FastQCFastQC Report
Thu 26 May 2016
SRR937421_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937421_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046643
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157920.7716050136736109No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114550.5596970258125135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81780.3995811677952628No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28810.1407671000755872No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.12913830111064803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76350.045.4571231
GTATCAA137100.041.7866631
TATCAAC174300.032.6910632
ATCAACG176800.032.2272223
TCAACGC179600.031.8049554
CAACGCA182000.031.3878565
AACGCAG186250.030.7376376
GTACATG138350.028.3850061
TACATGG141450.027.4153922
ACGCAGA207900.027.3767577
CGCAGAG209250.027.0639048
ACATGGG144800.025.7296283
GCAGAGT216950.025.531489
GAGTACT115650.024.35765812-13
CATGGGA94550.023.9244564
AGTACTT118300.023.10931612-13
CATGGGG46900.022.899834
ATGGGCG3800.022.5121735
GTACTTT124700.022.43425214-15
GCGTATC1100.00286299321.6105291
Produced by FastQC (version 0.11.5)