##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937420_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180647 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7752024972405 33.0 31.0 34.0 30.0 34.0 2 31.84869123705029 33.0 31.0 34.0 30.0 34.0 3 31.87972285289641 33.0 31.0 34.0 30.0 34.0 4 35.51681909084781 37.0 35.0 37.0 33.0 37.0 5 35.540195180604655 37.0 35.0 37.0 33.0 37.0 6 35.57860029615064 37.0 35.0 37.0 33.0 37.0 7 35.46700360030761 37.0 35.0 37.0 33.0 37.0 8 35.47657598868593 37.0 35.0 37.0 33.0 37.0 9 37.090725825867274 39.0 37.0 39.0 33.0 39.0 10-11 36.980618366934216 39.0 37.0 39.0 33.0 39.0 12-13 36.828957873511854 39.0 37.0 39.0 33.0 39.0 14-15 37.966086212027896 40.0 38.0 41.0 33.0 41.0 16-17 37.911315770044396 40.0 38.0 41.0 33.0 41.0 18-19 37.91880368532826 40.0 38.0 41.0 33.0 41.0 20-21 37.82867263706597 40.0 38.0 41.0 33.0 41.0 22-23 37.67884393943632 40.0 37.0 41.0 32.0 41.0 24-25 37.603854039649704 40.0 37.0 41.0 32.0 41.0 26-27 37.61038398236853 40.0 37.0 41.0 32.0 41.0 28-29 37.51833974045318 40.0 37.0 41.0 32.0 41.0 30-31 37.264470590609115 39.5 36.5 41.0 32.0 41.0 32-33 36.98189207148154 39.0 36.0 41.0 31.0 41.0 34-35 36.97900875290682 39.0 36.0 41.0 31.0 41.0 36-37 36.82835644650418 39.0 36.0 41.0 31.0 41.0 38-39 36.70001288608381 39.0 35.0 41.0 30.5 41.0 40-41 36.45091915381077 39.0 35.0 41.0 30.0 41.0 42-43 36.132532684107055 39.0 35.0 41.0 30.0 41.0 44-45 35.816955013810116 39.0 35.0 41.0 28.5 41.0 46-47 35.357641333053905 39.0 35.0 40.5 26.5 41.0 48-49 34.930660028881334 39.0 35.0 40.0 24.0 41.0 50-51 33.91106240487342 38.0 33.5 39.5 23.0 40.5 52-53 34.00722193917677 38.0 33.5 39.5 23.0 40.5 54-55 34.36774590293615 38.0 34.0 40.0 23.0 41.0 56-57 34.14841948284156 38.0 34.0 40.0 22.0 41.0 58-59 33.875680245358375 38.0 34.0 40.0 20.5 41.0 60-61 33.57018238165095 37.5 33.0 40.0 19.5 41.0 62-63 33.2496275188052 37.0 33.0 40.0 18.0 41.0 64-65 32.832730377727344 36.5 32.5 39.5 15.0 41.0 66-67 32.47941918155483 36.0 32.0 39.0 12.5 41.0 68-69 32.00607870049577 35.5 31.5 39.0 8.0 40.0 70-71 31.546411225659174 35.0 31.0 38.5 7.0 40.0 72-73 31.102554883940407 35.0 31.0 37.5 2.0 40.0 74-75 30.62462792006226 35.0 30.0 37.0 2.0 39.0 76-77 30.151511455086496 34.5 30.0 36.5 2.0 39.0 78-79 29.66696971128294 34.0 29.0 36.0 2.0 38.5 80-81 29.190247894317604 34.0 29.0 35.5 2.0 37.0 82-83 28.728485857637665 34.0 29.0 35.0 2.0 37.0 84-85 28.228624119355402 34.0 27.5 35.0 2.0 36.5 86-87 27.77413240198895 34.0 26.5 35.0 2.0 36.0 88-89 27.331207664514245 33.0 26.0 35.0 2.0 35.5 90-91 26.83813267346801 33.0 25.0 35.0 2.0 35.0 92-93 26.346718428062864 33.0 25.0 35.0 2.0 35.0 94-95 25.820601867243987 32.0 23.5 35.0 2.0 35.0 96-97 25.10510802527874 32.0 19.0 34.0 2.0 35.0 98-99 24.204138267220692 32.0 4.5 34.0 2.0 35.0 100-101 21.69621699431407 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08474759447246782 1101 2 -0.018634413099981373 1101 3 -0.03855581610856973 1101 4 -0.1044932845427482 1101 5 -0.12245355214255227 1101 6 -0.17668837218091227 1101 7 -0.11539907678861283 1101 8 -0.21358365398945267 1101 9 -0.2661912377102098 1101 10-11 -0.24206164143684106 1101 12-13 -0.17343639934939858 1101 14-15 -0.18905023251828368 1101 16-17 -0.25776208278924884 1101 18-19 -0.14063820048911424 1101 20-21 -0.055193966510373116 1101 22-23 -0.15525283077173668 1101 24-25 -0.07960503368160943 1101 26-27 -0.15356507634091088 1101 28-29 -0.18998292791584248 1101 30-31 -0.14426841896481335 1101 32-33 -0.3414861158532787 1101 34-35 -0.09253120398804526 1101 36-37 -0.02719094931588728 1101 38-39 -0.049546580593137435 1101 40-41 -0.07582927871394674 1101 42-43 0.058706304616272575 1101 44-45 0.1511716818505775 1101 46-47 0.1658072758677207 1101 48-49 0.06402750466809692 1101 50-51 0.14191528726694003 1101 52-53 0.14431146928431815 1101 54-55 0.07888284752667829 1101 56-57 0.08853106326382232 1101 58-59 0.08464624497765527 1101 60-61 0.18632652564284058 1101 62-63 0.1476095040897718 1101 64-65 0.13084307932699346 1101 66-67 -0.06909447601998409 1101 68-69 0.1292756049888517 1101 70-71 -0.006813849809834238 1101 72-73 -0.011747949163286364 1101 74-75 0.10040940001111665 1101 76-77 0.19522622072170037 1101 78-79 0.22270342251846031 1101 80-81 0.2813957178394162 1101 82-83 0.1218077907788171 1101 84-85 0.12110201490562389 1101 86-87 0.25285770692420684 1101 88-89 0.2345029576542217 1101 90-91 0.21194946667730008 1101 92-93 0.3700033453612228 1101 94-95 0.24917047836351003 1101 96-97 0.35326545077542804 1101 98-99 0.3938900573248283 1101 100-101 0.35494567133813604 1102 1 0.018775834514091372 1102 2 -0.028441895846075482 1102 3 -0.023299731836868176 1102 4 -0.14626589623907904 1102 5 -0.02256252417306115 1102 6 -0.2210036645891904 1102 7 0.011008478605504024 1102 8 -0.15655495802141672 1102 9 -0.21097874225070967 1102 10-11 -0.17667842312953752 1102 12-13 -0.175979080061353 1102 14-15 -0.10405204871879192 1102 16-17 -0.11628006317427975 1102 18-19 -0.15280674389630633 1102 20-21 -0.14473265158120086 1102 22-23 -0.23897967421525124 1102 24-25 -0.10684804130965375 1102 26-27 -0.2917779350405141 1102 28-29 -0.22630693808656588 1102 30-31 -0.12156591260810501 1102 32-33 -0.20109381654343395 1102 34-35 -0.060566075037812084 1102 36-37 -0.20917424027121 1102 38-39 -0.05935406333922799 1102 40-41 -0.18135052825989106 1102 42-43 -0.18829696454464084 1102 44-45 -0.028632168494503674 1102 46-47 -0.036880700884914575 1102 48-49 -0.055660108844435285 1102 50-51 -0.09509887909702996 1102 52-53 -0.045481483857713556 1102 54-55 0.09341245159496481 1102 56-57 0.048574652076020186 1102 58-59 -0.029229526907563752 1102 60-61 -0.005282628007719836 1102 62-63 0.2177148437192642 1102 64-65 0.2356378486695334 1102 66-67 0.04405481566181635 1102 68-69 -0.026372778592698154 1102 70-71 -0.10507029732163886 1102 72-73 0.0834209574840088 1102 74-75 0.02285763829662102 1102 76-77 0.07263268639551157 1102 78-79 0.005849081799247813 1102 80-81 -0.10363878997024756 1102 82-83 -0.06453438139698875 1102 84-85 -0.07940652123676273 1102 86-87 -0.006314105643902224 1102 88-89 -0.08133431078021047 1102 90-91 -0.05085474690787706 1102 92-93 -0.04336389038160249 1102 94-95 -0.06103656849446182 1102 96-97 0.02871041990001899 1102 98-99 0.035916937092355994 1102 100-101 0.36130237311801494 1103 1 0.07158782404327013 1103 2 -0.1045217261984881 1103 3 -0.05021557997243775 1103 4 -0.09030587355411512 1103 5 -0.17201255975971463 1103 6 -0.18631344740254008 1103 7 -0.08545403242450078 1103 8 -0.11117671941089924 1103 9 -0.07711237995930276 1103 10-11 -0.07440557383093704 1103 12-13 -0.018701556748908388 1103 14-15 -0.24515973201294372 1103 16-17 -0.05788001770125817 1103 18-19 -0.16913694217786457 1103 20-21 -0.2352648419991965 1103 22-23 -0.20314318180027158 1103 24-25 -0.22825070076553544 1103 26-27 -0.278083697299941 1103 28-29 -0.36318116323042915 1103 30-31 -0.2744475434401892 1103 32-33 -0.26561632619034015 1103 34-35 -0.04630557218007425 1103 36-37 -0.048819055308690906 1103 38-39 -0.1330363944787578 1103 40-41 -0.014154989541317775 1103 42-43 0.12272046425092498 1103 44-45 -0.026182696023077767 1103 46-47 0.020834170955190245 1103 48-49 0.0013142514442137099 1103 50-51 -0.08034871082823258 1103 52-53 -0.1271853075861813 1103 54-55 0.15543623587530675 1103 56-57 0.0023571813582279333 1103 58-59 -0.16790112478172858 1103 60-61 -0.02243616574725138 1103 62-63 0.07401923577459968 1103 64-65 0.036023810964437075 1103 66-67 -0.0386009204892801 1103 68-69 0.035976876586921946 1103 70-71 -0.05921578035151498 1103 72-73 0.07479575898696567 1103 74-75 0.017210879774260945 1103 76-77 0.08840420794200554 1103 78-79 -0.0794650936850978 1103 80-81 -0.24632249432058018 1103 82-83 -0.2066318474357587 1103 84-85 -0.07953523737844037 1103 86-87 0.11427251638207991 1103 88-89 0.05365062198600512 1103 90-91 0.12854477153053168 1103 92-93 0.02425124291405467 1103 94-95 -0.15421968145211196 1103 96-97 -0.22510313584259123 1103 98-99 0.11859300438040066 1103 100-101 0.5248189782877617 1104 1 -0.019954354863482848 1104 2 -0.13853644712996527 1104 3 -0.10272627655264088 1104 4 -0.1441580723681284 1104 5 -0.08353558367453928 1104 6 -0.10571389180332602 1104 7 -0.10777074818580701 1104 8 -0.05069410189772583 1104 9 -0.0751306682826538 1104 10-11 -0.0545232548645771 1104 12-13 -0.05051385772289052 1104 14-15 0.0027637450066677616 1104 16-17 -0.08691784461374397 1104 18-19 -0.02885506972611296 1104 20-21 -0.07059120737522306 1104 22-23 -0.22207855344192495 1104 24-25 -0.02679284485633815 1104 26-27 -0.06915121351733688 1104 28-29 -0.05968085423883451 1104 30-31 -0.07196401573850864 1104 32-33 -0.015530762874419679 1104 34-35 0.19262194411193434 1104 36-37 0.1368096648692685 1104 38-39 0.17415697022209287 1104 40-41 0.23245364120937495 1104 42-43 0.25943735396766243 1104 44-45 0.3698089929112953 1104 46-47 0.46437567157254733 1104 48-49 0.47898276998805756 1104 50-51 0.2768120760869337 1104 52-53 0.26903517190411463 1104 54-55 0.1684310576238417 1104 56-57 0.2135534793032221 1104 58-59 0.20459919850927832 1104 60-61 0.2928227654775668 1104 62-63 0.367501253955993 1104 64-65 0.43046782193643196 1104 66-67 0.36277838192334144 1104 68-69 0.38278131599410514 1104 70-71 0.3139764323142842 1104 72-73 0.403509968496941 1104 74-75 0.4545492342727897 1104 76-77 0.44257346508145545 1104 78-79 0.37105871545384517 1104 80-81 0.5231484057588744 1104 82-83 0.5863360914224529 1104 84-85 0.6178568455979274 1104 86-87 0.6700023675811764 1104 88-89 0.542149777699116 1104 90-91 0.5687737827947359 1104 92-93 0.6267048817408067 1104 94-95 0.788055932831881 1104 96-97 0.7681253942153923 1104 98-99 1.0416055263113435 1104 100-101 1.476177126899664 1105 1 0.015884120291485004 1105 2 0.0020143435915080943 1105 3 -0.043237261717440134 1105 4 -0.04397859021699446 1105 5 -0.08523527445095169 1105 6 -0.05693877306848094 1105 7 -0.01928715158167904 1105 8 -0.11798481103404157 1105 9 0.06523716623012632 1105 10-11 -0.0632781234153299 1105 12-13 0.030492560517274114 1105 14-15 -0.06081832651866392 1105 16-17 -0.07851086883604097 1105 18-19 -0.06764600678658184 1105 20-21 -0.034922133110910636 1105 22-23 0.03721455052135525 1105 24-25 -0.07329424162228548 1105 26-27 -0.027733710371201425 1105 28-29 -0.0805164332635826 1105 30-31 -0.018659797388465904 1105 32-33 -0.11084697029448876 1105 34-35 -0.15524769561206142 1105 36-37 -0.12334399886533731 1105 38-39 -0.15852990160660596 1105 40-41 0.0264562327913751 1105 42-43 0.11124063334811751 1105 44-45 0.06289581318109327 1105 46-47 -0.00975680430710213 1105 48-49 0.012090376161104643 1105 50-51 -0.002516843137001956 1105 52-53 -0.028570780357782155 1105 54-55 0.0400083804046929 1105 56-57 0.08166210171926735 1105 58-59 0.11528888984413044 1105 60-61 0.19190642597837382 1105 62-63 0.14964829944861435 1105 64-65 0.15972956522501391 1105 66-67 0.099305369831022 1105 68-69 0.07347144239784953 1105 70-71 0.05003442245185852 1105 72-73 0.30220515144016247 1105 74-75 0.2954024141068956 1105 76-77 0.18914916447092978 1105 78-79 0.20010058661371133 1105 80-81 0.2628771273186814 1105 82-83 0.3517884234566395 1105 84-85 0.31355773183974733 1105 86-87 0.2899569483371067 1105 88-89 0.3023359904309224 1105 90-91 0.32654536318572625 1105 92-93 0.5192377521694986 1105 94-95 0.5785853597227728 1105 96-97 0.6859201826095713 1105 98-99 0.7571494500872724 1105 100-101 1.0936047366311819 1106 1 -0.17158470114906166 1106 2 -0.15902496453531967 1106 3 -0.19629990122179564 1106 4 -0.08937115101721105 1106 5 -0.050061357069587586 1106 6 -0.18389930790075937 1106 7 -0.43470730619491604 1106 8 -0.4027937704328508 1106 9 -0.5887349417232386 1106 10-11 -0.8914170412091096 1106 12-13 -0.4713152106294842 1106 14-15 -0.5160615832153255 1106 16-17 -0.31624493089672967 1106 18-19 -0.6514370458749994 1106 20-21 -0.5658874627328956 1106 22-23 -0.47262949995216275 1106 24-25 -0.4140871972578708 1106 26-27 -0.7616587204653982 1106 28-29 -0.4662552296028011 1106 30-31 -0.6346597966681031 1106 32-33 -0.6354920344741473 1106 34-35 -0.430397649850498 1106 36-37 -0.7431046952048064 1106 38-39 -0.7320584446885832 1106 40-41 -0.6922952417181136 1106 42-43 -0.5228516649128707 1106 44-45 -0.5106683882677956 1106 46-47 -0.4882941593279284 1106 48-49 -0.2140365272843212 1106 50-51 -0.5015216505332063 1106 52-53 -0.4355408925231643 1106 54-55 -0.6817589275002618 1106 56-57 -0.5409294727489069 1106 58-59 -0.5437253652183571 1106 60-61 -0.6544750782068007 1106 62-63 -0.6646640865751792 1106 64-65 -0.4857115485838861 1106 66-67 -0.30834036033166967 1106 68-69 -0.35618819668905033 1106 70-71 -0.04662685583936721 1106 72-73 -0.37296099313758546 1106 74-75 -0.11638193777707428 1106 76-77 -0.12179602717838378 1106 78-79 -0.27560535000011654 1106 80-81 -0.654708822852534 1106 82-83 -0.4554770228294345 1106 84-85 -0.6277999226609339 1106 86-87 -0.5431830554160264 1106 88-89 -0.21284168728731956 1106 90-91 -0.10959437366993185 1106 92-93 0.031656949049427396 1106 94-95 -0.011677943099243748 1106 96-97 0.2209666843616951 1106 98-99 0.17117334551533148 1106 100-101 0.4837911938715749 1107 1 0.08013326071121085 1107 2 -0.00920080264729961 1107 3 0.03259465209665535 1107 4 0.02146533845829879 1107 5 0.0785980192239748 1107 6 0.04667794792391078 1107 7 0.08509755764664106 1107 8 0.04662654285367296 1107 9 0.06764688669500174 1107 10-11 0.13312920243703985 1107 12-13 0.08215172985616448 1107 14-15 0.1456295012706832 1107 16-17 0.10061609220932866 1107 18-19 0.14243906364801262 1107 20-21 0.13754410373414316 1107 22-23 0.13762334688235 1107 24-25 0.1476717113189281 1107 26-27 0.18892773145746844 1107 28-29 0.12342536078211452 1107 30-31 0.23475302902226503 1107 32-33 0.2838870225015313 1107 34-35 0.12285343458963638 1107 36-37 0.07333023437395525 1107 38-39 0.21624646903206468 1107 40-41 0.2115212536970219 1107 42-43 0.1349878368007964 1107 44-45 -0.07543523507249716 1107 46-47 -6.189707630710473E-5 1107 48-49 0.1874680438186047 1107 50-51 0.109218520706861 1107 52-53 0.1269229164731911 1107 54-55 0.12717378618575736 1107 56-57 0.08198377548038849 1107 58-59 0.03038779036195649 1107 60-61 0.2255625661702183 1107 62-63 0.3359496555143977 1107 64-65 0.4291574643491245 1107 66-67 0.32561135900616023 1107 68-69 0.26512605416721513 1107 70-71 0.4554333797504526 1107 72-73 0.4155866540028512 1107 74-75 0.3500359641136299 1107 76-77 0.3544428947912337 1107 78-79 0.38145156605051156 1107 80-81 0.44515598762414044 1107 82-83 0.6975795331744585 1107 84-85 0.8011584988207865 1107 86-87 0.8098006312561665 1107 88-89 0.8045449008570564 1107 90-91 0.8296594925325707 1107 92-93 0.9091189406124087 1107 94-95 1.0131099561197772 1107 96-97 1.1120453797999907 1107 98-99 1.4313581389299195 1107 100-101 1.7374785196531022 1108 1 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0.8265336101070488 2309 34-35 0.8709714644695197 2309 36-37 0.9045047837626328 2309 38-39 0.8733770164070975 2309 40-41 0.8193417198920514 2309 42-43 0.706165534329422 2309 44-45 0.8721640379026354 2309 46-47 0.8978275391675155 2309 48-49 0.9595104817431661 2309 50-51 0.9697481235151528 2309 52-53 0.9748609991727903 2309 54-55 0.8845283006371503 2309 56-57 0.9337716756855983 2309 58-59 0.8680900515956083 2309 60-61 0.9019042528870429 2309 62-63 0.8600228412428024 2309 64-65 0.9999291682684301 2309 66-67 1.0080383752868727 2309 68-69 1.0306372453472612 2309 70-71 1.0895880158023914 2309 72-73 1.143328171335611 2309 74-75 1.087305699197863 2309 76-77 1.1441067029717757 2309 78-79 1.181778918541756 2309 80-81 1.2441088879988165 2309 82-83 1.2705114765852912 2309 84-85 1.3262889314155544 2309 86-87 1.2671871053581398 2309 88-89 1.418030667376108 2309 90-91 1.5304756800436046 2309 92-93 1.643755591407377 2309 94-95 1.7774212568401282 2309 96-97 1.8983203629624334 2309 98-99 1.8638350076909482 2309 100-101 2.1704160689987404 2310 1 0.39994144580186486 2310 2 0.3473563649914695 2310 3 0.35667702929346845 2310 4 0.3092796755242091 2310 5 0.35485951904896496 2310 6 0.31184903057447144 2310 7 0.36646228444836026 2310 8 0.26757583248959094 2310 9 0.370951018584357 2310 10-11 0.35929668536454784 2310 12-13 0.34247889844021984 2310 14-15 0.33035673730163495 2310 16-17 0.48555780428768713 2310 18-19 0.24781814591024443 2310 20-21 0.38046792705390686 2310 22-23 0.4239281123638392 2310 24-25 0.35220955950718036 2310 26-27 0.4405311921990247 2310 28-29 0.5185147662529843 2310 30-31 0.5189928717483454 2310 32-33 0.47743409098823975 2310 34-35 0.3770803627663497 2310 36-37 0.5118050529187883 2310 38-39 0.48638409648500414 2310 40-41 0.5807531381993059 2310 42-43 0.6645360958275646 2310 44-45 0.662383445165311 2310 46-47 0.4914989367105065 2310 48-49 0.43804479149819997 2310 50-51 0.39332647152171774 2310 52-53 0.26917967968601175 2310 54-55 0.3236189638598077 2310 56-57 0.32058262990492636 2310 58-59 0.45198076362078865 2310 60-61 0.3727271136244781 2310 62-63 0.5443618685137679 2310 64-65 0.40315623548696067 2310 66-67 0.2818117478477902 2310 68-69 0.276029651079984 2310 70-71 0.3664635034797534 2310 72-73 0.5367007925602536 2310 74-75 0.5202237935240568 2310 76-77 0.6393396422664424 2310 78-79 0.6384999592046512 2310 80-81 0.4834183739671616 2310 82-83 0.4789656564847746 2310 84-85 0.5860916522957069 2310 86-87 0.6052509670855457 2310 88-89 0.7461075230087637 2310 90-91 0.9641074881587421 2310 92-93 0.8499713264189239 2310 94-95 1.0464696678353533 2310 96-97 0.9288261185990798 2310 98-99 0.9197535668473158 2310 100-101 1.0464897710231185 2311 1 0.5550321640504805 2311 2 0.5696310688451192 2311 3 0.5322955628067412 2311 4 0.5147677546964999 2311 5 0.42388036175402277 2311 6 0.5559485957266688 2311 7 0.608027736540123 2311 8 0.527127489409672 2311 9 0.6870103978016289 2311 10-11 0.6591617325980152 2311 12-13 0.7119945679558839 2311 14-15 0.8469709223368724 2311 16-17 0.915382365691201 2311 18-19 0.8102225543672859 2311 20-21 0.7823122815298404 2311 22-23 0.9054208399091976 2311 24-25 0.8310369699135407 2311 26-27 0.8721288830072425 2311 28-29 1.0378130118670157 2311 30-31 0.8615142392697166 2311 32-33 0.934916472154832 2311 34-35 0.8326584104496604 2311 36-37 0.8548662315062785 2311 38-39 0.9455308951054846 2311 40-41 0.9119984523393612 2311 42-43 0.9300556639787132 2311 44-45 0.6418856194043272 2311 46-47 0.5307851865230688 2311 48-49 0.40660079689630635 2311 50-51 0.42741703982281365 2311 52-53 0.38502913237756786 2311 54-55 0.5437306747609867 2311 56-57 0.4059814903037875 2311 58-59 0.5058943938502196 2311 60-61 0.49074330795646404 2311 62-63 0.3933647175166186 2311 64-65 0.4469521569144561 2311 66-67 0.5540864517014441 2311 68-69 0.4070125570628882 2311 70-71 0.4680679419262965 2311 72-73 0.45556044168306187 2311 74-75 0.43056267491557065 2311 76-77 0.28055271769004264 2311 78-79 0.5844626222725786 2311 80-81 0.48573131443799866 2311 82-83 0.6262478577143433 2311 84-85 0.735424385838968 2311 86-87 0.7863613284590656 2311 88-89 0.7813301950735401 2311 90-91 0.700934598670063 2311 92-93 0.917635144082741 2311 94-95 1.0895233490469316 2311 96-97 1.0174301072030687 2311 98-99 1.1443074729801666 2311 100-101 1.2925199104743115 2312 1 0.47621653523485463 2312 2 0.5524149516290038 2312 3 0.4639805444917222 2312 4 0.4852805752093161 2312 5 0.5144786523523095 2312 6 0.4855578753359495 2312 7 0.3641327426451326 2312 8 0.44171845400063603 2312 9 0.47510563589686683 2312 10-11 0.5626415860778664 2312 12-13 0.5373303432916572 2312 14-15 0.43756921293515916 2312 16-17 0.5197779700867997 2312 18-19 0.5572616264063583 2312 20-21 0.6377457369632964 2312 22-23 0.6489190084073684 2312 24-25 0.7569527208292897 2312 26-27 0.8801264410047978 2312 28-29 0.779174428228437 2312 30-31 0.4965569743124547 2312 32-33 0.5004537126920781 2312 34-35 0.11409919189043904 2312 36-37 0.20498833162837826 2312 38-39 0.30490207947666903 2312 40-41 0.4123647527056633 2312 42-43 0.28643539535844553 2312 44-45 0.6624289649476722 2312 46-47 0.4802662610041466 2312 48-49 0.37433583963134964 2312 50-51 0.5399323023380731 2312 52-53 0.34110361345204865 2312 54-55 0.2610078420381612 2312 56-57 0.18968112400342108 2312 58-59 0.32970391765973517 2312 60-61 0.37789227010542703 2312 62-63 0.2935478676997718 2312 64-65 0.4883746233369237 2312 66-67 0.3981951208101151 2312 68-69 0.28808703813736614 2312 70-71 0.12606549992385752 2312 72-73 0.2756153867380071 2312 74-75 0.17210724146013945 2312 76-77 0.25491169204902064 2312 78-79 0.457631120441075 2312 80-81 0.5050536587603389 2312 82-83 0.5931587246252121 2312 84-85 0.5846002100147913 2312 86-87 0.8456409377386755 2312 88-89 0.563656202399546 2312 90-91 0.8776440003794654 2312 92-93 1.2879037643513591 2312 94-95 1.3350972196208026 2312 96-97 1.3763739411469018 2312 98-99 1.2242220028946988 2312 100-101 1.404363622318023 2313 1 0.41445494002255145 2313 2 0.46843027433446593 2313 3 0.4628616516474757 2313 4 0.46214496668638105 2313 5 0.42836904599622727 2313 6 0.5267698910197964 2313 7 0.5828005185312151 2313 8 0.501384140426886 2313 9 0.6842386390439543 2313 10-11 0.6070264852162879 2313 12-13 0.5639838541001012 2313 14-15 0.7295293068318713 2313 16-17 0.7404997365832173 2313 18-19 0.8357176187708006 2313 20-21 0.7360245121786875 2313 22-23 0.7760262028561087 2313 24-25 0.6822851642075065 2313 26-27 0.8000353173397343 2313 28-29 0.7985035439483923 2313 30-31 0.743171275504217 2313 32-33 0.722940879281353 2313 34-35 0.6655553648254866 2313 36-37 0.7733310804288749 2313 38-39 1.0068090340244638 2313 40-41 0.9138222154307343 2313 42-43 0.8884416637379573 2313 44-45 0.7903906452861094 2313 46-47 0.6872646616645213 2313 48-49 0.7536695341058888 2313 50-51 0.8940506018789165 2313 52-53 0.9256364515060156 2313 54-55 0.707610535119727 2313 56-57 0.8812568604735205 2313 58-59 0.9173177416291978 2313 60-61 1.030210022775293 2313 62-63 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0.6662976721871985 2314 22-23 0.4944811441799928 2314 24-25 0.48860606219271574 2314 26-27 0.5768419574184875 2314 28-29 0.627231421539598 2314 30-31 0.7412261256535615 2314 32-33 0.7306596002649144 2314 34-35 0.7201691875553493 2314 36-37 0.6951580110355451 2314 38-39 0.6339632420366357 2314 40-41 0.49024940445846 2314 42-43 0.6342639964634529 2314 44-45 0.5053755008052434 2314 46-47 0.5006211708546857 2314 48-49 0.6724738612602366 2314 50-51 0.5939703347319494 2314 52-53 0.7077978472034729 2314 54-55 0.7414737621387104 2314 56-57 0.7703406683246001 2314 58-59 0.6661161815938428 2314 60-61 0.49203418458490944 2314 62-63 0.4811429705053598 2314 64-65 0.5302216762046257 2314 66-67 0.6917645709522446 2314 68-69 0.8373768402071526 2314 70-71 0.6409008102317628 2314 72-73 0.7335588531207122 2314 74-75 0.7325449834904134 2314 76-77 0.7753746154110672 2314 78-79 0.768247066581246 2314 80-81 0.6939816555715765 2314 82-83 0.5053884726373283 2314 84-85 0.7777483710938498 2314 86-87 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0.4404340214853306 2315 46-47 0.2616357067757704 2315 48-49 0.40478455758006504 2315 50-51 0.6014717592579615 2315 52-53 0.4444540673459869 2315 54-55 0.08417335406236504 2315 56-57 0.3202303754765836 2315 58-59 0.437583357060916 2315 60-61 0.47276207421435856 2315 62-63 0.551809533570129 2315 64-65 0.37501560916269483 2315 66-67 0.2405714944364874 2315 68-69 0.3546830327224946 2315 70-71 0.3477966673071968 2315 72-73 0.41117638371204634 2315 74-75 0.48556778454024396 2315 76-77 0.5030304211460077 2315 78-79 0.5977708719938697 2315 80-81 0.4139712809931808 2315 82-83 0.5019469472721312 2315 84-85 0.5089611751366263 2315 86-87 0.6900727740183399 2315 88-89 0.9620924581727586 2315 90-91 1.00849684318883 2315 92-93 0.9065333231983104 2315 94-95 1.122956552317092 2315 96-97 1.1642830429147004 2315 98-99 1.1803953585245743 2315 100-101 1.3452280115519741 2316 1 -0.1355910207474622 2316 2 -0.1560265529290028 2316 3 0.04463478776732188 2316 4 0.05168585277964155 2316 5 0.010246638411558706 2316 6 0.05506900067231868 2316 7 0.2422074726616117 2316 8 0.0798782984711579 2316 9 -0.0013308186949245737 2316 10-11 -0.20794058256255 2316 12-13 0.14003373482999848 2316 14-15 0.14226176956946546 2316 16-17 -0.027443955775900974 2316 18-19 0.13212945006058874 2316 20-21 0.17289397599503076 2316 22-23 0.14382014287161127 2316 24-25 0.34550049991104714 2316 26-27 0.3432474623782511 2316 28-29 0.18926932254662887 2316 30-31 0.23649556861997922 2316 32-33 0.09398625889452461 2316 34-35 0.1699748157272296 2316 36-37 -0.10746054635448132 2316 38-39 -0.08857000938376558 2316 40-41 -0.22216468764901975 2316 42-43 -0.2978922241633555 2316 44-45 -0.01730863833362406 2316 46-47 -0.10785476036929964 2316 48-49 -0.20909084695067293 2316 50-51 -0.22306009900384538 2316 52-53 -0.13811417523175606 2316 54-55 -0.22068306683721772 2316 56-57 0.096247858240055 2316 58-59 0.2575789013599632 2316 60-61 0.14164779721046727 2316 62-63 0.1439663273415306 2316 64-65 0.2475869590055737 2316 66-67 -0.1004816339346064 2316 68-69 0.07713536844783775 2316 70-71 0.20116892939621778 2316 72-73 -0.006683355512191014 2316 74-75 0.049042469123520505 2316 76-77 -0.015252609668678474 2316 78-79 -0.0048245738107333125 2316 80-81 0.25357630655483376 2316 82-83 0.0844961358655354 2316 84-85 0.16639691923868583 2316 86-87 0.1501444169994386 2316 88-89 -0.11947471738185911 2316 90-91 -0.10937989805220028 2316 92-93 0.1807875795652727 2316 94-95 0.4063435299447846 2316 96-97 0.5347232778942974 2316 98-99 0.9284166755359742 2316 100-101 0.871924109295982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7607.0 3 1847.0 4 1331.0 5 1556.0 6 1814.0 7 2203.0 8 2870.0 9 3584.0 10 4588.0 11 6225.0 12 8739.0 13 12017.0 14 13622.0 15 17579.0 16 23226.0 17 31448.0 18 26469.0 19 16327.0 20 15775.0 21 15961.0 22 16971.0 23 18700.0 24 20791.0 25 23314.0 26 27190.0 27 31843.0 28 37719.0 29 45388.0 30 54803.0 31 68231.0 32 85208.0 33 112190.0 34 152249.0 35 211749.0 36 303274.0 37 407116.0 38 312380.0 39 36740.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4494904063649 18.780799583738787 15.08418303910217 21.685526970794147 2 14.987493802045016 20.474504864389434 41.12133470519333 23.416666628372226 3 18.472732452482536 26.164806863008632 30.484146293832897 24.87831439067594 4 12.100986224472447 16.729605758352488 37.969596237072516 33.19981178010254 5 12.946595557548848 38.15602159006629 33.80115486451985 15.096227987865007 6 30.382211452278497 36.81119064976039 17.94951466306797 14.857083234893148 7 27.65602146751696 34.29167398277046 20.62281723468743 17.429487315025145 8 26.798479646660322 33.917621483493505 19.97361902004311 19.31027984980306 9 27.50925814436363 16.08389455136719 20.371272499657618 36.035574804611564 10-11 24.647501497230984 27.160643347399414 27.03996859836658 21.151886557003017 12-13 26.17353882471688 24.72604902505581 27.961936200597243 21.138475949630067 14-15 22.93199953951116 25.36215815769537 27.337756385318862 24.36808591747461 16-17 21.269475389680153 29.084083583735985 27.5369456429938 22.109495383590055 18-19 21.177191047382692 26.663656418033916 31.393285537019167 20.765866997564224 20-21 22.317139777499545 25.63844744617575 31.388156608298424 20.656256168026285 22-23 21.87266852185406 25.768883723591323 31.326518725007872 21.031929029546742 24-25 21.449350218470244 25.914658173397154 31.529080311163572 21.106911296969034 26-27 21.247202422805252 26.210092877265062 31.378521960119304 21.164182739810386 28-29 21.004007758137313 26.307645993620675 31.61650074915662 21.071845499085384 30-31 21.16113097136722 26.083024858463123 31.58529119506857 21.170552975101092 32-33 21.050544010052455 26.17370174562124 31.32974413396164 21.446010110364664 34-35 21.4330037074864 26.271650369231814 31.03512229740972 21.26022362587206 36-37 21.571474821243154 26.001409421248074 31.041802184258277 21.3853135732505 38-39 21.291089580288354 26.194829309113953 31.09928133660317 21.414799773994527 40-41 21.34997201459101 26.243903178756455 31.06208073583704 21.344044070815492 42-43 21.29033770126199 26.339737979426488 30.68963702818478 21.680287291126746 44-45 21.332405820442148 26.599810360842962 30.365521891464446 21.70226192725045 46-47 21.534164242823156 26.928610301120422 29.744811565318752 21.79241389073767 48-49 21.776802085358685 27.006263706310452 29.296168461386067 21.920765746944795 50-51 21.792054033692455 27.215625239165693 29.087535620605813 21.904785106536035 52-53 21.977940642380407 27.378148069484403 28.861375988289932 21.782535299845257 54-55 21.540422838549272 27.284786871968734 28.98706240259685 22.18772788688514 56-57 21.732775754512137 27.619659248876992 28.701928931677443 21.945636064933424 58-59 21.901157281107313 27.130758406800105 28.897269046319963 22.070815265772623 60-61 21.815656087641305 27.12994852304701 29.291760011908984 21.762635377402702 62-63 21.808725226938975 27.15235926475264 29.27698869118839 21.761926817119992 64-65 21.624843411186088 27.163808726979177 29.259945127006652 21.951402734828086 66-67 21.535446773269072 27.47240654868497 28.905462636129414 22.086684041916545 68-69 21.559682136367083 27.40457313781213 29.108231234535854 21.927513491284937 70-71 21.49278063268712 27.480393940730615 28.91583592480683 22.110989501775435 72-73 21.66788698735331 27.479638411360725 28.667182364156712 22.185292237129254 74-75 21.448134542761892 27.727239413690135 28.61236981568858 22.212256227859392 76-77 21.631032526488074 27.52443501951837 28.6413791376162 22.20315331637735 78-79 21.628767957054027 27.527865414259583 28.597401946897 22.24596468178939 80-81 21.45890857559562 27.941667735014754 28.422309497453657 22.17711419193597 82-83 21.547237017677354 27.74083508857056 28.427100678936785 22.284827214815305 84-85 21.55018050840098 27.487866168769497 28.35553331651621 22.606420006313314 86-87 21.583027710750915 27.452176058826584 28.310362432156843 22.65443379826566 88-89 21.647442324676483 27.22567977524476 28.410616689197944 22.716261210880813 90-91 21.395734860145957 27.35183899718594 28.668705694964213 22.583720447703886 92-93 21.5159325286235 26.976833307984162 28.773093975888113 22.73414018750422 94-95 21.309834406868912 26.976154000968616 28.763501138171332 22.950510453991136 96-97 21.36505068431523 26.916843166061383 28.667185633860683 23.05092051576271 98-99 21.1658175621709 26.896192954286224 28.706632941455446 23.23135654208743 100-101 21.31247491653394 26.59337872306406 29.166474269159405 22.92767209124259 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3382.0 1 2085.0 2 876.5 3 924.0 4 777.0 5 780.0 6 1262.0 7 2651.0 8 4457.0 9 5213.5 10 4681.5 11 4106.5 12 4201.5 13 4533.0 14 4861.5 15 5099.5 16 5266.5 17 5506.0 18 5764.0 19 6141.5 20 6597.0 21 7197.0 22 8267.5 23 9665.5 24 11438.0 25 13710.0 26 16720.0 27 20004.5 28 22537.5 29 25522.0 30 29479.5 31 33138.5 32 36652.0 33 40316.5 34 45301.5 35 48756.5 36 51908.0 37 56754.0 38 59805.0 39 60684.5 40 61500.0 41 64850.5 42 68619.5 43 71999.5 44 74667.5 45 76498.0 46 75637.5 47 76028.5 48 82118.0 49 82204.0 50 76641.0 51 72859.0 52 70189.5 53 68961.5 54 67015.0 55 62261.0 56 55776.0 57 49499.0 58 45129.0 59 40968.0 60 35596.5 61 30030.5 62 24519.0 63 18746.0 64 13713.5 65 10391.5 66 7637.5 67 5881.5 68 4296.0 69 2778.5 70 2077.5 71 1566.0 72 1160.0 73 952.0 74 752.0 75 574.5 76 406.0 77 256.0 78 173.0 79 120.0 80 76.0 81 53.0 82 31.0 83 19.5 84 21.0 85 16.0 86 12.0 87 9.5 88 5.0 89 3.0 90 4.0 91 3.5 92 2.5 93 2.5 94 2.0 95 1.5 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23341696294723538 2 0.20755307943009574 3 0.19911521672237642 4 0.2049391763086827 5 0.20457231271269488 6 0.23135335521980405 7 0.23300424140174913 8 0.22181490172412133 9 0.21667881138029219 10-11 0.2276388613104276 12-13 0.22855602030039707 14-15 0.2154635757185826 16-17 0.22857894927514633 18-19 0.2176876862692586 20-21 0.21124464436472296 22-23 0.21032748537475346 24-25 0.20601683812189686 26-27 0.2135604708143959 28-29 0.22319064020907559 30-31 0.224199515098042 32-33 0.22286963456258627 34-35 0.23153678701779792 36-37 0.20986890587976872 38-39 0.21064849102124275 40-41 0.2069569260866156 42-43 0.2107860648697382 44-45 0.22635483872447032 46-47 0.2310782075228132 48-49 0.2337838265432232 50-51 0.23458634065944647 52-53 0.24877937602922434 54-55 0.23334817602298766 56-57 0.2096625451070256 58-59 0.19303903841382855 60-61 0.1898060529741861 62-63 0.19677646129795423 64-65 0.19106714658539414 66-67 0.19166329992887432 68-69 0.194529421772529 70-71 0.19622616590397254 72-73 0.1974872595151806 74-75 0.1998260149396028 76-77 0.2118866556577016 78-79 0.20716328685935873 80-81 0.20131639829830322 82-83 0.19732675669193595 84-85 0.19691403514644967 86-87 0.20581047734915373 88-89 0.20237113113676813 90-91 0.21142807616271686 92-93 0.20067438700532456 94-95 0.19943622236886574 96-97 0.20773651122808962 98-99 0.2122764482284386 100-101 0.19092957273689873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180647.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.93480748579362 #Duplication Level Percentage of deduplicated Percentage of total 1 71.54340355464704 33.57875872715802 2 14.3495706467084 13.469886716141074 3 5.517380818251735 7.768716195913669 4 2.7059424375545196 5.0801154949704195 5 1.5273816643846199 3.584368218761159 6 0.9441791356142886 2.6588919577295793 7 0.6679043658486092 2.194357398101788 8 0.46144942860853433 1.7326432076936822 9 0.33105660663682346 1.3984270289459473 >10 1.7402168915961365 14.744015362767025 >50 0.13636389488071438 4.368312051583365 >100 0.07085822178251723 6.036707512262882 >500 0.003121697080775206 1.0184253070160743 >1k 8.779773039680222E-4 0.6766761503569888 >5k 9.755303377422467E-5 0.39005373333048166 >10k+ 1.9510606754844935E-4 1.2996449372679564 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16493 0.7563351610783405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11892 0.5453427354358591 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8519 0.39066387177750456 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2970 0.1361981100104694 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2796 0.1282188267977348 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.171589899694907E-5 0.0 22-23 0.0 0.0 0.0 3.2100564648932173E-4 0.0 24-25 0.0 0.0 0.0 4.815084697339826E-4 0.0 26-27 0.0 0.0 0.0 8.254430909725417E-4 0.0 28-29 0.0 0.0 0.0 0.002040678752682117 0.0 30-31 0.0 0.0 0.0 0.00545709599031847 0.0 32-33 0.0 0.0 0.0 0.00949259554618423 0.0 34-35 0.0 0.0 0.0 0.014101319470780919 0.0 36-37 0.0 0.0 0.0 0.01685279644068939 0.0 38-39 0.0 0.0 0.0 0.019214480839860833 0.0 40-41 0.0 0.0 0.0 0.023364625269472777 0.0 42-43 0.0 0.0 0.0 0.02795042021932023 0.0 44-45 0.0 0.0 0.0 0.03271964696716158 0.0 46-47 0.0 0.0 0.0 0.04021742171016217 0.0 48-49 0.0 0.0 0.0 0.05078767906956055 0.0 50-51 0.0 0.0 0.0 0.06502657238883688 0.0 52-53 0.0 0.0 0.0 0.09790672217924312 0.0 54-55 0.0 0.0 0.0 0.13979795904609962 0.0 56-57 0.0 0.0 0.0 0.1906085670904094 0.0 58-59 0.0 0.0 0.0 0.2603585082775892 0.0 60-61 0.0 0.0 0.0 0.3598014717650312 0.0 62-63 0.0 0.0 0.0 0.43106472528566064 0.0 64-65 0.0 0.0 0.0 0.5046667342307123 0.0 66-67 0.0 0.0 0.0 0.6383426570187656 0.0 68-69 0.0 0.0 0.0 0.8074667747691395 0.0 70-71 0.0 0.0 0.0 0.9507040800276249 0.0 72-73 0.0 0.0 0.0 1.1037779154535328 0.0 74-75 0.0 0.0 0.0 1.358908617488296 0.0 76-77 0.0 0.0 0.0 1.558482413705657 0.0 78-79 0.0 0.0 0.0 1.73966717217413 0.0 80-81 0.0 0.0 0.0 1.9668933119390712 0.0 82-83 0.0 0.0 0.0 2.3266030678051055 0.0 84-85 0.0 0.0 0.0 2.687871993954088 0.0 86-87 0.0 0.0 0.0 3.1195558015579783 0.0 88-89 0.0 0.0 0.0 3.6585242820135493 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7800 0.0 48.8562 1 GTATCAA 14470 0.0 42.229153 1 TATCAAC 18585 0.0 32.668434 2 ATCAACG 18600 0.0 32.667633 3 TCAACGC 18830 0.0 32.296066 4 CAACGCA 19255 0.0 31.4838 5 AACGCAG 19755 0.0 30.73363 6 ACGCAGA 22220 0.0 27.066353 7 CGCAGAG 22215 0.0 26.857983 8 GTACATG 14185 0.0 25.993952 1 GCAGAGT 22990 0.0 25.29196 9 TACATGG 14310 0.0 25.098177 2 GAGTACT 12670 0.0 23.844906 12-13 ACATGGG 14535 0.0 23.66375 3 AGTACTT 12915 0.0 22.951195 12-13 GTACTTT 13375 0.0 22.356153 14-15 ATGGGAG 3610 0.0 22.109663 5 AGAGTAC 21855 0.0 21.017971 10-11 CATGGGA 9505 0.0 20.893642 4 CAGAGTA 22895 0.0 20.509314 10-11 >>END_MODULE