##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937423_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1825661 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.765768124531334 33.0 31.0 34.0 30.0 34.0 2 31.858114403495502 33.0 31.0 34.0 30.0 34.0 3 31.87440439380586 33.0 31.0 34.0 30.0 34.0 4 35.526614196173334 37.0 35.0 37.0 33.0 37.0 5 35.54167723361566 37.0 35.0 37.0 33.0 37.0 6 35.59524413349466 37.0 35.0 37.0 33.0 37.0 7 35.531990331173205 37.0 35.0 37.0 33.0 37.0 8 35.51744272348481 37.0 35.0 37.0 33.0 37.0 9 37.11235273142166 39.0 37.0 39.0 33.0 39.0 10-11 36.997093929267265 39.0 37.0 39.0 33.0 39.0 12-13 36.84121531872566 39.0 37.0 39.0 33.0 39.0 14-15 37.90355328837062 40.0 38.0 41.0 33.0 41.0 16-17 37.83732302985056 40.0 38.0 41.0 33.0 41.0 18-19 37.81677923776648 40.0 38.0 41.0 33.0 41.0 20-21 37.71411039617979 40.0 37.0 41.0 32.5 41.0 22-23 37.77423985066231 40.0 37.5 41.0 33.0 41.0 24-25 37.72734149439573 40.0 37.0 41.0 32.5 41.0 26-27 37.63664174236071 40.0 37.0 41.0 32.0 41.0 28-29 37.483618809844764 40.0 37.0 41.0 32.0 41.0 30-31 37.1756569264502 39.0 36.5 41.0 31.5 41.0 32-33 36.874363038921246 39.0 35.5 41.0 31.0 41.0 34-35 36.82150328018181 39.0 36.0 41.0 31.0 41.0 36-37 36.65485569336257 39.0 35.0 41.0 30.5 41.0 38-39 36.48417668997695 39.0 35.0 41.0 30.0 41.0 40-41 36.20821171071738 39.0 35.0 40.5 30.0 41.0 42-43 35.87355319525366 39.0 35.0 40.0 29.0 41.0 44-45 35.51593450262672 39.0 35.0 40.0 27.5 41.0 46-47 34.96896849962835 38.0 34.5 40.0 25.0 41.0 48-49 34.50415329023296 38.0 34.0 40.0 23.5 41.0 50-51 33.49622629831059 37.5 33.0 39.5 21.0 40.5 52-53 33.576673599315534 37.5 33.0 39.5 21.5 40.0 54-55 33.85264569928371 38.0 34.0 40.0 20.5 41.0 56-57 33.57131882644149 38.0 33.0 40.0 18.5 41.0 58-59 33.281885300721214 37.5 33.0 40.0 17.0 41.0 60-61 32.93150891649655 37.0 32.5 40.0 13.5 41.0 62-63 32.53232445673101 36.5 32.0 39.5 9.0 41.0 64-65 32.0881143870631 36.0 31.0 39.0 7.5 41.0 66-67 31.64117790761812 35.5 31.0 39.0 4.5 40.0 68-69 31.13706049480161 35.0 30.5 38.5 2.0 40.0 70-71 30.65592708613483 35.0 30.0 38.0 2.0 40.0 72-73 30.15797894570788 35.0 29.0 37.0 2.0 39.5 74-75 29.600245061925516 34.0 29.0 36.5 2.0 39.0 76-77 29.068585569829228 34.0 28.0 36.0 2.0 39.0 78-79 28.514307420709542 34.0 27.5 35.5 2.0 38.0 80-81 27.91776047141282 33.5 26.0 35.0 2.0 37.0 82-83 27.34929951398425 33.0 26.0 35.0 2.0 36.5 84-85 26.754674334391765 33.0 25.0 35.0 2.0 36.0 86-87 26.133571621456557 32.0 23.5 35.0 2.0 36.0 88-89 25.542759581324244 32.0 20.5 35.0 2.0 35.0 90-91 24.953463156632036 31.5 18.5 34.0 2.0 35.0 92-93 24.247039839269174 31.0 11.5 34.0 2.0 35.0 94-95 23.435526913265935 31.0 2.0 34.0 2.0 35.0 96-97 22.52956764700566 30.5 2.0 34.0 2.0 35.0 98-99 21.321756065337432 29.5 2.0 34.0 2.0 35.0 100-101 18.2894072338731 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1679786963484169 1101 2 0.2379145772538216 1101 3 0.12136242482995385 1101 4 0.16424945502906496 1101 5 0.08814674328851879 1101 6 0.16321241618918236 1101 7 0.23613867767114272 1101 8 0.13743779968663716 1101 9 0.22014256751202765 1101 10-11 0.24217059350628745 1101 12-13 0.20614848423583965 1101 14-15 0.4408600196170269 1101 16-17 0.4021947878945511 1101 18-19 0.3489306587807164 1101 20-21 0.456622772350606 1101 22-23 0.38057910043391985 1101 24-25 0.3743411702391981 1101 26-27 0.45146905104947876 1101 28-29 0.3390681344691586 1101 30-31 0.3615430654991698 1101 32-33 0.4352755193076163 1101 34-35 0.6020459412478161 1101 36-37 0.5555257530931499 1101 38-39 0.695838739308904 1101 40-41 0.7218260794377684 1101 42-43 0.6165387630225823 1101 44-45 0.659359662430802 1101 46-47 0.8299914610433277 1101 48-49 0.876011193355609 1101 50-51 0.7991755038521262 1101 52-53 0.8780241273471603 1101 54-55 0.938615027023026 1101 56-57 0.9864293020991397 1101 58-59 0.9246966846656335 1101 60-61 1.0400249086573368 1101 62-63 1.0432704214473603 1101 64-65 0.9542893267228614 1101 66-67 1.1750623612231017 1101 68-69 1.2685685366440502 1101 70-71 1.059519586201084 1101 72-73 1.0833338195428759 1101 74-75 1.01710786990337 1101 76-77 1.123462772003478 1101 78-79 1.3121197489964267 1101 80-81 1.2899246339842279 1101 82-83 1.2583135439117257 1101 84-85 1.4169008550180173 1101 86-87 1.3262555944141177 1101 88-89 1.5196175288707785 1101 90-91 1.5075773809748227 1101 92-93 1.9169278387668847 1101 94-95 1.856708585603446 1101 96-97 1.728616085604962 1101 98-99 1.9629720734599196 1101 100-101 2.2237201304455247 1102 1 0.07191864148576244 1102 2 0.10543554982669434 1102 3 0.0642166271160356 1102 4 0.10202403085168754 1102 5 0.022526219782854184 1102 6 0.040072642445124984 1102 7 -0.12109743758244207 1102 8 -0.05931749019065791 1102 9 0.09813911444144452 1102 10-11 0.08394833674402946 1102 12-13 0.1303283799715942 1102 14-15 0.2815772669064458 1102 16-17 0.38002414235518955 1102 18-19 0.3653821036513065 1102 20-21 0.23865567313994518 1102 22-23 0.20525742296415217 1102 24-25 0.18319712006465494 1102 26-27 0.29843270139037514 1102 28-29 0.24427735609999957 1102 30-31 0.4420183037904408 1102 32-33 0.43132465204919157 1102 34-35 0.4899320487298908 1102 36-37 0.6189465194059736 1102 38-39 0.31817867772494424 1102 40-41 0.30280079472748156 1102 42-43 0.4838377224805086 1102 44-45 0.6036289095185765 1102 46-47 0.6380137589735781 1102 48-49 0.5275222267983111 1102 50-51 0.44840423407153907 1102 52-53 0.3850760368279751 1102 54-55 0.5248462231213082 1102 56-57 0.5500586389163544 1102 58-59 0.5752112846009396 1102 60-61 0.5443748534166062 1102 62-63 0.5635943883617358 1102 64-65 0.45090283906695916 1102 66-67 0.6946331218648218 1102 68-69 0.8154803069098371 1102 70-71 0.8740415963754593 1102 72-73 0.8010881474333544 1102 74-75 0.7047179837874857 1102 76-77 0.8493513495652429 1102 78-79 0.977669698796678 1102 80-81 1.154624758748458 1102 82-83 1.0885787414130803 1102 84-85 1.2303325434519117 1102 86-87 1.2497276526878345 1102 88-89 1.3049953292396133 1102 90-91 1.497472948694078 1102 92-93 1.4151093056077428 1102 94-95 1.5579871038916977 1102 96-97 1.405846823814322 1102 98-99 1.4505437498794755 1102 100-101 1.344865776930071 1103 1 0.2800156813662973 1103 2 0.271975480748079 1103 3 0.31886156852747405 1103 4 0.23438470551339918 1103 5 0.15660744670702087 1103 6 0.2262752425129193 1103 7 0.23711549679287458 1103 8 0.29054966631730395 1103 9 0.45622516037780514 1103 10-11 0.30311123951175034 1103 12-13 0.29191737136883233 1103 14-15 0.3666981086761041 1103 16-17 0.43131020478246285 1103 18-19 0.42137792595252677 1103 20-21 0.28645780046814195 1103 22-23 0.3018733495246053 1103 24-25 0.42728535562823566 1103 26-27 0.43094271046037846 1103 28-29 0.2976153923983418 1103 30-31 0.27853881207712305 1103 32-33 0.460988832862931 1103 34-35 0.66818534004193 1103 36-37 0.5138673171998533 1103 38-39 0.3920559211766772 1103 40-41 0.5331074341535995 1103 42-43 0.425850622041672 1103 44-45 0.5842415470574025 1103 46-47 0.3172828449187435 1103 48-49 0.41707248286559206 1103 50-51 0.36617754810438896 1103 52-53 0.15360151987440673 1103 54-55 0.28555730226854337 1103 56-57 0.26674263631444717 1103 58-59 0.5780523030066433 1103 60-61 0.4729268135452358 1103 62-63 0.5868578032640244 1103 64-65 0.41948855976581356 1103 66-67 0.7997574450501297 1103 68-69 0.80338742640485 1103 70-71 0.8419566864799926 1103 72-73 0.9231261956417995 1103 74-75 0.792346819070719 1103 76-77 0.8422217767715914 1103 78-79 0.8556563869626146 1103 80-81 1.1231889496399745 1103 82-83 1.2696815256294371 1103 84-85 1.3038440092195138 1103 86-87 1.4221811803696731 1103 88-89 1.5485503585109903 1103 90-91 1.4801245047952563 1103 92-93 1.5386100064716821 1103 94-95 1.767078408598021 1103 96-97 2.061607879599425 1103 98-99 2.1998126525922324 1103 100-101 2.098829093803026 1104 1 0.1712300579245003 1104 2 0.17317429249652605 1104 3 0.11110097274879749 1104 4 0.22859037220607803 1104 5 0.1956044117986906 1104 6 0.19577234764196305 1104 7 0.2409910885081743 1104 8 0.2035692394099229 1104 9 0.3539824962700777 1104 10-11 0.3395064938517649 1104 12-13 0.40548722247007163 1104 14-15 0.5382683408626647 1104 16-17 0.4446466985221065 1104 18-19 0.5405547816773009 1104 20-21 0.44657331982314474 1104 22-23 0.4910584467931898 1104 24-25 0.4838447291790757 1104 26-27 0.5247739493549588 1104 28-29 0.5784314200201379 1104 30-31 0.49695120148204097 1104 32-33 0.38689930536280315 1104 34-35 0.4461697430924474 1104 36-37 0.6177768851960366 1104 38-39 0.6808243498655173 1104 40-41 0.7728314457422627 1104 42-43 0.8140103472929354 1104 44-45 0.8239740527162951 1104 46-47 0.7232140016274471 1104 48-49 0.7257049834739462 1104 50-51 0.5600196915485682 1104 52-53 0.6147596289566266 1104 54-55 0.806321516061999 1104 56-57 0.788684771034994 1104 58-59 0.8852313993293919 1104 60-61 0.8643655942558937 1104 62-63 0.7333009687514647 1104 64-65 0.6800473777585445 1104 66-67 0.9994963097832148 1104 68-69 1.021092741856247 1104 70-71 0.9680332524881017 1104 72-73 0.9944439014163926 1104 74-75 1.062802721072103 1104 76-77 1.0116011127648683 1104 78-79 1.062197854095153 1104 80-81 0.9016948197312047 1104 82-83 1.1542448156353622 1104 84-85 1.1995655230923994 1104 86-87 1.1374198866998206 1104 88-89 1.1182284791635162 1104 90-91 1.0173854655893848 1104 92-93 1.1695175175254526 1104 94-95 1.203147329942933 1104 96-97 1.2425716351722222 1104 98-99 1.4798963536193277 1104 100-101 1.5349536857433037 1105 1 0.13444313960536647 1105 2 0.15157146804270383 1105 3 0.23409956583100922 1105 4 0.21879822440007501 1105 5 0.16426677807513812 1105 6 0.15221523474344423 1105 7 0.245375362883685 1105 8 0.2348665237931371 1105 9 0.275196161954554 1105 10-11 0.3523237803586241 1105 12-13 0.3842507275535567 1105 14-15 0.3619284507615248 1105 16-17 0.40995157495225243 1105 18-19 0.515382633340387 1105 20-21 0.4716939696598885 1105 22-23 0.4888164038423781 1105 24-25 0.41358697122202415 1105 26-27 0.46577578100264105 1105 28-29 0.40706193155421033 1105 30-31 0.3613609596514493 1105 32-33 0.39577562932553434 1105 34-35 0.5429184357207291 1105 36-37 0.5897659484011513 1105 38-39 0.640188847755141 1105 40-41 0.7751610892346079 1105 42-43 0.5687463511155002 1105 44-45 0.4978600658298262 1105 46-47 0.7996420231824928 1105 48-49 0.8736989310728944 1105 50-51 0.8761525258242742 1105 52-53 0.7955289209849781 1105 54-55 1.041676563711384 1105 56-57 1.0886215924626157 1105 58-59 1.197880917526419 1105 60-61 1.1849357941444012 1105 62-63 1.1365071485714964 1105 64-65 1.1653405741164882 1105 66-67 1.295079649752953 1105 68-69 1.2694339061470892 1105 70-71 1.426688982359888 1105 72-73 1.424253569330702 1105 74-75 1.4225324866742888 1105 76-77 1.256583194472281 1105 78-79 1.4257140867409568 1105 80-81 1.6417941003449386 1105 82-83 1.5896513617849202 1105 84-85 1.6037167269362023 1105 86-87 1.6858868772230338 1105 88-89 1.7313632512693822 1105 90-91 1.9277669261293227 1105 92-93 2.047388027997698 1105 94-95 2.0190115225579426 1105 96-97 1.898026494347718 1105 98-99 2.0252341466165937 1105 100-101 1.5020886171228796 1106 1 0.13949681111671808 1106 2 -0.5499579325462598 1106 3 -0.028440569060222742 1106 4 0.23213155773341043 1106 5 0.14247899428108468 1106 6 0.0022655814709082733 1106 7 0.135893079232126 1106 8 0.13882776631237448 1106 9 0.11740381845687864 1106 10-11 -0.1881606881509157 1106 12-13 -0.08176998110037204 1106 14-15 0.057758221826397005 1106 16-17 0.11305787304287662 1106 18-19 0.26685218781933884 1106 20-21 0.2160997832820044 1106 22-23 0.31925432537747866 1106 24-25 0.21573003192307993 1106 26-27 0.2695318368590094 1106 28-29 0.3729230505814698 1106 30-31 0.45414522867396556 1106 32-33 0.559235304446652 1106 34-35 0.4872197561349054 1106 36-37 0.495095102006168 1106 38-39 0.5771756436134083 1106 40-41 0.7079035609739392 1106 42-43 0.8400278178201646 1106 44-45 0.7490028415494052 1106 46-47 0.9760607559648591 1106 48-49 0.9210001564932497 1106 50-51 0.767671477092442 1106 52-53 0.8811164577950876 1106 54-55 0.9953556745691756 1106 56-57 0.8414723571629139 1106 58-59 0.7657027091099593 1106 60-61 0.871579662292973 1106 62-63 1.0957965947574877 1106 64-65 1.0745682743159755 1106 66-67 1.2004430011351097 1106 68-69 1.2905301348922187 1106 70-71 1.277031910069585 1106 72-73 1.2028400612087289 1106 74-75 1.1719330756360087 1106 76-77 1.1307410742100963 1106 78-79 1.3554908512512647 1106 80-81 1.3495968931634081 1106 82-83 1.3573724599135417 1106 84-85 1.058237483087055 1106 86-87 1.0787148434052334 1106 88-89 1.3642976105360276 1106 90-91 1.0770060255878562 1106 92-93 1.3062158041922451 1106 94-95 1.4785403531839485 1106 96-97 1.201524166998997 1106 98-99 1.2987175701940608 1106 100-101 0.9120078723712908 1107 1 0.0032448566829472725 1107 2 0.10969510892496359 1107 3 -0.03424802941313487 1107 4 0.015751541353395737 1107 5 0.00792460518123761 1107 6 -0.08699061687620002 1107 7 0.008084278696053104 1107 8 0.00851729054735273 1107 9 0.06439212908155412 1107 10-11 0.034391616219565435 1107 12-13 -0.013941822363129575 1107 14-15 0.11370342322614846 1107 16-17 0.09108631470768103 1107 18-19 0.04951915230449089 1107 20-21 0.08918443818483723 1107 22-23 0.18486373644143583 1107 24-25 0.18116075189925596 1107 26-27 0.19340943346387718 1107 28-29 0.04868807180103829 1107 30-31 0.011110458366466958 1107 32-33 0.12720492696172414 1107 34-35 0.2972413479746763 1107 36-37 0.2713440782551402 1107 38-39 0.3675412157971607 1107 40-41 0.2672438930415453 1107 42-43 0.2426784022889379 1107 44-45 0.3003244110527916 1107 46-47 0.44409738763785356 1107 48-49 0.5377301095868319 1107 50-51 0.46977109737388645 1107 52-53 0.5755695886118488 1107 54-55 0.6364620702210289 1107 56-57 0.6509876575873079 1107 58-59 0.7114401821201639 1107 60-61 0.7842071658295637 1107 62-63 0.7772052761661712 1107 64-65 0.5868458411389987 1107 66-67 0.8760963404248123 1107 68-69 1.0061804959971248 1107 70-71 0.9547699787167439 1107 72-73 1.01146190619421 1107 74-75 0.9808080663291818 1107 76-77 1.0300933190168884 1107 78-79 1.1359465135251092 1107 80-81 1.1307822107123613 1107 82-83 1.0834241314197612 1107 84-85 0.8305850372527921 1107 86-87 0.8777618242703973 1107 88-89 0.8376949748424813 1107 90-91 0.7989403566130981 1107 92-93 0.9799407688263031 1107 94-95 1.1227738428719825 1107 96-97 1.407197609036075 1107 98-99 1.6502029398612486 1107 100-101 1.230904454904234 1108 1 0.18988664426813173 1108 2 0.3201125344367277 1108 3 0.22057259616220648 1108 4 0.31955294138121104 1108 5 0.2357337669526629 1108 6 0.22803021135217705 1108 7 0.31397777326878895 1108 8 0.19603856580447854 1108 9 0.3148550521859903 1108 10-11 0.34970566219399046 1108 12-13 0.3900536212549568 1108 14-15 0.48508348375715116 1108 16-17 0.5518939797606279 1108 18-19 0.5191839469692852 1108 20-21 0.376352395164119 1108 22-23 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0.4334464766642512 2316 98-99 1.0814498849012963 2316 100-101 1.8448472378125338 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6502.0 3 1266.0 4 1149.0 5 1343.0 6 1596.0 7 2004.0 8 2447.0 9 3246.0 10 4165.0 11 5780.0 12 8228.0 13 11073.0 14 12555.0 15 16453.0 16 21763.0 17 29064.0 18 24198.0 19 15547.0 20 15245.0 21 16045.0 22 17187.0 23 19314.0 24 21782.0 25 24731.0 26 28736.0 27 33245.0 28 39680.0 29 46131.0 30 55944.0 31 68861.0 32 85075.0 33 108789.0 34 141917.0 35 190022.0 36 249288.0 37 293605.0 38 184882.0 39 16801.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4608847096197 18.75354596198344 15.240015971589463 21.545553356807396 2 14.958338640745994 20.401657669687133 41.351369751700936 23.28863393786594 3 18.345525982311692 26.192654102857492 30.678037199475543 24.783782715355272 4 11.94692063241072 16.766702464328617 38.193854222257364 33.0925226810033 5 12.769158294561214 38.0571078434064 34.046568269491566 15.12716559254082 6 30.119424465960158 36.88073636213437 18.08426843251889 14.91557073938658 7 27.419150327120274 34.3149960451354 20.821525002374017 17.444328625370307 8 26.724379738177852 33.78640893943311 20.07535524497865 19.413856077410387 9 27.49998627572615 16.047628719964425 20.46108070421994 35.99130430008948 10-11 24.53872639485597 27.097180462943975 27.247747195025507 21.116345947174548 12-13 26.102332659372223 24.73386953774466 28.13338391244032 21.0304138904428 14-15 22.82814127065421 25.323745706674394 27.53966986199329 24.308443160678106 16-17 21.051165438182746 29.064227062361354 27.823438633392716 22.061168866063188 18-19 20.96905871547196 26.530537199052368 31.8124626272389 20.68794145823677 20-21 22.103975897728915 25.526331533566825 31.83251248831579 20.537180080388474 22-23 21.67125924450105 25.619751021083598 31.776714271953605 20.932275462461746 24-25 21.273361472301048 25.828059563864297 31.962199010928096 20.936379952906563 26-27 21.03727820604894 26.059947107269277 31.78923369207122 21.11354099461056 28-29 20.778059883736727 26.123200865511205 32.116240218880804 20.982499031871267 30-31 20.961087996802853 25.99308418920929 32.00868126939548 21.037146544592378 32-33 20.833539176648692 26.096788075798642 31.785336710261536 21.284336037291123 34-35 21.21426530641931 26.192345767990748 31.481496220859146 21.111892704730796 36-37 21.343552327060493 25.939272787250022 31.442534077720445 21.274640807969035 38-39 21.079310579500476 26.120138173883074 31.532799283445257 21.267751963171193 40-41 21.155732713828197 26.179799731024566 31.47531185865536 21.189155696491877 42-43 21.082407867087433 26.29902144414174 31.09964518190842 21.518925506862406 44-45 21.13385073080266 26.503242172696158 30.799529199587155 21.56337789691403 46-47 21.34387536189166 26.785183526310195 30.15071763572258 21.72022347607556 48-49 21.54071755643064 26.96155547773239 29.671055015001695 21.826671950835276 50-51 21.624580048747283 27.194341362728093 29.39483103137832 21.786247557146307 52-53 21.776235063534024 27.331131808736377 29.22592528541586 21.66670784231374 54-55 21.31631621603893 27.257614626626196 29.33992617633312 22.086142981001757 56-57 21.535933148855317 27.58874757282619 29.00320403407538 21.872115244243112 58-59 21.68004421010115 27.079208301137957 29.25478320806406 21.985964280696823 60-61 21.66239282818346 27.010796415738152 29.659176816175552 21.667633939902835 62-63 21.630993928344257 27.101685827077183 29.603341907815988 21.663978336762575 64-65 21.377289099879786 27.188383463794086 29.598964545791052 21.83536289053507 66-67 21.352149794086912 27.46302674674886 29.212601968687164 21.972221490477068 68-69 21.342848996923085 27.395501445158477 29.44410585809869 21.817543699819744 70-71 21.311300864923574 27.4631826410478 29.216629341871613 22.008887152157016 72-73 21.489227354640704 27.41128034398702 29.03248025364899 22.067012047723285 74-75 21.29415506491247 27.618884535686973 28.947731665304175 22.139228734096385 76-77 21.428563587584144 27.430666854013992 28.976227381103975 22.164542177297896 78-79 21.391496598732804 27.453236178055317 28.9575494080475 22.19771781516438 80-81 21.30824307350926 27.768942900101727 28.730573979210305 22.192240047178704 82-83 21.332579979578224 27.570469813391274 28.80527113463253 22.291679072397976 84-85 21.378785983027555 27.300866841395905 28.76872412536603 22.55162305021051 86-87 21.427544435670146 27.267057430932574 28.68234772458747 22.62305040880981 88-89 21.458995574327588 27.022012012539403 28.769779418867063 22.749212994265942 90-91 21.250293269013092 27.076817959176953 29.10685693823034 22.56603183357961 92-93 21.339037632734044 26.750456568062603 29.19606974137186 22.714436057831495 94-95 21.080249852438005 26.761146590323172 29.185608705924516 22.97299485131431 96-97 21.136744213616627 26.64041519369174 29.07824014691604 23.144600445775588 98-99 21.018640181165743 26.716294049653726 28.97655734591746 23.288508423263067 100-101 21.361241603371823 25.992722922246124 29.71440488211793 22.931630592264128 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2922.0 1 1751.5 2 637.0 3 698.5 4 641.0 5 659.0 6 1081.0 7 2358.0 8 3990.5 9 4669.5 10 4206.0 11 3706.5 12 3688.5 13 3992.0 14 4391.0 15 4531.0 16 4647.0 17 4915.5 18 5089.5 19 5321.5 20 5835.5 21 6419.0 22 7231.5 23 8529.0 24 10005.0 25 11967.0 26 14663.5 27 17427.5 28 19760.5 29 22420.0 30 25710.0 31 28705.5 32 31508.5 33 34306.5 34 38442.5 35 41657.5 36 44272.5 37 48207.5 38 50795.5 39 51251.5 40 51949.0 41 54913.5 42 57805.5 43 60158.5 44 62163.5 45 63694.5 46 63225.5 47 63525.5 48 67616.5 49 67725.0 50 63467.5 51 60019.0 52 57633.5 53 56440.5 54 54861.5 55 50797.0 56 45309.0 57 40316.0 58 36685.0 59 33176.5 60 28998.5 61 24512.0 62 20032.5 63 15493.5 64 11302.0 65 8544.5 66 6366.5 67 4790.5 68 3502.5 69 2325.5 70 1677.5 71 1246.0 72 980.5 73 790.0 74 581.5 75 446.5 76 332.0 77 225.5 78 154.5 79 110.5 80 65.5 81 37.0 82 24.5 83 17.0 84 11.5 85 10.5 86 9.0 87 8.0 88 6.5 89 5.5 90 4.0 91 3.0 92 4.5 93 3.5 94 2.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2699296309665376 2 0.236024103050895 3 0.2379959915887999 4 0.23624320177732888 5 0.2374482447727152 6 0.21033477737652279 7 0.2111016229190414 8 0.21307351145694628 9 0.22298772882807924 10-11 0.23057402223085227 12-13 0.2241379971418571 14-15 0.21570269617415283 16-17 0.21008829130928472 18-19 0.19968110180367551 20-21 0.20584325348462829 22-23 0.20263893461053284 24-25 0.20545983071336904 26-27 0.2011052435254957 28-29 0.21074558748858632 30-31 0.22118016433499976 32-33 0.21290918741212086 34-35 0.21277225070809969 36-37 0.205925415507041 38-39 0.19768182592496633 40-41 0.19508002854856404 42-43 0.20493947123808856 44-45 0.20033839798297712 46-47 0.20871892426907296 48-49 0.21181369377995146 50-51 0.2202489947476558 52-53 0.22983456402913793 54-55 0.2129365747529251 56-57 0.19625768420314615 58-59 0.18913697559404513 60-61 0.19327246405548454 62-63 0.19653155761118848 64-65 0.18982165911415097 66-67 0.1935189501227227 68-69 0.19403930959800314 70-71 0.1967780436784266 72-73 0.1980378613554214 74-75 0.19885948157954844 76-77 0.20447388644441655 78-79 0.2063088382783003 80-81 0.20006452457493476 82-83 0.19644939558877578 84-85 0.18694598832970633 86-87 0.18732941110096563 88-89 0.1952717399341937 90-91 0.19327246405548454 92-93 0.19957155244045857 94-95 0.19420363364282855 96-97 0.20091353213986607 98-99 0.20173515236399311 100-101 0.19176616031125165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1825661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.1179925709987 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03578474566547 35.87371132554657 2 13.965697783769071 13.719340799839646 3 5.198105480797613 7.659615191672545 4 2.4772670162469757 4.867135316015963 5 1.3901554803683898 3.4140823278633854 6 0.8778626571799357 2.5871310884232757 7 0.5789411520655879 1.9905499044342085 8 0.4076119741112976 1.6016865532999067 9 0.3028632580884086 1.3388431734733424 >10 1.58073972564531 14.243091621938822 >50 0.12097341945557308 4.082388325245216 >100 0.06054138870424806 5.388401193769251 >500 0.002564068120887931 0.9081504616422665 >1k 5.574061132365066E-4 0.5095170362123177 >5k 1.1148122264730135E-4 0.4251907346790594 >10k+ 2.229624452946027E-4 1.3911649459441318 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14932 0.8178955457776662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10474 0.5737100151671093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7765 0.4253254026897655 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2765 0.15145199464741813 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2461 0.1348004914384434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 5.477468160846948E-5 0.0 0.0 0.0 3 0.0 5.477468160846948E-5 0.0 0.0 0.0 4 0.0 5.477468160846948E-5 0.0 0.0 0.0 5 0.0 5.477468160846948E-5 0.0 0.0 0.0 6 0.0 5.477468160846948E-5 0.0 0.0 0.0 7 0.0 5.477468160846948E-5 0.0 0.0 0.0 8 0.0 5.477468160846948E-5 0.0 0.0 0.0 9 0.0 5.477468160846948E-5 0.0 0.0 0.0 10-11 0.0 5.477468160846948E-5 0.0 0.0 0.0 12-13 0.0 5.477468160846948E-5 0.0 0.0 0.0 14-15 0.0 5.477468160846948E-5 0.0 0.0 0.0 16-17 0.0 5.477468160846948E-5 0.0 0.0 0.0 18-19 0.0 5.477468160846948E-5 0.0 5.477468160846948E-5 0.0 20-21 0.0 5.477468160846948E-5 0.0 5.477468160846948E-5 0.0 22-23 0.0 5.477468160846948E-5 0.0 1.369367040211737E-4 0.0 24-25 0.0 5.477468160846948E-5 0.0 3.286480896508169E-4 0.0 26-27 0.0 5.477468160846948E-5 0.0 6.846835201058685E-4 0.0 28-29 0.0 5.477468160846948E-5 0.0 0.0018349518338837277 0.0 30-31 0.0 5.477468160846948E-5 0.0 0.005450080820042714 0.0 32-33 0.0 5.477468160846948E-5 0.0 0.009448632577460985 0.0 34-35 0.0 5.477468160846948E-5 0.0 0.012625564110752215 0.0 36-37 0.0 5.477468160846948E-5 0.0 0.015008262760720637 0.0 38-39 0.0 5.477468160846948E-5 0.0 0.01665150320897472 0.0 40-41 0.0 5.477468160846948E-5 0.0 0.019828434742265953 0.0 42-43 0.0 5.477468160846948E-5 0.0 0.02426518395255198 0.0 44-45 0.0 5.477468160846948E-5 0.0 0.028564996458816834 0.0 46-47 0.0 5.477468160846948E-5 0.0 0.03530228229665858 0.0 48-49 0.0 5.477468160846948E-5 0.0 0.04327199847069089 0.0 50-51 0.0 5.477468160846948E-5 0.0 0.05567846385500923 0.0 52-53 0.0 5.477468160846948E-5 0.0 0.08651660960057754 0.0 54-55 0.0 5.477468160846948E-5 0.0 0.1271046486724534 0.0 56-57 0.0 5.477468160846948E-5 0.0 0.17648402414248865 0.0 58-59 0.0 5.477468160846948E-5 0.0 0.24897831525129804 0.0 60-61 0.0 5.477468160846948E-5 0.0 0.3511057091102894 0.0 62-63 0.0 5.477468160846948E-5 0.0 0.42140901295475997 0.0 64-65 0.0 5.477468160846948E-5 0.0 0.4938759167227651 0.0 66-67 0.0 5.477468160846948E-5 0.0 0.6307030713807218 0.0 68-69 0.0 5.477468160846948E-5 0.0 0.8056807917789777 0.0 70-71 0.0 5.477468160846948E-5 0.0 0.9597893584844064 0.0 72-73 0.0 5.477468160846948E-5 0.0 1.1207721477316983 0.0 74-75 0.0 5.477468160846948E-5 0.0 1.3982606847602046 0.0 76-77 0.0 5.477468160846948E-5 0.0 1.61566139606422 0.0 78-79 0.0 5.477468160846948E-5 0.0 1.7982801845468572 0.0 80-81 0.0 5.477468160846948E-5 0.0 2.0311821307460693 0.0 82-83 0.0 5.477468160846948E-5 0.0 2.4241904712868383 5.477468160846948E-5 84-85 0.0 5.477468160846948E-5 0.0 2.8257984368401363 5.477468160846948E-5 86-87 0.0 5.477468160846948E-5 0.0 3.3030776250355354 5.477468160846948E-5 88-89 0.0 5.477468160846948E-5 0.0 3.89680778633054 5.477468160846948E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7235 0.0 45.385574 1 GTATCAA 12465 0.0 39.19035 1 GCGTATC 70 2.0486768E-4 33.943005 1 TATCAAC 16075 0.0 30.35055 2 ATCAACG 16090 0.0 30.144278 3 TCAACGC 16225 0.0 29.810537 4 CAACGCA 16460 0.0 29.328812 5 AACGCAG 16880 0.0 28.620138 6 GTACATG 11880 0.0 28.560122 1 TACATGG 12050 0.0 27.59676 2 ACATGGG 12100 0.0 25.99009 3 ACGCAGA 18785 0.0 25.466272 7 CGCAGAG 18780 0.0 25.422459 8 GCAGAGT 19285 0.0 24.19017 9 GTGCGAA 120 1.7099228E-4 23.752926 9 GAGTACT 10985 0.0 23.134762 12-13 AGTACTT 11195 0.0 22.95538 12-13 CATGGGG 4270 0.0 22.810389 4 CATGGGA 7985 0.0 22.789278 4 GTACTTT 11660 0.0 21.79399 14-15 >>END_MODULE