##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937423_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1825661 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25033289312748 33.0 31.0 34.0 31.0 34.0 2 32.43769023931606 34.0 31.0 34.0 31.0 34.0 3 32.489109971676015 34.0 31.0 34.0 31.0 34.0 4 36.127627746881814 37.0 35.0 37.0 35.0 37.0 5 36.06937596848484 37.0 35.0 37.0 35.0 37.0 6 36.11853624522844 37.0 35.0 37.0 35.0 37.0 7 36.05648419942147 37.0 35.0 37.0 35.0 37.0 8 36.0366760313114 37.0 35.0 37.0 35.0 37.0 9 37.725325786112535 39.0 37.0 39.0 35.0 39.0 10-11 37.652796986954314 39.0 37.0 39.0 35.0 39.0 12-13 37.539029425506705 39.0 37.0 39.0 35.0 39.0 14-15 38.73328810770455 40.0 38.0 41.0 34.5 41.0 16-17 38.704173447315796 40.0 38.0 41.0 34.5 41.0 18-19 38.738589201390624 40.0 38.0 41.0 35.0 41.0 20-21 38.658423168375734 40.0 38.0 41.0 34.0 41.0 22-23 38.59371455051075 40.0 38.0 41.0 34.0 41.0 24-25 38.61136185743136 40.0 38.0 41.0 34.0 41.0 26-27 38.44939695814283 40.0 38.0 41.0 34.0 41.0 28-29 38.281426562762746 40.0 38.0 41.0 34.0 41.0 30-31 38.25166994310554 40.0 37.5 41.0 34.0 41.0 32-33 38.08056561431722 40.0 37.0 41.0 34.0 41.0 34-35 37.92330558630545 40.0 37.0 41.0 33.0 41.0 36-37 37.727908960097196 40.0 37.0 41.0 33.0 41.0 38-39 37.58019478972274 40.0 37.0 41.0 33.0 41.0 40-41 37.35317016686011 40.0 36.0 41.0 33.0 41.0 42-43 37.09647738545108 40.0 36.0 41.0 32.5 41.0 44-45 36.68247500494341 39.5 36.0 41.0 31.0 41.0 46-47 36.17556682209896 39.0 35.0 41.0 30.0 41.0 48-49 35.7190280670946 39.0 35.0 41.0 28.5 41.0 50-51 35.411793591471806 39.0 35.0 40.5 27.0 41.0 52-53 35.13886258182653 39.0 35.0 40.0 26.5 41.0 54-55 34.90229374456703 38.5 35.0 40.0 26.0 41.0 56-57 34.600262315950225 38.0 34.0 40.0 25.0 41.0 58-59 34.32754547531004 38.0 34.0 40.0 25.0 41.0 60-61 33.98041010899614 37.5 34.0 40.0 23.5 41.0 62-63 33.59640617836499 37.0 33.0 40.0 22.5 41.0 64-65 33.173044721884295 36.0 33.0 39.0 21.5 41.0 66-67 32.73698868519402 36.0 32.5 39.0 20.0 40.0 68-69 32.29730629070786 35.0 32.0 38.5 18.5 40.0 70-71 31.86603975217743 35.0 31.0 38.0 17.0 40.0 72-73 31.39287934616558 35.0 31.0 37.0 12.5 39.5 74-75 30.921590043277476 35.0 31.0 37.0 8.0 39.0 76-77 29.630852332388105 33.5 29.5 35.5 7.0 37.5 78-79 30.264955542129673 34.0 30.0 36.0 7.0 38.0 80-81 30.081538412662592 34.0 30.0 35.5 2.0 37.0 82-83 29.69916265944225 34.0 30.0 35.0 2.0 37.0 84-85 29.285339118270038 34.0 29.5 35.0 2.0 36.0 86-87 28.91572312713039 34.0 29.0 35.0 2.0 36.0 88-89 28.486489824781273 34.0 29.0 35.0 2.0 35.5 90-91 27.95861909741184 33.5 27.5 35.0 2.0 35.0 92-93 27.478100808419526 33.0 27.0 35.0 2.0 35.0 94-95 27.008196209482485 33.0 26.0 35.0 2.0 35.0 96-97 26.360106284792195 33.0 25.0 34.5 2.0 35.0 98-99 25.42895148661225 32.0 22.0 34.0 2.0 35.0 100-101 22.55800775719041 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1440648228757624 1101 2 0.1946941106096034 1101 3 0.15123207892884238 1101 4 0.11577866544650561 1101 5 0.08924421070801714 1101 6 0.12563793582603466 1101 7 0.09043102355027344 1101 8 0.12113686965869164 1101 9 0.22004808481253946 1101 10-11 0.16271916930651287 1101 12-13 0.18649437750556075 1101 14-15 0.28344094707382084 1101 16-17 0.21112335756124168 1101 18-19 0.18373765530153463 1101 20-21 0.2541371475513259 1101 22-23 0.3328305902377906 1101 24-25 0.2060283470334312 1101 26-27 0.10974275136442913 1101 28-29 0.04433055637732508 1101 30-31 0.24062797701395766 1101 32-33 0.2938762652730915 1101 34-35 0.24300103904840142 1101 36-37 0.3371785174851638 1101 38-39 0.2515440172346288 1101 40-41 0.2343899401054088 1101 42-43 0.1371768271578091 1101 44-45 0.20099959208465634 1101 46-47 0.18023524373535338 1101 48-49 0.17739233873624727 1101 50-51 0.10589912613042429 1101 52-53 0.041195373010850744 1101 54-55 0.026782126537781892 1101 56-57 0.1196603437412449 1101 58-59 0.22091349390009896 1101 60-61 0.2126748690490885 1101 62-63 0.20954943111402713 1101 64-65 0.1722397560840605 1101 66-67 0.10813809826831289 1101 68-69 0.18191638906566965 1101 70-71 0.19642571571423773 1101 72-73 0.12193092118275572 1101 74-75 0.4366098664005058 1101 76-77 0.39776052641486714 1101 78-79 0.3540699063951749 1101 80-81 0.3731190623052818 1101 82-83 0.1746076099631857 1101 84-85 0.34211787048554143 1101 86-87 0.5111147220500492 1101 88-89 0.7174361780232132 1101 90-91 0.7135131241684292 1101 92-93 0.7130667847412973 1101 94-95 0.9464006368684075 1101 96-97 1.106994843138935 1101 98-99 1.492646800551757 1101 100-101 2.166055927589838 1102 1 0.1318980000787775 1102 2 0.12787971525402497 1102 3 0.10770616619658568 1102 4 0.08733284126745389 1102 5 0.10595379570942498 1102 6 0.07274294361855027 1102 7 0.08861669120692994 1102 8 0.04990867714370495 1102 9 0.08412774908334342 1102 10-11 0.12922879547591037 1102 12-13 0.05691265273460289 1102 14-15 0.1617855315921375 1102 16-17 0.22763529417933626 1102 18-19 0.16946979448297128 1102 20-21 0.14296906871041415 1102 22-23 0.07808282822241353 1102 24-25 0.013566921024505518 1102 26-27 0.1186688548137198 1102 28-29 0.09415853297037557 1102 30-31 0.06334283824000408 1102 32-33 0.09624678562814637 1102 34-35 0.20018044328870843 1102 36-37 0.330882124725683 1102 38-39 0.22811627756758668 1102 40-41 0.23918622154326385 1102 42-43 0.31109669239563686 1102 44-45 0.18217552645110402 1102 46-47 0.3592628809024916 1102 48-49 0.5650148450269725 1102 50-51 0.4164559862726591 1102 52-53 0.49610549655683656 1102 54-55 0.46204797516558926 1102 56-57 0.49205867349688504 1102 58-59 0.5128172616117865 1102 60-61 0.6270349377744324 1102 62-63 0.5945785637715844 1102 64-65 0.6321818268342057 1102 66-67 0.7028349884043763 1102 68-69 0.4690688240685432 1102 70-71 0.48097889594184196 1102 72-73 0.6022379168013039 1102 74-75 0.428869023128744 1102 76-77 0.4851876947709606 1102 78-79 0.5293640685214562 1102 80-81 0.6724780178144378 1102 82-83 0.6057831242480596 1102 84-85 0.6456284922482887 1102 86-87 0.8635129677893687 1102 88-89 0.9194557202183056 1102 90-91 1.061561215107691 1102 92-93 1.0002429543534355 1102 94-95 1.1005670059772896 1102 96-97 1.2376190003502643 1102 98-99 1.7882878240385196 1102 100-101 2.4198328810742034 1103 1 0.17696813228095465 1103 2 0.1863757436916984 1103 3 0.2304210290929447 1103 4 0.12223174278919657 1103 5 0.08115283190698364 1103 6 0.08963834540140425 1103 7 0.09899314217852151 1103 8 0.10376480306101854 1103 9 0.1833851997709246 1103 10-11 0.18620972098278088 1103 12-13 0.19343178677826955 1103 14-15 0.1508699192752445 1103 16-17 0.13123237528716203 1103 18-19 0.24006740696476214 1103 20-21 0.20680196992066158 1103 22-23 0.2068555833964396 1103 24-25 0.10340004211363407 1103 26-27 0.13985945315913284 1103 28-29 0.13456127779177507 1103 30-31 0.10485940703003394 1103 32-33 0.17596318767667896 1103 34-35 0.21484567768875706 1103 36-37 0.17706218720793743 1103 38-39 0.12083759940614414 1103 40-41 -0.010448458853616671 1103 42-43 0.18772585749719184 1103 44-45 0.2927123057751615 1103 46-47 0.19987107505554746 1103 48-49 0.06379543338621119 1103 50-51 0.09617261736170235 1103 52-53 0.06248853473331195 1103 54-55 0.06761824021126017 1103 56-57 0.084786179429031 1103 58-59 0.08656280677398343 1103 60-61 0.12866570615951645 1103 62-63 0.07153182118562995 1103 64-65 0.05014982529505119 1103 66-67 0.1932439173193714 1103 68-69 0.09566271693255857 1103 70-71 0.03964624667614558 1103 72-73 -0.07446477030188348 1103 74-75 0.035036625807599364 1103 76-77 8.810919913280202E-4 1103 78-79 0.04862500062469266 1103 80-81 0.08001986351443335 1103 82-83 0.03317407806433437 1103 84-85 0.05717098842331225 1103 86-87 0.09248407203944353 1103 88-89 0.2586182107131272 1103 90-91 0.4670774265945319 1103 92-93 0.3492777776715883 1103 94-95 0.3624349687078201 1103 96-97 0.5036090050226925 1103 98-99 0.7642974345780473 1103 100-101 1.4433436597202984 1104 1 0.1410113556199022 1104 2 0.22283826188508016 1104 3 0.17782781241150758 1104 4 0.09146966399177359 1104 5 0.10076334435194667 1104 6 0.05926607555626617 1104 7 0.16096266453629227 1104 8 0.07917401063313179 1104 9 0.27392670822596443 1104 10-11 0.10093993094701403 1104 12-13 0.184096803605712 1104 14-15 0.1930969267454401 1104 16-17 0.17937963491645093 1104 18-19 0.16619769222777592 1104 20-21 0.2664676479081649 1104 22-23 0.22577176299321877 1104 24-25 0.13156534590291358 1104 26-27 0.18459301483756008 1104 28-29 0.09380466274762256 1104 30-31 0.1159348711047059 1104 32-33 0.12590430310880407 1104 34-35 0.11654414737870411 1104 36-37 0.23903125042677686 1104 38-39 0.3220057636309548 1104 40-41 0.2843193086751583 1104 42-43 0.26924528596105546 1104 44-45 0.3015750645573405 1104 46-47 0.36981779198262643 1104 48-49 0.5262296043419994 1104 50-51 0.47752813322725274 1104 52-53 0.4525597701384356 1104 54-55 0.4093702684574865 1104 56-57 0.3898013539706966 1104 58-59 0.27625421949856843 1104 60-61 0.41212981756498124 1104 62-63 0.4976149299235004 1104 64-65 0.33734088218695746 1104 66-67 0.4845098322225567 1104 68-69 0.6561081276093788 1104 70-71 0.5552581181723433 1104 72-73 0.6883997286168473 1104 74-75 0.5292170398590876 1104 76-77 0.5697445853957959 1104 78-79 0.5214921855061654 1104 80-81 0.5370462961426803 1104 82-83 0.44136810099373136 1104 84-85 0.48241662775131644 1104 86-87 0.5360169854137027 1104 88-89 0.7167354426563399 1104 90-91 0.7064628232216954 1104 92-93 0.7012114412788613 1104 94-95 0.715418912619409 1104 96-97 0.8231741774982311 1104 98-99 0.9464225820764298 1104 100-101 1.8149143510457861 1105 1 0.1876541312297917 1105 2 0.1257715527454195 1105 3 0.13491841260794502 1105 4 0.08413457864760687 1105 5 0.12215486568135248 1105 6 0.04937623987365214 1105 7 0.02349989476643799 1105 8 0.08974341415308373 1105 9 0.11727039435056952 1105 10-11 0.1665829985587024 1105 12-13 0.16847417612037674 1105 14-15 0.199813499175157 1105 16-17 0.25980701937664463 1105 18-19 0.288818806612845 1105 20-21 0.2981992186124174 1105 22-23 0.2979694747608974 1105 24-25 0.2135822820076072 1105 26-27 0.18201331161757395 1105 28-29 0.18002801758291298 1105 30-31 0.33788915954939824 1105 32-33 0.36925436018187696 1105 34-35 0.28688048036694624 1105 36-37 0.3938049490278175 1105 38-39 0.3314400764976142 1105 40-41 0.31150202216829825 1105 42-43 0.05436487397305001 1105 44-45 0.1458536873706464 1105 46-47 0.14117029125262093 1105 48-49 0.22867923403062207 1105 50-51 0.14945773424659592 1105 52-53 0.22588779434803996 1105 54-55 0.2977018763256041 1105 56-57 0.3014320513240989 1105 58-59 0.13978691042792946 1105 60-61 0.3221014137530389 1105 62-63 0.3048894912571569 1105 64-65 0.3500349970233074 1105 66-67 0.3854437070865089 1105 68-69 0.3318549885899671 1105 70-71 0.338535440250336 1105 72-73 0.3909550098676746 1105 74-75 0.3774881237166987 1105 76-77 0.42745635306853913 1105 78-79 0.3440963106953028 1105 80-81 0.30406867409920224 1105 82-83 0.3225456646368521 1105 84-85 0.4995842102418244 1105 86-87 0.7352503479472183 1105 88-89 1.0525083183432073 1105 90-91 1.1274407638125972 1105 92-93 1.2126122535747932 1105 94-95 1.2844040736185853 1105 96-97 1.479813502177695 1105 98-99 1.6596157545433137 1105 100-101 2.123579371884098 1106 1 -0.033468695786453395 1106 2 0.13093921684725274 1106 3 0.08277440196863495 1106 4 0.09223185232482223 1106 5 0.046532181155846786 1106 6 0.08784113965516127 1106 7 0.022314371825430612 1106 8 -0.008206619094757173 1106 9 0.013575134542456624 1106 10-11 -0.16632096268381247 1106 12-13 -0.2837971262449699 1106 14-15 -0.07841771579587231 1106 16-17 -0.02394523672671056 1106 18-19 0.1488245062423701 1106 20-21 0.07056361492666241 1106 22-23 0.06128095951438439 1106 24-25 0.20702580817840044 1106 26-27 0.14706128349939718 1106 28-29 0.1369160198627597 1106 30-31 0.19374742876189543 1106 32-33 0.26418358978195755 1106 34-35 -0.31202519076443025 1106 36-37 0.1717321837575625 1106 38-39 0.3566134402289691 1106 40-41 0.2913253336530559 1106 42-43 0.250111050471574 1106 44-45 0.5615143694263125 1106 46-47 0.41074946860609174 1106 48-49 0.4907045023881764 1106 50-51 0.6226295780767472 1106 52-53 0.5632507657548942 1106 54-55 0.5500073764681019 1106 56-57 0.5336653561592826 1106 58-59 0.5421351577918472 1106 60-61 0.6167646606419837 1106 62-63 0.6952590172379587 1106 64-65 0.4374293163925458 1106 66-67 0.5205477253253292 1106 68-69 0.5686536966739482 1106 70-71 0.6447974332207842 1106 72-73 0.7049172023251664 1106 74-75 0.8139904510654183 1106 76-77 0.5657179660636977 1106 78-79 0.619983078055423 1106 80-81 0.6689589562308633 1106 82-83 0.689223730562567 1106 84-85 0.9086523208146353 1106 86-87 0.9089427579405687 1106 88-89 0.9584929293434925 1106 90-91 1.042029915517741 1106 92-93 0.9257916969109736 1106 94-95 0.5713443225024086 1106 96-97 1.142916000515303 1106 98-99 1.0933480619433347 1106 100-101 1.4674627194665035 1107 1 -0.03973777478280738 1107 2 0.07127128445205244 1107 3 7.021017353281422E-5 1107 4 -0.012510906963392188 1107 5 -0.03822695814874777 1107 6 0.008963915323391802 1107 7 0.03799451477830473 1107 8 -0.04311094490817169 1107 9 0.012234951384094472 1107 10-11 0.025727283909887433 1107 12-13 -0.007436468066124746 1107 14-15 0.02385315011136413 1107 16-17 -0.019678987005910642 1107 18-19 0.05213773682832823 1107 20-21 0.10788771588712365 1107 22-23 0.09658734027531324 1107 24-25 -0.06034073191541012 1107 26-27 -0.07980938882581512 1107 28-29 0.027482619520270646 1107 30-31 0.016791133623783594 1107 32-33 0.030708792670793628 1107 34-35 -0.03026657264217647 1107 36-37 8.36494680051203E-4 1107 38-39 -0.023574185413202997 1107 40-41 -0.11400561713244173 1107 42-43 -0.08788314206807257 1107 44-45 -0.03109228681670828 1107 46-47 -0.028212633992374947 1107 48-49 0.04747615006891692 1107 50-51 0.05403453675443615 1107 52-53 0.08997997430228111 1107 54-55 0.07911168739059349 1107 56-57 0.15105050990807456 1107 58-59 0.18318943520976205 1107 60-61 0.25687336438705444 1107 62-63 0.05722759738836203 1107 64-65 0.15654181732322314 1107 66-67 0.18283646034133483 1107 68-69 0.25697176681019585 1107 70-71 0.27679802885774407 1107 72-73 0.2025272090260799 1107 74-75 0.27744067815201134 1107 76-77 0.25657493873885073 1107 78-79 0.26688204313620645 1107 80-81 0.315887703159607 1107 82-83 0.3888886847729758 1107 84-85 0.36501446808587446 1107 86-87 0.6083396755193036 1107 88-89 0.8307511379653398 1107 90-91 0.7648651474438815 1107 92-93 0.8892419239975631 1107 94-95 0.8158830761320708 1107 96-97 1.0320307350845823 1107 98-99 1.1586894363756137 1107 100-101 1.5552074890294563 1108 1 0.07961558985357442 1108 2 0.20516864665129475 1108 3 0.21214925810163976 1108 4 0.12991609489757394 1108 5 0.14412004212277907 1108 6 0.1696823401927645 1108 7 0.1272307247692268 1108 8 0.05146941099293656 1108 9 0.055895421459659644 1108 10-11 0.0966359774600889 1108 12-13 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2316 80-81 -0.19557765658161586 2316 82-83 -0.04238802236579886 2316 84-85 0.08382824207206241 2316 86-87 0.11489667862803188 2316 88-89 0.18663740764471015 2316 90-91 0.2544434163324958 2316 92-93 0.2922055338577252 2316 94-95 0.36721574470611884 2316 96-97 0.49196204329174975 2316 98-99 0.589854481333763 2316 100-101 1.2793530235198176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 13.0 5 50.0 6 87.0 7 205.0 8 483.0 9 943.0 10 1723.0 11 3027.0 12 4785.0 13 7035.0 14 9031.0 15 12827.0 16 18597.0 17 27768.0 18 24408.0 19 13163.0 20 10628.0 21 10568.0 22 10524.0 23 11306.0 24 12828.0 25 14704.0 26 17924.0 27 22244.0 28 27447.0 29 33591.0 30 42129.0 31 53419.0 32 68543.0 33 90442.0 34 126542.0 35 183732.0 36 276297.0 37 391503.0 38 276612.0 39 20525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85480768703867 19.76047871378549 15.451616261802082 20.93309733737376 2 15.574139996417735 21.47309933224186 39.92970217362369 23.02305849771672 3 18.815431780598917 26.52299632845309 30.723776210369834 23.93779568057816 4 12.609843777130584 17.636680632384653 37.36953355524383 32.383942035240935 5 12.865911573218467 38.862682031456266 33.397219395216815 14.874187000108455 6 30.308091151643158 37.50334810241332 17.33520078481164 14.853359961131888 7 27.545037112585526 34.98771130018114 20.195479883724307 17.27177170350903 8 27.12601079828073 34.468173445124805 19.325986587871462 19.079829168723002 9 27.69051866693762 16.22393204433901 20.10061013517844 35.984939153544936 10-11 24.88838289255234 27.68890281383017 26.39769924427372 21.02501504934377 12-13 26.371108727083598 25.23552162044243 27.61964651694767 20.773723135526303 14-15 22.993617106352165 25.910149803276735 27.09966417642706 23.996568913944046 16-17 21.194980886374854 29.588077961899828 27.39585826722486 21.821082884500463 18-19 21.152557895469094 26.829241573326044 31.593625541653132 20.42457498955173 20-21 22.26748744768671 25.788653360607494 31.85506388239428 20.08879530931151 22-23 21.84119181726608 25.959891523507533 31.658714243537506 20.540202415688885 24-25 21.46142844857665 26.1662774921275 31.875859621101206 20.496434438194647 26-27 21.280504638414044 26.43027808573945 31.69647747838547 20.592739797461032 28-29 20.962601761399302 26.572255386465994 31.9027396014667 20.562403250668 30-31 21.082963854372387 26.35186301475661 31.86415407279518 20.701019058075822 32-33 21.10279082785997 26.464772446552175 31.578460161508804 20.85397656407905 34-35 21.35289629345207 26.450830685433935 31.427713031061078 20.768559990052918 36-37 21.629289336848405 26.189363742775907 31.386330759105878 20.795016161269807 38-39 21.356620971801448 26.41706209422231 31.382414369370874 20.84390256460537 40-41 21.40605512195309 26.507029508764223 31.30438783541961 20.782527533863078 42-43 21.33882366217058 26.68986745095762 30.867323322266127 21.103985564605676 44-45 21.356785295846272 26.970998449328764 30.52198628332423 21.150229971500735 46-47 21.720133146296053 27.245200505460758 29.776913676745025 21.25775267149816 48-49 21.954787882306736 27.47303579361119 29.253733305361727 21.31844301872034 50-51 22.044481423440608 27.721795010136052 28.81786377646233 21.415859789961004 52-53 22.163890229347068 27.96105081940185 28.57945697476147 21.29560197648961 54-55 21.63901184283391 27.901017768358965 28.720857815333733 21.73911257347339 56-57 21.85701507563562 28.191707003655115 28.48349173258343 21.467786188125835 58-59 21.984859182509787 27.740938761358215 28.648308749543318 21.62589330658868 60-61 21.912720926831433 27.74447172832196 29.05377833015001 21.289029014696595 62-63 21.918171007651473 27.733023819865792 29.134625760204113 21.21417941227862 64-65 21.72139296397305 27.73781660450653 29.08869718967541 21.45209324184501 66-67 21.636766080887963 28.13884943590294 28.66282404016956 21.561560443039536 68-69 21.727171692882745 28.060987225996502 28.730607708660045 21.481233372460714 70-71 21.640600308600554 28.19847167683376 28.606570442157665 21.55435757240802 72-73 21.80539488271613 28.142925063648356 28.351343297970704 21.70033675566481 74-75 21.652760287917637 28.40039854058338 28.100534546117817 21.84630662538116 76-77 21.666727831727798 28.1812724268087 28.41770733997166 21.734292401491842 78-79 21.719037652663882 28.099521214508062 28.30873311091161 21.872708021916445 80-81 21.604640730124597 28.61371853810757 28.05857714000573 21.723063591762106 82-83 21.562775671532773 28.64938100315202 27.925147981095012 21.8626953442202 84-85 21.55270465275699 28.320532936180047 27.971782251838306 22.154980159224653 86-87 21.471483479134406 28.151365450650477 28.03970726219161 22.337443808023504 88-89 21.50492342225638 28.02061828565106 28.046280223984628 22.428178068107936 90-91 21.425492030659065 28.209346939133255 28.177766481000234 22.187394549207447 92-93 21.33432476511694 27.86733985432526 28.275935732214823 22.52239964834298 94-95 21.099672940376117 27.847393355064277 28.283016397896432 22.769917306663174 96-97 21.30989816838942 27.89208949525679 28.1385755624949 22.659436773858893 98-99 21.058051398454694 27.888191545185354 27.9603299194044 23.09342713695555 100-101 20.765551785691297 28.126264248950182 28.26444999743898 22.84373396791954 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1084.0 1 766.0 2 540.0 3 673.5 4 648.5 5 666.5 6 1122.0 7 2401.5 8 3941.5 9 4719.5 10 4461.5 11 4066.0 12 4111.0 13 4376.0 14 4747.5 15 5015.5 16 5206.0 17 5362.0 18 5627.0 19 5832.0 20 6131.5 21 6676.5 22 7512.0 23 8675.0 24 10025.0 25 11852.5 26 14394.5 27 17176.5 28 19612.5 29 22272.0 30 25429.5 31 28490.5 32 31567.0 33 34261.0 34 38293.5 35 41318.5 36 44153.5 37 48447.5 38 50615.5 39 51148.5 40 51776.5 41 54408.5 42 57609.5 43 60051.5 44 61927.5 45 63574.5 46 62804.0 47 63177.0 48 68657.0 49 68535.0 50 63754.5 51 60504.0 52 57881.0 53 56679.5 54 54595.0 55 50510.0 56 45221.0 57 40086.5 58 37142.0 59 33906.5 60 29143.0 61 24430.5 62 19987.0 63 15336.0 64 10721.0 65 8004.5 66 6062.5 67 4726.5 68 3368.5 69 2014.5 70 1446.5 71 1107.5 72 909.0 73 797.0 74 621.0 75 460.0 76 330.5 77 200.0 78 122.5 79 84.0 80 45.5 81 25.0 82 14.0 83 6.5 84 3.5 85 3.0 86 3.0 87 3.0 88 2.5 89 2.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04223127952012997 2 0.0 3 0.0 4 0.0 5 8.216202241270421E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004683235277524141 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.216202241270422E-5 22-23 6.572961793016338E-4 24-25 1.0954936321693895E-4 26-27 0.0020266632195133708 28-29 0.003669903667767455 30-31 2.4648606723811267E-4 32-33 1.0954936321693895E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.008106652878053483 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.738734080423474E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.0126074884658215E-4 84-85 8.216202241270422E-5 86-87 0.0 88-89 0.0 90-91 0.009037822465397465 92-93 0.0010133316097566854 94-95 0.0 96-97 0.0 98-99 1.6432404482540845E-4 100-101 0.01202304261305905 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1825661.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.8426611688634 #Duplication Level Percentage of deduplicated Percentage of total 1 71.26182289474777 33.38093424134222 2 14.792373049143706 13.858282372489308 3 5.454307284988383 7.6648280418472226 4 2.6579683339298223 4.980252402553722 5 1.5127317977187482 3.5430191519952468 6 0.9516398861568826 2.6746406845213557 7 0.636552062455722 2.087245480456819 8 0.4416214396117927 1.6549378768512697 9 0.33342863509477033 1.405681611996683 >10 1.7550847715573292 14.99786824890284 >50 0.1283247941021346 4.122164897494172 >100 0.06932780598765778 5.804288523986832 >500 0.003289825515832852 1.0130698846506598 >1k 0.0011749376842260185 0.7750889979646461 >5k 1.1749376842260186E-4 0.493848865918964 >10k+ 2.349875368452037E-4 1.5438487170280686 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15645 0.856949893764505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12406 0.6795347000346724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8973 0.49149321807279667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2909 0.15933954879903772 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2054 0.11250719602379632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 8.216202241270422E-5 0.0 20-21 0.0 0.0 0.0 1.0954936321693897E-4 0.0 22-23 0.0 0.0 0.0 1.917113856296432E-4 0.0 24-25 0.0 0.0 0.0 3.834227712592864E-4 0.0 26-27 0.0 0.0 0.0 7.394582017143381E-4 0.0 28-29 0.0 0.0 0.0 0.0018623391746879622 0.0 30-31 0.0 0.0 0.0 0.0055870175240638874 0.0 32-33 0.0 0.0 0.0 0.009640343963090629 0.0 34-35 0.0 0.0 0.0 0.012817275496381859 0.0 36-37 0.0 0.0 0.0 0.015336910850371456 0.0 38-39 0.0 0.0 0.0 0.017144475343450948 0.0 40-41 0.0 0.0 0.0 0.020348794217546415 0.0 42-43 0.0 0.0 0.0 0.024593832042202796 0.0 44-45 0.0 0.0 0.0 0.02894841923007612 0.0 46-47 0.0 0.0 0.0 0.03585002911274328 0.0 48-49 0.0 0.0 0.0 0.04381974528677558 0.0 50-51 0.0 0.0 0.0 0.05600711194466004 0.0 52-53 0.0 0.0 0.0 0.08659877162299025 0.0 54-55 0.0 0.0 0.0 0.12751545878451695 0.0 56-57 0.0 0.0 0.0 0.1776069051154623 0.0 58-59 0.0 0.0 0.0 0.25089542910759444 0.0 60-61 0.0 0.0 0.0 0.3540361545763425 0.0 62-63 0.0 0.0 0.0 0.42524324066735286 0.0 64-65 0.0 0.0 0.0 0.4982578912514426 0.0 66-67 0.0 0.0 0.0 0.6352767572950291 0.0 68-69 0.0 0.0 0.0 0.8119251054823431 0.0 70-71 0.0 0.0 0.0 0.9680603354072854 0.0 72-73 0.0 0.0 0.0 1.1302481676499636 0.0 74-75 0.0 0.0 0.0 1.4125842640008193 0.0 76-77 0.0 0.0 0.0 1.6340109144030572 0.0 78-79 0.0 0.0 0.0 1.8199983458046154 0.0 80-81 0.0 0.0 0.0 2.055967674173902 0.0 82-83 0.0 0.0 0.0 2.456014561301359 0.0 84-85 0.0 0.0 0.0 2.866578187297642 0.0 86-87 0.0 0.0 0.0 3.353442944774523 0.0 88-89 0.0 0.0 0.0 3.9654678497267564 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8115 0.0 46.381096 1 GTATCAA 13620 0.0 38.032402 1 TATCAAC 17845 0.0 28.959473 2 ATCAACG 18090 0.0 28.567263 3 TCAACGC 18245 0.0 28.32457 4 CAACGCA 18540 0.0 27.873882 5 AACGCAG 18970 0.0 27.267097 6 GTACATG 12635 0.0 24.447952 1 ACGCAGA 21385 0.0 24.10097 7 CGCAGAG 21475 0.0 23.977842 8 TACATGG 12790 0.0 23.619184 2 GTAAGGT 750 0.0 22.799196 4 GAGTACT 12960 0.0 22.79814 12-13 ACATGGG 12695 0.0 22.748312 3 GCAGAGT 22540 0.0 22.549862 9 AGTACTT 13065 0.0 22.52402 12-13 TAAGGTG 760 0.0 21.874231 5 GTACTTT 13625 0.0 21.491903 14-15 CATGGGG 4280 0.0 20.641798 4 GGTAAGG 750 0.0 20.265953 3 >>END_MODULE