FastQCFastQC Report
Thu 26 May 2016
SRR937423_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937423_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1825661
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156450.856949893764505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124060.6795347000346724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89730.49149321807279667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.15933954879903772No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.11250719602379632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81150.046.3810961
GTATCAA136200.038.0324021
TATCAAC178450.028.9594732
ATCAACG180900.028.5672633
TCAACGC182450.028.324574
CAACGCA185400.027.8738825
AACGCAG189700.027.2670976
GTACATG126350.024.4479521
ACGCAGA213850.024.100977
CGCAGAG214750.023.9778428
TACATGG127900.023.6191842
GTAAGGT7500.022.7991964
GAGTACT129600.022.7981412-13
ACATGGG126950.022.7483123
GCAGAGT225400.022.5498629
AGTACTT130650.022.5240212-13
TAAGGTG7600.021.8742315
GTACTTT136250.021.49190314-15
CATGGGG42800.020.6417984
GGTAAGG7500.020.2659533
Produced by FastQC (version 0.11.5)