##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937420_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2180647 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23283273267062 33.0 31.0 34.0 31.0 34.0 2 32.42344221692002 34.0 31.0 34.0 31.0 34.0 3 32.459895159555856 34.0 31.0 34.0 31.0 34.0 4 36.1156404498298 37.0 35.0 37.0 35.0 37.0 5 36.04806692692581 37.0 35.0 37.0 35.0 37.0 6 36.09947368831361 37.0 35.0 37.0 35.0 37.0 7 36.042556177134585 37.0 35.0 37.0 35.0 37.0 8 36.02023757169317 37.0 35.0 37.0 35.0 37.0 9 37.721094244047755 39.0 37.0 39.0 35.0 39.0 10-11 37.64804895060961 39.0 37.0 39.0 35.0 39.0 12-13 37.54782273334473 39.0 37.0 39.0 35.0 39.0 14-15 38.71922553260569 40.0 38.0 41.0 34.5 41.0 16-17 38.70579213416936 40.0 38.0 41.0 34.5 41.0 18-19 38.7406895751582 40.0 38.0 41.0 35.0 41.0 20-21 38.67298054201345 40.0 38.0 41.0 34.0 41.0 22-23 38.52791373385972 40.0 38.0 41.0 34.0 41.0 24-25 38.45744611576289 40.0 38.0 41.0 34.0 41.0 26-27 38.3916883383693 40.0 38.0 41.0 34.0 41.0 28-29 38.24966351729556 40.0 38.0 41.0 34.0 41.0 30-31 38.25348715312474 40.0 37.5 41.0 34.0 41.0 32-33 38.12990823365726 40.0 37.0 41.0 34.0 41.0 34-35 37.976071092661954 40.0 37.0 41.0 33.5 41.0 36-37 37.78794045987269 40.0 37.0 41.0 33.0 41.0 38-39 37.63919148766398 40.0 37.0 41.0 33.0 41.0 40-41 37.41718627544944 40.0 36.0 41.0 33.0 41.0 42-43 37.153985720751685 40.0 36.0 41.0 32.5 41.0 44-45 36.7701780710037 40.0 36.0 41.0 31.0 41.0 46-47 36.27669838355314 39.5 35.5 41.0 30.0 41.0 48-49 35.8324699045742 39.0 35.0 41.0 28.5 41.0 50-51 35.540478124153054 39.0 35.0 41.0 27.5 41.0 52-53 35.26947277574041 39.0 35.0 40.5 27.0 41.0 54-55 35.0248758739952 39.0 35.0 40.0 26.0 41.0 56-57 34.761445341680705 38.0 34.5 40.0 26.0 41.0 58-59 34.49205877888535 38.0 34.0 40.0 25.0 41.0 60-61 34.169323370540944 38.0 34.0 40.0 24.5 41.0 62-63 33.796459032571526 37.0 33.5 40.0 23.0 41.0 64-65 33.40495412600022 36.5 33.0 39.5 22.0 41.0 66-67 33.00003416417238 36.0 33.0 39.0 21.5 40.5 68-69 32.579537174058885 35.5 32.0 39.0 20.0 40.0 70-71 32.183003943325076 35.0 32.0 38.0 18.5 40.0 72-73 31.730154628419914 35.0 31.5 37.5 17.0 39.5 74-75 31.27147883174122 35.0 31.0 37.0 13.0 39.0 76-77 30.01829915616787 33.5 29.5 35.5 10.5 38.0 78-79 30.607209236524756 34.0 31.0 36.0 7.5 38.5 80-81 30.419417493982287 34.0 31.0 36.0 7.0 37.0 82-83 30.098779628247947 34.0 30.5 35.0 2.0 37.0 84-85 29.758415965536834 34.0 30.0 35.0 2.0 36.5 86-87 29.357391407229137 34.0 30.0 35.0 2.0 36.0 88-89 28.92416952399907 34.0 29.0 35.0 2.0 36.0 90-91 28.44744312123879 34.0 29.0 35.0 2.0 35.0 92-93 28.128403863623962 34.0 29.0 35.0 2.0 35.0 94-95 27.72546244302723 33.5 27.5 35.0 2.0 35.0 96-97 27.28712304192288 33.0 27.0 35.0 2.0 35.0 98-99 26.601434803523908 33.0 25.5 35.0 2.0 35.0 100-101 24.239434672370173 31.0 19.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04383102348914747 1101 2 -0.040668660653807365 1101 3 -0.06466869906967077 1101 4 -0.07153396545835733 1101 5 -0.02591052559994722 1101 6 -0.08924115993397663 1101 7 -0.04578111481555425 1101 8 -0.13800017566009615 1101 9 -0.06038255368243739 1101 10-11 -0.004529637962541244 1101 12-13 -0.06520694041758901 1101 14-15 -0.23892499908468778 1101 16-17 -0.17564967207589177 1101 18-19 -0.03694660112029169 1101 20-21 -0.10438389442125384 1101 22-23 -0.12084737183736394 1101 24-25 -0.11528254297241602 1101 26-27 -0.13893303392119094 1101 28-29 -0.28054951194686595 1101 30-31 -0.17899954991781897 1101 32-33 -0.07462112284505196 1101 34-35 -0.05878865742300121 1101 36-37 -0.1250155616888975 1101 38-39 -0.02986102773691357 1101 40-41 -0.009557663326042132 1101 42-43 -0.02998256328153559 1101 44-45 0.08464558246927822 1101 46-47 0.08953446662251707 1101 48-49 0.25739846141185296 1101 50-51 0.3450569122786078 1101 52-53 0.35504853390547453 1101 54-55 0.16551709090147426 1101 56-57 0.1765243738625344 1101 58-59 0.2309253274993992 1101 60-61 0.20412956560379314 1101 62-63 0.11445873107989968 1101 64-65 0.09155188384451662 1101 66-67 0.287459861774245 1101 68-69 0.2810950115894073 1101 70-71 0.1982170359635873 1101 72-73 0.14087243001619143 1101 74-75 0.15384486962848243 1101 76-77 0.2465401940492562 1101 78-79 0.022231772404271766 1101 80-81 0.10419124540615243 1101 82-83 0.011444985040842681 1101 84-85 0.10290902569220961 1101 86-87 0.05054715952570277 1101 88-89 0.03720152913848551 1101 90-91 7.302192177149891E-4 1101 92-93 0.010660903275283573 1101 94-95 0.19785851309861613 1101 96-97 0.14427730571746977 1101 98-99 0.21075806345550774 1101 100-101 0.7814593046079779 1102 1 -0.07470643213426342 1102 2 -0.07771915102794225 1102 3 -0.026528488390411553 1102 4 -0.034120234982516706 1102 5 -0.06695665709285947 1102 6 -0.05328038986495898 1102 7 -0.07302412244359147 1102 8 -0.08169795989547879 1102 9 -0.1635427425672873 1102 10-11 -0.07082095652411624 1102 12-13 -0.1563802059461068 1102 14-15 -0.1871632845914064 1102 16-17 -0.22414222565380726 1102 18-19 -0.1909604042441515 1102 20-21 -0.1281761210830723 1102 22-23 -0.07253643831030132 1102 24-25 -0.32977582303053055 1102 26-27 -0.3154677233509062 1102 28-29 -0.2567572852850404 1102 30-31 -0.15865810422221927 1102 32-33 -0.0724416822348104 1102 34-35 -0.04589363381239053 1102 36-37 -0.15098722896097172 1102 38-39 -0.17025332704675833 1102 40-41 -0.1702914083314866 1102 42-43 -0.16674246157430161 1102 44-45 -0.17216516943772575 1102 46-47 -0.1156961908420655 1102 48-49 -0.07169707073489207 1102 50-51 -0.1346016420257854 1102 52-53 -0.2186892067410966 1102 54-55 -0.18283016663576035 1102 56-57 -0.17436556438665463 1102 58-59 -0.04586363000150584 1102 60-61 -0.10480614089821216 1102 62-63 -0.1697766485060086 1102 64-65 -0.20812120006394963 1102 66-67 -0.07069487850908018 1102 68-69 -0.1947495216470685 1102 70-71 -0.08002488194414781 1102 72-73 -0.06490308760095687 1102 74-75 -0.0762677348030465 1102 76-77 0.07944974726393284 1102 78-79 0.08761499071157175 1102 80-81 0.006479658046909975 1102 82-83 0.07410390258533894 1102 84-85 0.009737939604303136 1102 86-87 0.028207893270710827 1102 88-89 0.04210527051153079 1102 90-91 -0.1251324760238397 1102 92-93 0.028641288309788848 1102 94-95 0.054197052873409035 1102 96-97 0.32698707687620754 1102 98-99 0.3320802574257229 1102 100-101 0.8617353670852772 1103 1 -0.04548686573441785 1103 2 0.014997849138573827 1103 3 -0.03398872705791689 1103 4 -0.075737070080244 1103 5 -0.06094408128242179 1103 6 -0.03876424443662074 1103 7 -0.13310048447585388 1103 8 -0.13678231192137957 1103 9 -0.10929253986441978 1103 10-11 -0.11911528151676265 1103 12-13 -0.020426609045827604 1103 14-15 -0.11669098225766561 1103 16-17 -0.23648508708379268 1103 18-19 -0.22214786720604707 1103 20-21 -0.19043299755323773 1103 22-23 -0.08918871330010347 1103 24-25 -0.12063478636304126 1103 26-27 -0.24871129423735283 1103 28-29 -0.31942887399587505 1103 30-31 -0.3881519691584643 1103 32-33 -0.3507745175507395 1103 34-35 -0.30005503812697754 1103 36-37 -0.369632499444549 1103 38-39 -0.2311832388883701 1103 40-41 -0.34402526888079166 1103 42-43 -0.24758113815473592 1103 44-45 -0.40761369507951883 1103 46-47 -0.09040955402590356 1103 48-49 -0.21769954683828985 1103 50-51 -0.16847861373443607 1103 52-53 -0.11619550688794789 1103 54-55 -0.26475469774175053 1103 56-57 -0.28789327293436173 1103 58-59 -0.25362107552874136 1103 60-61 -0.30318083831146936 1103 62-63 -0.2436045906756661 1103 64-65 -0.3614911361253874 1103 66-67 -0.29699754241777043 1103 68-69 -0.3040362552508995 1103 70-71 -0.2896664654762695 1103 72-73 -0.14926749831798602 1103 74-75 -0.1367220841701524 1103 76-77 -0.035306276945011206 1103 78-79 -0.19919907860700903 1103 80-81 -0.32892976595050527 1103 82-83 -0.2486647801903672 1103 84-85 -0.3805034153313862 1103 86-87 -0.3513023439578653 1103 88-89 -0.23343919232479493 1103 90-91 -0.29819404887209444 1103 92-93 -0.07363406658666705 1103 94-95 0.030090409473960023 1103 96-97 0.015111087075563745 1103 98-99 0.15168845064482994 1103 100-101 0.7239611311888225 1104 1 0.09535983340227006 1104 2 0.01714151717342105 1104 3 -0.00503444289258681 1104 4 0.01378935833753303 1104 5 -0.0830287424920968 1104 6 -0.029669353553551048 1104 7 -0.004585161104031954 1104 8 -0.017344160598810276 1104 9 0.011220864899648575 1104 10-11 0.02009499006010884 1104 12-13 -0.010945944956368692 1104 14-15 -0.016880091951541942 1104 16-17 -0.02966876051511491 1104 18-19 -0.03069252612782236 1104 20-21 -0.001353689918225598 1104 22-23 -0.07658564875007556 1104 24-25 -0.09584497214346044 1104 26-27 -0.20724036369393417 1104 28-29 -0.055838528459965175 1104 30-31 -0.038234843563635934 1104 32-33 0.001673836711546528 1104 34-35 -0.09926622362264936 1104 36-37 -0.051634687254242806 1104 38-39 -0.05238613943037507 1104 40-41 -0.13203768512044434 1104 42-43 -0.09638803892553938 1104 44-45 -0.015699280777127456 1104 46-47 0.03999888685350328 1104 48-49 -0.07154481354715614 1104 50-51 -0.06914652051223413 1104 52-53 0.02820140928891135 1104 54-55 0.03544275845542444 1104 56-57 -0.028228814801167346 1104 58-59 -0.13285447795516347 1104 60-61 -0.0833859322585937 1104 62-63 0.03757923569717292 1104 64-65 -0.040285437872775276 1104 66-67 0.046946859611999514 1104 68-69 0.052556829554553985 1104 70-71 0.12020792975195604 1104 72-73 0.07850305894866594 1104 74-75 0.1172951951122414 1104 76-77 0.16265343042689295 1104 78-79 -0.07914379895407109 1104 80-81 -0.16385295014306678 1104 82-83 -0.025120087074352426 1104 84-85 0.020105894952127557 1104 86-87 -0.011368304648165406 1104 88-89 0.12963517766186072 1104 90-91 -0.01044858085241529 1104 92-93 0.23023911505686812 1104 94-95 0.32872184716148567 1104 96-97 0.27196773176259015 1104 98-99 0.3984770674442757 1104 100-101 0.9864072908025641 1105 1 0.01594612444647936 1105 2 -0.010063924953975345 1105 3 0.02291986967908599 1105 4 -0.0036026828888111595 1105 5 -0.03489372697897153 1105 6 -7.281826037797146E-5 1105 7 -0.028199336817316123 1105 8 -0.003939006266236333 1105 9 0.01017036833668783 1105 10-11 -0.030180385819924993 1105 12-13 -0.028191069262696544 1105 14-15 -0.08107750998002672 1105 16-17 -0.05739490542811865 1105 18-19 -3.197662455889372E-4 1105 20-21 -0.006384660978994816 1105 22-23 -0.1296052865909374 1105 24-25 -0.16689553688885184 1105 26-27 -0.11392734006903993 1105 28-29 0.011667280034394878 1105 30-31 -0.014087870798952906 1105 32-33 0.022990345433051118 1105 34-35 -0.003370465479385132 1105 36-37 0.060702941139780364 1105 38-39 -0.019417089711744495 1105 40-41 -0.007526569434979535 1105 42-43 0.08752613302148404 1105 44-45 0.029667121904459748 1105 46-47 -0.08266683867906721 1105 48-49 -0.04642429297098971 1105 50-51 -0.022382134342493032 1105 52-53 -0.19314792440632544 1105 54-55 -0.007455034121910842 1105 56-57 -0.04520940748188451 1105 58-59 -0.11610692310726023 1105 60-61 -0.04991314419428505 1105 62-63 0.07005363806187148 1105 64-65 0.0404793385602602 1105 66-67 0.001168115519512014 1105 68-69 0.02740335413334094 1105 70-71 0.0922513444323414 1105 72-73 0.20030944225345237 1105 74-75 0.286202818298424 1105 76-77 0.34439250143143596 1105 78-79 0.17861089816654996 1105 80-81 0.10563545754375525 1105 82-83 0.09497824498840757 1105 84-85 0.08927129645577736 1105 86-87 -0.010069672712550215 1105 88-89 0.031160662309126508 1105 90-91 0.20346537680694254 1105 92-93 0.38713908038122824 1105 94-95 0.5200316220555443 1105 96-97 0.4259602842922874 1105 98-99 0.4861638223887965 1105 100-101 1.142385900348362 1106 1 -0.17453564890534068 1106 2 -0.10200920412837178 1106 3 -0.11428434146598931 1106 4 -0.12263355330954795 1106 5 -0.15704290357685124 1106 6 -0.14496482923738796 1106 7 -0.0855808528886115 1106 8 -0.1552936460140799 1106 9 -0.12455036361792793 1106 10-11 -0.2160752865654061 1106 12-13 -0.2834257355346068 1106 14-15 -0.250013435496534 1106 16-17 -0.2382185740833691 1106 18-19 -0.21396020816440853 1106 20-21 -0.08007159564530042 1106 22-23 -0.15357878486043575 1106 24-25 -0.12605509079187271 1106 26-27 -0.2630418959945118 1106 28-29 -0.2101794487786819 1106 30-31 -0.22964476984650872 1106 32-33 -0.14427161785900466 1106 34-35 -0.09563297805436832 1106 36-37 -0.22043941966742153 1106 38-39 -0.2045907189446936 1106 40-41 -0.15560505169961658 1106 42-43 -0.22992995225892798 1106 44-45 -0.06722278414834904 1106 46-47 -0.26422087353134316 1106 48-49 -0.3317637156007933 1106 50-51 -0.17506072603149647 1106 52-53 -0.11692366281366873 1106 54-55 -0.13877570477294654 1106 56-57 -0.3312985562806503 1106 58-59 -0.43437918878586856 1106 60-61 -0.254462413431888 1106 62-63 -0.11515835092942694 1106 64-65 -0.20860822146015323 1106 66-67 -0.1419258493198967 1106 68-69 -0.2474589019175042 1106 70-71 -0.19963670126222866 1106 72-73 -0.3946045760591055 1106 74-75 -0.24886430407496718 1106 76-77 -0.13461498574505626 1106 78-79 -0.21669361528223874 1106 80-81 -0.2813199554503676 1106 82-83 -0.36710538504775414 1106 84-85 -0.4228271552147085 1106 86-87 -0.3205702153320438 1106 88-89 -0.07118758522621604 1106 90-91 -0.26085842709797546 1106 92-93 -0.12318806892683654 1106 94-95 -0.1277570009083746 1106 96-97 -0.10277990920658198 1106 98-99 -0.14322013069775608 1106 100-101 0.28517226674997076 1107 1 0.00406874572881577 1107 2 -0.031654466360500066 1107 3 -0.005959005986134969 1107 4 -0.034811962201096947 1107 5 -0.03371026969737301 1107 6 -0.04879852433742826 1107 7 -0.020437619645981897 1107 8 0.03534422150018912 1107 9 0.029743996376069504 1107 10-11 0.018384798125843815 1107 12-13 0.06089934309662226 1107 14-15 -0.012119720376063015 1107 16-17 0.011037252883213 1107 18-19 -0.06722224307060287 1107 20-21 0.009772678407593105 1107 22-23 0.08334085477176956 1107 24-25 0.07115563630760136 1107 26-27 0.00674060825667766 1107 28-29 -0.05322143091623133 1107 30-31 0.026080431934452974 1107 32-33 0.019535027869920896 1107 34-35 -0.14050609597885 1107 36-37 -0.0958325757981342 1107 38-39 -0.082864248973614 1107 40-41 -0.17214799529956082 1107 42-43 -0.28118816654177436 1107 44-45 -0.2674001702319728 1107 46-47 -0.4980024306532158 1107 48-49 -0.3169550735617932 1107 50-51 -0.3131991353358359 1107 52-53 -0.42822534643348575 1107 54-55 -0.28459492003159426 1107 56-57 -0.3122496185905206 1107 58-59 -0.3409977995329143 1107 60-61 -0.18405865656205123 1107 62-63 -0.22290491701232895 1107 64-65 -0.2814311140114327 1107 66-67 -0.2090705525622596 1107 68-69 -0.195091021297074 1107 70-71 -0.1511851888939333 1107 72-73 -0.005413832700188692 1107 74-75 -0.151977905700452 1107 76-77 0.015546863289866764 1107 78-79 -0.012162415627148704 1107 80-81 -0.14569184015743986 1107 82-83 -0.2632926735632921 1107 84-85 -0.1627610396259449 1107 86-87 0.09323041050716085 1107 88-89 -0.012103514313061225 1107 90-91 0.09747292827159626 1107 92-93 0.3256024492308711 1107 94-95 0.1909348139909106 1107 96-97 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9 0.16299561061102708 2316 10-11 0.07880320556482445 2316 12-13 0.048259745527843734 2316 14-15 -0.1432576658900686 2316 16-17 0.060482255788620876 2316 18-19 -0.0700449451451064 2316 20-21 0.04770066097795933 2316 22-23 0.19755177770274912 2316 24-25 0.25187855342492327 2316 26-27 0.3145579307261741 2316 28-29 0.44145268943704963 2316 30-31 0.2791912008998665 2316 32-33 0.26091104741463766 2316 34-35 0.1958436334856941 2316 36-37 0.29363315378516575 2316 38-39 0.3080888247531206 2316 40-41 0.2523339437900134 2316 42-43 0.13200508498763242 2316 44-45 0.04101307957256495 2316 46-47 -0.03976470725115888 2316 48-49 -0.3930718894577083 2316 50-51 -0.3092948569593048 2316 52-53 0.03141277086027827 2316 54-55 0.13136801512614227 2316 56-57 0.3659319476733529 2316 58-59 0.3841213505884582 2316 60-61 0.42530850112612484 2316 62-63 0.3236184160523763 2316 64-65 0.2814621944951341 2316 66-67 0.3920377450366388 2316 68-69 0.4190506241975598 2316 70-71 0.4571088754435948 2316 72-73 0.3551278602989676 2316 74-75 0.40684924144058243 2316 76-77 0.21449610837788313 2316 78-79 0.10868076146435968 2316 80-81 0.11015962901904786 2316 82-83 0.12300750132688876 2316 84-85 0.18723068949644883 2316 86-87 0.06195023417487633 2316 88-89 0.14156585700127167 2316 90-91 0.32557737036909273 2316 92-93 0.371938450452415 2316 94-95 0.15653573919814434 2316 96-97 0.42924915754842274 2316 98-99 0.632325826543024 2316 100-101 0.411309412228146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 25.0 5 63.0 6 104.0 7 267.0 8 639.0 9 1166.0 10 2091.0 11 3546.0 12 5820.0 13 8171.0 14 10692.0 15 15301.0 16 21560.0 17 31962.0 18 28041.0 19 15214.0 20 12023.0 21 11804.0 22 11833.0 23 12633.0 24 14057.0 25 16709.0 26 19763.0 27 24516.0 28 30389.0 29 37888.0 30 47480.0 31 59449.0 32 76540.0 33 100978.0 34 139913.0 35 203331.0 36 311187.0 37 473516.0 38 393353.0 39 38614.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75045029872301 19.685476954112897 15.400927626841979 21.163145120322113 2 15.579733904662241 21.34884738336833 39.86326076618545 23.20815794578398 3 18.759890986482453 26.394184845139996 30.713774398148807 24.132149770228743 4 12.63849673972908 17.55511093725853 37.248348769883435 32.558043553128954 5 12.917097357340115 38.738038875431876 33.42960626541429 14.915257501813716 6 30.485585241444397 37.4026149119963 17.28124726285364 14.83055258370566 7 27.635421964215208 34.790454392664195 20.203361662845936 17.37076198027466 8 27.0886117743954 34.4506469868805 19.309039931726684 19.151701306997417 9 27.674859800783896 16.17400707221297 20.119946052708208 36.03118707429493 10-11 24.910295933423292 27.611105442547323 26.423175738343176 21.055422885686216 12-13 26.4082801811668 25.106510704727103 27.650362388628096 20.834846725478005 14-15 23.027638127583234 25.75423715988879 27.067608833525096 24.15051587900288 16-17 21.330962783063924 29.42156616820604 27.360343054148608 21.887127994581423 18-19 21.200863780336753 26.715626142149556 31.52525374349906 20.55825633401463 20-21 22.312758552851516 25.706430247536627 31.73122472367146 20.249586475940397 22-23 21.909868769666037 25.859481530923496 31.527243369638562 20.703406329771905 24-25 21.580871604912794 26.06330961315123 31.777248114939127 20.578570666996846 26-27 21.339663900883984 26.31937968570186 31.608340042001497 20.73261637141266 28-29 20.99705124324721 26.408868282750458 31.871772647644203 20.722307826358126 30-31 21.232185542071985 26.209376845073972 31.774400834618316 20.784036778235723 32-33 21.16640153128865 26.288573987445012 31.539607281692085 21.005417199574257 34-35 21.466518881781415 26.32156878210916 31.36537000257263 20.846542333536792 36-37 21.682652369377614 26.067063139302952 31.299937885393163 20.95034660592627 38-39 21.4311984496252 26.280590874030214 31.269782090896502 21.018428585448085 40-41 21.494557802851908 26.404137307998322 31.182585886285942 20.91871900286383 42-43 21.479257103676645 26.493050539239633 30.760484960224915 21.26720739685881 44-45 21.48566305572784 26.740238255468284 30.469918842726397 21.304179846077474 46-47 21.75386479333886 27.089322572612623 29.721706447673558 21.43510618637496 48-49 22.016195193444883 27.236205584856236 29.194156596643104 21.553442625055773 50-51 22.107704731669088 27.512247511862302 28.828485307342273 21.551562449126337 52-53 22.2591964678373 27.676739976713332 28.606991411264637 21.45707214418473 54-55 21.761454283980854 27.671856105091745 28.71118067252517 21.855508938402227 56-57 21.959308407092024 27.948723475188785 28.46556549501134 21.626402622707847 58-59 22.137604114742093 27.45166916057482 28.6473464068233 21.76338031785979 60-61 22.060058322140172 27.490464985850533 29.01634239746277 21.433134294546527 62-63 22.028989561354955 27.545907246794187 29.041724772510175 21.38337841934068 64-65 21.82677893304143 27.525087737721876 28.99747185124415 21.650661477992543 66-67 21.819991956515658 27.861066004722453 28.60025029268836 21.718691746073528 68-69 21.861332897988532 27.72518890035847 28.79159258697075 21.62188561468225 70-71 21.769662856941082 27.904814488543995 28.606739192542396 21.718783461972524 72-73 21.977651586891415 27.87525904009223 28.29671652495796 21.8503728480584 74-75 21.767163598693415 28.194178149879374 28.08212425027985 21.956534001147364 76-77 21.84374637435587 27.915361816928648 28.362155818892283 21.878735989823202 78-79 21.8141909259041 27.88940621751251 28.283004998057915 22.013397858525472 80-81 21.7135067835434 28.420352693813065 27.98568772083489 21.88045280180864 82-83 21.733433059499102 28.395244877875193 27.90215524080567 21.969166821820036 84-85 21.73403019584698 28.082055148257545 27.887157432871497 22.296757223023977 86-87 21.682922545464717 27.895459466846305 27.909629573241336 22.51198841444764 88-89 21.7134180818812 27.843250191342296 27.924877341449577 22.518454385326926 90-91 21.642276187862063 28.024434276872125 28.001847315350442 22.33144221991537 92-93 21.538047393747455 27.672530694772913 28.12566639288667 22.66375551859296 94-95 21.33951529064539 27.670342792758294 28.12756947823284 22.862572438363475 96-97 21.535947817322107 27.657754785620963 28.0190007827952 22.78729661426173 98-99 21.270877151629783 27.61602251812366 27.890987224642632 23.22211310560392 100-101 20.9357547728387 27.912008332374032 28.12045527196478 23.031781622822486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1304.0 1 912.5 2 633.0 3 776.5 4 705.5 5 712.5 6 1279.5 7 2862.0 8 4641.0 9 5498.0 10 5118.0 11 4513.0 12 4671.0 13 5008.0 14 5330.0 15 5557.0 16 5740.5 17 5961.5 18 6246.0 19 6531.0 20 6982.5 21 7757.5 22 8684.0 23 9841.0 24 11437.5 25 13733.5 26 16743.0 27 20033.5 28 22804.0 29 25743.0 30 29563.0 31 33240.0 32 36768.5 33 40001.5 34 44869.0 35 48544.0 36 51763.0 37 56486.5 38 59511.0 39 60586.5 40 61058.5 41 64497.5 42 68462.5 43 71680.5 44 74212.5 45 76131.5 46 75564.5 47 76126.5 48 82585.0 49 82401.5 50 76813.5 51 73313.0 52 70327.5 53 68685.0 54 66475.5 55 61881.5 56 55378.5 57 49129.5 58 45384.0 59 41445.0 60 36002.0 61 29901.0 62 24299.5 63 18889.0 64 13395.0 65 10157.5 66 7540.5 67 5820.5 68 4270.0 69 2597.5 70 1833.0 71 1404.5 72 1157.0 73 966.5 74 764.0 75 570.5 76 382.5 77 237.0 78 158.5 79 108.0 80 57.0 81 41.5 82 35.5 83 20.0 84 13.0 85 13.0 86 8.0 87 4.0 88 2.0 89 1.0 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07052952632865384 2 0.0 3 0.0 4 0.0 5 0.0018801759294374558 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.0636077274313543E-4 12-13 0.005273664192324572 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.107982172263553E-4 24-25 6.649402677278807E-4 26-27 4.8150846973398257E-4 28-29 0.003072482616397794 30-31 4.356505202355081E-4 32-33 0.0 34-35 0.0 36-37 2.2928974749237267E-5 38-39 5.96153343480169E-4 40-41 9.171589899694907E-5 42-43 0.012014782768600329 44-45 2.0636077274313543E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.171589899694907E-5 82-83 4.1272154548627085E-4 84-85 7.337271919755925E-4 86-87 0.0 88-89 0.0 90-91 0.008506649631967025 92-93 0.00293490876790237 94-95 0.0 96-97 0.0 98-99 1.6050282324466087E-4 100-101 0.01082247608163999 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2180647.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.6779980109976 #Duplication Level Percentage of deduplicated Percentage of total 1 69.82010736643917 31.194226180454088 2 15.03752082499092 13.436926510185588 3 5.826799436529534 7.809892009072453 4 2.9126719107136925 5.205293993342198 5 1.6583733425856686 3.7046400450766965 6 1.0232538708943832 2.743016064513891 7 0.6809198764710336 2.1295495820635097 8 0.5171433853957699 1.8483944915290218 9 0.382533296967783 1.5381739692960266 >10 1.9114299311035292 15.509524184713946 >50 0.14638742780164404 4.486514668681041 >100 0.07711155640720845 6.267730016352267 >500 0.004002913826551341 1.1734798162436129 >1k 0.0014369434249158588 0.9447911158291152 >5k 0.0 0.0 >10k+ 3.079164481962555E-4 2.007847352646596 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18504 0.8485554975197728 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14719 0.6749831586680467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10562 0.4843516626028881 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3433 0.15743034062826308 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2520 0.11556203273615583 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.87869242477118E-5 0.0 22-23 0.0 0.0 0.0 2.2928974749237267E-4 0.0 24-25 0.0 0.0 0.0 3.897925707370335E-4 0.0 26-27 0.0 0.0 0.0 7.337271919755925E-4 0.0 28-29 0.0 0.0 0.0 0.0019031049041866933 0.0 30-31 0.0 0.0 0.0 0.005296593167073808 0.0 32-33 0.0 0.0 0.0 0.009332092722939568 0.0 34-35 0.0 0.0 0.0 0.013963745622285497 0.0 36-37 0.0 0.0 0.0 0.016669364642695493 0.0 38-39 0.0 0.0 0.0 0.01914569391561312 0.0 40-41 0.0 0.0 0.0 0.0233187673199743 0.0 42-43 0.0 0.0 0.0 0.027927491244570994 0.0 44-45 0.0 0.0 0.0 0.033040652613650905 0.0 46-47 0.0 0.0 0.0 0.04076771710414386 0.0 48-49 0.0 0.0 0.0 0.051292116514043765 0.0 50-51 0.0 0.0 0.0 0.06578322855556172 0.0 52-53 0.0 0.0 0.0 0.09884681014396186 0.0 54-55 0.0 0.0 0.0 0.14071511803606912 0.0 56-57 0.0 0.0 0.0 0.19182380275211897 0.0 58-59 0.0 0.0 0.0 0.2620552524090327 0.0 60-61 0.0 0.0 0.0 0.36175043461871637 0.0 62-63 0.0 0.0 0.0 0.4334493386595813 0.0 64-65 0.0 0.0 0.0 0.5071659924783791 0.0 66-67 0.0 0.0 0.0 0.6423322986251327 0.0 68-69 0.0 0.0 0.0 0.8125111492139718 0.0 70-71 0.0 0.0 0.0 0.9573764116796528 0.0 72-73 0.0 0.0 0.0 1.111344477120781 0.0 74-75 0.0 0.0 0.0 1.3683324261102325 0.0 76-77 0.0 0.0 0.0 1.570176190827768 0.0 78-79 0.0 0.0 0.0 1.7536079888216662 0.0 80-81 0.0 0.0 0.0 1.9841359009504977 0.0 82-83 0.0 0.0 0.0 2.3489817471603613 0.0 84-85 0.0 0.0 0.0 2.7142403149157106 0.0 86-87 0.0 0.0 0.0 3.153284323414106 0.0 88-89 0.0 0.0 0.0 3.7007135955521457 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9035 0.0 46.29709 1 GTATCAA 15495 0.0 39.327435 1 ATCAACG 20090 0.0 30.215607 3 TATCAAC 20100 0.0 30.15331 2 TCAACGC 20345 0.0 29.813547 4 CAACGCA 20710 0.0 29.311035 5 AACGCAG 21225 0.0 28.666973 6 ACCGTAT 70 0.008393498 27.142654 8 ACGCAGA 23965 0.0 25.251219 7 CGCAGAG 24115 0.0 25.094152 8 GTACATG 14230 0.0 24.251068 1 GCAGAGT 25100 0.0 23.806595 9 GAGTACT 14360 0.0 23.567896 12-13 TACATGG 14465 0.0 23.478373 2 AGTACTT 14675 0.0 23.094376 12-13 GTACTTT 15195 0.0 22.194124 14-15 ACATGGG 14600 0.0 22.090082 3 AGAGTAC 23705 0.0 20.789236 10-11 TACTTTT 16275 0.0 20.575413 14-15 CAGAGTA 24870 0.0 19.996834 10-11 >>END_MODULE