FastQCFastQC Report
Thu 26 May 2016
SRR937420_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937420_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180647
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164930.7563351610783405No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118920.5453427354358591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85190.39066387177750456No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29700.1361981100104694No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27960.1282188267977348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78000.048.85621
GTATCAA144700.042.2291531
TATCAAC185850.032.6684342
ATCAACG186000.032.6676333
TCAACGC188300.032.2960664
CAACGCA192550.031.48385
AACGCAG197550.030.733636
ACGCAGA222200.027.0663537
CGCAGAG222150.026.8579838
GTACATG141850.025.9939521
GCAGAGT229900.025.291969
TACATGG143100.025.0981772
GAGTACT126700.023.84490612-13
ACATGGG145350.023.663753
AGTACTT129150.022.95119512-13
GTACTTT133750.022.35615314-15
ATGGGAG36100.022.1096635
AGAGTAC218550.021.01797110-11
CATGGGA95050.020.8936424
CAGAGTA228950.020.50931410-11
Produced by FastQC (version 0.11.5)