FastQCFastQC Report
Thu 26 May 2016
SRR937421_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937421_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046643
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174520.8527134434290689No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139940.6837538349384822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100320.4901685345221419No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.15894320602078624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.11765608364526692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89300.044.9727481
GTATCAA152000.039.2413861
TATCAAC199600.029.7258192
ATCAACG200950.029.5497593
TCAACGC202600.029.3091014
CAACGCA206800.028.6908785
AACGCAG211950.028.0351436
ACGCAGA239700.024.6520167
CGCAGAG240000.024.4628648
GTACATG139900.024.4601421
TACATGG141500.023.6345962
GCAGAGT250050.023.270699
GAGTACT141500.022.72648812-13
AGTACTT144350.022.42586312-13
ACATGGG141600.022.4101683
GTACTTT146850.021.73572714-15
GTAAGGT7200.021.1129744
AGAGTAC233350.020.74275610-11
TACTTTT160450.020.0561914-15
CATGGGA93600.019.539654
Produced by FastQC (version 0.11.5)