FastQCFastQC Report
Thu 26 May 2016
SRR937422_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937422_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814037
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159550.8795300206114869No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125460.6916066210336393No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87660.4832315989144654No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.1549031249086981No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21700.1196226978832295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.045.2702521
GTATCAA133950.039.551541
TATCAAC175150.030.2396132
ATCAACG176800.029.9036663
TCAACGC178650.029.6205864
CAACGCA181350.029.1272015
AACGCAG186150.028.3993076
ACGCAGA211550.024.8350817
CGCAGAG211700.024.7501688
GCAGAGT219250.023.442899
GTACATG121050.023.3944441
AGTACTT129700.022.81768212-13
GAGTACT126650.022.74830612-13
TACATGG121850.022.2598422
GTACTTT133800.021.29956414-15
ACATGGG121350.021.255513
AGAGTAC206850.020.8867410-11
TACTTTT143350.020.59297214-15
ATGGGAG30650.019.9926685
CATTCCG3203.965397E-1019.29829
Produced by FastQC (version 0.11.5)