##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937422_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1814037 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.771826594496144 33.0 31.0 34.0 30.0 34.0 2 31.85833365030592 33.0 31.0 34.0 30.0 34.0 3 31.878709199426474 33.0 31.0 34.0 30.0 34.0 4 35.52482005604075 37.0 35.0 37.0 33.0 37.0 5 35.53811361069261 37.0 35.0 37.0 33.0 37.0 6 35.59704405147194 37.0 35.0 37.0 33.0 37.0 7 35.540921712181174 37.0 35.0 37.0 33.0 37.0 8 35.539163754653295 37.0 35.0 37.0 33.0 37.0 9 37.15083485066732 39.0 37.0 39.0 34.0 39.0 10-11 37.03262006232508 39.0 37.0 39.0 33.0 39.0 12-13 36.8946308702634 39.0 37.0 39.0 33.0 39.0 14-15 38.029664224048354 40.0 38.0 41.0 33.0 41.0 16-17 38.00982339389991 40.0 38.0 41.0 33.0 41.0 18-19 38.035512230456156 40.0 38.0 41.0 33.0 41.0 20-21 38.00233071321037 40.0 38.0 41.0 33.0 41.0 22-23 37.854102755346226 40.0 38.0 41.0 33.0 41.0 24-25 37.688587663867935 40.0 37.0 41.0 32.5 41.0 26-27 37.51618958157965 40.0 37.0 41.0 32.0 41.0 28-29 37.54872089158049 40.0 37.0 41.0 32.0 41.0 30-31 37.341110738094095 39.5 37.0 41.0 32.0 41.0 32-33 37.08949321320348 39.0 36.0 41.0 31.5 41.0 34-35 36.93177233981446 39.0 36.0 41.0 31.0 41.0 36-37 36.90369160055721 39.0 36.0 41.0 31.0 41.0 38-39 36.79612736675161 39.0 36.0 41.0 31.0 41.0 40-41 36.576442487115756 39.0 35.0 41.0 30.5 41.0 42-43 36.277955466178476 39.0 35.0 41.0 30.0 41.0 44-45 35.956185017174406 39.0 35.0 41.0 29.5 41.0 46-47 35.467679545676305 39.0 35.0 41.0 26.5 41.0 48-49 35.03649181356279 39.0 35.0 40.5 24.5 41.0 50-51 34.11476860725553 38.0 34.0 39.5 23.5 40.5 52-53 34.182935353578785 38.0 34.0 39.5 23.0 40.5 54-55 34.482123021746524 38.0 34.0 40.0 23.0 41.0 56-57 34.282818928169604 38.0 34.0 40.0 22.5 41.0 58-59 34.03972493394567 38.0 34.0 40.0 21.5 41.0 60-61 33.758324389193824 38.0 34.0 40.0 20.0 41.0 62-63 33.42278024097634 37.0 33.0 40.0 18.5 41.0 64-65 33.05481475846413 37.0 33.0 40.0 17.5 41.0 66-67 32.685673996726635 36.0 32.5 39.0 13.5 41.0 68-69 32.22316358486624 36.0 32.0 39.0 9.0 40.5 70-71 31.789104632375192 35.0 31.5 38.5 7.5 40.0 72-73 31.411797278666313 35.0 31.0 38.0 4.5 40.0 74-75 30.96714041665082 35.0 31.0 37.0 2.0 39.0 76-77 30.539793014144696 35.0 30.5 36.5 2.0 39.0 78-79 30.077296383701103 34.5 30.0 36.0 2.0 39.0 80-81 29.611708581467745 34.0 29.5 36.0 2.0 37.5 82-83 29.15483311531132 34.0 29.0 35.0 2.0 37.0 84-85 28.745886660525667 34.0 29.0 35.0 2.0 36.5 86-87 28.324725736024128 34.0 28.5 35.0 2.0 36.0 88-89 27.933485645551883 34.0 27.0 35.0 2.0 36.0 90-91 27.5361585789044 34.0 26.5 35.0 2.0 35.0 92-93 27.11276699427851 33.0 26.0 35.0 2.0 35.0 94-95 26.608576616684225 33.0 25.0 35.0 2.0 35.0 96-97 26.010222779358966 33.0 24.5 35.0 2.0 35.0 98-99 25.285566115795874 32.0 20.5 34.5 2.0 35.0 100-101 23.420843400658313 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17036534539711923 1101 2 0.15707689883322473 1101 3 0.17215884708334883 1101 4 0.13975255133087927 1101 5 0.17524979705550692 1101 6 0.18109416195004258 1101 7 0.14640070785645776 1101 8 0.22648670646836422 1101 9 0.22914053271398416 1101 10-11 0.29108087046076747 1101 12-13 0.18513459323830972 1101 14-15 0.412051901808276 1101 16-17 0.514151448613184 1101 18-19 0.4528452664765794 1101 20-21 0.3995594806904137 1101 22-23 0.44284989992113566 1101 24-25 0.4239169026551082 1101 26-27 0.3186917444205406 1101 28-29 0.3650980923887417 1101 30-31 0.3925510833394412 1101 32-33 0.42179878232878565 1101 34-35 0.49663803052394684 1101 36-37 0.4764086523095088 1101 38-39 0.5606140095780106 1101 40-41 0.34868514013907514 1101 42-43 0.5379457035859332 1101 44-45 0.6366771710645196 1101 46-47 0.8197085905654049 1101 48-49 0.8409420009731079 1101 50-51 0.7771392763676346 1101 52-53 0.7543214263288931 1101 54-55 0.9290100256310723 1101 56-57 0.8280641861241307 1101 58-59 0.9786691678565091 1101 60-61 0.7365398427990044 1101 62-63 0.8224866052236806 1101 64-65 0.9121140931835754 1101 66-67 0.9053727292455136 1101 68-69 0.9136385235038631 1101 70-71 0.8529878330351757 1101 72-73 0.8455210164818929 1101 74-75 0.8514594349315949 1101 76-77 0.9433341692855528 1101 78-79 0.8291121068371758 1101 80-81 0.690862604940822 1101 82-83 0.7444924371630286 1101 84-85 0.8609729253231286 1101 86-87 0.8460685857568961 1101 88-89 0.7700255561865355 1101 90-91 0.8998157225308852 1101 92-93 0.9467719532514991 1101 94-95 0.9421008896959897 1101 96-97 1.2055958284409556 1101 98-99 1.3405162082674593 1101 100-101 1.7340871412124699 1102 1 -0.04280824950885531 1102 2 0.03880425876775462 1102 3 0.06718410297172639 1102 4 -0.06620695046125746 1102 5 -0.011961671067254542 1102 6 -0.03318540345951959 1102 7 0.006553922408947699 1102 8 0.028204724522900904 1102 9 0.0360869153411727 1102 10-11 0.08721856282339502 1102 12-13 0.11893482755736784 1102 14-15 0.1826379117639405 1102 16-17 0.26708941196753955 1102 18-19 0.21137841760464227 1102 20-21 0.1529603882424766 1102 22-23 0.08038206088713196 1102 24-25 0.05524366192991437 1102 26-27 0.11549321209937347 1102 28-29 0.14366947960002108 1102 30-31 0.10258559425149372 1102 32-33 0.11223435713427676 1102 34-35 0.14001484992262903 1102 36-37 0.2204602488252192 1102 38-39 0.3322519461034048 1102 40-41 0.2942906927672553 1102 42-43 0.43919768921796987 1102 44-45 0.36453334471539733 1102 46-47 0.4879151233189205 1102 48-49 0.3084136327331848 1102 50-51 0.2023322421636209 1102 52-53 0.2922067064984333 1102 54-55 0.2336815446709437 1102 56-57 0.20685744298530295 1102 58-59 0.3261035286036247 1102 60-61 0.3342966335157982 1102 62-63 0.18196842040500627 1102 64-65 0.3105040664897274 1102 66-67 0.3776876625495049 1102 68-69 0.37311290764428406 1102 70-71 0.34364305782898086 1102 72-73 0.3774624289973154 1102 74-75 0.3813109878261294 1102 76-77 0.42169980352006675 1102 78-79 0.31309165120821447 1102 80-81 0.2124294157889821 1102 82-83 0.18770065878948117 1102 84-85 0.24746367898508836 1102 86-87 0.2359591853936429 1102 88-89 0.317637393352161 1102 90-91 0.34548844404828216 1102 92-93 0.31697560237511624 1102 94-95 0.31847838271370676 1102 96-97 0.43164239887081735 1102 98-99 0.4956874406814684 1102 100-101 1.003936030513124 1103 1 0.06626265320432978 1103 2 -0.006573356085159077 1103 3 0.07016112934974572 1103 4 0.07701373370159814 1103 5 0.062014121215696605 1103 6 0.06638235930242331 1103 7 0.03750721525423728 1103 8 0.0624730520505139 1103 9 0.03215057762467666 1103 10-11 0.1803948681980927 1103 12-13 0.15422751478418206 1103 14-15 0.19382805359367694 1103 16-17 0.07305987879590958 1103 18-19 0.05187163008177009 1103 20-21 -0.14575232293672968 1103 22-23 -0.12540602735978723 1103 24-25 -0.22557497472939758 1103 26-27 -0.06906874494438853 1103 28-29 -0.08602418517599375 1103 30-31 -0.03274387265591372 1103 32-33 0.04035713439531463 1103 34-35 0.04356211333200832 1103 36-37 0.14977274236598248 1103 38-39 0.15201388126174464 1103 40-41 0.14458097867607478 1103 42-43 0.2414455052344593 1103 44-45 0.42860598002511097 1103 46-47 0.3684589762488386 1103 48-49 0.5184940008947478 1103 50-51 0.4757669779882434 1103 52-53 0.33818100327702183 1103 54-55 0.24070737555737054 1103 56-57 0.15031187569672255 1103 58-59 0.21365983799064736 1103 60-61 0.20157366050175085 1103 62-63 0.06402875123975349 1103 64-65 0.1974375530210679 1103 66-67 0.34031981102998543 1103 68-69 0.3907708019011835 1103 70-71 0.5205527135590167 1103 72-73 0.3892753936827411 1103 74-75 0.6879076211175175 1103 76-77 0.6998824617486825 1103 78-79 0.6594951455818325 1103 80-81 0.5655088781369209 1103 82-83 0.5302067228773168 1103 84-85 0.3275205811025046 1103 86-87 0.5696028662797588 1103 88-89 0.302908803077802 1103 90-91 0.5969751334514797 1103 92-93 0.5885379180967583 1103 94-95 0.7270589910070662 1103 96-97 0.7401226989443757 1103 98-99 0.7841334535817133 1103 100-101 1.1157663211637896 1104 1 0.1410987137546762 1104 2 -0.0029789453478912264 1104 3 0.024448069350974322 1104 4 0.025922846896420992 1104 5 0.07803422295008744 1104 6 -0.018513761307985988 1104 7 0.015297250187131795 1104 8 0.04423134095166148 1104 9 0.17858987406397375 1104 10-11 0.09850941131263369 1104 12-13 0.14657292141529865 1104 14-15 0.26216475764466907 1104 16-17 0.20296120869723921 1104 18-19 0.1887013169114553 1104 20-21 0.2210001438157434 1104 22-23 0.27470735132502 1104 24-25 0.31364945378074793 1104 26-27 0.15573667271817015 1104 28-29 0.10546934917469031 1104 30-31 0.21208973646340468 1104 32-33 0.11547204522451437 1104 34-35 0.15899233947652647 1104 36-37 0.36498540400721424 1104 38-39 0.23014098796027582 1104 40-41 0.35423502047297717 1104 42-43 0.473933094864897 1104 44-45 0.45176434246918973 1104 46-47 0.5148231976130546 1104 48-49 0.42050877076665927 1104 50-51 0.33697767848465787 1104 52-53 0.45916984926586935 1104 54-55 0.6433028281528195 1104 56-57 0.5954488494051233 1104 58-59 0.4569950098972484 1104 60-61 0.5012120744258795 1104 62-63 0.29317473395716576 1104 64-65 0.4318648374483516 1104 66-67 0.5730448830407724 1104 68-69 0.46900209441818674 1104 70-71 0.4416166346229353 1104 72-73 0.5543578230508857 1104 74-75 0.47581440545287634 1104 76-77 0.5809895892843819 1104 78-79 0.5717492364073529 1104 80-81 0.7611698452264655 1104 82-83 0.623096040766633 1104 84-85 0.7452995845957915 1104 86-87 0.7738423098049196 1104 88-89 0.627675917130631 1104 90-91 0.6032553933031686 1104 92-93 0.719300313144867 1104 94-95 0.7327708252903307 1104 96-97 0.689574191253012 1104 98-99 0.8124827212166927 1104 100-101 1.0172249226223897 1105 1 0.10450150773448286 1105 2 0.1180088529911103 1105 3 0.13267610656583528 1105 4 0.13846116895314253 1105 5 0.11437642612554555 1105 6 0.15794309081901758 1105 7 0.1666371407364693 1105 8 0.16936344716054919 1105 9 0.1423313756746225 1105 10-11 0.17135785039234008 1105 12-13 0.06381324215403339 1105 14-15 0.2831562398128895 1105 16-17 0.14440551533918722 1105 18-19 0.30019670598522197 1105 20-21 0.2500446813109818 1105 22-23 0.20471643810375895 1105 24-25 0.33352504268177796 1105 26-27 0.37593285708972957 1105 28-29 0.2529440842459678 1105 30-31 0.1600890078838475 1105 32-33 0.17856642083592789 1105 34-35 0.17965115140066956 1105 36-37 0.24100132224257464 1105 38-39 0.3747984123087562 1105 40-41 0.2181466945277677 1105 42-43 0.48136706222617676 1105 44-45 0.505824024595654 1105 46-47 0.4042089600224372 1105 48-49 0.32334357967825156 1105 50-51 0.28273196298269454 1105 52-53 0.18981265699863314 1105 54-55 0.33581421732481687 1105 56-57 0.36391731787359305 1105 58-59 0.24766509843195195 1105 60-61 0.4349436706290817 1105 62-63 0.34266523991064446 1105 64-65 0.46387055966307145 1105 66-67 0.37101499081324363 1105 68-69 0.4109072890267029 1105 70-71 0.3195517255521203 1105 72-73 0.4299559336558083 1105 74-75 0.28670779543531566 1105 76-77 0.33311792161489606 1105 78-79 0.4015270605740895 1105 80-81 0.47459658409617944 1105 82-83 0.5949240591530334 1105 84-85 0.7584496269598269 1105 86-87 0.8443371913288757 1105 88-89 0.80063916888761 1105 90-91 0.9180180319072413 1105 92-93 0.859455074903444 1105 94-95 1.05479725881154 1105 96-97 0.9016059867923047 1105 98-99 0.8760086164895249 1105 100-101 1.1752861642365602 1106 1 0.02557780597128101 1106 2 -0.07678796494535334 1106 3 -0.11274034335164274 1106 4 -0.06719951150507342 1106 5 -0.08163700557521025 1106 6 -0.10800516260154325 1106 7 -0.0789179248311811 1106 8 -0.09211573089982039 1106 9 -0.056635704109694984 1106 10-11 -0.421790197796966 1106 12-13 -0.35927587547168827 1106 14-15 -0.2596920158771425 1106 16-17 -0.24070424912100918 1106 18-19 -0.07271832996362093 1106 20-21 -0.13628603560560038 1106 22-23 -0.18469519519799604 1106 24-25 -0.0611039234946702 1106 26-27 -0.09452053845971164 1106 28-29 0.09612239203145378 1106 30-31 0.1047197317868438 1106 32-33 0.22257777975433868 1106 34-35 0.06189114915790128 1106 36-37 0.1664768317719023 1106 38-39 0.0530724894911927 1106 40-41 0.05938961189102798 1106 42-43 0.018560778376034648 1106 44-45 -0.0028365610788299023 1106 46-47 0.4166680150063513 1106 48-49 0.516075958914648 1106 50-51 0.2783721336646181 1106 52-53 0.15856873394879045 1106 54-55 0.2810864001675313 1106 56-57 0.3533884246316674 1106 58-59 0.19900866900199787 1106 60-61 0.10791853602950141 1106 62-63 0.3385711832958336 1106 64-65 0.4411085571720008 1106 66-67 0.5301208375457236 1106 68-69 0.3044248825344127 1106 70-71 0.5065079706210334 1106 72-73 0.5812039640008457 1106 74-75 0.35800396523793054 1106 76-77 0.48183070954274143 1106 78-79 0.570194939701242 1106 80-81 0.5175207112737041 1106 82-83 0.4543846996507881 1106 84-85 0.39612945462252824 1106 86-87 0.7895478537425866 1106 88-89 0.7266036156420697 1106 90-91 0.7702505226146563 1106 92-93 0.9738895252757054 1106 94-95 1.0337881991774367 1106 96-97 0.868639936724243 1106 98-99 0.9847222232064432 1106 100-101 0.981525069978364 1107 1 0.09425461635106913 1107 2 -0.022582933873547262 1107 3 0.06498769341043342 1107 4 -0.024111979384585425 1107 5 -0.03645809235606379 1107 6 -0.0018379298305291059 1107 7 0.05701088650910435 1107 8 0.03825084366902587 1107 9 0.05318272734452023 1107 10-11 0.04022144379273129 1107 12-13 0.13077462057122347 1107 14-15 0.17273315921001853 1107 16-17 0.19609997309745353 1107 18-19 0.07338416863398578 1107 20-21 -0.07242318795957203 1107 22-23 0.15273380096897426 1107 24-25 0.2868123674573937 1107 26-27 0.15628701960626046 1107 28-29 0.1539337851264051 1107 30-31 0.0815892461460237 1107 32-33 0.1504124281303021 1107 34-35 0.09520777668382152 1107 36-37 0.18547860830000218 1107 38-39 0.22962730031536438 1107 40-41 0.3658342341322083 1107 42-43 0.3407074480583816 1107 44-45 0.16241866413571415 1107 46-47 0.3595431209952338 1107 48-49 0.37661551331795806 1107 50-51 0.2753033133769236 1107 52-53 0.1642826680867202 1107 54-55 0.1706295444927619 1107 56-57 0.2804992100404249 1107 58-59 0.22314523947575537 1107 60-61 0.2692525462303408 1107 62-63 0.2003028946712888 1107 64-65 0.3355545902734107 1107 66-67 0.4960753652014702 1107 68-69 0.3158823928244274 1107 70-71 0.34591401440352954 1107 72-73 0.3623871137861805 1107 74-75 0.22659593469320427 1107 76-77 0.318149002003306 1107 78-79 0.40221215100048013 1107 80-81 0.6487474024377953 1107 82-83 0.4765880627988395 1107 84-85 0.5669005853931317 1107 86-87 0.5918523086134897 1107 88-89 0.6077261861178265 1107 90-91 0.7305058125932611 1107 92-93 0.8364385093268076 1107 94-95 0.7906539372934844 1107 96-97 0.8428703154545261 1107 98-99 0.7656846769873162 1107 100-101 0.8914223143172393 1108 1 -0.028667052011087435 1108 2 -0.09311037089108254 1108 3 -0.17270508380218175 1108 4 -0.10937868993452327 1108 5 -0.16434415542829584 1108 6 0.03508984229561918 1108 7 -0.015057810756168521 1108 8 0.017915495694637684 1108 9 0.059519391817019596 1108 10-11 -0.03692726065344232 1108 12-13 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66-67 -1.2686108525735023 2316 68-69 -1.2574556069636813 2316 70-71 -1.4228902382762527 2316 72-73 -1.442866145103313 2316 74-75 -1.5061350111022058 2316 76-77 -1.7122721001097645 2316 78-79 -1.81672078062687 2316 80-81 -1.8867455119912186 2316 82-83 -1.9499463899350502 2316 84-85 -1.9198522203942296 2316 86-87 -2.2318775910868744 2316 88-89 -2.425221950403234 2316 90-91 -2.503037476917914 2316 92-93 -2.4572120434989344 2316 94-95 -2.633420340597496 2316 96-97 -2.973109017739599 2316 98-99 -3.289133480424784 2316 100-101 -3.538465145605599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5943.0 3 1200.0 4 1080.0 5 1281.0 6 1424.0 7 1785.0 8 2192.0 9 2830.0 10 3622.0 11 4751.0 12 7138.0 13 9735.0 14 10940.0 15 14603.0 16 19668.0 17 26634.0 18 21862.0 19 12923.0 20 11939.0 21 12198.0 22 12953.0 23 14066.0 24 15833.0 25 17616.0 26 20350.0 27 23812.0 28 28199.0 29 33836.0 30 41480.0 31 52232.0 32 66340.0 33 87064.0 34 119593.0 35 169523.0 36 250456.0 37 353009.0 38 293177.0 39 40745.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46192252082394 18.75247081330189 15.222089831169155 21.56351683470501 2 15.028972658918516 20.444295337623778 41.20993184074212 23.31680016271558 3 18.415563600187912 26.16221294940172 30.693950866333214 24.728272584077153 4 11.98547269027161 16.742995287633438 38.15684895997418 33.114683062120776 5 12.791580097024328 38.067428424247346 34.02544829671292 15.1155431820154 6 30.17091623541291 36.89841964760644 17.99605505686805 14.934609060112603 7 27.466301710016317 34.32823785806677 20.79800024963742 17.4074601822795 8 26.764346386691727 33.824824928212145 20.01106850265362 19.399760182442506 9 27.44939391998021 16.04885974775937 20.49731257027855 36.00443376198187 10-11 24.525423176418013 27.142700893831233 27.173828382856513 21.15804754689424 12-13 26.084015873353415 24.739961849379892 28.17876495721657 20.99725732005012 14-15 22.86881635504792 25.309756996751027 27.614359546497035 24.20706710170402 16-17 21.07649609234763 29.022534588937067 27.827897600176744 22.073071718538564 18-19 20.95531970005838 26.523139324175876 31.830026085001865 20.691514890763877 20-21 22.091624296589526 25.539595461834068 31.82291245278497 20.545867788791437 22-23 21.674188677661395 25.61523643525161 31.775028911694243 20.935545975392753 24-25 21.27856085144616 25.83109520205583 31.948973091895873 20.941370854602138 26-27 21.072526321951177 26.0722060296679 31.80557595731498 21.049691691065945 28-29 20.78774665409262 26.159728157538286 32.11590584927987 20.93661933908923 30-31 20.932843457167642 25.97233910161322 32.0648125721012 21.030004869117942 32-33 20.85846299405173 26.048721186903368 31.788598509880966 21.304217309163935 34-35 21.249192491963587 26.181129818016206 31.51384522415097 21.055832465869234 36-37 21.316928721839723 25.908228132968038 31.5093019415701 21.265541203622142 38-39 21.1096366100392 26.055980233954166 31.555593108391843 21.278790047614788 40-41 21.171690195951744 26.142398355228007 31.49053043951981 21.19538100930044 42-43 21.0693927885931 26.267604447602167 31.117033834724534 21.5459689290802 44-45 21.210309526597285 26.532610640680954 30.817099626871443 21.439980205850322 46-47 21.366255066754963 26.824459826894966 30.149504826361984 21.65978027998809 48-49 21.521756421317527 26.99383690499731 29.684184188054484 21.800222485630677 50-51 21.61778868261573 27.241820151739148 29.40528095991542 21.7351102057297 52-53 21.764422192501403 27.321690285877803 29.27718750466114 21.636700016959647 54-55 21.3534283513629 27.24213800784701 29.327199111184022 22.07723452960607 56-57 21.523241160937047 27.572917506496836 29.045045452989203 21.858795879576913 58-59 21.701953767823024 27.099803408325325 29.239670653722538 21.95857217012911 60-61 21.670286355787553 27.072762101661635 29.64417710467942 21.61277443787139 62-63 21.644483862541534 27.12922613206302 29.604890028479332 21.621399976916113 64-65 21.464405641555476 27.215132206354898 29.523407728341244 21.797054423748385 66-67 21.353338078574794 27.434223277783822 29.27053784863858 21.94190079500281 68-69 21.353354064173207 27.380252618803148 29.462235976520017 21.80415734050363 70-71 21.356923438006945 27.467358814880267 29.206933447464156 21.968784299648632 72-73 21.52386479950012 27.463605908098277 29.009245174812854 22.003284117588752 74-75 21.270911062954642 27.69342111948327 28.96746897994438 22.068198837617715 76-77 21.404729742416695 27.54233871635794 29.00780558816935 22.04512595305602 78-79 21.370150405191936 27.55120157653336 28.955160084569957 22.12348793370475 80-81 21.274107094535267 27.923307933513513 28.7135777297443 22.08900724220692 82-83 21.30576174819452 27.695935774659088 28.81503627530132 22.183266201845075 84-85 21.32356920168174 27.469903069163582 28.727728933804975 22.478798795349704 86-87 21.362372362489428 27.429889099209937 28.653005983474216 22.554732554826426 88-89 21.435318030806236 27.14473420102934 28.762656584378103 22.657291183786317 90-91 21.16882722440809 27.2429626561745 29.079340098018914 22.508870021398494 92-93 21.284087956736723 26.865386479038587 29.178636617018007 22.671888947206686 94-95 20.97497379874565 26.95112375495726 29.14181366794369 22.932088778353403 96-97 21.050090088498212 26.857140017349558 29.03146719735041 23.061302696801818 98-99 20.904875650628394 26.951139674809365 28.998525826747784 23.14545884781446 100-101 20.84117186158397 26.782863702649262 29.49617704370931 22.87978739205746 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2697.0 1 1607.0 2 617.5 3 745.5 4 670.5 5 705.0 6 1192.0 7 2414.5 8 4054.5 9 4834.0 10 4342.5 11 3844.5 12 3930.0 13 4145.0 14 4394.0 15 4637.0 16 4743.5 17 4845.0 18 5128.0 19 5446.0 20 5815.0 21 6495.5 22 7465.0 23 8529.5 24 9914.0 25 11901.5 26 14484.5 27 17240.5 28 19298.0 29 21837.0 30 25276.0 31 28322.5 32 31234.5 33 34032.0 34 38024.5 35 40982.5 36 43711.5 37 47873.0 38 50358.0 39 50940.5 40 51489.5 41 54328.0 42 57540.5 43 59962.0 44 61807.0 45 63441.5 46 63004.0 47 63199.0 48 68050.5 49 67854.5 50 63173.5 51 59948.5 52 57436.5 53 56545.0 54 54597.5 55 50400.0 56 45098.0 57 39911.5 58 36476.0 59 32996.5 60 28769.5 61 24397.5 62 19715.5 63 14883.5 64 10779.0 65 8226.5 66 6048.5 67 4592.5 68 3365.5 69 2179.0 70 1630.5 71 1215.0 72 897.0 73 773.5 74 580.0 75 407.5 76 292.0 77 184.0 78 110.5 79 79.0 80 55.5 81 34.0 82 23.5 83 18.0 84 19.0 85 10.5 86 9.0 87 10.5 88 12.0 89 9.5 90 8.0 91 8.5 92 5.0 93 3.5 94 4.5 95 5.0 96 8.0 97 9.5 98 9.0 99 6.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2988913677063919 2 0.2615712909935134 3 0.2583740022943303 4 0.2621225476657863 5 0.26460320269101456 6 0.19944466402835223 7 0.18803365091230223 8 0.1926090812921677 9 0.18660038356439257 10-11 0.20616999543008216 12-13 0.19743257717455598 14-15 0.1909828741089625 16-17 0.19222320162157663 18-19 0.1884195305828933 20-21 0.18221789301982264 22-23 0.18472611087866456 24-25 0.17576818995422916 26-27 0.1759060041222974 28-29 0.1890534757560072 30-31 0.20107087121155742 32-33 0.19351865480141806 34-35 0.20305539523174004 36-37 0.1811429425088904 38-39 0.17943404682484426 40-41 0.17687070329877505 42-43 0.18238327002150453 44-45 0.1874823942400293 46-47 0.19073480860643965 48-49 0.19751526567539693 50-51 0.19996835786701153 52-53 0.21267482416290295 54-55 0.1950621734837823 56-57 0.17392148010211478 58-59 0.17513424478111528 60-61 0.17133057374243194 62-63 0.17915841848870778 64-65 0.1718818304147049 66-67 0.17088956840461356 68-69 0.1679954708751806 70-71 0.17913085565509412 72-73 0.1764296979609567 74-75 0.18042630883493554 76-77 0.1809775655072085 78-79 0.17797321664332094 80-81 0.1798750521626626 82-83 0.17436248543993316 84-85 0.16840891337938532 86-87 0.16807815937602152 88-89 0.16937361255586297 90-91 0.17430735977270587 92-93 0.16970436655922674 94-95 0.16840891337938532 96-97 0.1675820283709759 98-99 0.17593356695591106 100-101 0.1655975043507933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1814037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.11814902928747 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89391865869787 35.8041436000668 2 14.226413719673248 13.975502184704208 3 5.154215736354979 7.594966100021476 4 2.44629280209505 4.806294976903117 5 1.3808194597241057 3.3911648002634416 6 0.8414761929572946 2.479905183016431 7 0.5751772855897787 1.9776150542301851 8 0.40552216206512703 1.5934798392795018 9 0.2911587076411902 1.2871059113785248 >10 1.602380741433904 14.404864956980102 >50 0.11840592215094732 4.005891947613953 >100 0.06063260750593731 5.4221108351942595 >500 0.002577440454437524 0.8784326549583763 >1k 6.723757707228324E-4 0.5436055454833588 >5k 1.1206262845380539E-4 0.42603350769959475 >10k+ 2.2412525690761079E-4 1.4088829022065668 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14969 0.8251761127253744 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10627 0.5858204656244608 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7740 0.42667266433926104 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2409 0.13279773235055295 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2356 0.1298760719875063 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1822 0.10043896568813093 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.51256672272947E-5 0.0 22-23 0.0 0.0 0.0 1.653770016818841E-4 0.0 24-25 0.0 0.0 0.0 4.134425042047103E-4 0.0 26-27 0.0 0.0 0.0 7.993221747957732E-4 0.0 28-29 0.0 0.0 0.0 0.00187427268572802 0.0 30-31 0.0 0.0 0.0 0.004630556047092755 0.0 32-33 0.0 0.0 0.0 0.008048347415185026 0.0 34-35 0.0 0.0 0.0 0.01154882728411824 0.0 36-37 0.0 5.51256672272947E-5 0.0 0.013808979640437324 0.0 38-39 0.0 5.51256672272947E-5 0.0 0.015573000991710753 0.0 40-41 0.0 5.51256672272947E-5 0.0 0.018439535687530078 0.0 42-43 0.0 5.51256672272947E-5 0.0 0.021995141223690586 0.0 44-45 0.0 5.51256672272947E-5 0.0 0.0260193149312831 0.0 46-47 0.0 5.51256672272947E-5 0.0 0.03183507282376269 0.0 48-49 0.0 5.51256672272947E-5 0.0 0.03935972640028841 0.0 50-51 0.0 5.51256672272947E-5 0.0 0.049006718165064994 0.0 52-53 0.0 5.51256672272947E-5 0.0 0.07866432713334955 0.0 54-55 0.0 5.51256672272947E-5 0.0 0.11204291863947649 0.0 56-57 0.0 5.51256672272947E-5 0.0 0.15145777070699218 0.0 58-59 0.0 5.51256672272947E-5 0.0 0.21091080281162955 0.0 60-61 0.0 5.51256672272947E-5 0.0 0.30740828329300895 0.0 62-63 0.0 5.51256672272947E-5 0.0 0.3668888782312599 0.0 64-65 0.0 5.51256672272947E-5 0.0 0.43149616022164927 0.0 66-67 0.0 5.51256672272947E-5 0.0 0.5656169085856573 0.0 68-69 0.0 5.51256672272947E-5 0.0 0.7263909170540623 0.0 70-71 0.0 5.51256672272947E-5 0.0 0.8554401040331592 0.0 72-73 0.0 5.51256672272947E-5 0.0 0.9970855059736929 0.0 74-75 0.0 5.51256672272947E-5 0.0 1.268606979901733 0.0 76-77 0.0 5.51256672272947E-5 0.0 1.458514903499763 0.0 78-79 0.0 5.51256672272947E-5 0.0 1.6194818518034637 0.0 80-81 0.0 5.51256672272947E-5 0.0 1.8485014362992596 0.0 82-83 0.0 5.51256672272947E-5 0.0 2.224375798288569 0.0 84-85 0.0 5.51256672272947E-5 0.0 2.596529177740035 0.0 86-87 0.0 5.51256672272947E-5 0.0 3.069700342385519 0.0 88-89 0.0 5.51256672272947E-5 0.0 3.685205979811878 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7120 0.0 44.54569 1 GTATCAA 12655 0.0 41.294804 1 TATCAAC 16110 0.0 32.339314 2 ATCAACG 16215 0.0 32.099697 3 TCAACGC 16375 0.0 31.788687 4 CAACGCA 16550 0.0 31.450813 5 AACGCAG 16995 0.0 30.605299 6 ACGCAGA 18985 0.0 27.247145 7 CGCAGAG 19025 0.0 27.065018 8 GTACATG 11960 0.0 25.723671 1 GCAGAGT 19825 0.0 25.517616 9 TACATGG 11995 0.0 24.84752 2 GAGTACT 11315 0.0 23.82336 12-13 ACATGGG 12135 0.0 23.620075 3 AGTACTT 11595 0.0 22.858892 12-13 GTACTTT 11975 0.0 22.56984 14-15 CATGGGA 7945 0.0 21.121296 4 AGAGTAC 18695 0.0 20.974678 10-11 CATGGGG 4005 0.0 20.415768 4 CAGAGTA 19780 0.0 20.304441 10-11 >>END_MODULE