##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937419_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 427780 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.894716910561502 33.0 31.0 34.0 30.0 34.0 2 31.969727429987376 33.0 31.0 34.0 30.0 34.0 3 31.9566108747487 33.0 31.0 34.0 30.0 34.0 4 35.62040534854364 37.0 35.0 37.0 33.0 37.0 5 35.64688624994156 37.0 35.0 37.0 35.0 37.0 6 35.71841600822853 37.0 35.0 37.0 35.0 37.0 7 35.66865678619851 37.0 35.0 37.0 33.0 37.0 8 35.661625134414884 37.0 35.0 37.0 33.0 37.0 9 37.28079620365609 39.0 37.0 39.0 34.0 39.0 10-11 37.17253144139511 39.0 37.0 39.0 34.0 39.0 12-13 37.02117326663238 39.0 37.0 39.0 33.0 39.0 14-15 38.04657884894104 40.0 38.0 41.0 33.0 41.0 16-17 38.02599934545795 40.0 38.0 41.0 33.0 41.0 18-19 38.08006568797045 40.0 38.0 41.0 33.5 41.0 20-21 38.04114614989013 40.0 38.0 41.0 33.0 41.0 22-23 38.06503693487306 40.0 38.0 41.0 33.5 41.0 24-25 38.00835943709383 40.0 38.0 41.0 33.0 41.0 26-27 37.894414184861375 40.0 37.5 41.0 33.0 41.0 28-29 37.72681635420076 40.0 37.0 41.0 33.0 41.0 30-31 37.34666884847351 39.0 36.5 41.0 33.0 41.0 32-33 36.953379073355464 39.0 35.0 41.0 32.0 41.0 34-35 36.83105217635233 39.0 35.0 41.0 31.5 41.0 36-37 36.61674108186451 39.0 35.0 41.0 31.0 41.0 38-39 36.4045747814297 39.0 35.0 41.0 31.0 41.0 40-41 36.06955093739773 39.0 35.0 41.0 30.0 41.0 42-43 35.660616438356165 39.0 35.0 40.0 29.5 41.0 44-45 35.168240684463974 38.5 35.0 40.0 26.0 41.0 46-47 34.4372457805414 38.0 34.5 40.0 21.5 41.0 48-49 33.84427275702464 38.0 34.0 40.0 15.0 41.0 50-51 32.76757796063397 37.5 32.0 39.5 8.5 40.5 52-53 32.821452615830566 37.5 33.0 39.5 6.0 40.5 54-55 33.07098508579176 38.0 33.0 40.0 2.0 41.0 56-57 32.79630183739305 38.0 33.0 40.0 2.0 41.0 58-59 32.52518701201552 37.5 33.0 40.0 2.0 41.0 60-61 32.171721445602884 37.0 32.0 40.0 2.0 41.0 62-63 31.789560054233483 36.0 31.5 40.0 2.0 41.0 64-65 31.34559820468465 36.0 31.0 39.0 2.0 41.0 66-67 30.895190284725793 35.0 30.5 39.0 2.0 40.5 68-69 30.41188227593623 35.0 30.0 38.5 2.0 40.0 70-71 29.922905465426155 35.0 29.0 38.0 2.0 40.0 72-73 29.444165225115714 35.0 29.0 37.0 2.0 39.5 74-75 28.925464023563514 34.5 28.0 37.0 2.0 39.0 76-77 28.410143064191875 34.0 27.0 36.0 2.0 39.0 78-79 27.9066120435738 34.0 26.0 36.0 2.0 38.0 80-81 27.350883631773343 34.0 26.0 35.0 2.0 37.0 82-83 26.834247744167563 33.5 25.0 35.0 2.0 37.0 84-85 26.335521763523303 33.0 23.5 35.0 2.0 36.0 86-87 25.78275398569358 33.0 21.0 35.0 2.0 36.0 88-89 25.251335967085886 32.0 19.0 35.0 2.0 35.0 90-91 24.710838515124596 32.0 14.0 35.0 2.0 35.0 92-93 24.089802001028566 31.5 4.5 34.0 2.0 35.0 94-95 23.359606573472348 31.0 2.0 34.0 2.0 35.0 96-97 22.549192341857967 31.0 2.0 34.0 2.0 35.0 98-99 21.426922717284583 30.0 2.0 34.0 2.0 35.0 100-101 18.583318528215436 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1985762225605292 1101 2 0.28381867807823014 1101 3 0.13868220126442665 1101 4 0.11220170699749588 1101 5 0.12225341964911962 1101 6 0.2578537453925023 1101 7 0.18410609058238947 1101 8 0.0500743455846262 1101 9 0.1030231851957879 1101 10-11 0.1434078855626808 1101 12-13 0.22594856167834365 1101 14-15 0.5405738392321311 1101 16-17 0.3952507788684798 1101 18-19 0.4770940631593632 1101 20-21 0.3254870987418528 1101 22-23 0.318627075061535 1101 24-25 0.2718030139797065 1101 26-27 0.3943052196082135 1101 28-29 0.4291201892151477 1101 30-31 0.27634397139733125 1101 32-33 0.39261439897187955 1101 34-35 0.6152894913277933 1101 36-37 0.5727330483050466 1101 38-39 0.7351951374241921 1101 40-41 0.6556796014356863 1101 42-43 0.8367138308086339 1101 44-45 1.1573532748630555 1101 46-47 1.3953592610622252 1101 48-49 1.5867152259374393 1101 50-51 1.5341379482832949 1101 52-53 1.637763685677747 1101 54-55 1.7307781235644981 1101 56-57 1.9475200138577193 1101 58-59 1.8984619473368802 1101 60-61 2.040986737750039 1101 62-63 2.0675168871603873 1101 64-65 2.00294317221568 1101 66-67 2.027272642559147 1101 68-69 2.001802120855885 1101 70-71 1.8812329783329922 1101 72-73 2.0482339117267117 1101 74-75 2.23678399249901 1101 76-77 2.2939881622564933 1101 78-79 2.2330627372958 1101 80-81 2.1623146167485423 1101 82-83 2.176120006165256 1101 84-85 2.4839137102500004 1101 86-87 2.5592373904652987 1101 88-89 2.0965198076561293 1101 90-91 2.055716236808049 1101 92-93 2.0651469188447287 1101 94-95 2.126753643938258 1101 96-97 2.562883370776774 1101 98-99 2.817951060790545 1101 100-101 2.7025915079312774 1102 1 0.26074683540393195 1102 2 0.28333564879457995 1102 3 0.22910140167845228 1102 4 0.2689662733493279 1102 5 0.2714167439256485 1102 6 0.15408654825617418 1102 7 0.1726858982332331 1102 8 0.16729951486050965 1102 9 0.5165242849499592 1102 10-11 0.2618815992815797 1102 12-13 0.19505732952239185 1102 14-15 0.4927151252976003 1102 16-17 0.5073804204780075 1102 18-19 0.3469683461627753 1102 20-21 0.37528655533390776 1102 22-23 0.32444175570154954 1102 24-25 0.28123502329526673 1102 26-27 0.28442468310068847 1102 28-29 0.30908417765694196 1102 30-31 0.49903233125414914 1102 32-33 0.21830983175748742 1102 34-35 0.4945009028946217 1102 36-37 0.8526409708478511 1102 38-39 0.5905742291566298 1102 40-41 1.0000934971531095 1102 42-43 1.1221841374459771 1102 44-45 1.2951869747811102 1102 46-47 1.264483123571388 1102 48-49 1.2618845018247953 1102 50-51 1.3220244795292473 1102 52-53 1.220207511959714 1102 54-55 1.5010707444296472 1102 56-57 1.5781309165186954 1102 58-59 1.4843721556432605 1102 60-61 1.7008263030236748 1102 62-63 1.5042303472889103 1102 64-65 1.4234913241973999 1102 66-67 1.6751011671634508 1102 68-69 1.9135694905026668 1102 70-71 1.64130111996408 1102 72-73 1.6513143016003475 1102 74-75 1.381418917241323 1102 76-77 1.4955235053366707 1102 78-79 1.4697621809495693 1102 80-81 1.3058443963664352 1102 82-83 1.4698395472874353 1102 84-85 1.7393445551199704 1102 86-87 1.8821866723905138 1102 88-89 1.7353810229923106 1102 90-91 1.9044978816952707 1102 92-93 2.0563348689892074 1102 94-95 2.2347042196919134 1102 96-97 2.109416988752365 1102 98-99 2.133071602473386 1102 100-101 1.914437585474726 1103 1 0.24394501813459257 1103 2 0.3691118182655124 1103 3 0.31264560517035633 1103 4 0.1623258443892368 1103 5 0.21209663510079935 1103 6 0.12163983809478651 1103 7 0.22613686101618669 1103 8 0.2607462514356982 1103 9 0.14854837433425416 1103 10-11 0.2777206335931055 1103 12-13 0.18119800941357056 1103 14-15 0.32551313897995016 1103 16-17 0.23540678144897953 1103 18-19 0.06757507111588978 1103 20-21 0.03355407428581003 1103 22-23 0.09552921178860885 1103 24-25 0.09817683369702479 1103 26-27 -0.1303023302911086 1103 28-29 -0.30199773153241694 1103 30-31 -0.09197509102325085 1103 32-33 0.26430814127439106 1103 34-35 0.6044470187455389 1103 36-37 0.6162520012501318 1103 38-39 0.7809622478576017 1103 40-41 0.819724232249122 1103 42-43 1.0071877033087802 1103 44-45 1.0442123056139394 1103 46-47 0.5031119832877096 1103 48-49 0.6357837067694518 1103 50-51 1.110035256359069 1103 52-53 0.7257081855115786 1103 54-55 0.8745209147986444 1103 56-57 0.9683961110563217 1103 58-59 1.2262433091338387 1103 60-61 1.034256927181744 1103 62-63 1.218448895058259 1103 64-65 1.1227504171475573 1103 66-67 1.459818143311793 1103 68-69 1.5391459716564242 1103 70-71 1.5507440529522718 1103 72-73 1.5037668227974201 1103 74-75 1.342080847033447 1103 76-77 1.507059731861407 1103 78-79 1.3272214376652833 1103 80-81 1.6539109063112285 1103 82-83 1.8509655675204044 1103 84-85 1.4664147365827702 1103 86-87 1.8774665265863497 1103 88-89 1.852445657557407 1103 90-91 1.8782584141974503 1103 92-93 1.9764106765910974 1103 94-95 2.11305943245506 1103 96-97 2.2521122527582804 1103 98-99 2.3825855885797296 1103 100-101 2.397773119957943 1104 1 0.30919379513017375 1104 2 0.43376997361690073 1104 3 0.24478974071503856 1104 4 0.19597338048278345 1104 5 0.2627170861209649 1104 6 0.46891939900637425 1104 7 0.5352944794388108 1104 8 0.3663124129915971 1104 9 0.26108864399181186 1104 10-11 0.5083017098369851 1104 12-13 0.3909641471059686 1104 14-15 0.6522044645974177 1104 16-17 0.6676791833103337 1104 18-19 0.7467511837516128 1104 20-21 0.8171001905010584 1104 22-23 0.8263369912898568 1104 24-25 0.8687054102392011 1104 26-27 0.8112738539351199 1104 28-29 0.7570375864487318 1104 30-31 0.8790665507856019 1104 32-33 0.8951129378380429 1104 34-35 0.8871042195568961 1104 36-37 1.0747981174653845 1104 38-39 0.9018666745369899 1104 40-41 1.0054896540365021 1104 42-43 0.9904981674538718 1104 44-45 0.8284325657260965 1104 46-47 0.85234835126289 1104 48-49 0.9540929777395064 1104 50-51 0.9081589886105874 1104 52-53 0.8655154877428188 1104 54-55 0.9508930660932364 1104 56-57 1.248070374270732 1104 58-59 1.4422229058406835 1104 60-61 1.4297944525367114 1104 62-63 1.444991073795883 1104 64-65 1.4079938248554669 1104 66-67 1.738591559371926 1104 68-69 1.7806595141931147 1104 70-71 1.7585951447074315 1104 72-73 2.1480634461120616 1104 74-75 1.8808858822321106 1104 76-77 1.954795682225324 1104 78-79 2.0161428074302243 1104 80-81 2.003922838061616 1104 82-83 1.9977350461029104 1104 84-85 1.8171545051068136 1104 86-87 1.8554822764968577 1104 88-89 1.8142043975645628 1104 90-91 2.269455765348866 1104 92-93 2.5117376065517227 1104 94-95 2.5004386234823848 1104 96-97 2.339115593648394 1104 98-99 2.6126714971825216 1104 100-101 2.32112355546683 1105 1 -0.24421160696451238 1105 2 -0.31519186873013183 1105 3 -0.3238144119155706 1105 4 -0.34898282702624783 1105 5 -0.42324787988626156 1105 6 -0.2996143181492599 1105 7 -0.3908003328353331 1105 8 -0.22542213714008597 1105 9 -0.38706472159893224 1105 10-11 -0.20145819706569057 1105 12-13 -0.22640891591846923 1105 14-15 -0.08675554030244115 1105 16-17 -0.14627163435365986 1105 18-19 -0.2289011099949434 1105 20-21 -0.34153581121389465 1105 22-23 -0.06168897796352013 1105 24-25 -0.1394773545063117 1105 26-27 0.03647137950625989 1105 28-29 -0.0742954814755663 1105 30-31 0.016761779547771027 1105 32-33 -0.06517829753894233 1105 34-35 0.018640937876241992 1105 36-37 0.31132197365451475 1105 38-39 0.2819671216491315 1105 40-41 0.2618564084923136 1105 42-43 -0.031711975254530955 1105 44-45 0.07776802233171765 1105 46-47 0.014784806214805712 1105 48-49 0.18215079607722373 1105 50-51 0.18270268922919541 1105 52-53 0.2964688165846141 1105 54-55 0.5331019838368505 1105 56-57 0.6090123721882961 1105 58-59 0.7204506880419395 1105 60-61 0.41465082541598974 1105 62-63 0.3099968923785994 1105 64-65 0.39396032206867204 1105 66-67 0.5714267403604723 1105 68-69 0.43997384015624164 1105 70-71 0.4776494718955284 1105 72-73 0.4581357190342139 1105 74-75 0.22475952711481995 1105 76-77 0.1497748578240241 1105 78-79 0.36619486843347104 1105 80-81 0.4650975792878036 1105 82-83 0.7000823848669242 1105 84-85 0.7018179460328824 1105 86-87 1.0313412830504198 1105 88-89 1.1305974887631933 1105 90-91 1.3117247063668103 1105 92-93 1.0782363509628219 1105 94-95 0.46545822291890104 1105 96-97 0.3467578426837328 1105 98-99 0.7207317654498091 1105 100-101 0.3248168242853531 1106 1 -0.0032218224578564048 1106 2 -0.7292294790377234 1106 3 -0.08248630380238353 1106 4 -0.09311620171024515 1106 5 0.040175579096697334 1106 6 0.0787442484863945 1106 7 0.21517953691007818 1106 8 0.20297543068910073 1106 9 0.332845622645344 1106 10-11 -0.3497990017120003 1106 12-13 -0.2829183560868742 1106 14-15 -0.11125110920298198 1106 16-17 0.09704882388687253 1106 18-19 0.16092286775088382 1106 20-21 0.17859444337462804 1106 22-23 0.1633831507498158 1106 24-25 0.37950635897118445 1106 26-27 0.3037752222985546 1106 28-29 0.11983178458775967 1106 30-31 -0.03487251142932024 1106 32-33 -0.15812096108550833 1106 34-35 -0.1842240052196047 1106 36-37 -0.5072261481795692 1106 38-39 -0.37103972940389696 1106 40-41 -0.260684401792723 1106 42-43 -1.0960052078426088 1106 44-45 -1.3348223147701574 1106 46-47 -0.6522037377719556 1106 48-49 -0.9037340611220159 1106 50-51 -1.014770221387586 1106 52-53 -1.2222376594930893 1106 54-55 -0.9577139399275616 1106 56-57 -1.2342686149648046 1106 58-59 -1.3208905771003892 1106 60-61 -0.9066861167024811 1106 62-63 -0.7139489006613324 1106 64-65 -1.0958147867117631 1106 66-67 -0.9379546913122567 1106 68-69 -0.8571118778886202 1106 70-71 -0.7914869570988081 1106 72-73 -0.46238172812621414 1106 74-75 -0.40049069138584414 1106 76-77 -0.35718662027421644 1106 78-79 -0.3141527590722397 1106 80-81 -0.3889251823726134 1106 82-83 -0.3742454319120512 1106 84-85 -0.5095285819211028 1106 86-87 -0.2744210258202351 1106 88-89 0.1447553919086637 1106 90-91 0.05300347562071295 1106 92-93 0.28603517998722694 1106 94-95 0.45302483037893637 1106 96-97 0.35477423258289065 1106 98-99 0.14453612042645503 1106 100-101 0.006701004837385227 1107 1 0.16828814905212042 1107 2 0.20788895808071217 1107 3 0.12565370433762624 1107 4 0.01571570712166448 1107 5 0.011807650728769659 1107 6 0.07776744750959352 1107 7 0.20382422555476154 1107 8 -0.1349570072433366 1107 9 0.19841338821310472 1107 10-11 0.03359538168238885 1107 12-13 0.10898233581381334 1107 14-15 0.14401954606767475 1107 16-17 0.17242529926334527 1107 18-19 0.09568070250872296 1107 20-21 0.12621349099367052 1107 22-23 0.12099405836072208 1107 24-25 -0.04084366137883677 1107 26-27 0.1471207656441038 1107 28-29 0.4830796478356234 1107 30-31 0.380980154423348 1107 32-33 0.5130837601192084 1107 34-35 0.4777825067869088 1107 36-37 0.18693339597997038 1107 38-39 0.2926965343323644 1107 40-41 0.26642182531350045 1107 42-43 0.21447505263764555 1107 44-45 0.2169888370409936 1107 46-47 0.4206696851014655 1107 48-49 0.7295382221502678 1107 50-51 0.4730604664431013 1107 52-53 0.4071884699330397 1107 54-55 0.3026645704509505 1107 56-57 0.46129508059889446 1107 58-59 0.5356010793912667 1107 60-61 0.5736557636394082 1107 62-63 0.7200763333639024 1107 64-65 0.4686133777163981 1107 66-67 0.9359769326193685 1107 68-69 0.992908472131731 1107 70-71 0.7253628597510122 1107 72-73 0.8146227488782607 1107 74-75 0.9730135821184298 1107 76-77 0.8999358868482865 1107 78-79 1.380902185982709 1107 80-81 1.5348436413962112 1107 82-83 1.4877407300741154 1107 84-85 1.338639916247395 1107 86-87 1.3635724621732486 1107 88-89 1.357535204688478 1107 90-91 1.1469188195360545 1107 92-93 1.2926489366009797 1107 94-95 1.4365413138954182 1107 96-97 1.6374767152853735 1107 98-99 1.321235347125679 1107 100-101 1.1990697472061278 1108 1 0.3301528468543147 1108 2 0.34653937016862457 1108 3 0.329757413128835 1108 4 0.6193300615172674 1108 5 0.4154649309485521 1108 6 0.5084937524546476 1108 7 0.5537212035767425 1108 8 0.6249331026467715 1108 9 0.550851575026293 1108 10-11 0.7341233693197466 1108 12-13 0.8180654402094163 1108 14-15 0.5718569430456952 1108 16-17 0.6920595712413657 1108 18-19 0.8548196104757508 1108 20-21 0.6642715267079566 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2316 64-65 -0.8001396084278909 2316 66-67 -0.6871955470748503 2316 68-69 -0.7045353883556089 2316 70-71 -0.6007002076164056 2316 72-73 -0.5328211116090777 2316 74-75 -0.19576329318018182 2316 76-77 -0.21473205390660866 2316 78-79 0.04485345210788694 2316 80-81 -0.11871584259805346 2316 82-83 -0.07936390442182528 2316 84-85 -0.4690523914449116 2316 86-87 -0.4088355645825992 2316 88-89 -0.5049110447142944 2316 90-91 0.02331633506400621 2316 92-93 -0.05051876526237109 2316 94-95 0.33563352603110985 2316 96-97 0.4722115456723799 2316 98-99 0.47923821382419973 2316 100-101 0.6237472436227556 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1501.0 3 241.0 4 219.0 5 251.0 6 271.0 7 346.0 8 480.0 9 571.0 10 848.0 11 1329.0 12 2097.0 13 3227.0 14 3637.0 15 5027.0 16 6981.0 17 9892.0 18 7731.0 19 4489.0 20 4072.0 21 4054.0 22 4268.0 23 4751.0 24 5034.0 25 5585.0 26 6170.0 27 7062.0 28 7996.0 29 9416.0 30 11487.0 31 13648.0 32 17482.0 33 22752.0 34 30481.0 35 43412.0 36 56354.0 37 70471.0 38 49214.0 39 4933.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.99246793482209 18.499961331961597 15.471668045436115 20.035902687780197 2 14.991495682243078 21.201053317152482 41.27522596183131 22.53222503877313 3 18.420824518616914 27.20742755533065 32.02793532985196 22.343812596200475 4 11.55538375035142 17.342563958391903 38.50412332489926 32.597928966357415 5 11.999953143486358 38.07654011503274 34.171518268183256 15.751988473297645 6 28.557877873751664 37.34237694628169 18.225956043544247 15.8737891364224 7 25.533544440176502 36.03275278951461 21.168457639663103 17.26524513064578 8 26.32247093901585 32.36514134072848 20.716622906356058 20.595764813899617 9 27.59223255487105 15.803096671429575 21.071654447073154 35.533016326626225 10-11 23.96144092860364 27.181685612382942 27.266136069481288 21.590737389532126 12-13 26.661692725200663 25.513625827640045 28.28224502010464 19.54243642705465 14-15 22.97512531034806 25.450531690635685 29.487398697709278 22.086944301306975 16-17 19.640878866589066 29.209830452104303 29.507282900700066 21.64200778060657 18-19 19.27808892391316 25.925661379068888 35.36757723261176 19.428672464406198 20-21 20.62139251223428 24.714943328262798 35.322201573154494 19.341462586348428 22-23 20.10740436713745 25.07187029037354 35.366974679408735 19.453750663080278 24-25 19.93254953393602 25.115579184036722 35.637149280996766 19.314722001030493 26-27 19.650654510495936 25.34446842319118 35.63361463799053 19.371262428322346 28-29 19.173128249089757 25.75100158919209 35.80179249624953 19.274077665468624 30-31 19.090116932763664 25.827688141818456 35.57559871823701 19.50659620718087 32-33 19.01172656823673 26.02283946207457 35.18719997001896 19.77823399966974 34-35 19.69607540028294 25.8198890731424 34.81911146088048 19.664924065694183 36-37 19.85729977352516 25.533222945390754 34.91061621297534 19.698861068108737 38-39 19.570606385283288 25.835280066042543 34.95406881773312 19.640044730941046 40-41 19.432868432361417 26.074305312423522 34.892279661880266 19.600546593334794 42-43 19.44670187477312 26.389685820344976 33.999906319894144 20.16370598498776 44-45 19.419460678525386 26.82485576681003 33.561865997501165 20.193817557163417 46-47 19.842164863823573 26.937719646481145 32.55903008329732 20.661085406397955 48-49 20.113737104739553 27.27395160317555 31.857631874495695 20.7546794175892 50-51 20.147996842359237 27.65477241796107 31.564461081140593 20.6327696585391 52-53 20.290883790555235 27.808251429294295 31.103622518720503 20.797242261429965 54-55 19.592356807247825 27.518023525443734 31.491982517531657 21.397637149776784 56-57 20.158850226928895 28.037662234254316 30.84839557289593 20.955091965920854 58-59 20.377280726507184 27.184304597223374 31.13918359270261 21.299231083566827 60-61 20.300454311273477 26.847688632850918 32.04849889934898 20.803358156526627 62-63 20.41194138207622 26.973378376004824 31.82077388305689 20.793906358862067 64-65 20.203405151166372 26.972375444787772 32.00376086429626 20.820458539749595 66-67 20.009858976820865 27.276697687706957 31.59193203160493 21.121511303867248 68-69 19.947871049932324 27.21220182755762 31.751471834910937 21.088455287599118 70-71 19.92271843750732 27.49420388281305 31.22456148567949 21.358516194000142 72-73 20.03070261206395 27.09452821373327 31.166898321894994 21.70787085230779 74-75 19.852950647483194 27.287502678574565 31.152963879348803 21.706582794593437 76-77 19.975783806064353 27.02529585209854 31.35212547454606 21.646794867291042 78-79 19.741530837249442 26.826012265340754 31.501807480757094 21.930649416652713 80-81 19.577948197304124 27.122349587983667 31.302936516579607 21.996765698132606 82-83 19.68311698044276 27.13154240134144 31.117228684575988 22.068111933639813 84-85 19.516892714636295 26.621948820741466 30.907298476986195 22.95385998763605 86-87 19.911038153602615 26.521706550017914 30.886433728224954 22.680821568154517 88-89 19.854595952854336 26.03198467508319 31.031434345705204 23.081985026357266 90-91 19.474445922401035 26.072910616932386 31.76660827697323 22.686035183693352 92-93 19.659315657029907 26.204497258154984 31.56816204462129 22.56802504019382 94-95 19.373580990809447 26.249928281123942 31.136647584566802 23.23984314349981 96-97 19.52955117899093 26.339443063350636 31.03187028465105 23.09913547300739 98-99 19.940397079573994 26.404445042945717 30.25849400165108 23.3966638758292 100-101 20.42578901641379 25.515023921652197 30.975319599362088 23.083867462571924 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1019.0 1 681.0 2 424.0 3 495.5 4 409.5 5 389.0 6 726.0 7 1638.0 8 2733.0 9 3079.0 10 2518.0 11 1871.0 12 1714.5 13 1782.5 14 1827.0 15 1787.0 16 1754.5 17 1819.5 18 1853.5 19 1949.0 20 2035.5 21 2122.0 22 2357.0 23 2656.5 24 3007.0 25 3401.5 26 3831.5 27 4322.0 28 4855.0 29 5390.5 30 5970.0 31 6655.0 32 7378.5 33 8012.0 34 8913.5 35 9635.0 36 10250.5 37 11128.0 38 11587.5 39 11478.0 40 11488.5 41 12318.0 42 13303.5 43 13943.0 44 14463.5 45 14790.0 46 14816.0 47 15732.5 48 18825.5 49 18519.0 50 15007.5 51 12994.0 52 11713.5 53 11329.5 54 10908.5 55 9891.0 56 8781.5 57 7628.0 58 6719.5 59 6100.5 60 5357.0 61 4549.5 62 3748.5 63 2768.5 64 1839.5 65 1370.5 66 1010.5 67 750.0 68 535.0 69 376.5 70 301.5 71 224.5 72 181.0 73 147.0 74 123.0 75 98.0 76 67.0 77 41.5 78 23.5 79 13.0 80 12.0 81 8.0 82 5.5 83 4.5 84 2.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2503623357800739 2 0.21927158819954182 3 0.21903782318013934 4 0.2197391182383468 5 0.22090794333535932 6 0.18981719575482725 7 0.19262237598765722 8 0.19472626116227967 9 0.20337556688017205 10-11 0.2109729300107532 12-13 0.20770021973911826 14-15 0.19636261629809715 16-17 0.19192108092944973 18-19 0.18105100752723363 20-21 0.18783019308990603 22-23 0.18596007293468605 24-25 0.18701201552199728 26-27 0.1826873626630511 28-29 0.1948431436719809 30-31 0.20290803684136707 32-33 0.1971807938660059 34-35 0.1944924961428772 36-37 0.1872457805413998 38-39 0.1829211276824536 40-41 0.18034971246902615 42-43 0.1858431904249848 44-45 0.1824535976436486 46-47 0.19063537332273597 48-49 0.19414184861377343 50-51 0.2054794520547945 52-53 0.21237552012716818 54-55 0.19589508625929217 56-57 0.18093412501753237 58-59 0.17544064706157372 60-61 0.17766141474589742 62-63 0.18175230258544112 64-65 0.17544064706157372 66-67 0.17719388470709244 68-69 0.17812894478470243 70-71 0.17999906493992238 72-73 0.1824535976436486 74-75 0.1824535976436486 76-77 0.1853756603861798 78-79 0.18806395810930854 80-81 0.18572630791528355 82-83 0.18163542007573985 84-85 0.17111599420262752 86-87 0.17368740941605498 88-89 0.1783627098041049 90-91 0.1785964748235074 92-93 0.1838561877600636 94-95 0.17894712235261118 96-97 0.1831548927018561 98-99 0.18408995277946608 100-101 0.17789517976529992 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 427780.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.65719792277224 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25780665897952 45.47182436725391 2 11.229289885079126 12.72440199102225 3 3.6615805440009885 6.223646807749081 4 1.5583575997807873 3.531686998609455 5 0.8282404802949741 2.346289240986215 6 0.5410605584390482 1.8392985088612097 7 0.33795478359949727 1.3403299744341064 8 0.23965063795865832 1.0862346901713873 9 0.16812707121689918 0.8573047875100693 >10 1.0464606966426542 11.264340990166229 >50 0.0811906067401255 3.1621135570864185 >100 0.04409845137412857 4.361624511170683 >500 0.0041213505957129505 1.5446301269550866 >1k 0.0012364051787138852 1.4255429969857603 >5k 8.242701191425901E-4 2.820730451038163 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6985 1.6328486605264387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5095 1.1910327738557203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3799 0.8880733087100846 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1172 0.273972602739726 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1134 0.2650895320024312 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 976 0.22815465893683667 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 788 0.18420683528916731 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 773 0.1807003599981299 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 679 0.1587264481742952 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 615 0.1437654869325354 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 595 0.1390901865444855 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 581 0.13581747627285054 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 548 0.12810323063256815 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 543 0.12693440553555566 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.12085651503109074 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 483 0.11290850437140586 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 437 0.10215531347889102 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 430 0.10051895834307355 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.337650194024966E-4 0.0 24-25 0.0 0.0 0.0 0.0014025901164149797 0.0 26-27 0.0 0.0 0.0 0.002337650194024966 0.0 28-29 0.0 0.0 0.0 0.005610360465659919 0.0 30-31 0.0 0.0 0.0 0.010753190892514844 0.0 32-33 0.0 0.0 0.0 0.01601290382907102 0.0 34-35 0.0 0.0 0.0 0.023376501940249664 0.0 36-37 0.0 0.0 0.0 0.028519332367104588 0.0 38-39 0.0 0.0 0.0 0.03740240310439946 0.0 40-41 0.0 0.0 0.0 0.04909065407452429 0.0 42-43 0.0 0.0 0.0 0.07176586095656647 0.0 44-45 0.0 0.0 0.0 0.10601243629903222 0.0 46-47 0.0 0.0 0.0 0.14388236944223665 0.0 48-49 0.0 0.0 0.0 0.24779092056664642 0.0 50-51 0.0 0.0 0.0 0.34036186825003506 0.0 52-53 0.0 0.0 0.0 0.4404132965543036 0.0 54-55 0.0 0.0 0.0 0.6157370611061761 0.0 56-57 0.0 0.0 0.0 0.7904764131095423 0.0 58-59 0.0 0.0 0.0 1.0216700172986115 0.0 60-61 0.0 0.0 0.0 1.2720323530786852 0.0 62-63 0.0 0.0 0.0 1.6095890410958904 0.0 64-65 0.0 0.0 0.0 1.8635747346767029 0.0 66-67 0.0 0.0 0.0 2.2159755014259668 0.0 68-69 0.0 0.0 0.0 2.669245874047408 0.0 70-71 0.0 0.0 0.0 2.9724391042124454 0.0 72-73 0.0 0.0 0.0 3.4835663191360045 0.0 74-75 0.0 0.0 0.0 4.137523960914489 0.0 76-77 0.0 0.0 0.0 4.6474823507410346 0.0 78-79 0.0 0.0 0.0 5.127168170554958 0.0 80-81 0.0 0.0 0.0 5.785684230211791 0.0 82-83 0.0 0.0 0.0 6.563303567254196 0.0 84-85 0.0 0.0 0.0 7.326195708074243 0.0 86-87 0.0 0.0 0.0 8.36808639955117 0.0 88-89 0.0 0.0 0.0 9.628313619150031 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTATCG 50 0.0016127441 38.009274 9 GTATCAA 5440 0.0 35.188766 1 GGTATCA 3450 0.0 32.630756 1 TAGGGTG 65 0.0058363928 29.234478 5 TACACGG 65 0.0058363928 29.234478 4 TATCAAC 6815 0.0 28.092342 2 GTACACG 85 6.3957873E-4 27.944725 3 ATCAACG 6895 0.0 27.766396 3 TCAACGC 6900 0.0 27.746275 4 CAACGCA 6980 0.0 27.428267 5 AACGCAG 7075 0.0 27.123941 6 GGACACG 160 6.221853E-8 26.719013 6 GTACATG 3105 0.0 26.15968 1 CACGGTG 110 9.4472736E-5 25.915415 9 AGTACTT 4550 0.0 24.898598 12-13 TACATGG 3220 0.0 24.785755 2 ACGCAGA 7660 0.0 24.748205 7 GAGTACT 4455 0.0 24.62987 12-13 CGCAGAG 7705 0.0 24.603668 8 TTAGGGT 215 1.6516424E-9 24.30541 4 >>END_MODULE