FastQCFastQC Report
Thu 26 May 2016
SRR937420_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937420_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180647
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185040.8485554975197728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147190.6749831586680467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105620.4843516626028881No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34330.15743034062826308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.11556203273615583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90350.046.297091
GTATCAA154950.039.3274351
ATCAACG200900.030.2156073
TATCAAC201000.030.153312
TCAACGC203450.029.8135474
CAACGCA207100.029.3110355
AACGCAG212250.028.6669736
ACCGTAT700.00839349827.1426548
ACGCAGA239650.025.2512197
CGCAGAG241150.025.0941528
GTACATG142300.024.2510681
GCAGAGT251000.023.8065959
GAGTACT143600.023.56789612-13
TACATGG144650.023.4783732
AGTACTT146750.023.09437612-13
GTACTTT151950.022.19412414-15
ACATGGG146000.022.0900823
AGAGTAC237050.020.78923610-11
TACTTTT162750.020.57541314-15
CAGAGTA248700.019.99683410-11
Produced by FastQC (version 0.11.5)