FastQCFastQC Report
Thu 26 May 2016
SRR937422_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937422_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814037
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149690.8251761127253744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106270.5858204656244608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77400.42667266433926104No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24090.13279773235055295No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23560.1298760719875063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18220.10043896568813093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71200.044.545691
GTATCAA126550.041.2948041
TATCAAC161100.032.3393142
ATCAACG162150.032.0996973
TCAACGC163750.031.7886874
CAACGCA165500.031.4508135
AACGCAG169950.030.6052996
ACGCAGA189850.027.2471457
CGCAGAG190250.027.0650188
GTACATG119600.025.7236711
GCAGAGT198250.025.5176169
TACATGG119950.024.847522
GAGTACT113150.023.8233612-13
ACATGGG121350.023.6200753
AGTACTT115950.022.85889212-13
GTACTTT119750.022.5698414-15
CATGGGA79450.021.1212964
AGAGTAC186950.020.97467810-11
CATGGGG40050.020.4157684
CAGAGTA197800.020.30444110-11
Produced by FastQC (version 0.11.5)