##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937422_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1814037 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21683129947184 33.0 31.0 34.0 31.0 34.0 2 32.42913016658425 34.0 31.0 34.0 31.0 34.0 3 32.49367846411071 34.0 31.0 34.0 31.0 34.0 4 36.119666798417015 37.0 35.0 37.0 35.0 37.0 5 35.994885991851326 37.0 35.0 37.0 35.0 37.0 6 36.09167233082898 37.0 35.0 37.0 35.0 37.0 7 36.0536940536494 37.0 35.0 37.0 35.0 37.0 8 36.03411892921699 37.0 35.0 37.0 35.0 37.0 9 37.730890274013156 39.0 37.0 39.0 35.0 39.0 10-11 37.65983659649721 39.0 37.0 39.0 35.0 39.0 12-13 37.56913613118145 39.0 37.0 39.0 35.0 39.0 14-15 38.75767445757722 40.0 38.0 41.0 35.0 41.0 16-17 38.7518057790442 40.0 38.0 41.0 35.0 41.0 18-19 38.805204083488924 40.0 38.0 41.0 35.0 41.0 20-21 38.74068141939773 40.0 38.0 41.0 34.5 41.0 22-23 38.616592991212414 40.0 38.0 41.0 34.0 41.0 24-25 38.47045760367622 40.0 38.0 41.0 34.0 41.0 26-27 38.25007841626163 40.0 38.0 41.0 34.0 41.0 28-29 38.20503909236692 40.0 38.0 41.0 34.0 41.0 30-31 38.2460211671537 40.0 37.5 41.0 34.0 41.0 32-33 38.132818680104094 40.0 37.0 41.0 34.0 41.0 34-35 37.98975820228584 40.0 37.0 41.0 33.5 41.0 36-37 37.81130842424934 40.0 37.0 41.0 33.0 41.0 38-39 37.641516132250885 40.0 37.0 41.0 33.0 41.0 40-41 37.43329601325662 40.0 36.0 41.0 33.0 41.0 42-43 37.1674770139749 40.0 36.0 41.0 32.5 41.0 44-45 36.7720840313621 40.0 36.0 41.0 31.0 41.0 46-47 36.27825507417985 39.5 35.5 41.0 30.5 41.0 48-49 35.830024415158015 39.0 35.0 41.0 28.5 41.0 50-51 35.55762478935104 39.0 35.0 41.0 27.5 41.0 52-53 35.29695838618507 39.0 35.0 40.5 27.0 41.0 54-55 35.07544030248556 39.0 35.0 40.0 26.0 41.0 56-57 34.83223054436045 38.0 35.0 40.0 26.0 41.0 58-59 34.5702455352344 38.0 34.0 40.0 25.0 41.0 60-61 34.24459534177087 38.0 34.0 40.0 24.5 41.0 62-63 33.87125620921734 37.0 34.0 40.0 23.0 41.0 64-65 33.52761768365254 37.0 33.5 39.5 22.0 41.0 66-67 33.14251225305768 36.0 33.0 39.0 21.5 41.0 68-69 32.718825470483786 36.0 33.0 39.0 20.0 40.0 70-71 32.34367573539018 35.0 32.0 38.5 18.5 40.0 72-73 31.920482603166306 35.0 32.0 37.5 17.5 40.0 74-75 31.49513460861052 35.0 31.5 37.0 14.0 39.0 76-77 30.196898133830786 34.0 29.5 35.5 11.0 38.5 78-79 30.78926697746518 34.5 31.0 36.0 9.0 38.5 80-81 30.622008536760827 35.0 31.0 36.0 7.0 37.5 82-83 30.311882833701848 34.0 31.0 35.0 4.0 37.0 84-85 29.998771800134175 34.0 31.0 35.0 2.0 36.5 86-87 29.667700272927178 34.0 30.5 35.0 2.0 36.0 88-89 29.32861347370533 34.0 30.0 35.0 2.0 36.0 90-91 28.91603671810443 34.0 29.5 35.0 2.0 35.0 92-93 28.529942608667852 34.0 29.0 35.0 2.0 35.0 94-95 28.15935424690897 34.0 29.0 35.0 2.0 35.0 96-97 27.77602165777214 34.0 29.0 35.0 2.0 35.0 98-99 27.19536619153854 33.0 27.0 35.0 2.0 35.0 100-101 25.690980393453938 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09849561142827667 1101 2 0.12404367626805168 1101 3 0.11855594864788088 1101 4 0.06950886835898729 1101 5 -0.0630187521121428 1101 6 0.010419136178263955 1101 7 0.05147403673713313 1101 8 0.06842867564491684 1101 9 0.12905416099402345 1101 10-11 0.12135257245161313 1101 12-13 0.1086725271829252 1101 14-15 0.16535784495575 1101 16-17 0.1394941344720806 1101 18-19 0.13241620979103175 1101 20-21 0.04624325504342863 1101 22-23 0.027316587186540175 1101 24-25 0.17494997346019403 1101 26-27 0.03493084903410448 1101 28-29 -0.011544743295551996 1101 30-31 0.09211611542587406 1101 32-33 0.10709347469732933 1101 34-35 0.16010412343297276 1101 36-37 0.10845502286090891 1101 38-39 0.08510185751993049 1101 40-41 0.038650517760409286 1101 42-43 0.010558217802923764 1101 44-45 0.061288396757149144 1101 46-47 0.07601465326078483 1101 48-49 0.08355557252779988 1101 50-51 0.11049036533524514 1101 52-53 0.22409424914285125 1101 54-55 0.059909654250674294 1101 56-57 0.13372466150681817 1101 58-59 0.2830019116783902 1101 60-61 0.23790582984799613 1101 62-63 0.06750941279850053 1101 64-65 0.09996660630079646 1101 66-67 -0.01193076554090311 1101 68-69 -0.13798979331414074 1101 70-71 -0.133397878830273 1101 72-73 -0.12574931029620018 1101 74-75 0.003041641285825847 1101 76-77 0.08705440286543364 1101 78-79 0.1355539194113149 1101 80-81 0.006250299333704135 1101 82-83 0.05953560968087146 1101 84-85 0.07132738630624758 1101 86-87 -0.002015237181730356 1101 88-89 0.08058703556065083 1101 90-91 0.3047837325572722 1101 92-93 0.16163858209338855 1101 94-95 0.020361107348236374 1101 96-97 -0.08281918954929424 1101 98-99 -0.14232943915428464 1101 100-101 0.12098800327579085 1102 1 0.11821006644345289 1102 2 0.06149477133326542 1102 3 0.029445222278106087 1102 4 0.04542708444593302 1102 5 -0.0260590708169417 1102 6 -0.030903820719771602 1102 7 0.059912417966899056 1102 8 0.09642343338332893 1102 9 0.13638039527061352 1102 10-11 0.09960365284775463 1102 12-13 0.0680818655886668 1102 14-15 0.11427214092735483 1102 16-17 0.01631459473338026 1102 18-19 0.0651234055478156 1102 20-21 0.019146302156222816 1102 22-23 0.09546492620413716 1102 24-25 0.019825257432110277 1102 26-27 0.04183042575429852 1102 28-29 0.04831904668971987 1102 30-31 0.026291858578225913 1102 32-33 0.035677214807776636 1102 34-35 0.17581112424556267 1102 36-37 0.03402475208149269 1102 38-39 0.09922430949320926 1102 40-41 0.19222426939378323 1102 42-43 0.15544797782855113 1102 44-45 0.18417638877409104 1102 46-47 -0.0013731717990026482 1102 48-49 0.05620808740435734 1102 50-51 0.0708367869512685 1102 52-53 0.17588773569072913 1102 54-55 0.13568262726538194 1102 56-57 0.25600157104305765 1102 58-59 0.25586133761600394 1102 60-61 0.223467896410817 1102 62-63 0.328260681383334 1102 64-65 0.23777477735757202 1102 66-67 0.21800561702838905 1102 68-69 0.14582983891688173 1102 70-71 0.07082430350238411 1102 72-73 0.10133702681828183 1102 74-75 0.06276916335215788 1102 76-77 0.09349952800609174 1102 78-79 0.04979189029321418 1102 80-81 0.011238264907721174 1102 82-83 0.024574588868166103 1102 84-85 0.030549115055883647 1102 86-87 0.09648311351321581 1102 88-89 0.22208978504578525 1102 90-91 0.01682394896462469 1102 92-93 0.09201494018849488 1102 94-95 0.006056353605210063 1102 96-97 0.20454263651802407 1102 98-99 0.23023795652644807 1102 100-101 0.4507763440691157 1103 1 0.12261025368546541 1103 2 0.08855002596416028 1103 3 0.013191643098444672 1103 4 0.05305598050838256 1103 5 -0.03463147207413897 1103 6 0.007386562457632806 1103 7 0.052760137930668805 1103 8 0.012874589359576305 1103 9 0.04586408150650101 1103 10-11 0.05282385066772832 1103 12-13 0.10670174606990912 1103 14-15 0.04139943509792232 1103 16-17 0.004362414397434122 1103 18-19 -0.024338760015467642 1103 20-21 -0.04860156553419159 1103 22-23 0.06652357307665113 1103 24-25 0.011510313939076866 1103 26-27 0.03936314751145176 1103 28-29 0.001558698225039734 1103 30-31 0.0030881356234502277 1103 32-33 0.10756021286848494 1103 34-35 0.03606931152405224 1103 36-37 0.14154543685227594 1103 38-39 0.050638655392425846 1103 40-41 -0.06394225440966039 1103 42-43 -0.061444838279122393 1103 44-45 -0.08446908550141075 1103 46-47 -0.11627167811842298 1103 48-49 -0.27566287366997244 1103 50-51 -0.1849710589753002 1103 52-53 -0.05850126566494396 1103 54-55 0.03258857463092113 1103 56-57 -0.05665063908138279 1103 58-59 -0.03636355949208081 1103 60-61 -0.12944894503532112 1103 62-63 -0.0354310866554286 1103 64-65 -0.06588069734348778 1103 66-67 -0.07607224646732647 1103 68-69 0.019469446681355862 1103 70-71 -0.10732127108133938 1103 72-73 -0.12447349399415586 1103 74-75 -0.013433775374718238 1103 76-77 -0.3324310587058079 1103 78-79 0.15613349753486006 1103 80-81 0.09145474828391187 1103 82-83 -0.019047328173893874 1103 84-85 -0.1487320701617243 1103 86-87 -0.047114486384650434 1103 88-89 0.04163864122811489 1103 90-91 0.13704547814824153 1103 92-93 0.106395491336567 1103 94-95 -0.15593564605435262 1103 96-97 -0.12427317935255999 1103 98-99 -0.016864047814163285 1103 100-101 0.2501206930687694 1104 1 0.06608944716465714 1104 2 0.07230316686015925 1104 3 0.02080333642442156 1104 4 0.013278447873702248 1104 5 -0.02202192375030876 1104 6 -0.022444288801793277 1104 7 -0.01812299009531415 1104 8 -0.006703151646739514 1104 9 -0.005082783726081175 1104 10-11 0.08722049220722283 1104 12-13 0.06492629715159381 1104 14-15 0.0340466741737373 1104 16-17 -0.037662774056322235 1104 18-19 0.011127318307750045 1104 20-21 -0.040694168341083525 1104 22-23 -0.0612465439791805 1104 24-25 -0.08500712186407355 1104 26-27 -0.14165205136088588 1104 28-29 -0.1562935417700544 1104 30-31 -0.08451834841161343 1104 32-33 -0.07720107903566742 1104 34-35 -0.024937613768592826 1104 36-37 0.07612133928532216 1104 38-39 -0.008793225468025412 1104 40-41 0.1121225859409023 1104 42-43 0.21075133748848174 1104 44-45 0.31453348850116924 1104 46-47 0.2553177684710306 1104 48-49 0.050489298910775915 1104 50-51 0.23886144700006184 1104 52-53 0.2779775600995862 1104 54-55 0.14299489932295728 1104 56-57 0.14013798627867402 1104 58-59 0.1209049334906993 1104 60-61 0.028623923751830205 1104 62-63 0.015048474538417622 1104 64-65 0.17524971878692952 1104 66-67 -0.011545985512491086 1104 68-69 -0.06900423107803988 1104 70-71 -0.07216775021353072 1104 72-73 0.05827915661563665 1104 74-75 0.019252993026334764 1104 76-77 0.05021280965235064 1104 78-79 0.0644683630125833 1104 80-81 0.11032770287114957 1104 82-83 0.0696148033453774 1104 84-85 0.04957491028811134 1104 86-87 -0.02066660993677516 1104 88-89 -0.09188927444265715 1104 90-91 0.10378262345681222 1104 92-93 0.048393101191322785 1104 94-95 -0.1704036855223876 1104 96-97 -0.03078632515141777 1104 98-99 0.12531741936730612 1104 100-101 0.3713056535394479 1105 1 0.038645533464496395 1105 2 0.024890238256858765 1105 3 0.01814115584311793 1105 4 -0.0052743393524110616 1105 5 -0.09608756140067953 1105 6 -0.013819012002954878 1105 7 0.017032201443335282 1105 8 0.006115550744887344 1105 9 -0.04547597109791468 1105 10-11 -0.029091708225834623 1105 12-13 -0.047956258279803876 1105 14-15 0.09002256146493437 1105 16-17 0.011576822195998204 1105 18-19 -0.01479578789703595 1105 20-21 -0.02581548911664555 1105 22-23 -0.03430639392453827 1105 24-25 -0.02901445268421554 1105 26-27 0.07549566200444957 1105 28-29 -0.10855101278036727 1105 30-31 -0.07946689759687331 1105 32-33 -0.06453791609990134 1105 34-35 -0.13483053713266457 1105 36-37 -0.05903108938142054 1105 38-39 -0.07884021475626923 1105 40-41 0.017264597557236527 1105 42-43 -0.14450108667579542 1105 44-45 0.033412396941635336 1105 46-47 0.015843594160415364 1105 48-49 -0.08377225705682179 1105 50-51 0.022703251094021937 1105 52-53 -0.04326370615530095 1105 54-55 -0.05692855889218151 1105 56-57 0.028174955551449443 1105 58-59 0.025061725448495054 1105 60-61 -0.1173994849362785 1105 62-63 -0.05886766104286778 1105 64-65 -0.0645703261557884 1105 66-67 -0.1593069336482671 1105 68-69 -0.08956746809970184 1105 70-71 -0.11662501984118023 1105 72-73 -0.06688886692812446 1105 74-75 -0.08864984393147779 1105 76-77 -0.12959530377684558 1105 78-79 -0.2028703978291304 1105 80-81 -0.06441657798398737 1105 82-83 -0.09777880562024066 1105 84-85 -0.27662745063710403 1105 86-87 -0.1443084025699335 1105 88-89 -0.1652906972193655 1105 90-91 -0.15449248490915934 1105 92-93 -0.021408197153821362 1105 94-95 -0.054558205785497194 1105 96-97 -0.13551544090961087 1105 98-99 -0.06735841184782387 1105 100-101 -0.12069931144371182 1106 1 -0.20273682278795846 1106 2 -0.06286726311927993 1106 3 -0.13216804828055473 1106 4 -0.062487670840503995 1106 5 -0.1319834523700152 1106 6 -0.08208154603943996 1106 7 -0.060959460257940634 1106 8 -0.13745510830467111 1106 9 -0.18496736958520188 1106 10-11 -0.23430539214921708 1106 12-13 -0.34782440653935964 1106 14-15 -0.14491561328512859 1106 16-17 -0.05155548655334741 1106 18-19 -0.16492821812975933 1106 20-21 -0.175645990878607 1106 22-23 -0.1779134312386219 1106 24-25 -0.14541755678478552 1106 26-27 0.013837841078071733 1106 28-29 -0.028349510569420033 1106 30-31 -0.11422226878644182 1106 32-33 -0.12015049270006983 1106 34-35 -0.13980512574678272 1106 36-37 -0.008116203840565106 1106 38-39 -0.07249159572858588 1106 40-41 0.0393282931172223 1106 42-43 -0.13202623440270145 1106 44-45 0.0610395195076876 1106 46-47 0.09143785035506369 1106 48-49 -0.04258827915700891 1106 50-51 0.050564996779023375 1106 52-53 0.14150761807137258 1106 54-55 0.1414970743677344 1106 56-57 0.05895719726242987 1106 58-59 -0.020371945739171338 1106 60-61 0.0898704052970416 1106 62-63 0.023986673266037428 1106 64-65 0.07437550961203954 1106 66-67 0.17973993802122834 1106 68-69 0.22797695676994323 1106 70-71 0.20672858000502714 1106 72-73 0.23806734343124347 1106 74-75 0.18909531330940155 1106 76-77 0.1400792186666493 1106 78-79 0.19374190687966575 1106 80-81 0.1827421349329903 1106 82-83 0.19814892022425568 1106 84-85 0.10290475825557266 1106 86-87 0.21028255666371365 1106 88-89 0.22098606416725275 1106 90-91 0.140408083392753 1106 92-93 0.13593335937310513 1106 94-95 -0.04477323136597988 1106 96-97 0.2554639064709967 1106 98-99 -0.011600767183590932 1106 100-101 0.04982719663003721 1107 1 -0.06440875195001894 1107 2 -0.0077789498445426375 1107 3 -0.04064397049592827 1107 4 -0.011672291268283175 1107 5 -0.08845901190662175 1107 6 -0.02592462570023457 1107 7 -0.09541992080018247 1107 8 -0.07900205278592409 1107 9 -0.15663209499054886 1107 10-11 0.020298585559984872 1107 12-13 -0.011703893568117962 1107 14-15 -0.010525695754687092 1107 16-17 -0.05183546658608407 1107 18-19 -0.010862728707728309 1107 20-21 -0.04454957512121638 1107 22-23 -9.986816713052349E-4 1107 24-25 -0.0389230163954295 1107 26-27 0.028377871746350536 1107 28-29 -0.1470936755762935 1107 30-31 -0.056381233677349485 1107 32-33 -0.193282572533235 1107 34-35 -0.15518011466216564 1107 36-37 -0.14788427874368892 1107 38-39 -0.07098334030310838 1107 40-41 0.06696710492580138 1107 42-43 0.06650202714049414 1107 44-45 -0.02759508462943927 1107 46-47 -0.07630655676977938 1107 48-49 -0.06604333239805271 1107 50-51 -0.014728638507627068 1107 52-53 -0.11616268899996385 1107 54-55 -0.044535459112793774 1107 56-57 -0.011435611734398776 1107 58-59 -0.12263066721682492 1107 60-61 -0.03369793523838638 1107 62-63 -0.09278766780941794 1107 64-65 0.02247209375600079 1107 66-67 0.008656694278180055 1107 68-69 -0.0741431696096555 1107 70-71 -0.2079233430393188 1107 72-73 -0.06600965406686399 1107 74-75 -0.00778594825472112 1107 76-77 -0.07543491335040997 1107 78-79 0.027248691662698832 1107 80-81 0.013644906137489698 1107 82-83 -0.1034608155084733 1107 84-85 -0.12259499613916702 1107 86-87 -0.0071590535502750186 1107 88-89 0.016536623479680657 1107 90-91 0.14622165455302039 1107 92-93 0.2728216752384114 1107 94-95 0.1548460555717064 1107 96-97 -0.07046697731935225 1107 98-99 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-0.7300985403314009 2316 90-91 -0.640221694308881 2316 92-93 -0.6963943809318565 2316 94-95 -0.5021936908968243 2316 96-97 -0.6260347333169136 2316 98-99 -0.8156314415259409 2316 100-101 -0.6575115610462241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 10.0 5 45.0 6 84.0 7 216.0 8 489.0 9 940.0 10 1753.0 11 3042.0 12 4812.0 13 7013.0 14 8879.0 15 12831.0 16 18356.0 17 27204.0 18 23794.0 19 12317.0 20 9539.0 21 9387.0 22 9283.0 23 9650.0 24 10836.0 25 12809.0 26 15318.0 27 18443.0 28 23059.0 29 29269.0 30 36412.0 31 45645.0 32 59077.0 33 79084.0 34 111421.0 35 162780.0 36 255760.0 37 400646.0 38 354817.0 39 39008.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.880035120926735 19.772453015833097 15.474165602229032 20.87334626101114 2 15.638214656040644 21.43225303563268 39.89279160237636 23.036740705950322 3 18.84013391127083 26.52437629441957 30.647059569347263 23.988430224962336 4 12.608356509108148 17.681081125777617 37.27419812737902 32.43636423773522 5 12.913585256423685 38.758728748055226 33.430512384264496 14.897173611256598 6 30.411121713614442 37.439148154089466 17.302403423965444 14.847326708330646 7 27.452306650856624 34.97552696003444 20.20884910285733 17.363317286251604 8 27.146855328750185 34.3378332415491 19.36079583823263 19.15451559146809 9 27.724903075295597 16.204686012468322 20.09821188873215 35.97219902350393 10-11 24.879674449859625 27.675372663291874 26.45455963687621 20.9903932499723 12-13 26.412360273118107 25.236853138236892 27.687622609451633 20.663163979193364 14-15 23.006614528810605 25.839357190619594 27.155675435506556 23.99835284506325 16-17 21.221810800992483 29.485782263537075 27.506274679072146 21.7861322563983 18-19 21.12476206383883 26.77969082218279 31.686619401919586 20.408927712058794 20-21 22.211123587887126 25.78345976405112 31.894553418700944 20.110863229360813 22-23 21.79329787251636 25.881480975137556 31.75321564634588 20.572005506000206 24-25 21.432447964542988 26.167960518790373 31.915117053076646 20.484474463589994 26-27 21.229958021063887 26.390307634212697 31.81876565941108 20.560968685312332 28-29 20.938165747099 26.466321552007138 32.057735821076946 20.53777687981691 30-31 21.07359541704474 26.291712467186244 31.986145733302614 20.648546382466403 32-33 21.042845322339073 26.322092658529016 31.746623690696495 20.88843832843542 34-35 21.363842082603608 26.41117573676832 31.50520083107456 20.71978134955351 36-37 21.591639193389543 26.192761938782343 31.46374478338489 20.751854084443224 38-39 21.32162351973557 26.348872182109044 31.478902821005562 20.850601477149823 40-41 21.383478607921784 26.49247313724755 31.379586733670116 20.744461521160552 42-43 21.375712160789835 26.64655654338902 30.87944070224264 21.098290593578504 44-45 21.381013727944907 26.896391859702973 30.588681487753554 21.13391292459856 46-47 21.67284901024621 27.24318743222988 29.85090712041706 21.23305643710685 48-49 21.96479454388196 27.461595325784426 29.24039035587477 21.333219774458843 50-51 22.047985252777437 27.705975045154823 28.87119415733917 21.374845544728565 52-53 22.136826391537983 27.89109477430437 28.691823095021267 21.28025573913638 54-55 21.673345141251254 27.856295103131856 28.7886630757807 21.681696679836186 56-57 21.882602174046063 28.213399175430272 28.50592352857191 21.398075121951756 58-59 21.945362746184337 27.68033397334233 28.712231338170056 21.66207194230327 60-61 21.936570202261585 27.683944704545716 29.11668835861672 21.262796734575975 62-63 21.961294064013025 27.7647038070337 29.08405396361816 21.189948165335107 64-65 21.75909311662331 27.668785146058212 29.12134647749743 21.45077525982105 66-67 21.674778408599163 28.12558398753719 28.669867262905885 21.529770340957764 68-69 21.748398737181216 27.951965698604823 28.833535368903725 21.46610019531024 70-71 21.66328470698227 28.19101815453599 28.601594124044876 21.54410301443686 72-73 21.841754054630638 28.122359136004395 28.3465552246178 21.68933158474717 74-75 21.67588092194371 28.402590465354344 28.15331219815252 21.76821641454943 76-77 21.708184562938904 28.16811343980305 28.42880272012092 21.694899277137125 78-79 21.743768181134122 28.12627305837753 28.299974035810738 21.82998472467761 80-81 21.57764698294467 28.605563172085247 28.11513767359762 21.70165217137247 82-83 21.606407615313756 28.646124179933175 27.994866675229556 21.752601529523506 84-85 21.58671515520356 28.33431732649334 28.01519483891453 22.063772679388567 86-87 21.504522785367662 28.17139341700307 28.005685661317827 22.318398136311444 88-89 21.541153791240202 27.993144572023613 28.045513955889547 22.42018768084664 90-91 21.441967851891793 28.188803567087138 28.21920738379981 22.15002119722126 92-93 21.37516917080808 27.856030474174403 28.252145127191643 22.516655227825876 94-95 21.11856042627576 27.8240741506375 28.318303320163814 22.739062102922926 96-97 21.316158380451995 27.903923679616238 28.17213761351064 22.607780326421125 98-99 21.040674966855292 27.91878788212887 27.963247271935458 23.077289879080382 100-101 20.635016458009268 28.393311826694312 28.11150924948115 22.86016246581528 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1139.0 1 812.0 2 554.0 3 692.5 4 688.0 5 679.0 6 1149.0 7 2457.5 8 3973.0 9 4799.0 10 4525.0 11 4074.0 12 4221.0 13 4546.0 14 4930.5 15 5144.5 16 5275.0 17 5381.5 18 5532.0 19 5828.0 20 6160.0 21 6789.5 22 7740.5 23 8837.0 24 9934.5 25 11751.5 26 14562.0 27 17216.5 28 19343.0 29 22027.0 30 25287.0 31 28121.0 32 30894.0 33 33632.5 34 37897.5 35 40833.5 36 43625.0 37 47781.0 38 49945.0 39 50647.0 40 50891.0 41 53627.5 42 57122.5 43 59761.5 44 61778.0 45 63201.5 46 62407.0 47 62774.0 48 68240.0 49 68101.5 50 63381.5 51 60130.5 52 57595.5 53 56404.0 54 54234.5 55 50304.5 56 45139.5 57 40001.0 58 36906.0 59 33965.0 60 29486.0 61 24436.5 62 19758.5 63 15091.5 64 10679.0 65 8042.0 66 5854.0 67 4536.5 68 3304.5 69 1929.5 70 1422.5 71 1121.0 72 913.0 73 781.0 74 587.5 75 454.0 76 327.0 77 194.0 78 127.5 79 78.5 80 40.0 81 21.5 82 11.0 83 6.5 84 4.5 85 3.0 86 1.5 87 0.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04812470748942827 2 0.0 3 0.0 4 1.102513344545894E-4 5 0.011190510447140825 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005319626887433939 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.820106756367152E-4 24-25 5.236938386592997E-4 26-27 0.0017640213512734304 28-29 0.0027838461949783824 30-31 6.063823395002418E-4 32-33 0.0 34-35 0.0 36-37 8.268850084094206E-5 38-39 1.6537700168188412E-4 40-41 5.51256672272947E-5 42-43 0.010529002440413288 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.756283361364735E-5 52-53 5.51256672272947E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.410053378183576E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.006615080067275364 92-93 0.002315278023546377 94-95 0.0 96-97 0.0 98-99 0.0012127646790004835 100-101 0.009839931600072105 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1814037.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.94461471355435 #Duplication Level Percentage of deduplicated Percentage of total 1 71.44073132086616 33.537576067126174 2 14.647196814589552 13.75214022189014 3 5.434131665225253 7.6530965198017515 4 2.693376259438364 5.057580431118728 5 1.4828447577817645 3.480578791703938 6 0.9436778160480904 2.6580354892863616 7 0.6415490557239091 2.108209126856247 8 0.43778364434139866 1.6441267609202133 9 0.3341554323906816 1.4118118225219536 >10 1.74294431983424 14.864207551525357 >50 0.12568439519263686 4.028552196991199 >100 0.07133414484773691 5.9959864271524985 >500 0.0032956529273578143 1.080450382181727 >1k 9.416151221022327E-4 0.6684713472248406 >5k 1.1770189026277908E-4 0.48436269050859254 >10k+ 2.3540378052555816E-4 1.5748141731902119 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15955 0.8795300206114869 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12546 0.6916066210336393 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8766 0.4832315989144654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2810 0.1549031249086981 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2170 0.1196226978832295 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.51256672272947E-5 0.0 18-19 0.0 0.0 0.0 5.51256672272947E-5 0.0 20-21 0.0 0.0 0.0 5.51256672272947E-5 0.0 22-23 0.0 0.0 0.0 1.653770016818841E-4 0.0 24-25 0.0 0.0 0.0 3.5831683697741556E-4 0.0 26-27 0.0 0.0 0.0 7.441965075684785E-4 0.0 28-29 0.0 0.0 0.0 0.0017915841848870778 0.0 30-31 0.0 0.0 0.0 0.004547867546251813 0.0 32-33 0.0 0.0 0.0 0.007965658914344085 0.0 34-35 0.0 0.0 0.0 0.011466138783277298 0.0 36-37 0.0 0.0 0.0 0.013753853973210028 0.0 38-39 0.0 0.0 0.0 0.015655689492551694 0.0 40-41 0.0 0.0 0.0 0.018577349855598314 0.0 42-43 0.0 0.0 0.0 0.022353458060668005 0.0 44-45 0.0 0.0 0.0 0.026570571603556047 0.0 46-47 0.0 0.0 0.0 0.03249658083049023 0.0 48-49 0.0 0.0 0.0 0.039855857405334066 0.0 50-51 0.0 0.0 0.0 0.04975091467263347 0.0 52-53 0.0 0.0 0.0 0.0796841519770545 0.0 54-55 0.0 0.0 0.0 0.11342106032015885 0.0 56-57 0.0 0.0 0.0 0.1528634752212882 0.0 58-59 0.0 0.0 0.0 0.2128677639981985 0.0 60-61 0.0 0.0 0.0 0.3097511241501689 0.0 62-63 0.0 0.0 0.0 0.3696727244262383 0.0 64-65 0.0 0.0 0.0 0.4348037002552869 0.0 66-67 0.0 0.0 0.0 0.5694205796243406 0.0 68-69 0.0 0.0 0.0 0.7309663474339279 0.0 70-71 0.0 0.0 0.0 0.8613385504264797 0.0 72-73 0.0 0.0 0.0 1.0033698320376045 0.0 74-75 0.0 0.0 0.0 1.278226408832896 0.0 76-77 0.0 0.0 0.0 1.4695400369452223 0.0 78-79 0.0 0.0 0.0 1.6326568862707873 0.0 80-81 0.0 0.0 0.0 1.863633431953152 0.0 82-83 0.0 0.0 0.0 2.2451306119996453 0.0 84-85 0.0 0.0 0.0 2.622658744005773 0.0 86-87 0.0 0.0 0.0 3.1035199392294643 0.0 88-89 0.0 0.0 0.0 3.7289481967567366 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7620 0.0 45.270252 1 GTATCAA 13395 0.0 39.55154 1 TATCAAC 17515 0.0 30.239613 2 ATCAACG 17680 0.0 29.903666 3 TCAACGC 17865 0.0 29.620586 4 CAACGCA 18135 0.0 29.127201 5 AACGCAG 18615 0.0 28.399307 6 ACGCAGA 21155 0.0 24.835081 7 CGCAGAG 21170 0.0 24.750168 8 GCAGAGT 21925 0.0 23.44289 9 GTACATG 12105 0.0 23.394444 1 AGTACTT 12970 0.0 22.817682 12-13 GAGTACT 12665 0.0 22.748306 12-13 TACATGG 12185 0.0 22.259842 2 GTACTTT 13380 0.0 21.299564 14-15 ACATGGG 12135 0.0 21.25551 3 AGAGTAC 20685 0.0 20.88674 10-11 TACTTTT 14335 0.0 20.592972 14-15 ATGGGAG 3065 0.0 19.992668 5 CATTCCG 320 3.965397E-10 19.2982 9 >>END_MODULE