19945019217056625 1106 16-17 -0.11474338025274022 1106 18-19 -0.13428025721655956 1106 20-21 -0.20568421613854326 1106 22-23 -0.10624163801738717 1106 24-25 -0.21433901618922135 1106 26-27 -0.17609986805864963 1106 28-29 -0.1322196640836566 1106 30-31 -0.15721466735642053 1106 32-33 -0.26443620056670625 1106 34-35 -0.1142185219383478 1106 36-37 -0.21872761006030572 1106 38-39 -0.20313906775261614 1106 40-41 -0.20496426074964802 1106 42-43 -0.38141681659835314 1106 44-45 -0.12118101096148592 1106 46-47 -0.3795163959946919 1106 48-49 -0.49559378463919046 1106 50-51 -0.2619276325365547 1106 52-53 -0.2708037128398715 1106 54-55 -0.2989132761602207 1106 56-57 -0.3271346700893716 1106 58-59 -0.30665356325968673 1106 60-61 -0.2003072687703309 1106 62-63 -0.08544181873599399 1106 64-65 -0.13203540056673546 1106 66-67 -0.1250195911780807 1106 68-69 -0.1849175159174976 1106 70-71 -0.11080182003377104 1106 72-73 -0.02044553129999116 1106 74-75 -0.14653558422997648 1106 76-77 -0.06214790489241295 1106 78-79 -0.21646327997951786 1106 80-81 -0.3021745419966635 1106 82-83 -0.3508535696750563 1106 84-85 -0.30035686709121734 1106 86-87 -0.2578746742244391 1106 88-89 -0.03596686690777062 1106 90-91 -0.211397773002588 1106 92-93 0.03380353482555165 1106 94-95 0.0671432162531147 1106 96-97 0.02578678702158399 1106 98-99 0.27634225689921976 1106 100-101 0.898346169824535 1107 1 -0.0480103958631517 1107 2 0.034416445961255704 1107 3 0.026405078903181334 1107 4 0.010714047641599223 1107 5 -0.003385232710641617 1107 6 -0.002974043124574166 1107 7 -0.020753381027404316 1107 8 -0.015924888720228125 1107 9 -0.11714989557638944 1107 10-11 -0.022394998868335847 1107 12-13 -0.023903099351514356 1107 14-15 -0.004704082805005783 1107 16-17 0.014364827398857472 1107 18-19 0.09325426727495767 1107 20-21 0.09675862273748947 1107 22-23 0.14654892588296775 1107 24-25 0.03537101690113076 1107 26-27 -0.07383330318429415 1107 28-29 -0.1037187008602416 1107 30-31 -0.064836091735053 1107 32-33 -0.0720809948274379 1107 34-35 -0.10173092738541101 1107 36-37 -0.13139688976211517 1107 38-39 -0.20481103381452925 1107 40-41 -0.21053852658832994 1107 42-43 -0.17793386112385434 1107 44-45 -0.11048830961878053 1107 46-47 -0.21141793193706349 1107 48-49 -0.2733854170450698 1107 50-51 -0.23904112664369848 1107 52-53 -0.3296969920774231 1107 54-55 -0.23276915770471618 1107 56-57 -0.28947288953177264 1107 58-59 -0.2605484325118681 1107 60-61 -0.16181783756424295 1107 62-63 -0.09648340183376547 1107 64-65 -0.10681092618322197 1107 66-67 -0.2735421519368302 1107 68-69 -0.08010447693488487 1107 70-71 -0.08208068585731354 1107 72-73 0.041844125056869075 1107 74-75 -0.035940754071379644 1107 76-77 0.07030864614431209 1107 78-79 -0.0872630240087311 1107 80-81 -0.02099927611524066 1107 82-83 -0.21807783937604697 1107 84-85 -0.10389291454619354 1107 86-87 0.06921927309373288 1107 88-89 0.1266030123726729 1107 90-91 0.06279681890868716 1107 92-93 0.19430521875103324 1107 94-95 0.21971874586914453 1107 96-97 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58-59 -0.016929470345274922 2316 60-61 -0.025909085736714133 2316 62-63 -0.03705689996642292 2316 64-65 -0.07871516142259338 2316 66-67 -0.33032100555522703 2316 68-69 -0.2687780025677995 2316 70-71 -0.04281498666224337 2316 72-73 -0.19483072250428535 2316 74-75 -0.28149431351883436 2316 76-77 -0.41563648664920905 2316 78-79 -0.38775523473015383 2316 80-81 -0.4048755433123823 2316 82-83 -0.549930323040531 2316 84-85 -0.4008673672451515 2316 86-87 -0.717178556261775 2316 88-89 -0.5205920211365331 2316 90-91 -0.703012137713948 2316 92-93 -0.5155335275564887 2316 94-95 -0.45318663448257723 2316 96-97 -0.3189391995503783 2316 98-99 -0.21081787881421477 2316 100-101 -0.7504592905232244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 25.0 5 48.0 6 89.0 7 269.0 8 629.0 9 1154.0 10 2105.0 11 3808.0 12 5994.0 13 8789.0 14 10930.0 15 15582.0 16 22260.0 17 32938.0 18 28887.0 19 15303.0 20 11550.0 21 11049.0 22 11189.0 23 12163.0 24 13656.0 25 16063.0 26 19417.0 27 24332.0 28 30086.0 29 37503.0 30 46789.0 31 59606.0 32 76817.0 33 101677.0 34 140840.0 35 203905.0 36 316039.0 37 498205.0 38 446193.0 39 44846.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08482531428005 20.461774070931988 15.850291418309222 20.60310919647874 2 15.241809548722582 22.330556282186446 39.74252605081836 22.68510811827261 3 19.294340117863936 26.76124318178015 31.10618763635339 22.83822906400253 4 12.211670660934649 18.23839130086306 39.014441899034416 30.535496139167872 5 12.244606762454453 39.40756079365345 34.29652523391321 14.051307209978889 6 29.067789817831436 38.805920852721165 17.580812364527375 14.545476964920024 7 26.315796427089854 35.037745171868025 21.313801495010026 17.332656906032092 8 26.10397251309477 34.01398830520611 20.138332906162447 19.74370627553667 9 26.57685508866218 17.06173367965002 21.246951453129665 35.114459778558135 10-11 24.203341582441578 27.718809265923316 27.452817911963468 20.625031239671642 12-13 26.290487022352778 25.65370480063947 27.823868653482396 20.23193952352536 14-15 22.729190392266183 26.663148011629616 28.062991411663262 22.54467018444094 16-17 20.429117376871293 29.774008985561622 28.407062147639067 21.389811489928015 18-19 20.248340419085466 28.145034644880067 31.755927766724177 19.850697169310283 20-21 21.972349272532384 26.359438318725854 31.737233535644986 19.930978873096777 22-23 21.124988164625112 26.720555238722266 31.687713449609927 20.4667431470427 24-25 21.200621823722663 26.856068946686985 31.86762905484556 20.075680174744797 26-27 21.023305526062106 26.664439794975138 32.31636345540612 19.995891223556633 28-29 20.355191046259655 27.71703886940267 31.899968005369285 20.027802078968385 30-31 20.88157275013063 26.837274141103695 31.728855044933425 20.552298063832257 32-33 20.667518366146222 27.091772160260426 31.7027736161645 20.537935857428856 34-35 21.418062018166655 26.962431861839725 31.572068132435305 20.047437987558318 36-37 21.198778290994596 26.58947789797528 31.866492774314782 20.345251036715347 38-39 20.672906168563436 27.40927822225941 31.660697633708722 20.257117975468432 40-41 21.055971533665602 27.415603417809738 31.2701186060568 20.25830644246786 42-43 20.744734967951977 27.512123986841658 30.681955816760986 21.061185228445378 44-45 21.22456070662147 27.101806496446766 30.567799435340497 21.105833361591266 46-47 21.725558999345594 27.789778197021782 29.996358037902827 20.488304765729797 48-49 21.530892719090822 27.869141234396423 29.552194899182144 21.04777114733061 50-51 21.063532201198367 28.171215376038088 29.77224745613131 20.993004966632228 52-53 21.611059586375276 28.74065178350451 28.568836606695395 21.079452023424817 54-55 21.325273455424938 28.15373219644224 28.847180275802664 21.67381407233016 56-57 21.81022250758999 28.20389174697039 28.474374590994884 21.511511154444733 58-59 22.2661944006264 27.936183311798636 28.59997163937617 21.197650648198792 60-61 21.735093277387158 28.321539893797393 29.300113530571785 20.643253298243668 62-63 21.411412244567224 28.21355813971923 29.240529797591464 21.134499818122084 64-65 21.304448060963026 28.724452037616587 28.809203640994056 21.16189626042633 66-67 21.635060424863283 28.277633772746853 28.35734297946849 21.729962822921372 68-69 21.635897151342682 28.28322662868809 28.569651314056916 21.51122490591231 70-71 21.949515447291958 28.181520323205422 28.512335550218122 21.3566286792845 72-73 21.743020159823566 27.832671438649893 29.04973414117073 21.37457426035581 74-75 21.26844211217948 29.013160386373865 28.303506236254567 21.41489126519209 76-77 21.26458876655067 28.163794933312904 28.63947393685093 21.932142363285497 78-79 21.312612462644566 28.37757854879921 28.510992384027507 21.798816604528717 80-81 21.592326071500054 28.4486184872114 28.313421073665634 21.645634367622907 82-83 21.510331846890846 28.236997885276473 28.60286883693729 21.649801430895398 84-85 21.236983602052455 28.32891810890848 28.39308217161663 22.041016117422437 86-87 21.041072700961884 28.439848614160756 28.261890103463433 22.257188581413928 88-89 21.111511859056506 28.23456437817571 28.002438837496268 22.651484925271518 90-91 21.29419513474483 27.632891219581694 28.311443450640905 22.76147019503257 92-93 21.5013024646639 27.93673302667245 28.454922391602572 22.107042117061077 94-95 21.063444124726853 27.849361930002104 28.60270200999316 22.484491935277887 96-97 20.47899067886702 28.04909734828167 29.02681223945879 22.44509973339252 98-99 20.764914338912543 28.240194543567306 28.089165214503925 22.905725903016226 100-101 20.501678803038867 27.93082543112988 28.49030165603289 23.077194109798366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 842.0 1 587.0 2 396.0 3 495.5 4 472.5 5 502.0 6 853.0 7 1803.0 8 2985.5 9 3638.0 10 3533.5 11 3315.5 12 3570.5 13 3889.5 14 4183.5 15 4450.0 16 4661.5 17 5057.5 18 5308.0 19 5572.0 20 6262.0 21 7068.0 22 8006.5 23 9337.0 24 11119.5 25 13552.5 26 16815.5 27 20450.5 28 23506.0 29 27119.5 30 31801.5 31 36290.5 32 41419.0 33 47821.5 34 55690.5 35 61355.5 36 67813.5 37 74334.0 38 75928.0 39 76364.5 40 76886.0 41 77152.5 42 77170.0 43 79029.0 44 82381.5 45 83654.5 46 82661.5 47 91207.5 48 110102.0 49 104879.0 50 82228.5 51 69899.0 52 62606.5 53 57792.0 54 53426.5 55 48570.5 56 43304.5 57 38395.0 58 35138.5 59 31784.0 60 27561.5 61 22859.0 62 17949.5 63 12953.5 64 8636.0 65 6319.0 66 4671.0 67 3447.0 68 2501.0 69 1752.5 70 1300.0 71 1019.0 72 853.0 73 771.0 74 622.5 75 477.0 76 350.0 77 221.0 78 150.0 79 100.0 80 71.5 81 59.0 82 39.0 83 23.0 84 15.0 85 7.0 86 2.5 87 1.0 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07394019783737033 2 0.0 3 0.0 4 0.0 5 0.0017615294303125746 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.5413382515235027E-4 12-13 0.005240550055179909 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.367264793984729E-4 24-25 6.165353006094011E-4 26-27 2.2019117878907182E-4 28-29 0.002664313263347769 30-31 2.42210296667979E-4 32-33 0.0 34-35 0.0 36-37 2.2019117878907184E-5 38-39 2.8624853242579336E-4 40-41 6.605735363672154E-5 42-43 0.011229750118242662 44-45 2.2019117878907182E-4 46-47 0.0 48-49 2.2019117878907184E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.2019117878907184E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2019117878907184E-5 82-83 3.5230588606251494E-4 84-85 5.504779469726796E-4 86-87 0.0 88-89 0.0 90-91 0.00812505449731675 92-93 0.0025982559097110476 94-95 0.0 96-97 0.0 98-99 1.321147072734431E-4 100-101 0.010459080992480912 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2270754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.501867417718415 #Duplication Level Percentage of deduplicated Percentage of total 1 68.15864200692947 30.331868499641086 2 15.592672875370997 13.878061219752286 3 6.379906685634816 8.517532843846078 4 3.1914093542476123 5.680947038335743 5 1.8842171110533341 4.192559003114595 6 1.1457126861901288 3.059181243577868 7 0.7858745188030394 2.448101854991528 8 0.5291930754024785 1.884006406394861 9 0.3745985242491828 1.5003300474909098 >10 1.7698963014012894 13.713449003542777 >50 0.11582311417524946 3.5649240842775294 >100 0.06621781337353379 5.219879333925393 >500 0.002670681280709755 0.7506497180375645 >1k 0.002571767159201884 2.353676210840747 >5k 2.9674236452329427E-4 0.9300696588527052 >10k+ 2.9674236452329427E-4 1.9747638333782982 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16746 0.7374642960003593 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14596 0.6427820891210584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13520 0.5953969474456502 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9294 0.4092913631331267 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6031 0.2655945998553784 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5804 0.25559792033835455 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4117 0.18130541661492172 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4064 0.17897139011975757 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3972 0.17491987243003865 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3402 0.14981807804808447 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3349 0.14748405155292033 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3139 0.13823602204377927 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3043 0.13400835141102913 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2993 0.1318064396231384 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2478 0.109126748207864 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2281 0.10045121576357458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.403823575781437E-5 0.0 8 0.0 0.0 0.0 4.403823575781437E-5 0.0 9 0.0 0.0 0.0 4.403823575781437E-5 0.0 10-11 0.0 0.0 0.0 6.605735363672156E-5 0.0 12-13 0.0 0.0 0.0 8.807647151562874E-5 0.0 14-15 0.0 0.0 0.0 1.1009558939453591E-4 0.0 16-17 0.0 0.0 0.0 1.7615294303125747E-4 0.0 18-19 0.0 0.0 0.0 1.7615294303125747E-4 0.0 20-21 0.0 0.0 0.0 1.7615294303125747E-4 0.0 22-23 0.0 0.0 0.0 2.42210296667979E-4 0.0 24-25 0.0 0.0 0.0 4.4038235757814365E-4 0.0 26-27 0.0 0.0 0.0 8.147073615195657E-4 0.0 28-29 0.0 0.0 0.0 0.00257623679183214 0.0 30-31 0.0 0.0 0.0 0.005813047120031496 0.0 32-33 0.0 0.0 0.0 0.01023888981369184 0.0 34-35 0.0 0.0 0.0 0.016029917815844428 0.0 36-37 0.0 0.0 0.0 0.023714589955583038 0.0 38-39 0.0 0.0 0.0 0.035781066553224175 0.0 40-41 0.0 0.0 0.0 0.048353982862080175 0.0 42-43 0.0 0.0 0.0 0.060156230045174425 0.0 44-45 0.0 0.0 0.0 0.06880974337158495 0.0 46-47 0.0 0.0 0.0 0.083980915590152 0.0 48-49 0.0 0.0 0.0 0.09703825249234396 0.0 50-51 0.0 0.0 0.0 0.11187913794272739 0.0 52-53 0.0 0.0 0.0 0.14541425447230305 0.0 54-55 0.0 0.0 0.0 0.19621235941894188 0.0 56-57 0.0 4.403823575781437E-5 0.0 0.27206822051177715 0.0 58-59 0.0 8.807647151562874E-5 0.0 0.36983310389412505 0.0 60-61 0.0 8.807647151562874E-5 0.0 0.4812498403613954 0.0 62-63 0.0 8.807647151562874E-5 0.0 0.5556524396742227 0.0 64-65 0.0 8.807647151562874E-5 0.0 0.6503566656714026 0.0 66-67 0.0 8.807647151562874E-5 0.0 0.8119109335489445 0.0 68-69 0.0 8.807647151562874E-5 0.0 1.013209709197914 0.0 70-71 0.0 8.807647151562874E-5 0.0 1.229327351179388 0.0 72-73 0.0 8.807647151562874E-5 0.0 1.4799929891128674 0.0 74-75 0.0 8.807647151562874E-5 0.0 1.7477234434024997 0.0 76-77 0.0 8.807647151562874E-5 0.0 1.9927301680411 0.0 78-79 0.0 8.807647151562874E-5 0.0 2.2415462000727513 0.0 80-81 0.0 8.807647151562874E-5 0.0 2.5033314925350787 2.2019117878907184E-5 82-83 0.0 8.807647151562874E-5 0.0 2.842381869634491 4.403823575781437E-5 84-85 0.0 8.807647151562874E-5 0.0 3.317334242282519 4.403823575781437E-5 86-87 0.0 8.807647151562874E-5 0.0 3.873757351082504 4.403823575781437E-5 88-89 0.0 8.807647151562874E-5 0.0 4.513060419578695 4.403823575781437E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14220 0.0 23.099194 1 GAGTACT 14130 0.0 23.045042 12-13 TACATGG 14260 0.0 22.349972 2 TGTACCG 150 3.035955E-5 22.165663 5 AGTACTT 14640 0.0 21.83666 12-13 GTACTTT 15010 0.0 21.486372 14-15 ACATGGG 14285 0.0 21.0806 3 AGAGTAC 23210 0.0 20.507126 10-11 GTATCAA 33110 0.0 19.381748 1 TACTTTT 16660 0.0 19.130287 14-15 TACGACG 175 9.945652E-5 18.999142 5 ACTTTTT 17080 0.0 18.951866 16-17 CATGGGG 4740 0.0 18.437984 4 ACCGTCG 130 0.0075634713 18.270016 8 CATGGGA 8695 0.0 17.862907 4 CGACGGT 160 0.0011911583 17.813263 7 ATGGGAT 3840 0.0 16.945848 5 CAACGCA 37700 0.0 16.932922 5 TCAACGC 37765 0.0 16.916355 4 ATCAACG 37855 0.0 16.876135 3 >>END_MODULE