FastQCFastQC Report
Thu 26 May 2016
SRR937419_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937419_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427780
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69851.6328486605264387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50951.1910327738557203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37990.8880733087100846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11720.273972602739726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11340.2650895320024312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.22815465893683667No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.18420683528916731No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA7730.1807003599981299No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6790.1587264481742952No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA6150.1437654869325354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5950.1390901865444855No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5810.13581747627285054No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.12810323063256815No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT5430.12693440553555566No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.12085651503109074No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4830.11290850437140586No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG4370.10215531347889102No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4300.10051895834307355No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG500.001612744138.0092749
GTATCAA54400.035.1887661
GGTATCA34500.032.6307561
TAGGGTG650.005836392829.2344785
TACACGG650.005836392829.2344784
TATCAAC68150.028.0923422
GTACACG856.3957873E-427.9447253
ATCAACG68950.027.7663963
TCAACGC69000.027.7462754
CAACGCA69800.027.4282675
AACGCAG70750.027.1239416
GGACACG1606.221853E-826.7190136
GTACATG31050.026.159681
CACGGTG1109.4472736E-525.9154159
AGTACTT45500.024.89859812-13
TACATGG32200.024.7857552
ACGCAGA76600.024.7482057
GAGTACT44550.024.6298712-13
CGCAGAG77050.024.6036688
TTAGGGT2151.6516424E-924.305414
Produced by FastQC (version 0.11.5)