FastQCFastQC Report
Thu 26 May 2016
SRR937423_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937423_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825661
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149320.8178955457776662No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104740.5737100151671093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77650.4253254026897655No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27650.15145199464741813No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.1348004914384434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72350.045.3855741
GTATCAA124650.039.190351
GCGTATC702.0486768E-433.9430051
TATCAAC160750.030.350552
ATCAACG160900.030.1442783
TCAACGC162250.029.8105374
CAACGCA164600.029.3288125
AACGCAG168800.028.6201386
GTACATG118800.028.5601221
TACATGG120500.027.596762
ACATGGG121000.025.990093
ACGCAGA187850.025.4662727
CGCAGAG187800.025.4224598
GCAGAGT192850.024.190179
GTGCGAA1201.7099228E-423.7529269
GAGTACT109850.023.13476212-13
AGTACTT111950.022.9553812-13
CATGGGG42700.022.8103894
CATGGGA79850.022.7892784
GTACTTT116600.021.7939914-15
Produced by FastQC (version 0.11.5)